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Protein

Protein IscX

Gene

iscX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May function as iron donor in the assembly of iron-sulfur clusters.

GO - Molecular functioni

  • enzyme inhibitor activity Source: EcoCyc
  • ferrous iron binding Source: EcoCyc
  • iron ion binding Source: EcoCyc

GO - Biological processi

  • iron-sulfur cluster assembly Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:EG12311-MONOMER.
ECOL316407:JW2508-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein IscX
Gene namesi
Name:iscX
Synonyms:yfhJ
Ordered Locus Names:b2524, JW2508
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12311. iscX.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6666Protein IscXPRO_0000211853Add
BLAST

Proteomic databases

PaxDbiP0C0L9.
PRIDEiP0C0L9.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4260583. 8 interactions.
DIPiDIP-48188N.
IntActiP0C0L9. 5 interactions.
STRINGi511145.b2524.

Structurei

Secondary structure

1
66
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 1811Combined sources
Helixi24 – 263Combined sources
Helixi29 – 379Combined sources
Beta strandi40 – 423Combined sources
Helixi46 – 483Combined sources
Helixi51 – 6414Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UJ8X-ray1.75A2-66[»]
2BZTNMR-A1-66[»]
ProteinModelPortaliP0C0L9.
SMRiP0C0L9. Positions 1-66.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0C0L9.

Family & Domainsi

Sequence similaritiesi

Belongs to the IscX family.Curated

Phylogenomic databases

eggNOGiENOG4105WVQ. Bacteria.
COG2975. LUCA.
HOGENOMiHOG000262066.
InParanoidiP0C0L9.
OMAiAIQQAWI.
OrthoDBiEOG6ZD6DN.
PhylomeDBiP0C0L9.

Family and domain databases

Gene3Di1.10.10.600. 1 hit.
InterProiIPR007479. ISC_FeS_clus_asmbl_IscsX.
[Graphical view]
PfamiPF04384. Fe-S_assembly. 1 hit.
[Graphical view]
PIRSFiPIRSF039003. IscX. 1 hit.
SUPFAMiSSF140319. SSF140319. 1 hit.
TIGRFAMsiTIGR03412. iscX_yfhJ. 1 hit.

Sequencei

Sequence statusi: Complete.

P0C0L9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLKWTDSRE IGEALYDAYP DLDPKTVRFT DMHQWICDLE DFDDDPQASN
60
EKILEAILLV WLDEAE
Length:66
Mass (Da):7,732
Last modified:November 22, 2005 - v1
Checksum:i31C176F0C906AAD7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti43 – 519DDDPQASNE → RTDPGIPTK (PubMed:8300516).Curated
Sequence conflicti55 – 551E → R (PubMed:8300516).Curated
Sequence conflicti58 – 669LLVWLDEAE → C (PubMed:8300516).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88654 Genomic DNA. No translation available.
U01827 Unassigned DNA. No translation available.
U00096 Genomic DNA. Translation: AAC75577.1.
AP009048 Genomic DNA. Translation: BAA16414.1.
PIRiC65029.
RefSeqiNP_417019.1. NC_000913.3.
WP_000523616.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75577; AAC75577; b2524.
BAA16414; BAA16414; BAA16414.
GeneIDi945196.
KEGGiecj:JW2508.
eco:b2524.
PATRICi32120443. VBIEscCol129921_2624.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88654 Genomic DNA. No translation available.
U01827 Unassigned DNA. No translation available.
U00096 Genomic DNA. Translation: AAC75577.1.
AP009048 Genomic DNA. Translation: BAA16414.1.
PIRiC65029.
RefSeqiNP_417019.1. NC_000913.3.
WP_000523616.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UJ8X-ray1.75A2-66[»]
2BZTNMR-A1-66[»]
ProteinModelPortaliP0C0L9.
SMRiP0C0L9. Positions 1-66.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260583. 8 interactions.
DIPiDIP-48188N.
IntActiP0C0L9. 5 interactions.
STRINGi511145.b2524.

Proteomic databases

PaxDbiP0C0L9.
PRIDEiP0C0L9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75577; AAC75577; b2524.
BAA16414; BAA16414; BAA16414.
GeneIDi945196.
KEGGiecj:JW2508.
eco:b2524.
PATRICi32120443. VBIEscCol129921_2624.

Organism-specific databases

EchoBASEiEB2217.
EcoGeneiEG12311. iscX.

Phylogenomic databases

eggNOGiENOG4105WVQ. Bacteria.
COG2975. LUCA.
HOGENOMiHOG000262066.
InParanoidiP0C0L9.
OMAiAIQQAWI.
OrthoDBiEOG6ZD6DN.
PhylomeDBiP0C0L9.

Enzyme and pathway databases

BioCyciEcoCyc:EG12311-MONOMER.
ECOL316407:JW2508-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0C0L9.
PROiP0C0L9.

Family and domain databases

Gene3Di1.10.10.600. 1 hit.
InterProiIPR007479. ISC_FeS_clus_asmbl_IscsX.
[Graphical view]
PfamiPF04384. Fe-S_assembly. 1 hit.
[Graphical view]
PIRSFiPIRSF039003. IscX. 1 hit.
SUPFAMiSSF140319. SSF140319. 1 hit.
TIGRFAMsiTIGR03412. iscX_yfhJ. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing, and overexpression of a [2Fe-2S] ferredoxin gene from Escherichia coli."
    Ta D.T., Vickery L.E.
    J. Biol. Chem. 267:11120-11125(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Mutations in a gene encoding a new Hsp70 suppress rapid DNA inversion and bgl activation, but not proU derepression, in hns-1 mutant Escherichia coli."
    Kawula T.H., Lelivelt M.J.
    J. Bacteriol. 176:610-619(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Crystal structure of Escherichia coli YfhJ protein, a member of the ISC machinery involved in assembly of iron-sulfur clusters."
    Shimomura Y., Takahashi Y., Kakuta Y., Fukuyama K.
    Proteins 60:566-569(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 2-66, SUBUNIT.

Entry informationi

Entry nameiISCX_ECOLI
AccessioniPrimary (citable) accession number: P0C0L9
Secondary accession number(s): P37096
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: July 6, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.