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Protein

Proline/betaine transporter

Gene

proP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in the bacterial osmoregulatory response.3 Publications

Kineticsi

  1. KM=175 µM for proline1 Publication
  1. Vmax=83.6 nmol/min/mg enzyme with proline as substrate1 Publication

GO - Molecular functioni

  • glycine betaine:proton symporter activity Source: EcoCyc

GO - Biological processi

  • amino acid transport Source: EcoliWiki
Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:PROP-MONOMER.
ECOL316407:JW4072-MONOMER.
MetaCyc:PROP-MONOMER.

Protein family/group databases

TCDBi2.A.1.6.4. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Proline/betaine transporter
Alternative name(s):
Proline porter II
Short name:
PPII
Gene namesi
Name:proP
Ordered Locus Names:b4111, JW4072
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11612. proP.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3737CytoplasmicSequence analysisAdd
BLAST
Transmembranei38 – 5821Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini59 – 657PeriplasmicSequence analysis
Transmembranei66 – 8621Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini87 – 9711CytoplasmicSequence analysisAdd
BLAST
Transmembranei98 – 11821Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini119 – 1213PeriplasmicSequence analysis
Transmembranei122 – 14221Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini143 – 16927CytoplasmicSequence analysisAdd
BLAST
Transmembranei170 – 19021Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini191 – 1944PeriplasmicSequence analysis
Transmembranei195 – 21521Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini216 – 26045CytoplasmicSequence analysisAdd
BLAST
Transmembranei261 – 28121Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini282 – 29716PeriplasmicSequence analysisAdd
BLAST
Transmembranei298 – 31821Helical; Name=8Sequence analysisAdd
BLAST
Topological domaini319 – 3257CytoplasmicSequence analysis
Transmembranei326 – 34621Helical; Name=9Sequence analysisAdd
BLAST
Topological domaini347 – 3504PeriplasmicSequence analysis
Transmembranei351 – 37121Helical; Name=10Sequence analysisAdd
BLAST
Topological domaini372 – 39019CytoplasmicSequence analysisAdd
BLAST
Transmembranei391 – 41121Helical; Name=11Sequence analysisAdd
BLAST
Topological domaini412 – 4165PeriplasmicSequence analysis
Transmembranei417 – 43721Helical; Name=12Sequence analysisAdd
BLAST
Topological domaini438 – 50063Cytoplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: EcoliWiki
  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 500500Proline/betaine transporterPRO_0000050324Add
BLAST

Proteomic databases

PaxDbiP0C0L7.

Expressioni

Inductioni

Induced by an increase in the osmolarity of the growth medium.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4263085. 4 interactions.
STRINGi511145.b4111.

Structurei

Secondary structure

1
500
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi469 – 49426Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R48NMR-A/B468-497[»]
1Y8Smodel-A1-500[»]
ProteinModelPortaliP0C0L7.
SMRiP0C0L7. Positions 25-446, 468-497.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0C0L7.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili453 – 49846Sequence analysisAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CSH. Bacteria.
ENOG410XP7I. LUCA.
HOGENOMiHOG000141611.
InParanoidiP0C0L7.
KOiK03762.
OMAiDYMVLSY.
PhylomeDBiP0C0L7.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR004736. Cit_H_symport.
IPR020846. MFS_dom.
IPR005828. MFS_sugar_transport_like.
IPR015041. Osmo_CC.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF08946. Osmo_CC. 1 hit.
PF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00883. 2A0106. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C0L7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKRKKVKPI TLRDVTIIDD GKLRKAITAA SLGNAMEWFD FGVYGFVAYA
60 70 80 90 100
LGKVFFPGAD PSVQMVAALA TFSVPFLIRP LGGLFFGMLG DKYGRQKILA
110 120 130 140 150
ITIVIMSIST FCIGLIPSYD TIGIWAPILL LICKMAQGFS VGGEYTGASI
160 170 180 190 200
FVAEYSPDRK RGFMGSWLDF GSIAGFVLGA GVVVLISTIV GEANFLDWGW
210 220 230 240 250
RIPFFIALPL GIIGLYLRHA LEETPAFQQH VDKLEQGDRE GLQDGPKVSF
260 270 280 290 300
KEIATKYWRS LLTCIGLVIA TNVTYYMLLT YMPSYLSHNL HYSEDHGVLI
310 320 330 340 350
IIAIMIGMLF VQPVMGLLSD RFGRRPFVLL GSVALFVLAI PAFILINSNV
360 370 380 390 400
IGLIFAGLLM LAVILNCFTG VMASTLPAMF PTHIRYSALA AAFNISVLVA
410 420 430 440 450
GLTPTLAAWL VESSQNLMMP AYYLMVVAVV GLITGVTMKE TANRPLKGAT
460 470 480 490 500
PAASDIQEAK EILVEHYDNI EQKIDDIDHE IADLQAKRTR LVQQHPRIDE
Length:500
Mass (Da):54,846
Last modified:November 8, 2005 - v1
Checksum:i60EDDBF61190DDFC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83089 Genomic DNA. Translation: AAB00919.1.
U14003 Genomic DNA. Translation: AAA97010.1.
U00096 Genomic DNA. Translation: AAC77072.1.
AP009048 Genomic DNA. Translation: BAE78113.1.
PIRiS32331.
RefSeqiNP_418535.1. NC_000913.3.
WP_001298520.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77072; AAC77072; b4111.
BAE78113; BAE78113; BAE78113.
GeneIDi948626.
KEGGiecj:JW4072.
eco:b4111.
PATRICi32123787. VBIEscCol129921_4242.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83089 Genomic DNA. Translation: AAB00919.1.
U14003 Genomic DNA. Translation: AAA97010.1.
U00096 Genomic DNA. Translation: AAC77072.1.
AP009048 Genomic DNA. Translation: BAE78113.1.
PIRiS32331.
RefSeqiNP_418535.1. NC_000913.3.
WP_001298520.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1R48NMR-A/B468-497[»]
1Y8Smodel-A1-500[»]
ProteinModelPortaliP0C0L7.
SMRiP0C0L7. Positions 25-446, 468-497.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263085. 4 interactions.
STRINGi511145.b4111.

Protein family/group databases

TCDBi2.A.1.6.4. the major facilitator superfamily (mfs).

Proteomic databases

PaxDbiP0C0L7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77072; AAC77072; b4111.
BAE78113; BAE78113; BAE78113.
GeneIDi948626.
KEGGiecj:JW4072.
eco:b4111.
PATRICi32123787. VBIEscCol129921_4242.

Organism-specific databases

EchoBASEiEB1569.
EcoGeneiEG11612. proP.

Phylogenomic databases

eggNOGiENOG4105CSH. Bacteria.
ENOG410XP7I. LUCA.
HOGENOMiHOG000141611.
InParanoidiP0C0L7.
KOiK03762.
OMAiDYMVLSY.
PhylomeDBiP0C0L7.

Enzyme and pathway databases

BioCyciEcoCyc:PROP-MONOMER.
ECOL316407:JW4072-MONOMER.
MetaCyc:PROP-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0C0L7.
PROiP0C0L7.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR004736. Cit_H_symport.
IPR020846. MFS_dom.
IPR005828. MFS_sugar_transport_like.
IPR015041. Osmo_CC.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF08946. Osmo_CC. 1 hit.
PF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00883. 2A0106. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPROP_ECOLI
AccessioniPrimary (citable) accession number: P0C0L7
Secondary accession number(s): P30848, Q2M6J3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: September 7, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.