ID   NPSR1_RAT               Reviewed;         372 AA.
AC   P0C0L6;
DT   08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   27-MAR-2024, entry version 109.
DE   RecName: Full=Neuropeptide S receptor;
DE   AltName: Full=G-protein coupled receptor 154;
GN   Name=Npsr1; Synonyms=Gpr154;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Brown Norway;
RX   PubMed=15057822; DOI=10.1038/nature02426;
RA   Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA   Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA   Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA   Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA   Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA   Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA   Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA   Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA   Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA   Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA   Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA   Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA   Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA   Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA   Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA   Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA   Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA   Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA   Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA   Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA   Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA   Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA   Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA   Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA   Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA   Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA   Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA   Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA   Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA   Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA   Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA   Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA   Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA   Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA   Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA   Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA   Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA   Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA   Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA   Mockrin S., Collins F.S.;
RT   "Genome sequence of the Brown Norway rat yields insights into mammalian
RT   evolution.";
RL   Nature 428:493-521(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-103.
RG   Amgen EST program;
RT   "Amgen rat EST program.";
RL   Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: G-protein coupled receptor for neuropeptide S (NPS). Promotes
CC       mobilization of intracellular Ca(2+) stores. Inhibits cell growth in
CC       response to NPS binding. Involved in pathogenesis of asthma and other
CC       IgE-mediated diseases. {ECO:0000250|UniProtKB:Q6W5P4}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q6W5P4};
CC       Multi-pass membrane protein {ECO:0000250|UniProtKB:Q6W5P4}.
CC   -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC       Vasopressin/oxytocin receptor subfamily. {ECO:0000255|PROSITE-
CC       ProRule:PRU00521}.
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DR   EMBL; AABR03062350; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AABR03063281; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AABR03064185; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CB812328; -; NOT_ANNOTATED_CDS; mRNA.
DR   AlphaFoldDB; P0C0L6; -.
DR   SMR; P0C0L6; -.
DR   STRING; 10116.ENSRNOP00000021625; -.
DR   ChEMBL; CHEMBL3308949; -.
DR   GuidetoPHARMACOLOGY; 302; -.
DR   GlyCosmos; P0C0L6; 2 sites, No reported glycans.
DR   GlyGen; P0C0L6; 2 sites.
DR   PhosphoSitePlus; P0C0L6; -.
DR   PaxDb; 10116-ENSRNOP00000021625; -.
DR   Ensembl; ENSRNOT00000021625.7; ENSRNOP00000021625.7; ENSRNOG00000015863.7.
DR   Ensembl; ENSRNOT00055022701; ENSRNOP00055018443; ENSRNOG00055013231.
DR   Ensembl; ENSRNOT00060043243; ENSRNOP00060035799; ENSRNOG00060024996.
DR   Ensembl; ENSRNOT00065024786; ENSRNOP00065019370; ENSRNOG00065014978.
DR   UCSC; RGD:1564154; rat.
DR   AGR; RGD:1564154; -.
DR   RGD; 1564154; Npsr1.
DR   eggNOG; KOG3184; Eukaryota.
DR   eggNOG; KOG3656; Eukaryota.
DR   GeneTree; ENSGT00940000155094; -.
DR   HOGENOM; CLU_009579_15_0_1; -.
DR   InParanoid; P0C0L6; -.
DR   OMA; NRLCPPF; -.
DR   PhylomeDB; P0C0L6; -.
DR   TreeFam; TF106499; -.
DR   Reactome; R-RNO-375276; Peptide ligand-binding receptors.
DR   Reactome; R-RNO-416476; G alpha (q) signalling events.
DR   PRO; PR:P0C0L6; -.
DR   Proteomes; UP000002494; Chromosome 8.
DR   GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR   GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR   GO; GO:0008188; F:neuropeptide receptor activity; ISS:UniProtKB.
DR   GO; GO:0005000; F:vasopressin receptor activity; IEA:InterPro.
DR   GO; GO:0042755; P:eating behavior; IMP:RGD.
DR   GO; GO:2000293; P:negative regulation of defecation; IMP:RGD.
DR   GO; GO:1903999; P:negative regulation of eating behavior; IMP:RGD.
DR   GO; GO:0007218; P:neuropeptide signaling pathway; ISS:UniProtKB.
DR   GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IMP:RGD.
DR   GO; GO:0051281; P:positive regulation of release of sequestered calcium ion into cytosol; ISS:UniProtKB.
DR   GO; GO:0060013; P:righting reflex; IMP:RGD.
DR   CDD; cd15197; 7tmA_NPSR; 1.
DR   Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1.
DR   InterPro; IPR000276; GPCR_Rhodpsn.
DR   InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR   InterPro; IPR027294; NPS_rcpt.
DR   InterPro; IPR001817; Vasoprsn_rcpt.
DR   PANTHER; PTHR24244; NEUROPEPTIDE S RECEPTOR; 1.
DR   PANTHER; PTHR24244:SF2; NEUROPEPTIDE S RECEPTOR; 1.
DR   Pfam; PF00001; 7tm_1; 1.
DR   PRINTS; PR00237; GPCRRHODOPSN.
DR   PRINTS; PR00896; VASOPRESSINR.
DR   SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1.
DR   PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR   PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW   Membrane; Receptor; Reference proteome; Transducer; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..372
FT                   /note="Neuropeptide S receptor"
FT                   /id="PRO_0000069642"
FT   TOPO_DOM        1..52
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        53..73
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        74..82
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        83..103
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        104..122
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        123..143
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        144..165
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        166..186
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        187..213
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        214..234
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        235..276
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        277..297
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        298..313
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        314..334
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        335..372
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        4
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        13
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        121..198
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
SQ   SEQUENCE   372 AA;  42638 MW;  896230261D77BDDE CRC64;
     MPANLTEGSF HANQTVPMLD SSPVACTEIV TFTEALEAEE WGSFYSSFKT EQLITLWVLF
     VFTIVGNSVV LFSTWRRKRK SRMTFFVTQL AITDSFTGLI NILTDIIWRF TGDFMAPDLV
     CRIVRYLQVV LLYASTYVLV SLSIDRYHAI VYPMKFLQGA EKQAKVLIGI AWSLSFLFSI
     PTLIIFGKRT LSNGEVQCWA LWPDDSYWTP YMTIVAFLVY FIPLTIISVI YGLVIRTIWI
     KSKAHETVIS NCSDGELCCS YNRGLISKAK IKAIKYSIVI ILAFICCWSP YFLFDMLDNF
     NLLPDTKERF YASVIIQNLP ALNSAINPLI YCIFSGSLCS PCKVQRSQDS RMTYRERSER
     HEMQILSKPE FI
//