P0C0L6 (NPSR1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neuropeptide S receptor

Alternative name(s):
G-protein coupled receptor 154

Gene names

Name:	Npsr1
Synonyms:	Gpr154

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be active in signaling pathway in an autocrine or paracrine fashion in several tissues. Receptor for neuropeptide S, it may mediate its action, such as inhibitory effects, on cell growth By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	neuropeptide signaling pathway Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	neuropeptide receptor activity Inferred from electronic annotation. Source: InterPro vasopressin receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Neuropeptide S receptor		PRO_0000069642
Regions
	Topological domain	1 – 52	52	Extracellular Potential
	Transmembrane	53 – 73	21	Helical; Name=1; Potential
	Topological domain	74 – 82	9	Cytoplasmic Potential
	Transmembrane	83 – 103	21	Helical; Name=2; Potential
	Topological domain	104 – 122	19	Extracellular Potential
	Transmembrane	123 – 143	21	Helical; Name=3; Potential
	Topological domain	144 – 165	22	Cytoplasmic Potential
	Transmembrane	166 – 186	21	Helical; Name=4; Potential
	Topological domain	187 – 213	27	Extracellular Potential
	Transmembrane	214 – 234	21	Helical; Name=5; Potential
	Topological domain	235 – 276	42	Cytoplasmic Potential
	Transmembrane	277 – 297	21	Helical; Name=6; Potential
	Topological domain	298 – 313	16	Extracellular Potential
	Transmembrane	314 – 334	21	Helical; Name=7; Potential
	Topological domain	335 – 372	38	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	4	1	N-linked (GlcNAc...) Potential
	Glycosylation	13	1	N-linked (GlcNAc...) Potential
	Disulfide bond	121 ↔ 198		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C0L6 [UniParc]. Last modified November 8, 2005. Version 1. Checksum: 896230261D77BDDE	FASTA	372	42,638
	10 20 30 40 50 60 MPANLTEGSF HANQTVPMLD SSPVACTEIV TFTEALEAEE WGSFYSSFKT EQLITLWVLF 70 80 90 100 110 120 VFTIVGNSVV LFSTWRRKRK SRMTFFVTQL AITDSFTGLI NILTDIIWRF TGDFMAPDLV 130 140 150 160 170 180 CRIVRYLQVV LLYASTYVLV SLSIDRYHAI VYPMKFLQGA EKQAKVLIGI AWSLSFLFSI 190 200 210 220 230 240 PTLIIFGKRT LSNGEVQCWA LWPDDSYWTP YMTIVAFLVY FIPLTIISVI YGLVIRTIWI 250 260 270 280 290 300 KSKAHETVIS NCSDGELCCS YNRGLISKAK IKAIKYSIVI ILAFICCWSP YFLFDMLDNF 310 320 330 340 350 360 NLLPDTKERF YASVIIQNLP ALNSAINPLI YCIFSGSLCS PCKVQRSQDS RMTYRERSER 370 HEMQILSKPE FI

References

[1]	"Genome sequence of the Brown Norway rat yields insights into mammalian evolution." Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M. , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S. Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway.
[2]	"Amgen rat EST program." Amgen EST program Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-103.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AABR03062350 Genomic DNA. No translation available. AABR03063281 Genomic DNA. No translation available. AABR03064185 Genomic DNA. No translation available. CB812328 mRNA. No translation available.
IPI	IPI00362561.
UniGene	Rn.101463.
3D structure databases
ProteinModelPortal	P0C0L6.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000021625.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P0C0L6.
Proteomic databases
PaxDb	P0C0L6.
PRIDE	P0C0L6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000021625; ENSRNOP00000021625; ENSRNOG00000015863.
UCSC	RGD:1564154. rat.
Organism-specific databases
RGD	1564154. Npsr1.
Phylogenomic databases
eggNOG	NOG299951.
GeneTree	ENSGT00660000095405.
HOGENOM	HOG000220789.
HOVERGEN	HBG106615.
InParanoid	P0C0L6.
OMA	GEKQARV.
OrthoDB	EOG4W6NWJ.
Gene expression databases
Genevestigator	P0C0L6.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR027294. NPS_rcpt. IPR001817. Vasoprsn_rcpt. [Graphical view]
PANTHER	PTHR24244:SF1. PTHR24244:SF1. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00896. VASOPRESSINR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NPSR1_RAT

Accession

Primary (citable) accession number: P0C0L6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 8, 2005
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program