P0C0K3 (RDOA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein rdoA

Gene names

Name:	rdoA
Synonyms:	yihE
Ordered Locus Names:	b3859, JW3831

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probably involved in the extracytoplasmic stress response By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the rdoA family.

Ontologies

Keywords
Cellular component	Cytoplasm
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from experiment. Source: EcoCyc
Molecular function	protein serine/threonine kinase activity Inferred from direct assay. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 328

328

Protein rdoA

PRO_0000209574

Secondary structure

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328

Helix Strand Turn

Details...

Helix

13 – 22

10

Beta strand

32 – 34

3

Beta strand

37 – 44

8

Beta strand

52 – 57

6

Turn

59 – 61

3

Helix

64 – 79

16

Beta strand

96 – 98

3

Beta strand

101 – 107

7

Helix

119 – 136

18

Beta strand

142 – 144

3

Helix

149 – 152

4

Helix

154 – 161

8

Beta strand

164 – 166

3

Turn

168 – 170

3

Helix

171 – 188

18

Helix

204 – 206

3

Beta strand

207 – 215

9

Helix

228 – 231

4

Helix

238 – 252

15

Turn

253 – 255

3

Helix

260 – 265

6

Helix

266 – 283

18

Turn

284 – 286

3

Helix

289 – 293

5

Helix

295 – 298

4

Helix

300 – 316

17

Sequences

	Sequence		Length	Mass (Da)
	P0C0K3 [UniParc]. Last modified September 27, 2005. Version 1. Checksum: 100AEEF0FBF04436	FASTA	328	38,120
	10 20 30 40 50 60 MNNSAFTFQT LHPDTIMDAL FEHGIRVDSG LTPLNSYENR VYQFQDEDRR RFVVKFYRPE 70 80 90 100 110 120 RWTADQILEE HQFALQLVND EVPVAAPVAF NGQTLLNHQG FYFAVFPSVG GRQFEADNID 130 140 150 160 170 180 QMEAVGRYLG RMHQTGRKQL FIHRPTIGLN EYLIEPRKLF EDATLIPSGL KAAFLKATDE 190 200 210 220 230 240 LIAAVTAHWR EDFTVLRLHG DCHAGNILWR DGPMFVDLDD ARNGPAVQDL WMLLNGDKAE 250 260 270 280 290 300 QRMQLETIIE AYEEFSEFDT AEIGLIEPLR AMRLVYYLAW LMRRWADPAF PKNFPWLTGE 310 320 DYWLRQTATF IEQAKVLQEP PLQLTPMY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Escherichia coli dsbA gene is partly transcribed from the promoter of a weakly expressed upstream gene." Belin P., Boquet P.L. Microbiology 140:3337-3348(1994) [PubMed: 7881552] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Plunkett G. III, Burland V., Daniels D.L., Blattner F.R. Nucleic Acids Res. 21:3391-3398(1993) [PubMed: 8346018] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X80762 Genomic DNA. Translation: CAA56735.1.
L19201 Genomic DNA. Translation: AAB02994.1.
U00096 Genomic DNA. Translation: AAC76857.1.
AP009048 Genomic DNA. Translation: BAE77449.1.

PIR

S40805.

RefSeq

NP_418296.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZYL	X-ray	2.80	A	1-328	[»]

ProteinModelPortal

P0C0K3.

SMR

P0C0K3. Positions 4-328.

ModBase

Search...

Protein-protein interaction databases

IntAct

P0C0K3. 5 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000001070; EBESCP00000001070; EBESCG00000000886.
EBESCT00000016833; EBESCP00000016124; EBESCG00000015892.

GeneID

948346.

GenomeReviews

Gene locus JW3831 in contig AP009048_GR.
Gene locus b3859 in contig U00096_GR.

KEGG

ecj:JW3831.
eco:b3859.

PATRIC

32123215. VBIEscCol129921_3968.

Organism-specific databases

EchoBASE

EB1778.

EcoGene

EG11831. rdoA.

Phylogenomic databases

eggNOG

COG2334.

GeneTree

EBGT00050000009809.

HOGENOM

HBG307327.

OMA

VQDLWMM.

PhylomeDB

P0C0K3.

ProtClustDB

PRK11768.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11831-MONOMER.
MetaCyc:EG11831-MONOMER.

Gene expression databases

Genevestigator

P0C0K3.

Family and domain databases

InterPro

IPR002575. Aminoglycoside_PTrfase.
IPR011009. Kinase-like_dom.
[Graphical view]

Pfam

PF01636. APH. 1 hit.
[Graphical view]

SUPFAM

SSF56112. Kinase_like. 1 hit.

ProtoNet

Search...

Entry information

Entry name

RDOA_ECOLI

Accession

Primary (citable) accession number: P0C0K3
Secondary accession number(s): P32127, Q2M8F7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	September 27, 2005
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program