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Protein

Stress response kinase A

Gene

srkA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

A protein kinase that (auto)phosphorylates on Ser and Thr residues (PubMed:17302814). Probably acts to suppress the effects of stress linked to accumulation of reactive oxygen species. Protects cells from stress by antagonizing the MazE-MazF TA module, probably indirectly as it has not been seen to phosphorylate MazE, MazF or MazG (PubMed:23416055). Probably involved in the extracytoplasmic stress response (PubMed:9159398).UniRule annotation3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.UniRule annotation1 Publication

Cofactori

Mg2+1 PublicationNote: May bind 2 Mg2+ ions.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei36 – 361ATPUniRule annotation1 Publication
Active sitei201 – 2011Proton acceptorUniRule annotation1 Publication
Metal bindingi206 – 2061MagnesiumUniRule annotation1 Publication
Active sitei217 – 2171UniRule annotation1 Publication
Metal bindingi217 – 2171MagnesiumUniRule annotation1 Publication

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • magnesium ion binding Source: UniProtKB-HAMAP
  • protein serine/threonine kinase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11831-MONOMER.
ECOL316407:JW3831-MONOMER.
MetaCyc:EG11831-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Stress response kinase A1 PublicationUniRule annotation (EC:2.7.11.1UniRule annotation1 Publication)
Alternative name(s):
Serine/threonine protein kinase YihE1 Publication
Serine/threonine-protein kinase SrkAUniRule annotationCurated
Gene namesi
Name:srkA1 PublicationUniRule annotation
Synonyms:rdoA, yihE
Ordered Locus Names:b3859, JW3831
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11831. srkA.

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

A hyperlethal phenotype (reduced survival in the presence of antibiotic but no change in minimal inhibitory concentration) for a number of antimicrobials including nalidixic acid, tetracycline, ampicillin and mitomycin C as well as exposure to UV light and H2O2. Lethality is mitigated by pretreatment with 2,2'-bipyridyl and thiourea, which inhibit hydroxyl radical accumulation. No change in cell survival upon heat shock (up to 55 degrees Celsius), rifampicin or fluoroquinolone PD161144 treatment. A triple srkA-mazE-mazF disruption mutant shows no hyperlethality in the presence of nalidixic acid or UV light, suggesting SrkA has a negative effect on MazF. Double katG-srkA and double cpxR-srkA disruption mutants are as sensitive to killing as single srkA mutants; i.e. all 3 genes are epistatic and function in the same genetic pathway.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi36 – 361S → A: Partial loss of kinase activity, only partially protects bacteria against hyperlethal stress. 1 Publication
Mutagenesisi217 – 2171D → A: Loss of kinase activity; does not protect bacteria against hyperlethal stress. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 328328Stress response kinase APRO_0000209574Add
BLAST

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0C0K3.

Expressioni

Inductioni

Induced by the two-component Cpx system that responds to extracellular stress.1 Publication

Interactioni

Subunit structurei

Monomer.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4261338. 3 interactions.
IntActiP0C0K3. 5 interactions.
STRINGi511145.b3859.

Structurei

Secondary structure

1
328
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi13 – 2210Combined sources
Beta strandi32 – 343Combined sources
Beta strandi37 – 448Combined sources
Beta strandi52 – 576Combined sources
Turni59 – 613Combined sources
Helixi64 – 7916Combined sources
Beta strandi96 – 983Combined sources
Beta strandi101 – 1077Combined sources
Helixi119 – 13618Combined sources
Beta strandi142 – 1443Combined sources
Helixi149 – 1524Combined sources
Helixi154 – 1618Combined sources
Beta strandi164 – 1663Combined sources
Turni168 – 1703Combined sources
Helixi171 – 18818Combined sources
Helixi204 – 2063Combined sources
Beta strandi207 – 2159Combined sources
Helixi228 – 2314Combined sources
Helixi238 – 25215Combined sources
Turni253 – 2553Combined sources
Helixi260 – 2656Combined sources
Helixi266 – 28318Combined sources
Turni284 – 2863Combined sources
Helixi289 – 2935Combined sources
Helixi295 – 2984Combined sources
Helixi300 – 31617Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZYLX-ray2.80A1-328[»]
ProteinModelPortaliP0C0K3.
SMRiP0C0K3. Positions 4-328.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0C0K3.

Family & Domainsi

Sequence similaritiesi

Belongs to the SrkA/RdoA protein kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E27. Bacteria.
COG2334. LUCA.
HOGENOMiHOG000265894.
InParanoidiP0C0K3.
OMAiLHYSAWL.
OrthoDBiEOG6T4RX0.
PhylomeDBiP0C0K3.

Family and domain databases

HAMAPiMF_01497. SrkA_kinase.
InterProiIPR002575. Aminoglycoside_PTrfase.
IPR011009. Kinase-like_dom.
IPR032882. SrkA/RdoA.
[Graphical view]
PANTHERiPTHR39573:SF1. PTHR39573:SF1. 1 hit.
PfamiPF01636. APH. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.

Sequencei

Sequence statusi: Complete.

P0C0K3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNSAFTFQT LHPDTIMDAL FEHGIRVDSG LTPLNSYENR VYQFQDEDRR
60 70 80 90 100
RFVVKFYRPE RWTADQILEE HQFALQLVND EVPVAAPVAF NGQTLLNHQG
110 120 130 140 150
FYFAVFPSVG GRQFEADNID QMEAVGRYLG RMHQTGRKQL FIHRPTIGLN
160 170 180 190 200
EYLIEPRKLF EDATLIPSGL KAAFLKATDE LIAAVTAHWR EDFTVLRLHG
210 220 230 240 250
DCHAGNILWR DGPMFVDLDD ARNGPAVQDL WMLLNGDKAE QRMQLETIIE
260 270 280 290 300
AYEEFSEFDT AEIGLIEPLR AMRLVYYLAW LMRRWADPAF PKNFPWLTGE
310 320
DYWLRQTATF IEQAKVLQEP PLQLTPMY
Length:328
Mass (Da):38,120
Last modified:September 27, 2005 - v1
Checksum:i100AEEF0FBF04436
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80762 Genomic DNA. Translation: CAA56735.1.
L19201 Genomic DNA. Translation: AAB02994.1.
U00096 Genomic DNA. Translation: AAC76857.1.
AP009048 Genomic DNA. Translation: BAE77449.1.
PIRiS40805.
RefSeqiNP_418296.1. NC_000913.3.
WP_001065497.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76857; AAC76857; b3859.
BAE77449; BAE77449; BAE77449.
GeneIDi948346.
KEGGiecj:JW3831.
eco:b3859.
PATRICi32123215. VBIEscCol129921_3968.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80762 Genomic DNA. Translation: CAA56735.1.
L19201 Genomic DNA. Translation: AAB02994.1.
U00096 Genomic DNA. Translation: AAC76857.1.
AP009048 Genomic DNA. Translation: BAE77449.1.
PIRiS40805.
RefSeqiNP_418296.1. NC_000913.3.
WP_001065497.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZYLX-ray2.80A1-328[»]
ProteinModelPortaliP0C0K3.
SMRiP0C0K3. Positions 4-328.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261338. 3 interactions.
IntActiP0C0K3. 5 interactions.
STRINGi511145.b3859.

Proteomic databases

PaxDbiP0C0K3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76857; AAC76857; b3859.
BAE77449; BAE77449; BAE77449.
GeneIDi948346.
KEGGiecj:JW3831.
eco:b3859.
PATRICi32123215. VBIEscCol129921_3968.

Organism-specific databases

EchoBASEiEB1778.
EcoGeneiEG11831. srkA.

Phylogenomic databases

eggNOGiENOG4105E27. Bacteria.
COG2334. LUCA.
HOGENOMiHOG000265894.
InParanoidiP0C0K3.
OMAiLHYSAWL.
OrthoDBiEOG6T4RX0.
PhylomeDBiP0C0K3.

Enzyme and pathway databases

BioCyciEcoCyc:EG11831-MONOMER.
ECOL316407:JW3831-MONOMER.
MetaCyc:EG11831-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0C0K3.
PROiP0C0K3.

Family and domain databases

HAMAPiMF_01497. SrkA_kinase.
InterProiIPR002575. Aminoglycoside_PTrfase.
IPR011009. Kinase-like_dom.
IPR032882. SrkA/RdoA.
[Graphical view]
PANTHERiPTHR39573:SF1. PTHR39573:SF1. 1 hit.
PfamiPF01636. APH. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Escherichia coli dsbA gene is partly transcribed from the promoter of a weakly expressed upstream gene."
    Belin P., Boquet P.L.
    Microbiology 140:3337-3348(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes."
    Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 21:3391-3398(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Regulation of Escherichia coli cell envelope proteins involved in protein folding and degradation by the Cpx two-component system."
    Pogliano J., Lynch A.S., Belin D., Lin E.C., Beckwith J.
    Genes Dev. 11:1169-1182(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION BY CPX.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  6. "YihE kinase is a central regulator of programmed cell death in bacteria."
    Dorsey-Oresto A., Lu T., Mosel M., Wang X., Salz T., Drlica K., Zhao X.
    Cell Rep. 3:528-537(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF SER-36 AND ASP-217.
    Strain: K12 / MG1655 / ATCC 47076.
  7. "Crystal structure of a novel prokaryotic Ser/Thr kinase and its implication in the Cpx stress response pathway."
    Zheng J., He C., Singh V.K., Martin N.L., Jia Z.
    Mol. Microbiol. 63:1360-1371(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS), FUNCTION, CATALYTIC ACTIVITY, POSSIBLE COFACTOR, SUBUNIT, SUBCELLULAR LOCATION, MUTAGENESIS OF ASP-217.

Entry informationi

Entry nameiSRKA_ECOLI
AccessioniPrimary (citable) accession number: P0C0K3
Secondary accession number(s): P32127, Q2M8F7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: September 27, 2005
Last modified: March 16, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.