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Protein

Stress response kinase A

Gene

srkA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

A protein kinase that (auto)phosphorylates on Ser and Thr residues (PubMed:17302814). Probably acts to suppress the effects of stress linked to accumulation of reactive oxygen species. Protects cells from stress by antagonizing the MazE-MazF TA module, probably indirectly as it has not been seen to phosphorylate MazE, MazF or MazG (PubMed:23416055). Probably involved in the extracytoplasmic stress response (PubMed:9159398).UniRule annotation3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.UniRule annotation1 Publication

Cofactori

Mg2+1 PublicationNote: May bind 2 Mg2+ ions.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei36ATPUniRule annotation1 Publication1
Active sitei201Proton acceptorUniRule annotation1 Publication1
Metal bindingi206MagnesiumUniRule annotation1 Publication1
Active sitei217UniRule annotation1 Publication1
Metal bindingi217MagnesiumUniRule annotation1 Publication1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: EcoCyc

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processStress response
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11831-MONOMER
MetaCyc:EG11831-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Stress response kinase A1 PublicationUniRule annotation (EC:2.7.11.1UniRule annotation1 Publication)
Alternative name(s):
Serine/threonine protein kinase YihE1 Publication
Serine/threonine-protein kinase SrkAUniRule annotationCurated
Gene namesi
Name:srkA1 PublicationUniRule annotation
Synonyms:rdoA, yihE
Ordered Locus Names:b3859, JW3831
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11831 srkA

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

A hyperlethal phenotype (reduced survival in the presence of antibiotic but no change in minimal inhibitory concentration) for a number of antimicrobials including nalidixic acid, tetracycline, ampicillin and mitomycin C as well as exposure to UV light and H2O2. Lethality is mitigated by pretreatment with 2,2'-bipyridyl and thiourea, which inhibit hydroxyl radical accumulation. No change in cell survival upon heat shock (up to 55 degrees Celsius), rifampicin or fluoroquinolone PD161144 treatment. A triple srkA-mazE-mazF disruption mutant shows no hyperlethality in the presence of nalidixic acid or UV light, suggesting SrkA has a negative effect on MazF. Double katG-srkA and double cpxR-srkA disruption mutants are as sensitive to killing as single srkA mutants; i.e. all 3 genes are epistatic and function in the same genetic pathway.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi36S → A: Partial loss of kinase activity, only partially protects bacteria against hyperlethal stress. 1 Publication1
Mutagenesisi217D → A: Loss of kinase activity; does not protect bacteria against hyperlethal stress. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002095741 – 328Stress response kinase AAdd BLAST328

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0C0K3
PRIDEiP0C0K3

Expressioni

Inductioni

Induced by the two-component Cpx system that responds to extracellular stress.1 Publication

Interactioni

Subunit structurei

Monomer.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4261338, 3 interactors
IntActiP0C0K3, 5 interactors
STRINGi316385.ECDH10B_4048

Structurei

Secondary structure

1328
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi13 – 22Combined sources10
Beta strandi32 – 34Combined sources3
Beta strandi37 – 44Combined sources8
Beta strandi52 – 57Combined sources6
Turni59 – 61Combined sources3
Helixi64 – 79Combined sources16
Beta strandi96 – 98Combined sources3
Beta strandi101 – 107Combined sources7
Helixi119 – 136Combined sources18
Beta strandi142 – 144Combined sources3
Helixi149 – 152Combined sources4
Helixi154 – 161Combined sources8
Beta strandi164 – 166Combined sources3
Turni168 – 170Combined sources3
Helixi171 – 188Combined sources18
Helixi204 – 206Combined sources3
Beta strandi207 – 215Combined sources9
Helixi228 – 231Combined sources4
Helixi238 – 252Combined sources15
Turni253 – 255Combined sources3
Helixi260 – 265Combined sources6
Helixi266 – 283Combined sources18
Turni284 – 286Combined sources3
Helixi289 – 293Combined sources5
Helixi295 – 298Combined sources4
Helixi300 – 316Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZYLX-ray2.80A1-328[»]
ProteinModelPortaliP0C0K3
SMRiP0C0K3
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0C0K3

Family & Domainsi

Sequence similaritiesi

Belongs to the SrkA/RdoA protein kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E27 Bacteria
COG2334 LUCA
HOGENOMiHOG000265894
InParanoidiP0C0K3
OMAiLIHYSAW
PhylomeDBiP0C0K3

Family and domain databases

HAMAPiMF_01497 SrkA_kinase, 1 hit
InterProiView protein in InterPro
IPR002575 Aminoglycoside_PTrfase
IPR011009 Kinase-like_dom_sf
IPR032882 SrkA/RdoA
PANTHERiPTHR39573 PTHR39573, 1 hit
PfamiView protein in Pfam
PF01636 APH, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit

Sequencei

Sequence statusi: Complete.

P0C0K3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNSAFTFQT LHPDTIMDAL FEHGIRVDSG LTPLNSYENR VYQFQDEDRR
60 70 80 90 100
RFVVKFYRPE RWTADQILEE HQFALQLVND EVPVAAPVAF NGQTLLNHQG
110 120 130 140 150
FYFAVFPSVG GRQFEADNID QMEAVGRYLG RMHQTGRKQL FIHRPTIGLN
160 170 180 190 200
EYLIEPRKLF EDATLIPSGL KAAFLKATDE LIAAVTAHWR EDFTVLRLHG
210 220 230 240 250
DCHAGNILWR DGPMFVDLDD ARNGPAVQDL WMLLNGDKAE QRMQLETIIE
260 270 280 290 300
AYEEFSEFDT AEIGLIEPLR AMRLVYYLAW LMRRWADPAF PKNFPWLTGE
310 320
DYWLRQTATF IEQAKVLQEP PLQLTPMY
Length:328
Mass (Da):38,120
Last modified:September 27, 2005 - v1
Checksum:i100AEEF0FBF04436
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80762 Genomic DNA Translation: CAA56735.1
L19201 Genomic DNA Translation: AAB02994.1
U00096 Genomic DNA Translation: AAC76857.1
AP009048 Genomic DNA Translation: BAE77449.1
PIRiS40805
RefSeqiNP_418296.1, NC_000913.3
WP_001065497.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76857; AAC76857; b3859
BAE77449; BAE77449; BAE77449
GeneIDi948346
KEGGiecj:JW3831
eco:b3859
PATRICifig|511145.12.peg.3968

Similar proteinsi

Entry informationi

Entry nameiSRKA_ECOLI
AccessioniPrimary (citable) accession number: P0C0K3
Secondary accession number(s): P32127, Q2M8F7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: September 27, 2005
Last modified: March 28, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health