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Protein

Streptopain

Gene

speB

Organism
Streptococcus pyogenes serotype M1
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Important streptococcal virulence factor which cleaves human fibronectin and degrades vitronectin. Also cleaves human IL1B precursor to form biologically active IL1B. Can induce apoptosis in human monocytes and epithelial cells in vitro, and reduces phagocytic activity in monocytic cells. Thus, may play a role in bacterial colonization, invasion, and inhibition of wound healing.1 Publication

Catalytic activityi

Preferential cleavage with hydrophobic residues at P2, P1 and P1'.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei192NucleophileBy similarity1
Active sitei340Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease, Toxin

Keywords - Biological processi

Virulence

Enzyme and pathway databases

BRENDAi3.4.22.10. 5935.

Names & Taxonomyi

Protein namesi
Recommended name:
Streptopain (EC:3.4.22.10)
Alternative name(s):
Exotoxin type B
SPE B
Streptococcal cysteine proteinase
Streptococcus peptidase A
Short name:
SPP
Gene namesi
Name:speB
Ordered Locus Names:SPy_2039, M5005_Spy1735
OrganismiStreptococcus pyogenes serotype M1
Taxonomic identifieri301447 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000750 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi334V → A: Reduces activity 130-fold. 1 Publication1
Mutagenesisi357W → A: Reduces activity 280-fold. 1 Publication1
Mutagenesisi359W → A: Reduces activity 420-fold. 1 Publication1
Mutagenesisi384G → D: Reduces activity 14-fold. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27By similarityAdd BLAST27
PropeptideiPRO_000004193928 – 145By similarityAdd BLAST118
ChainiPRO_0000041940146 – 398StreptopainAdd BLAST253

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei192Cysteine methyl disulfide; in zymogen formBy similarity1

Keywords - PTMi

Methylation, Zymogen

Proteomic databases

PaxDbiP0C0J1.
PRIDEiP0C0J1.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi160490.SPy_2039.

Structurei

Secondary structure

1398
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi152 – 155Combined sources4
Beta strandi162 – 164Combined sources3
Helixi165 – 167Combined sources3
Beta strandi168 – 170Combined sources3
Helixi192 – 204Combined sources13
Beta strandi214 – 217Combined sources4
Beta strandi230 – 233Combined sources4
Helixi235 – 237Combined sources3
Turni242 – 244Combined sources3
Helixi255 – 271Combined sources17
Beta strandi277 – 279Combined sources3
Helixi285 – 294Combined sources10
Beta strandi303 – 305Combined sources3
Helixi307 – 309Combined sources3
Helixi312 – 324Combined sources13
Beta strandi329 – 334Combined sources6
Beta strandi339 – 351Combined sources13
Beta strandi353 – 356Combined sources4
Beta strandi358 – 363Combined sources6
Beta strandi388 – 394Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JTCNMR-A146-398[»]
ProteinModelPortaliP0C0J1.
SMRiP0C0J1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0C0J1.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C10 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105JBQ. Bacteria.
ENOG4111S8V. LUCA.
KOiK01364.
OMAiWESQIDK.

Family and domain databases

InterProiIPR000200. Peptidase_C10.
IPR025896. Spi_Prtas-inh.
[Graphical view]
PfamiPF13734. Inhibitor_I69. 1 hit.
PF01640. Peptidase_C10. 1 hit.
[Graphical view]
PRINTSiPR00797. STREPTOPAIN.
ProDomiPD004169. Peptidase_C10. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C0J1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKKKLGVRL LSLLALGGFV LANPVFADQN FARNEKEAKD SAITFIQKSA
60 70 80 90 100
AIKAGARSAE DIKLDKVNLG GELSGSNMYV YNISTGGFVI VSGDKRSPEI
110 120 130 140 150
LGYSTSGSFD ANGKENIASF MESYVEQIKE NKKLDTTYAG TAEIKQPVVK
160 170 180 190 200
SLLDSKGIHY NQGNPYNLLT PVIEKVKPGE QSFVGQHAAT GCVATATAQI
210 220 230 240 250
MKYHNYPNKG LKDYTYTLSS NNPYFNHPKN LFAAISTRQY NWNNILPTYS
260 270 280 290 300
GRESNVQKMA ISELMADVGI SVDMDYGPSS GSAGSSRVQR ALKENFGYNQ
310 320 330 340 350
SVHQINRGDF SKQDWEAQID KELSQNQPVY YQGVGKVGGH AFVIDGADGR
360 370 380 390
NFYHVNWGWG GVSDGFFRLD ALNPSALGTG GGAGGFNGYQ SAVVGIKP
Length:398
Mass (Da):43,130
Last modified:September 13, 2005 - v1
Checksum:i4D60DB0B80D687B4
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti8V → I in strain: 789. 1
Natural varianti193V → L in strain: A-20. 1
Natural varianti308G → S in strain: 789. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26158 Genomic DNA. Translation: AAA26987.1.
AF104940 Genomic DNA. Translation: AAD17930.1.
AE004092 Genomic DNA. Translation: AAK34706.1.
CP000017 Genomic DNA. Translation: AAZ52353.1.
RefSeqiNP_269985.1. NC_002737.2.

Genome annotation databases

EnsemblBacteriaiAAK34706; AAK34706; SPy_2039.
AAZ52353; AAZ52353; M5005_Spy1735.
GeneIDi901690.
KEGGispy:SPy_2039.
spz:M5005_Spy1735.
PATRICi19717447. VBIStrPyo79812_1768.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26158 Genomic DNA. Translation: AAA26987.1.
AF104940 Genomic DNA. Translation: AAD17930.1.
AE004092 Genomic DNA. Translation: AAK34706.1.
CP000017 Genomic DNA. Translation: AAZ52353.1.
RefSeqiNP_269985.1. NC_002737.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JTCNMR-A146-398[»]
ProteinModelPortaliP0C0J1.
SMRiP0C0J1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi160490.SPy_2039.

Proteomic databases

PaxDbiP0C0J1.
PRIDEiP0C0J1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK34706; AAK34706; SPy_2039.
AAZ52353; AAZ52353; M5005_Spy1735.
GeneIDi901690.
KEGGispy:SPy_2039.
spz:M5005_Spy1735.
PATRICi19717447. VBIStrPyo79812_1768.

Phylogenomic databases

eggNOGiENOG4105JBQ. Bacteria.
ENOG4111S8V. LUCA.
KOiK01364.
OMAiWESQIDK.

Enzyme and pathway databases

BRENDAi3.4.22.10. 5935.

Miscellaneous databases

EvolutionaryTraceiP0C0J1.

Family and domain databases

InterProiIPR000200. Peptidase_C10.
IPR025896. Spi_Prtas-inh.
[Graphical view]
PfamiPF13734. Inhibitor_I69. 1 hit.
PF01640. Peptidase_C10. 1 hit.
[Graphical view]
PRINTSiPR00797. STREPTOPAIN.
ProDomiPD004169. Peptidase_C10. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Entry informationi

Entry nameiSPEB_STRP1
AccessioniPrimary (citable) accession number: P0C0J1
Secondary accession number(s): P00788
, P26296, P68883, Q48WC2, Q54960, Q54961, Q54962, Q54963, Q54964, Q54965, Q54966, Q54967, Q54968, Q57024, Q57082, Q57202, Q57211, Q57212, Q9S680
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: September 13, 2005
Last modified: November 2, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.