Reviewed, UniProtKB/Swiss-Prot P0C0J1 (SPEB_STRP1)

Names and origin

Protein names

Recommended name:
    Streptopain
    EC=3.4.22.10
Alternative name(s):
    Streptococcal cysteine proteinase
    Streptococcus peptidase A
      Short name=SPP
    Exotoxin type B
    SPE B

Gene names

Name:	speB
Ordered Locus Names:	SPy_2039, M5005_Spy1735

Organism

Streptococcus pyogenes serotype M1 [Complete proteome] [HAMAP]

Taxonomic identifier

301447 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Important streptococcal virulence factor which cleaves human fibronectin and degrades vitronectin. Also cleaves human IL1B precursor to form biologically active IL1B. Can induce apoptosis in human monocytes and epithelial cells in vitro, and reduces phagocytic activity in monocytic cells. Thus, may play a role in bacterial colonization, invasion, and inhibition of wound healing. Ref.5
Catalytic activity	Preferential cleavage with hydrophobic residues at P2, P1 and P1'.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase C10 family.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease Toxin
PTM	Zymogen
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	By similarity
	Propeptide	28 – 145	118	By similarity		PRO_0000041939
	Chain	146 – 398	253	Streptopain		PRO_0000041940
Sites
	Active site	192	1	By similarity
	Active site	340	1	By similarity
Amino acid modifications
	Modified residue	192	1	Cysteine methyl disulfide; in zymogen form By similarity
Natural variations
	Natural variant	8	1	V → I in strain: 789.
	Natural variant	193	1	V → L in strain: A-20.
	Natural variant	308	1	G → S in strain: 789.

Sequences

	Sequence		Length	Mass (Da)
	P0C0J1-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 4D60DB0B80D687B4	FASTA	398	43,130
	10 20 30 40 50 60 MNKKKLGVRL LSLLALGGFV LANPVFADQN FARNEKEAKD SAITFIQKSA AIKAGARSAE 70 80 90 100 110 120 DIKLDKVNLG GELSGSNMYV YNISTGGFVI VSGDKRSPEI LGYSTSGSFD ANGKENIASF 130 140 150 160 170 180 MESYVEQIKE NKKLDTTYAG TAEIKQPVVK SLLDSKGIHY NQGNPYNLLT PVIEKVKPGE 190 200 210 220 230 240 QSFVGQHAAT GCVATATAQI MKYHNYPNKG LKDYTYTLSS NNPYFNHPKN LFAAISTRQY 250 260 270 280 290 300 NWNNILPTYS GRESNVQKMA ISELMADVGI SVDMDYGPSS GSAGSSRVQR ALKENFGYNQ 310 320 330 340 350 360 SVHQINRGDF SKQDWEAQID KELSQNQPVY YQGVGKVGGH AFVIDGADGR NFYHVNWGWG 370 380 390 GVSDGFFRLD ALNPSALGTG GGAGGFNGYQ SAVVGIKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A conserved Streptococcus pyogenes extracellular cysteine protease cleaves human fibronectin and degrades vitronectin." Kapur V., Topouzis S., Majesky M.W., Li L.L., Hamrick M.R., Hamill R.J., Patti J.M., Musser J.M. Microb. Pathog. 15:327-346(1993) [PubMed: 7516997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: 789 / Serotype M1.
[2]	Wu J.-J. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A-20 / Serotype M1,T1.
[3]	"Complete genome sequence of an M1 strain of Streptococcus pyogenes." Ferretti J.J., McShan W.M., Ajdic D.J., Savic D.J., Savic G., Lyon K., Primeaux C., Sezate S., Suvorov A.N., Kenton S., Lai H.S., Lin S.P., Qian Y., Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L. , White J., Yuan X., Clifton S.W., Roe B.A., McLaughlin R.E. Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001) [PubMed: 11296296] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700294 / SF370 / Serotype M1.
[4]	"Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events." Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M. J. Infect. Dis. 192:771-782(2005) [PubMed: 16088826] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.
[5]	"Group A Streptococcus induces apoptosis in human epithelial cells." Tsai P.-J., Lin Y.-S., Kuo C.-F., Lei H.-Y., Wu J.-J. Infect. Immun. 67:4334-4339(1999) [PubMed: 10456871] [Abstract] Cited for: FUNCTION. Strain: A-20 / Serotype M1,T1.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L26158 Genomic DNA. Translation: AAA26987.1.
AF104940 Genomic DNA. Translation: AAD17930.1.
AE004092 Genomic DNA. Translation: AAK34706.1.
CP000017 Genomic DNA. Translation: AAZ52353.1.

RefSeq

NP_269985.1.
YP_283098.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2JTC	NMR	-	A	146-398	[»]

SMR

P0C0J1. Positions 31-398.

ModBase

Search...

Genome annotation databases

GeneID

3571136.
901690.

GenomeReviews

Gene locus SPy_2039 in contig AE004092_GR.
Gene locus M5005_Spy1735 in contig CP000017_GR.

KEGG

spy:SPy_2039.
spz:M5005_Spy_1735.

Organism-specific databases

CMR

Search...

Phylogenomic databases

OMA

P0C0J1. WESQIDK.

Enzyme and pathway databases

BioCyc

SPYO160490:SPY2039-MON.
SPYO293653:M5005_SPY1735-MON.

Family and domain databases

InterPro

IPR000200. Peptidase_C10.
[Graphical view]

Pfam

PF01640. Peptidase_C10. 1 hit.
[Graphical view]

PRINTS

PR00797. STREPTOPAIN.

ProDom

PD004169. Peptidase_C10. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Entry information

Entry name

SPEB_STRP1

Accession

Primary (citable) accession number: P0C0J1
Secondary accession number(s): P00788 , P26296, P68883, Q48WC2, Q54960, Q54961, Q54962, Q54963, Q54964, Q54965, Q54966, Q54967, Q54968, Q57024, Q57082, Q57202, Q57211, Q57212, Q9S680

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)