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Protein

Streptopain

Gene

speB

Organism
Streptococcus pyogenes
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Important streptococcal virulence factor which cleaves human fibronectin and degrades vitronectin. Also cleaves human IL1B precursor to form biologically active IL1B. Can induce apoptosis in human monocytes and epithelial cells in vitro, and reduces phagocytic activity in monocytic cells. Thus, may play a role in bacterial colonization, invasion, and inhibition of wound healing.1 Publication

Catalytic activityi

Preferential cleavage with hydrophobic residues at P2, P1 and P1'.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei192Nucleophile1 Publication1
Active sitei340Proton acceptor1 Publication1

GO - Molecular functioni

GO - Biological processi

  • pathogenesis Source: UniProtKB-KW
  • proteolysis in other organism Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease, Toxin

Keywords - Biological processi

Virulence

Enzyme and pathway databases

BRENDAi3.4.22.10. 5935.
SABIO-RKP0C0J0.

Names & Taxonomyi

Protein namesi
Recommended name:
Streptopain (EC:3.4.22.10)
Alternative name(s):
Exotoxin type B
SPE B
Streptococcal cysteine proteinase
Streptococcus peptidase A
Short name:
SPP
Gene namesi
Name:speB
OrganismiStreptococcus pyogenes
Taxonomic identifieri1314 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2034806.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 271 PublicationAdd BLAST27
PropeptideiPRO_000002850328 – 1451 PublicationAdd BLAST118
ChainiPRO_0000028504146 – 398StreptopainAdd BLAST253

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei192Cysteine methyl disulfide; in zymogen form1 Publication1

Keywords - PTMi

Methylation, Zymogen

Proteomic databases

PaxDbiP0C0J0.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi160490.SPy_2039.

Chemistry databases

BindingDBiP0C0J0.

Structurei

Secondary structure

1398
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi35 – 38Combined sources4
Helixi40 – 47Combined sources8
Beta strandi64 – 66Combined sources3
Turni71 – 73Combined sources3
Beta strandi75 – 82Combined sources8
Beta strandi84 – 86Combined sources3
Beta strandi88 – 93Combined sources6
Beta strandi99 – 107Combined sources9
Helixi115 – 130Combined sources16
Helixi131 – 133Combined sources3
Helixi152 – 155Combined sources4
Turni164 – 169Combined sources6
Helixi192 – 204Combined sources13
Beta strandi214 – 217Combined sources4
Beta strandi230 – 233Combined sources4
Helixi235 – 237Combined sources3
Turni242 – 244Combined sources3
Helixi255 – 271Combined sources17
Beta strandi277 – 279Combined sources3
Helixi285 – 294Combined sources10
Beta strandi303 – 306Combined sources4
Helixi307 – 309Combined sources3
Helixi312 – 324Combined sources13
Beta strandi329 – 335Combined sources7
Beta strandi338 – 351Combined sources14
Beta strandi353 – 356Combined sources4
Turni360 – 363Combined sources4
Beta strandi365 – 368Combined sources4
Helixi375 – 377Combined sources3
Beta strandi388 – 394Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DKIX-ray1.60A/B/C/D28-398[»]
1PVJX-ray3.00A/B/C/D31-398[»]
2UZJX-ray1.55A/B146-398[»]
ProteinModelPortaliP0C0J0.
SMRiP0C0J0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0C0J0.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C10 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105JBQ. Bacteria.
ENOG4111S8V. LUCA.

Family and domain databases

InterProiIPR000200. Peptidase_C10.
IPR025896. Spi_Prtas-inh.
[Graphical view]
PfamiPF13734. Inhibitor_I69. 1 hit.
PF01640. Peptidase_C10. 1 hit.
[Graphical view]
PRINTSiPR00797. STREPTOPAIN.
ProDomiPD004169. Peptidase_C10. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C0J0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKKKLGIRL LSLLALGGFV LANPVFADQN FARNEKEAKD SAITFIQKSA
60 70 80 90 100
AIKAGARSAE DIKLDKVNLG GELSGSNMYV YNISTGGFVI VSGDKRSPEI
110 120 130 140 150
LGYSTSGSFD ANGKENIASF MESYVEQIKE NKKLDTTYAG TAEIKQPVVK
160 170 180 190 200
SLLDSKGIHY NQGNPYNLLT PVIEKVKPGE QSFVGQHAAT GCVATATAQI
210 220 230 240 250
MKYHNYPNKG LKDYTYTLSS NNPYFNHPKN LFAAISTRQY NWNNILPTYS
260 270 280 290 300
GRESNVQKMA ISELMADVGI SVDMDYGPSS GSAGSSRVQR ALKENFGYNQ
310 320 330 340 350
SVHQINRSDF SKQDWEAQID KELSQNQPVY YQGVGKVGGH AFVIDGADGR
360 370 380 390
NFYHVNWGWG GVSDGFFRLD ALNPSALGTG GGAGGFNGYQ SAVVGIKP
Length:398
Mass (Da):43,174
Last modified:September 13, 2005 - v1
Checksum:i16FF180D720AEE0F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti84 – 85ST → AS AA sequence (Ref. 4) Curated2
Sequence conflicti169L → I AA sequence (Ref. 4) Curated1
Sequence conflicti187 – 191HAATG → AATGH AA sequence (Ref. 4) Curated5
Sequence conflicti187 – 191HAATG → AATGH AA sequence (PubMed:1270417).Curated5
Sequence conflicti208N → D AA sequence (Ref. 4) Curated1
Sequence conflicti208N → D AA sequence (PubMed:1270417).Curated1
Sequence conflicti213D → N AA sequence (Ref. 4) Curated1
Sequence conflicti213D → N AA sequence (PubMed:1270417).Curated1
Sequence conflicti222 – 223NP → PD AA sequence (Ref. 4) Curated2
Sequence conflicti222 – 223NP → PD AA sequence (PubMed:1270417).Curated2
Sequence conflicti226N → D AA sequence (Ref. 4) Curated1
Sequence conflicti226N → D AA sequence (PubMed:1270417).Curated1
Sequence conflicti241N → D AA sequence (Ref. 4) Curated1
Sequence conflicti241N → D AA sequence (PubMed:1270417).Curated1
Sequence conflicti253 – 257ESNVQ → QSQNV AA sequence (Ref. 4) Curated5
Sequence conflicti253 – 257ESNVQ → QSQNV AA sequence (PubMed:1270417).Curated5
Sequence conflicti306N → D AA sequence (Ref. 4) Curated1
Sequence conflicti332Q → E AA sequence (Ref. 4) Curated1
Sequence conflicti332Q → E AA sequence (PubMed:1270417).Curated1
Sequence conflicti346 – 348GAD → DGA AA sequence (Ref. 4) Curated3
Sequence conflicti346 – 348GAD → DGA AA sequence (PubMed:1270417).Curated3
Sequence conflicti356N → D AA sequence (Ref. 4) Curated1
Sequence conflicti356N → D AA sequence (PubMed:1270417).Curated1
Sequence conflicti390Q → E AA sequence (Ref. 4) Curated1
Sequence conflicti390Q → E AA sequence (PubMed:1270417).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti17G → S in strain: MGAS 1896. 1
Natural varianti80V → I in strain: MGAS 168. 1
Natural varianti111A → V in strain: MGAS 165, 168, 429, 659, 660, 796, 800, 1719, 1838, 1882, 2017 and 2018. 1
Natural varianti137T → I in strain: MGAS 650. 1
Natural varianti154D → N in strain: MGAS 684. 1
Natural varianti211L → V in strain: MGAS 366, 427, 758, 1294, 1911, 1914A and 1991. 1
Natural varianti305I → V in strain: MGAS 1901 and Sv. 1
Natural varianti308S → G in strain: MGAS 429, 659, 807, 1226, 1719, 1832, 1842, 1871, 1872, 2017 and 2018. 1
Natural varianti317A → S in strain: MGAS 165, 168, 289, 302, 1233 and 1898. 1
Natural varianti384G → D in strain: MGAS 1871. 1
Natural varianti394V → I in strain: MGAS 366 and 1294. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86905 Genomic DNA. Translation: AAA26978.1.
L26126 Genomic DNA. Translation: AAA26992.1.
L26127 Genomic DNA. Translation: AAA26993.1.
L26128 Genomic DNA. Translation: AAA26994.1.
L26129 Genomic DNA. Translation: AAA26995.1.
L26130 Genomic DNA. Translation: AAA26996.1.
L26131 Genomic DNA. Translation: AAA26997.1.
L26132 Genomic DNA. Translation: AAA26998.1.
L26133 Genomic DNA. Translation: AAA26999.1.
L26135 Genomic DNA. Translation: AAA27001.1.
L26136 Genomic DNA. Translation: AAA27002.1.
L26137 Genomic DNA. Translation: AAA27003.1.
L26138 Genomic DNA. Translation: AAA27004.1.
L26139 Genomic DNA. Translation: AAA27005.1.
L26140 Genomic DNA. Translation: AAA27006.1.
L26141 Genomic DNA. Translation: AAA27007.1.
L26142 Genomic DNA. Translation: AAA27008.1.
L26143 Genomic DNA. Translation: AAA27009.1.
L26144 Genomic DNA. Translation: AAA27010.1.
L26145 Genomic DNA. Translation: AAA27011.1.
L26147 Genomic DNA. Translation: AAA27013.1.
L26148 Genomic DNA. Translation: AAA27014.1.
L26149 Genomic DNA. Translation: AAA27015.1.
L26150 Genomic DNA. Translation: AAA27016.1.
L26151 Genomic DNA. Translation: AAA26980.1.
L26152 Genomic DNA. Translation: AAA26981.1.
L26153 Genomic DNA. Translation: AAA26982.1.
L26154 Genomic DNA. Translation: AAA26983.1.
L26155 Genomic DNA. Translation: AAA26984.1.
L26156 Genomic DNA. Translation: AAA26985.1.
L26157 Genomic DNA. Translation: AAA26986.1.
L26159 Genomic DNA. Translation: AAA26988.1.
L26160 Genomic DNA. Translation: AAA26989.1.
L26161 Genomic DNA. Translation: AAA26990.1.
L26162 Genomic DNA. Translation: AAA26991.1.
AB030578 Genomic DNA. Translation: BAB16027.1.
PIRiA37768.
RefSeqiWP_002991253.1. NZ_LVYT01000012.1.
WP_009881074.1. NZ_CP010449.1.
WP_011285235.1. NZ_CP014139.1.
WP_014407896.1. NZ_LRHJ01000010.1.
WP_021733538.1. NZ_LRHD01000012.1.
WP_023611203.1. NZ_CP014278.2.
WP_031488619.1. NZ_LVYS01000018.1.
WP_038433740.1. NZ_CP008695.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86905 Genomic DNA. Translation: AAA26978.1.
L26126 Genomic DNA. Translation: AAA26992.1.
L26127 Genomic DNA. Translation: AAA26993.1.
L26128 Genomic DNA. Translation: AAA26994.1.
L26129 Genomic DNA. Translation: AAA26995.1.
L26130 Genomic DNA. Translation: AAA26996.1.
L26131 Genomic DNA. Translation: AAA26997.1.
L26132 Genomic DNA. Translation: AAA26998.1.
L26133 Genomic DNA. Translation: AAA26999.1.
L26135 Genomic DNA. Translation: AAA27001.1.
L26136 Genomic DNA. Translation: AAA27002.1.
L26137 Genomic DNA. Translation: AAA27003.1.
L26138 Genomic DNA. Translation: AAA27004.1.
L26139 Genomic DNA. Translation: AAA27005.1.
L26140 Genomic DNA. Translation: AAA27006.1.
L26141 Genomic DNA. Translation: AAA27007.1.
L26142 Genomic DNA. Translation: AAA27008.1.
L26143 Genomic DNA. Translation: AAA27009.1.
L26144 Genomic DNA. Translation: AAA27010.1.
L26145 Genomic DNA. Translation: AAA27011.1.
L26147 Genomic DNA. Translation: AAA27013.1.
L26148 Genomic DNA. Translation: AAA27014.1.
L26149 Genomic DNA. Translation: AAA27015.1.
L26150 Genomic DNA. Translation: AAA27016.1.
L26151 Genomic DNA. Translation: AAA26980.1.
L26152 Genomic DNA. Translation: AAA26981.1.
L26153 Genomic DNA. Translation: AAA26982.1.
L26154 Genomic DNA. Translation: AAA26983.1.
L26155 Genomic DNA. Translation: AAA26984.1.
L26156 Genomic DNA. Translation: AAA26985.1.
L26157 Genomic DNA. Translation: AAA26986.1.
L26159 Genomic DNA. Translation: AAA26988.1.
L26160 Genomic DNA. Translation: AAA26989.1.
L26161 Genomic DNA. Translation: AAA26990.1.
L26162 Genomic DNA. Translation: AAA26991.1.
AB030578 Genomic DNA. Translation: BAB16027.1.
PIRiA37768.
RefSeqiWP_002991253.1. NZ_LVYT01000012.1.
WP_009881074.1. NZ_CP010449.1.
WP_011285235.1. NZ_CP014139.1.
WP_014407896.1. NZ_LRHJ01000010.1.
WP_021733538.1. NZ_LRHD01000012.1.
WP_023611203.1. NZ_CP014278.2.
WP_031488619.1. NZ_LVYS01000018.1.
WP_038433740.1. NZ_CP008695.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DKIX-ray1.60A/B/C/D28-398[»]
1PVJX-ray3.00A/B/C/D31-398[»]
2UZJX-ray1.55A/B146-398[»]
ProteinModelPortaliP0C0J0.
SMRiP0C0J0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi160490.SPy_2039.

Chemistry databases

BindingDBiP0C0J0.
ChEMBLiCHEMBL2034806.

Proteomic databases

PaxDbiP0C0J0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105JBQ. Bacteria.
ENOG4111S8V. LUCA.

Enzyme and pathway databases

BRENDAi3.4.22.10. 5935.
SABIO-RKP0C0J0.

Miscellaneous databases

EvolutionaryTraceiP0C0J0.

Family and domain databases

InterProiIPR000200. Peptidase_C10.
IPR025896. Spi_Prtas-inh.
[Graphical view]
PfamiPF13734. Inhibitor_I69. 1 hit.
PF01640. Peptidase_C10. 1 hit.
[Graphical view]
PRINTSiPR00797. STREPTOPAIN.
ProDomiPD004169. Peptidase_C10. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Entry informationi

Entry nameiSPEB_STRPY
AccessioniPrimary (citable) accession number: P0C0J0
Secondary accession number(s): P00788
, P26296, P68883, Q54960, Q54961, Q54962, Q54963, Q54964, Q54965, Q54966, Q54967, Q54968, Q57024, Q57082, Q57202, Q57211, Q57212, Q9S680
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: September 13, 2005
Last modified: November 2, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.