Reviewed, UniProtKB/Swiss-Prot P0C0J0 (SPEB_STRPY)

Names and origin

Protein names

Recommended name:
    Streptopain
    EC=3.4.22.10
Alternative name(s):
    Streptococcal cysteine proteinase
    Streptococcus peptidase A
      Short name=SPP
    Exotoxin type B
    SPE B

Gene names

Name:

speB

Organism

Streptococcus pyogenes

Taxonomic identifier

1314 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Important streptococcal virulence factor which cleaves human fibronectin and degrades vitronectin. Also cleaves human IL1B precursor to form biologically active IL1B. Can induce apoptosis in human monocytes and epithelial cells in vitro, and reduces phagocytic activity in monocytic cells. Thus, may play a role in bacterial colonization, invasion, and inhibition of wound healing. Ref.7
Catalytic activity	Preferential cleavage with hydrophobic residues at P2, P1 and P1'.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase C10 family.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease Toxin
PTM	Methylation Zymogen
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 27

27

Ref.1 Ref.4

Propeptide

28 – 145

118

Ref.5

PRO_0000028503

Chain

146 – 398

253

Streptopain

PRO_0000028504

Sites

Active site

192

1

Active site

340

1

Amino acid modifications

Modified residue

192

1

Cysteine methyl disulfide; in zymogen form

Natural variations

Natural variant

17

1

G → S in strain: MGAS 1896.

Natural variant

80

1

V → I in strain: MGAS 168.

Natural variant

111

1

A → V in strain: MGAS 165, 168, 429, 659, 660, 796, 800, 1719, 1838, 1882, 2017 and 2018.

Natural variant

137

1

T → I in strain: MGAS 650.

Natural variant

154

1

D → N in strain: MGAS 684.

Natural variant

211

1

L → V in strain: MGAS 366, 427, 758, 1294, 1911, 1914A and 1991.

Natural variant

305

1

I → V in strain: MGAS 1901 and Sv.

Natural variant

308

1

S → G in strain: MGAS 429, 659, 807, 1226, 1719, 1832, 1842, 1871, 1872, 2017 and 2018.

Natural variant

317

1

A → S in strain: MGAS 165, 168, 289, 302, 1233 and 1898.

Natural variant

384

1

G → D in strain: MGAS 1871.

Natural variant

394

1

V → I in strain: MGAS 366 and 1294.

Experimental info

Sequence conflict

84 – 85

2

ST → AS AA sequence Ref.4

Sequence conflict

169

1

L → I AA sequence Ref.4

Sequence conflict

187 – 191

5

HAATG → AATGH AA sequence Ref.4

Sequence conflict

187 – 191

5

HAATG → AATGH AA sequence Ref.5

Sequence conflict

208

1

N → D AA sequence Ref.4

Sequence conflict

208

1

N → D AA sequence Ref.5

Sequence conflict

213

1

D → N AA sequence Ref.4

Sequence conflict

213

1

D → N AA sequence Ref.5

Sequence conflict

222 – 223

2

NP → PD AA sequence Ref.4

Sequence conflict

222 – 223

2

NP → PD AA sequence Ref.5

Sequence conflict

226

1

N → D AA sequence Ref.4

Sequence conflict

226

1

N → D AA sequence Ref.5

Sequence conflict

241

1

N → D AA sequence Ref.4

Sequence conflict

241

1

N → D AA sequence Ref.5

Sequence conflict

253 – 257

5

ESNVQ → QSQNV Ref.4

Sequence conflict

253 – 257

5

ESNVQ → QSQNV AA sequence Ref.5

Sequence conflict

306

1

N → D AA sequence; AA sequence Ref.4

Sequence conflict

332

1

Q → E AA sequence Ref.4

Sequence conflict

332

1

Q → E AA sequence Ref.5

Sequence conflict

346 – 348

3

GAD → DGA AA sequence Ref.4

Sequence conflict

346 – 348

3

GAD → DGA AA sequence Ref.5

Sequence conflict

356

1

N → D AA sequence Ref.4

Sequence conflict

356

1

N → D AA sequence Ref.5

Sequence conflict

390

1

Q → E AA sequence Ref.4

Sequence conflict

390

1

Q → E AA sequence Ref.5

Secondary structure

1

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398

Helix Strand Turn

Details...

Helix

35 – 47

13

Beta strand

64 – 66

3

Turn

71 – 73

3

Beta strand

75 – 83

9

Beta strand

88 – 94

7

Beta strand

99 – 107

9

Helix

115 – 128

14

Helix

130 – 133

4

Helix

154 – 156

3

Helix

166 – 169

4

Helix

192 – 204

13

Beta strand

214 – 217

4

Beta strand

230 – 233

4

Helix

235 – 237

3

Turn

242 – 244

3

Helix

255 – 271

17

Beta strand

277 – 279

3

Helix

285 – 294

10

Beta strand

303 – 306

4

Helix

307 – 309

3

Helix

312 – 324

13

Beta strand

329 – 332

4

Beta strand

342 – 347

6

Beta strand

349 – 351

3

Beta strand

353 – 356

4

Turn

360 – 363

4

Beta strand

365 – 367

3

Beta strand

391 – 394

4

Sequences

	Sequence		Length	Mass (Da)
	P0C0J0-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: 16FF180D720AEE0F	FASTA	398	43,174
	10 20 30 40 50 60 MNKKKLGIRL LSLLALGGFV LANPVFADQN FARNEKEAKD SAITFIQKSA AIKAGARSAE 70 80 90 100 110 120 DIKLDKVNLG GELSGSNMYV YNISTGGFVI VSGDKRSPEI LGYSTSGSFD ANGKENIASF 130 140 150 160 170 180 MESYVEQIKE NKKLDTTYAG TAEIKQPVVK SLLDSKGIHY NQGNPYNLLT PVIEKVKPGE 190 200 210 220 230 240 QSFVGQHAAT GCVATATAQI MKYHNYPNKG LKDYTYTLSS NNPYFNHPKN LFAAISTRQY 250 260 270 280 290 300 NWNNILPTYS GRESNVQKMA ISELMADVGI SVDMDYGPSS GSAGSSRVQR ALKENFGYNQ 310 320 330 340 350 360 SVHQINRSDF SKQDWEAQID KELSQNQPVY YQGVGKVGGH AFVIDGADGR NFYHVNWGWG 370 380 390 GVSDGFFRLD ALNPSALGTG GGAGGFNGYQ SAVVGIKP

References

[1]	"Nucleotide sequence of the streptococcal pyrogenic exotoxin type B gene and relationship between the toxin and the streptococcal proteinase precursor." Hauser A.R., Schlievert P.M. J. Bacteriol. 172:4536-4542(1990) [PubMed: 2198264] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 28-32 AND 146-162. Strain: 86-858 and NY-5.
[2]	"A conserved Streptococcus pyogenes extracellular cysteine protease cleaves human fibronectin and degrades vitronectin." Kapur V., Topouzis S., Majesky M.W., Li L.L., Hamrick M.R., Hamill R.J., Patti J.M., Musser J.M. Microb. Pathog. 15:327-346(1993) [PubMed: 7516997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: 1226 / Serotype M44, 1233 / Serotype M17, 1289 / Serotype M5, 1294 / Serotype M19, 1590, 162 / Serotype M22, 165 / Serotype M, 168 / Serotype M66, 1719 / Serotype T8, 1832 / Serotype M76, 1838 / Serotype M27, 1841 / Serotype M41, 1842 / Serotype M43, 1864 / Serotype M56, 1870, 1871, 1872, 1882 / Serotype M59, 1893, 1896 / Serotype M10, 1898 / Serotype M15, 1901 / Serotype M23, 1911 / Serotype M75, 1914A, 1990 / Serotype M, 1991 / Serotype M, 2017 / Serotype M, 2018 / Serotype M, 262 / Serotype M, 282 / Serotype M12, 289 / Serotype T28, 302 / Serotype M73, 317 / Serotype M, 321 / Serotype M4, 327 / Serotype M2, 366 / Serotype M30, 427 / Serotype M31, 429 / Serotype M8, 650 / Serotype M11, 659 / Serotype M13, 660 / Serotype M14, 684 / Serotype M24, 686 / Serotype M25, 719 / Serotype M49, 758 / Serotype M75, 796 / Serotype M9, 800 / Serotype M9 and 807 / Serotype M33.
[3]	"A novel cloning method used arbitrarily primed PCR." Hong K. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sv / Serotype M23.
[4]	"Primary structure of zymogen of streptococcal proteinase." Yonaha K., Elliott S.D., Liu T.-Y. J. Protein Chem. 1:317-334(1982) Cited for: PRELIMINARY PROTEIN SEQUENCE OF 28-86 AND 121-398.
[5]	"Primary structure of streptococcal proteinase. III. Isolation of cyanogen bromide peptides: complete covalent structure of the polypeptide chain." Tai J.Y., Kortt A.A., Liu T.-Y., Elliott S.D. J. Biol. Chem. 251:1955-1959(1976) [PubMed: 1270417] [Abstract] Cited for: PRELIMINARY PROTEIN SEQUENCE OF 146-398.
[6]	"The mixed disulfide in the zymogen of streptococcal proteinase. Characterization and implication for its biosynthesis." Lo S.S., Fraser B.A., Liu T.-Y. J. Biol. Chem. 259:11041-11045(1984) [PubMed: 6381494] [Abstract] Cited for: CYSTEINE METHYL DISULFIDE.
[7]	"Streptococcal pyrogenic exotoxin B induces apoptosis and reduces phagocytic activity in U937 cells." Kuo C.-F., Wu J.-J., Tsai P.-J., Kao F.-J., Lei H.-Y., Lin M.T., Lin Y.-S. Infect. Immun. 67:126-130(1999) [PubMed: 9864206] [Abstract] Cited for: FUNCTION. Strain: NZ131 / Serotype M49,T14.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M86905 Genomic DNA. Translation: AAA26978.1.
L26126 Genomic DNA. Translation: AAA26992.1.
L26127 Genomic DNA. Translation: AAA26993.1.
L26128 Genomic DNA. Translation: AAA26994.1.
L26129 Genomic DNA. Translation: AAA26995.1.
L26130 Genomic DNA. Translation: AAA26996.1.
L26131 Genomic DNA. Translation: AAA26997.1.
L26132 Genomic DNA. Translation: AAA26998.1.
L26133 Genomic DNA. Translation: AAA26999.1.
L26135 Genomic DNA. Translation: AAA27001.1.
L26136 Genomic DNA. Translation: AAA27002.1.
L26137 Genomic DNA. Translation: AAA27003.1.
L26138 Genomic DNA. Translation: AAA27004.1.
L26139 Genomic DNA. Translation: AAA27005.1.
L26140 Genomic DNA. Translation: AAA27006.1.
L26141 Genomic DNA. Translation: AAA27007.1.
L26142 Genomic DNA. Translation: AAA27008.1.
L26143 Genomic DNA. Translation: AAA27009.1.
L26144 Genomic DNA. Translation: AAA27010.1.
L26145 Genomic DNA. Translation: AAA27011.1.
L26147 Genomic DNA. Translation: AAA27013.1.
L26148 Genomic DNA. Translation: AAA27014.1.
L26149 Genomic DNA. Translation: AAA27015.1.
L26150 Genomic DNA. Translation: AAA27016.1.
L26151 Genomic DNA. Translation: AAA26980.1.
L26152 Genomic DNA. Translation: AAA26981.1.
L26153 Genomic DNA. Translation: AAA26982.1.
L26154 Genomic DNA. Translation: AAA26983.1.
L26155 Genomic DNA. Translation: AAA26984.1.
L26156 Genomic DNA. Translation: AAA26985.1.
L26157 Genomic DNA. Translation: AAA26986.1.
L26159 Genomic DNA. Translation: AAA26988.1.
L26160 Genomic DNA. Translation: AAA26989.1.
L26161 Genomic DNA. Translation: AAA26990.1.
L26162 Genomic DNA. Translation: AAA26991.1.
AB030578 Genomic DNA. Translation: BAB16027.1.

PIR

A37768.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DKI	X-ray	1.60	A/B/C/D	28-398	[»]
1PVJ	X-ray	3.00	A/B/C/D	31-398	[»]

ModBase

Search...

Protein family/group databases

MEROPS

C10.001.

Enzyme and pathway databases

BRENDA

3.4.22.10. 701.

Family and domain databases

InterPro

IPR000200. Peptidase_C10.
[Graphical view]

Pfam

PF01640. Peptidase_C10. 1 hit.
[Graphical view]

PRINTS

PR00797. STREPTOPAIN.

ProDom

PD004169. Peptidase_C10. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Entry information

Entry name

SPEB_STRPY

Accession

Primary (citable) accession number: P0C0J0
Secondary accession number(s): P00788 , P26296, P68883, Q54960, Q54961, Q54962, Q54963, Q54964, Q54965, Q54966, Q54967, Q54968, Q57024, Q57082, Q57202, Q57211, Q57212, Q9S680

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)