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Protein

Streptopain

Gene

speB

Organism
Streptococcus pyogenes
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Important streptococcal virulence factor which cleaves human fibronectin and degrades vitronectin. Also cleaves human IL1B precursor to form biologically active IL1B. Can induce apoptosis in human monocytes and epithelial cells in vitro, and reduces phagocytic activity in monocytic cells. Thus, may play a role in bacterial colonization, invasion, and inhibition of wound healing.1 Publication

Catalytic activityi

Preferential cleavage with hydrophobic residues at P2, P1 and P1'.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei192 – 1921Nucleophile1 Publication
Active sitei340 – 3401Proton acceptor1 Publication

GO - Molecular functioni

GO - Biological processi

  • pathogenesis Source: UniProtKB-KW
  • proteolysis in other organism Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease, Toxin

Keywords - Biological processi

Virulence

Enzyme and pathway databases

BRENDAi3.4.22.10. 5935.
SABIO-RKP0C0J0.

Names & Taxonomyi

Protein namesi
Recommended name:
Streptopain (EC:3.4.22.10)
Alternative name(s):
Exotoxin type B
SPE B
Streptococcal cysteine proteinase
Streptococcus peptidase A
Short name:
SPP
Gene namesi
Name:speB
OrganismiStreptococcus pyogenes
Taxonomic identifieri1314 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2034806.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 27271 PublicationAdd
BLAST
Propeptidei28 – 1451181 PublicationPRO_0000028503Add
BLAST
Chaini146 – 398253StreptopainPRO_0000028504Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei192 – 1921Cysteine methyl disulfide; in zymogen form1 Publication

Keywords - PTMi

Methylation, Zymogen

Proteomic databases

PaxDbiP0C0J0.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi160490.SPy_2039.

Chemistry

BindingDBiP0C0J0.

Structurei

Secondary structure

1
398
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi35 – 384Combined sources
Helixi40 – 478Combined sources
Beta strandi64 – 663Combined sources
Turni71 – 733Combined sources
Beta strandi75 – 828Combined sources
Beta strandi84 – 863Combined sources
Beta strandi88 – 936Combined sources
Beta strandi99 – 1079Combined sources
Helixi115 – 13016Combined sources
Helixi131 – 1333Combined sources
Helixi152 – 1554Combined sources
Turni164 – 1696Combined sources
Helixi192 – 20413Combined sources
Beta strandi214 – 2174Combined sources
Beta strandi230 – 2334Combined sources
Helixi235 – 2373Combined sources
Turni242 – 2443Combined sources
Helixi255 – 27117Combined sources
Beta strandi277 – 2793Combined sources
Helixi285 – 29410Combined sources
Beta strandi303 – 3064Combined sources
Helixi307 – 3093Combined sources
Helixi312 – 32413Combined sources
Beta strandi329 – 3357Combined sources
Beta strandi338 – 35114Combined sources
Beta strandi353 – 3564Combined sources
Turni360 – 3634Combined sources
Beta strandi365 – 3684Combined sources
Helixi375 – 3773Combined sources
Beta strandi388 – 3947Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DKIX-ray1.60A/B/C/D28-398[»]
1PVJX-ray3.00A/B/C/D31-398[»]
2UZJX-ray1.55A/B146-398[»]
ProteinModelPortaliP0C0J0.
SMRiP0C0J0. Positions 31-398.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0C0J0.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C10 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105JBQ. Bacteria.
ENOG4111S8V. LUCA.

Family and domain databases

InterProiIPR000200. Peptidase_C10.
IPR025896. Spi_Prtas-inh.
[Graphical view]
PfamiPF13734. Inhibitor_I69. 1 hit.
PF01640. Peptidase_C10. 1 hit.
[Graphical view]
PRINTSiPR00797. STREPTOPAIN.
ProDomiPD004169. Peptidase_C10. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C0J0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKKKLGIRL LSLLALGGFV LANPVFADQN FARNEKEAKD SAITFIQKSA
60 70 80 90 100
AIKAGARSAE DIKLDKVNLG GELSGSNMYV YNISTGGFVI VSGDKRSPEI
110 120 130 140 150
LGYSTSGSFD ANGKENIASF MESYVEQIKE NKKLDTTYAG TAEIKQPVVK
160 170 180 190 200
SLLDSKGIHY NQGNPYNLLT PVIEKVKPGE QSFVGQHAAT GCVATATAQI
210 220 230 240 250
MKYHNYPNKG LKDYTYTLSS NNPYFNHPKN LFAAISTRQY NWNNILPTYS
260 270 280 290 300
GRESNVQKMA ISELMADVGI SVDMDYGPSS GSAGSSRVQR ALKENFGYNQ
310 320 330 340 350
SVHQINRSDF SKQDWEAQID KELSQNQPVY YQGVGKVGGH AFVIDGADGR
360 370 380 390
NFYHVNWGWG GVSDGFFRLD ALNPSALGTG GGAGGFNGYQ SAVVGIKP
Length:398
Mass (Da):43,174
Last modified:September 13, 2005 - v1
Checksum:i16FF180D720AEE0F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti84 – 852ST → AS AA sequence (Ref. 4) Curated
Sequence conflicti169 – 1691L → I AA sequence (Ref. 4) Curated
Sequence conflicti187 – 1915HAATG → AATGH AA sequence (Ref. 4) Curated
Sequence conflicti187 – 1915HAATG → AATGH AA sequence (PubMed:1270417).Curated
Sequence conflicti208 – 2081N → D AA sequence (Ref. 4) Curated
Sequence conflicti208 – 2081N → D AA sequence (PubMed:1270417).Curated
Sequence conflicti213 – 2131D → N AA sequence (Ref. 4) Curated
Sequence conflicti213 – 2131D → N AA sequence (PubMed:1270417).Curated
Sequence conflicti222 – 2232NP → PD AA sequence (Ref. 4) Curated
Sequence conflicti222 – 2232NP → PD AA sequence (PubMed:1270417).Curated
Sequence conflicti226 – 2261N → D AA sequence (Ref. 4) Curated
Sequence conflicti226 – 2261N → D AA sequence (PubMed:1270417).Curated
Sequence conflicti241 – 2411N → D AA sequence (Ref. 4) Curated
Sequence conflicti241 – 2411N → D AA sequence (PubMed:1270417).Curated
Sequence conflicti253 – 2575ESNVQ → QSQNV AA sequence (Ref. 4) Curated
Sequence conflicti253 – 2575ESNVQ → QSQNV AA sequence (PubMed:1270417).Curated
Sequence conflicti306 – 3061N → D AA sequence (Ref. 4) Curated
Sequence conflicti332 – 3321Q → E AA sequence (Ref. 4) Curated
Sequence conflicti332 – 3321Q → E AA sequence (PubMed:1270417).Curated
Sequence conflicti346 – 3483GAD → DGA AA sequence (Ref. 4) Curated
Sequence conflicti346 – 3483GAD → DGA AA sequence (PubMed:1270417).Curated
Sequence conflicti356 – 3561N → D AA sequence (Ref. 4) Curated
Sequence conflicti356 – 3561N → D AA sequence (PubMed:1270417).Curated
Sequence conflicti390 – 3901Q → E AA sequence (Ref. 4) Curated
Sequence conflicti390 – 3901Q → E AA sequence (PubMed:1270417).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti17 – 171G → S in strain: MGAS 1896.
Natural varianti80 – 801V → I in strain: MGAS 168.
Natural varianti111 – 1111A → V in strain: MGAS 165, 168, 429, 659, 660, 796, 800, 1719, 1838, 1882, 2017 and 2018.
Natural varianti137 – 1371T → I in strain: MGAS 650.
Natural varianti154 – 1541D → N in strain: MGAS 684.
Natural varianti211 – 2111L → V in strain: MGAS 366, 427, 758, 1294, 1911, 1914A and 1991.
Natural varianti305 – 3051I → V in strain: MGAS 1901 and Sv.
Natural varianti308 – 3081S → G in strain: MGAS 429, 659, 807, 1226, 1719, 1832, 1842, 1871, 1872, 2017 and 2018.
Natural varianti317 – 3171A → S in strain: MGAS 165, 168, 289, 302, 1233 and 1898.
Natural varianti384 – 3841G → D in strain: MGAS 1871.
Natural varianti394 – 3941V → I in strain: MGAS 366 and 1294.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86905 Genomic DNA. Translation: AAA26978.1.
L26126 Genomic DNA. Translation: AAA26992.1.
L26127 Genomic DNA. Translation: AAA26993.1.
L26128 Genomic DNA. Translation: AAA26994.1.
L26129 Genomic DNA. Translation: AAA26995.1.
L26130 Genomic DNA. Translation: AAA26996.1.
L26131 Genomic DNA. Translation: AAA26997.1.
L26132 Genomic DNA. Translation: AAA26998.1.
L26133 Genomic DNA. Translation: AAA26999.1.
L26135 Genomic DNA. Translation: AAA27001.1.
L26136 Genomic DNA. Translation: AAA27002.1.
L26137 Genomic DNA. Translation: AAA27003.1.
L26138 Genomic DNA. Translation: AAA27004.1.
L26139 Genomic DNA. Translation: AAA27005.1.
L26140 Genomic DNA. Translation: AAA27006.1.
L26141 Genomic DNA. Translation: AAA27007.1.
L26142 Genomic DNA. Translation: AAA27008.1.
L26143 Genomic DNA. Translation: AAA27009.1.
L26144 Genomic DNA. Translation: AAA27010.1.
L26145 Genomic DNA. Translation: AAA27011.1.
L26147 Genomic DNA. Translation: AAA27013.1.
L26148 Genomic DNA. Translation: AAA27014.1.
L26149 Genomic DNA. Translation: AAA27015.1.
L26150 Genomic DNA. Translation: AAA27016.1.
L26151 Genomic DNA. Translation: AAA26980.1.
L26152 Genomic DNA. Translation: AAA26981.1.
L26153 Genomic DNA. Translation: AAA26982.1.
L26154 Genomic DNA. Translation: AAA26983.1.
L26155 Genomic DNA. Translation: AAA26984.1.
L26156 Genomic DNA. Translation: AAA26985.1.
L26157 Genomic DNA. Translation: AAA26986.1.
L26159 Genomic DNA. Translation: AAA26988.1.
L26160 Genomic DNA. Translation: AAA26989.1.
L26161 Genomic DNA. Translation: AAA26990.1.
L26162 Genomic DNA. Translation: AAA26991.1.
AB030578 Genomic DNA. Translation: BAB16027.1.
PIRiA37768.
RefSeqiWP_002991253.1. NZ_JHTN01000084.1.
WP_009881074.1. NZ_CP010449.1.
WP_011285235.1. NZ_CP014139.1.
WP_014407896.1. NZ_CP007562.1.
WP_031488619.1. NZ_HG316453.1.
WP_038433740.1. NZ_CP008695.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86905 Genomic DNA. Translation: AAA26978.1.
L26126 Genomic DNA. Translation: AAA26992.1.
L26127 Genomic DNA. Translation: AAA26993.1.
L26128 Genomic DNA. Translation: AAA26994.1.
L26129 Genomic DNA. Translation: AAA26995.1.
L26130 Genomic DNA. Translation: AAA26996.1.
L26131 Genomic DNA. Translation: AAA26997.1.
L26132 Genomic DNA. Translation: AAA26998.1.
L26133 Genomic DNA. Translation: AAA26999.1.
L26135 Genomic DNA. Translation: AAA27001.1.
L26136 Genomic DNA. Translation: AAA27002.1.
L26137 Genomic DNA. Translation: AAA27003.1.
L26138 Genomic DNA. Translation: AAA27004.1.
L26139 Genomic DNA. Translation: AAA27005.1.
L26140 Genomic DNA. Translation: AAA27006.1.
L26141 Genomic DNA. Translation: AAA27007.1.
L26142 Genomic DNA. Translation: AAA27008.1.
L26143 Genomic DNA. Translation: AAA27009.1.
L26144 Genomic DNA. Translation: AAA27010.1.
L26145 Genomic DNA. Translation: AAA27011.1.
L26147 Genomic DNA. Translation: AAA27013.1.
L26148 Genomic DNA. Translation: AAA27014.1.
L26149 Genomic DNA. Translation: AAA27015.1.
L26150 Genomic DNA. Translation: AAA27016.1.
L26151 Genomic DNA. Translation: AAA26980.1.
L26152 Genomic DNA. Translation: AAA26981.1.
L26153 Genomic DNA. Translation: AAA26982.1.
L26154 Genomic DNA. Translation: AAA26983.1.
L26155 Genomic DNA. Translation: AAA26984.1.
L26156 Genomic DNA. Translation: AAA26985.1.
L26157 Genomic DNA. Translation: AAA26986.1.
L26159 Genomic DNA. Translation: AAA26988.1.
L26160 Genomic DNA. Translation: AAA26989.1.
L26161 Genomic DNA. Translation: AAA26990.1.
L26162 Genomic DNA. Translation: AAA26991.1.
AB030578 Genomic DNA. Translation: BAB16027.1.
PIRiA37768.
RefSeqiWP_002991253.1. NZ_JHTN01000084.1.
WP_009881074.1. NZ_CP010449.1.
WP_011285235.1. NZ_CP014139.1.
WP_014407896.1. NZ_CP007562.1.
WP_031488619.1. NZ_HG316453.1.
WP_038433740.1. NZ_CP008695.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DKIX-ray1.60A/B/C/D28-398[»]
1PVJX-ray3.00A/B/C/D31-398[»]
2UZJX-ray1.55A/B146-398[»]
ProteinModelPortaliP0C0J0.
SMRiP0C0J0. Positions 31-398.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi160490.SPy_2039.

Chemistry

BindingDBiP0C0J0.
ChEMBLiCHEMBL2034806.

Proteomic databases

PaxDbiP0C0J0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105JBQ. Bacteria.
ENOG4111S8V. LUCA.

Enzyme and pathway databases

BRENDAi3.4.22.10. 5935.
SABIO-RKP0C0J0.

Miscellaneous databases

EvolutionaryTraceiP0C0J0.

Family and domain databases

InterProiIPR000200. Peptidase_C10.
IPR025896. Spi_Prtas-inh.
[Graphical view]
PfamiPF13734. Inhibitor_I69. 1 hit.
PF01640. Peptidase_C10. 1 hit.
[Graphical view]
PRINTSiPR00797. STREPTOPAIN.
ProDomiPD004169. Peptidase_C10. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Entry informationi

Entry nameiSPEB_STRPY
AccessioniPrimary (citable) accession number: P0C0J0
Secondary accession number(s): P00788
, P26296, P68883, Q54960, Q54961, Q54962, Q54963, Q54964, Q54965, Q54966, Q54967, Q54968, Q57024, Q57082, Q57202, Q57211, Q57212, Q9S680
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: September 13, 2005
Last modified: June 8, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.