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P0C0H1 (HASA_STRP1) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Hyaluronan synthase
EC=2.4.1.212
Alternative name(s):
Hyaluronate synthase
Hyaluronic acid synthase
Short name=HA synthase
Gene names
Name:hasA
Ordered Locus Names:SPy_2200, M5005_Spy1851
OrganismStreptococcus pyogenes serotype M1 [Complete proteome] [HAMAP]
Taxonomic identifier301447 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length419 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Glycosaminoglycan synthesis. The hyaluronic acid capsule is involved in the pathogenicity of group A Streptococci; it may be the major virulence determinant By similarity.

Catalytic activity

UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan).

UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan).

Cofactor

Magnesium By similarity.

Pathway

Glycan biosynthesis; hyaluronan biosynthesis.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the nodC/HAS family.

Caution

It is uncertain whether Met-1 or Met-25 is the initiator.

Ontologies

Keywords
   Biological processCapsule biogenesis/degradation
Virulence
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processpathogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionhyaluronan synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 419419Hyaluronan synthase
PRO_0000197165

Regions

Transmembrane8 – 2821Helical; Potential
Transmembrane33 – 5321Helical; Potential
Transmembrane318 – 33821Helical; Potential
Transmembrane345 – 36521Helical; Potential
Transmembrane376 – 39621Helical; Potential

Experimental info

Sequence conflict101V → I in AAZ52469. Ref.2
Sequence conflict3041K → R in AAZ52469. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P0C0H1 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: A6AFE04B54B8D385

FASTA41947,886
        10         20         30         40         50         60 
MPIFKKTLIV LSFIFLISIL IYLNMYLFGT STVGIYGVIL ITYLVIKLGL SFLYEPFKGK 

        70         80         90        100        110        120 
PHDYKVAAVI PSYNEDAESL LETLKSVLAQ TYPLSEIYIV DDGSSNTDAI QLIEEYVNRE 

       130        140        150        160        170        180 
VDICRNVIVH RSLVNKGKRH AQAWAFERSD ADVFLTVDSD TYIYPNALEE LLKSFNDETV 

       190        200        210        220        230        240 
YAATGHLNAR NRQTNLLTRL TDIRYDNAFG VERAAQSLTG NILVCSGPLS IYRREVIIPN 

       250        260        270        280        290        300 
LERYKNQTFL GLPVSIGDDR CLTNYAIDLG RTVYQSTARC DTDVPFQLKS YLKQQNRWNK 

       310        320        330        340        350        360 
SFFKESIISV KKILSNPIVA LWTIFEVVMF MMLIVAIGNL LFNQAIQLDL IKLFAFLSII 

       370        380        390        400        410 
FIVALCRNVH YMIKHPASFL LSPLYGILHL FVLQPLKLYS LCTIKNTEWG TRKKVTIFK

« Hide

References

[1]"Complete genome sequence of an M1 strain of Streptococcus pyogenes."
Ferretti J.J., McShan W.M., Ajdic D.J., Savic D.J., Savic G., Lyon K., Primeaux C., Sezate S., Suvorov A.N., Kenton S., Lai H.S., Lin S.P., Qian Y., Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L. expand/collapse author list , White J., Yuan X., Clifton S.W., Roe B.A., McLaughlin R.E.
Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001) [PubMed: 11296296] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700294 / SF370 / Serotype M1.
[2]"Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events."
Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M.
J. Infect. Dis. 192:771-782(2005) [PubMed: 16088826] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE004092 Genomic DNA. Translation: AAK34828.1.
CP000017 Genomic DNA. Translation: AAZ52469.1.
RefSeqNP_270107.1. NC_002737.1.
YP_283214.1. NC_007297.1.

3D structure databases

ProteinModelPortalP0C0H1.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBSTRT00000000608; EBSTRP00000000608; EBSTRG00000000608.
EBSTRT00000027847; EBSTRP00000026865; EBSTRG00000027847.
GeneID3571023.
901875.
GenomeReviewsGene locus SPy_2200 in contig AE004092_GR.
Gene locus M5005_Spy1851 in contig CP000017_GR.
KEGGspy:SPy_2200.
spz:M5005_Spy_1851.
PATRIC19717723. VBIStrPyo79812_1905.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000026831.
HOGENOMHBG339192.
OMAWYHQTFL.
ProtClustDBCLSK687618.

Enzyme and pathway databases

BioCycSPYO160490:SPY2200-MONOMER.
SPYO293653:M5005_SPY1851-MONOMER.

Family and domain databases

InterProIPR001173. Glyco_trans_2.
[Graphical view]
KOK00752.
PfamPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHASA_STRP1
AccessionPrimary (citable) accession number: P0C0H1
Secondary accession number(s): Q08494 expand/collapse secondary AC list , Q48W06, Q54865, Q54868
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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