P0C0H0 (HASA_STRPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hyaluronan synthase
EC=2.4.1.212

Alternative name(s):
Hyaluronate synthase
Hyaluronic acid synthase
Short name=HA synthase

Gene names

Name:

hasA

Organism

Streptococcus pyogenes

Taxonomic identifier

1314 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Glycosaminoglycan synthesis. The hyaluronic acid capsule is involved in the pathogenicity of group A Streptococci; it may be the major virulence determinant.
Catalytic activity	UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan). UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan).
Cofactor	Magnesium.
Pathway	Glycan biosynthesis; hyaluronan biosynthesis.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the nodC/HAS family.
Caution	It is uncertain whether Met-1 or Met-25 is the initiator.

Ontologies

Keywords
Biological process	Capsule biogenesis/degradation Virulence
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hyaluronan synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	Hyaluronan synthase		PRO_0000197164
Regions
	Transmembrane	8 – 28	21	Helical; Potential
	Transmembrane	33 – 53	21	Helical; Potential
	Transmembrane	318 – 338	21	Helical; Potential
	Transmembrane	345 – 365	21	Helical; Potential
	Transmembrane	376 – 396	21	Helical; Potential
Experimental info
	Sequence conflict	60	1	K → N Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0C0H0 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: EB173041DA93A71B	FASTA	419	47,900
	10 20 30 40 50 60 MPIFKKTLIV LSFIFLISIL IYLNMYLFGT STVGIYGVIL ITYLVIKLGL SFLYEPFKGK 70 80 90 100 110 120 PHDYKVAAVI PSYNEDAESL LETLKSVLAQ TYPLSEIYIV DDGSSNTDAI QLIEEYVNRE 130 140 150 160 170 180 VDICRNVIVH RSLVNKGKRH AQAWAFERSD ADVFLTVDSD TYIYPNALEE LLKSFNDETV 190 200 210 220 230 240 YAATGHLNAR NRQTNLLTRL TDIRYDNAFG VERAAQSLTG NILVCSGPLS IYRREVIIPN 250 260 270 280 290 300 LERYKNQTFL GLPVSIGDDR CLTNYAIDLG RTVYQSTARC DTDVPFQLKS YLKQQNRWNK 310 320 330 340 350 360 SFFRESIISV KKILSNPIVA LWTIFEVVMF MMLIVAIGNL LFNQAIQLDL IKLFAFLSII 370 380 390 400 410 FIVALCRNVH YMVKHPASFL LSPLYGILHL FVLQPLKLYS LCTIKNTEWG TRKKVTIFK

References

[1]	"Molecular characterization of hasA from an operon required for hyaluronic acid synthesis in group A streptococci." Dougherty B.A., van de Rijn I. J. Biol. Chem. 269:169-175(1994) [PubMed: 8276791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: WF50.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L21187 Unassigned DNA. Translation: AAA17725.1.
3D structure databases
ProteinModelPortal	P0C0H0.
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
TCDB	9.B.32.1.4. putative vectorial glycosyl polymerization (VGP) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001173. Glyco_trans_2. [Graphical view]
Pfam	PF00535. Glycos_transf_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HASA_STRPY

Accession

Primary (citable) accession number: P0C0H0
Secondary accession number(s): Q08494, Q54865, Q54868

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	September 13, 2005
Last modified:	June 28, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program