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P0C0F4

- UDG_STRPY

UniProt

P0C0F4 - UDG_STRPY

Protein

UDP-glucose 6-dehydrogenase

Gene

hasB

Organism
Streptococcus pyogenes
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of UDP-glucuronic acid which is required for capsular hyaluronic acid synthesis.

    Catalytic activityi

    UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH.

    Kineticsi

    1. KM=20 µM for UDP-glucose
    2. KM=65 µM for NAD

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei29 – 291NAD1 Publication
    Binding sitei34 – 341NAD1 Publication
    Binding sitei83 – 831NAD1 Publication
    Binding sitei118 – 1181NAD; via amide nitrogen1 Publication
    Binding sitei145 – 1451NAD1 Publication
    Binding sitei204 – 2041Substrate1 Publication
    Binding sitei257 – 2571Substrate; via amide nitrogen1 Publication
    Active sitei260 – 2601Nucleophile
    Binding sitei263 – 2631NAD1 Publication
    Binding sitei319 – 3191Substrate; via carbonyl oxygen1 Publication
    Binding sitei320 – 3201Substrate1 Publication
    Binding sitei327 – 3271NAD1 Publication
    Binding sitei402 – 4021Substrate1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi2 – 1918NAD1 PublicationAdd
    BLAST

    GO - Molecular functioni

    1. NAD binding Source: InterPro
    2. UDP-glucose 6-dehydrogenase activity Source: CACAO

    GO - Biological processi

    1. polysaccharide biosynthetic process Source: InterPro
    2. UDP-glucuronate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Capsule biogenesis/degradation

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    SABIO-RKP0C0F4.
    UniPathwayiUPA00038; UER00491.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-glucose 6-dehydrogenase (EC:1.1.1.22)
    Short name:
    UDP-Glc dehydrogenase
    Short name:
    UDP-GlcDH
    Short name:
    UDPGDH
    Gene namesi
    Name:hasB
    OrganismiStreptococcus pyogenes
    Taxonomic identifieri1314 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi118 – 1181T → A: Increases Km for substrate 3-fold. Increases Km for NAD 6-fold. Reduces catalytic activity 160-fold. 1 Publication
    Mutagenesisi141 – 1411E → Q: Increases Km for substrate 3-fold. Increases Km for NAD 2-fold. Reduces catalytic activity about 10-fold. 1 Publication
    Mutagenesisi145 – 1451E → Q: Increases Km for substrate 6-fold. Increases Km for NAD 3-fold. Reduces catalytic activity about 10-fold. 1 Publication
    Mutagenesisi260 – 2601C → A or S: Loss of activity. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 402402UDP-glucose 6-dehydrogenasePRO_0000074054Add
    BLAST

    Structurei

    Secondary structure

    1
    402
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 65
    Helixi10 – 1910
    Turni20 – 223
    Beta strandi23 – 286
    Helixi32 – 398
    Helixi48 – 569
    Beta strandi61 – 655
    Helixi67 – 737
    Beta strandi75 – 795
    Turni87 – 904
    Helixi95 – 10713
    Beta strandi112 – 1154
    Helixi123 – 1308
    Beta strandi136 – 1383
    Helixi150 – 1534
    Beta strandi158 – 1614
    Helixi168 – 18417
    Beta strandi186 – 1883
    Beta strandi192 – 1954
    Helixi197 – 22731
    Helixi232 – 2409
    Turni243 – 2453
    Beta strandi247 – 2493
    Helixi260 – 27213
    Helixi279 – 30224
    Beta strandi311 – 3155
    Helixi330 – 33910
    Beta strandi344 – 3485
    Beta strandi361 – 3633
    Helixi367 – 3737
    Beta strandi375 – 3784
    Helixi384 – 3929

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1DLIX-ray2.31A1-402[»]
    1DLJX-ray1.80A1-402[»]
    1LTYmodel-A1-402[»]
    ProteinModelPortaliP0C0F4.
    SMRiP0C0F4. Positions 1-402.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0C0F4.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni142 – 1454Substrate binding
    Regioni249 – 2535Substrate binding

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1004.

    Family and domain databases

    Gene3Di1.10.1040.10. 1 hit.
    3.40.50.720. 2 hits.
    InterProiIPR008927. 6-PGluconate_DH_C-like.
    IPR013328. DH_multihelical.
    IPR016040. NAD(P)-bd_dom.
    IPR017476. UDP-Glc/GDP-Man.
    IPR014027. UDP-Glc/GDP-Man_DH_C.
    IPR014026. UDP-Glc/GDP-Man_DH_dimer.
    IPR001732. UDP-Glc/GDP-Man_DH_N.
    IPR028357. UDPglc_DH_bac.
    [Graphical view]
    PANTHERiPTHR11374. PTHR11374. 1 hit.
    PfamiPF00984. UDPG_MGDP_dh. 1 hit.
    PF03720. UDPG_MGDP_dh_C. 1 hit.
    PF03721. UDPG_MGDP_dh_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF500134. UDPglc_DH_bac. 1 hit.
    PIRSF000124. UDPglc_GDPman_dh. 1 hit.
    SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF48179. SSF48179. 1 hit.
    SSF52413. SSF52413. 1 hit.
    TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0C0F4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKIAVAGSGY VGLSLGVLLS LQNEVTIVDI LPSKVDKINN GLSPIQDEYI    50
    EYYLKSKQLS IKATLDSKAA YKEAELVIIA TPTNYNSRIN YFDTQHVETV 100
    IKEVLSVNSH ATLIIKSTIP IGFITEMRQK FQTDRIIFSP EFLRESKALY 150
    DNLYPSRIIV SCEENDSPKV KADAEKFALL LKSAAKKNNV PVLIMGASEA 200
    EAVKLFANTY LALRVAYFNE LDTYAESRKL NSHMIIQGIS YDDRIGMHYN 250
    NPSFGYGGYC LPKDTKQLLA NYNNIPQTLI EAIVSSNNVR KSYIAKQIIN 300
    VLKEQESPVK VVGVYRLIMK SNSDNFRESA IKDVIDILKS KDIKIIIYEP 350
    MLNKLESEDQ SVLVNDLENF KKQANIIVTN RYDNELQDVK NKVYSRDIFG 400
    RD 402
    Length:402
    Mass (Da):45,483
    Last modified:September 13, 2005 - v1
    Checksum:iDD1869D659F4CA58
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L08444 Genomic DNA. Translation: AAA26899.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L08444 Genomic DNA. Translation: AAA26899.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1DLI X-ray 2.31 A 1-402 [» ]
    1DLJ X-ray 1.80 A 1-402 [» ]
    1LTY model - A 1-402 [» ]
    ProteinModelPortali P0C0F4.
    SMRi P0C0F4. Positions 1-402.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG1004.

    Enzyme and pathway databases

    UniPathwayi UPA00038 ; UER00491 .
    SABIO-RK P0C0F4.

    Miscellaneous databases

    EvolutionaryTracei P0C0F4.

    Family and domain databases

    Gene3Di 1.10.1040.10. 1 hit.
    3.40.50.720. 2 hits.
    InterProi IPR008927. 6-PGluconate_DH_C-like.
    IPR013328. DH_multihelical.
    IPR016040. NAD(P)-bd_dom.
    IPR017476. UDP-Glc/GDP-Man.
    IPR014027. UDP-Glc/GDP-Man_DH_C.
    IPR014026. UDP-Glc/GDP-Man_DH_dimer.
    IPR001732. UDP-Glc/GDP-Man_DH_N.
    IPR028357. UDPglc_DH_bac.
    [Graphical view ]
    PANTHERi PTHR11374. PTHR11374. 1 hit.
    Pfami PF00984. UDPG_MGDP_dh. 1 hit.
    PF03720. UDPG_MGDP_dh_C. 1 hit.
    PF03721. UDPG_MGDP_dh_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF500134. UDPglc_DH_bac. 1 hit.
    PIRSF000124. UDPglc_GDPman_dh. 1 hit.
    SMARTi SM00984. UDPG_MGDP_dh_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48179. SSF48179. 1 hit.
    SSF52413. SSF52413. 1 hit.
    TIGRFAMsi TIGR03026. NDP-sugDHase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of hasB from an operon required for hyaluronic acid synthesis in group A streptococci. Demonstration of UDP-glucose dehydrogenase activity."
      Dougherty B.A., van de Rijn I.
      J. Biol. Chem. 268:7118-7124(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: WF50.
    2. "Active site residues and mechanism of UDP-glucose dehydrogenase."
      Ge X., Penney L.C., van de Rijn I., Tanner M.E.
      Eur. J. Biochem. 271:14-22(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF THR-118; GLU-141; GLU-145 AND CYS-260.
    3. "The first structure of UDP-glucose dehydrogenase reveals the catalytic residues necessary for the two-fold oxidation."
      Campbell R.E., Mosimann S.C., van De Rijn I., Tanner M.E., Strynadka N.C.
      Biochemistry 39:7012-7023(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.31 ANGSTROMS) IN COMPLEX WITH SUBSTRATE AND NAD, MUTAGENESIS OF CYS-260.

    Entry informationi

    Entry nameiUDG_STRPY
    AccessioniPrimary (citable) accession number: P0C0F4
    Secondary accession number(s): Q07172
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2005
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3