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Protein

UDP-glucose 6-dehydrogenase

Gene

hasB

Organism
Streptococcus pyogenes
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of UDP-glucuronic acid which is required for capsular hyaluronic acid synthesis.1 Publication

Catalytic activityi

UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH.1 Publication

Kineticsi

  1. KM=20 µM for UDP-glucose1 Publication
  2. KM=65 µM for NAD1 Publication

    Pathwayi: UDP-alpha-D-glucuronate biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes UDP-alpha-D-glucuronate from UDP-alpha-D-glucose.
    Proteins known to be involved in this subpathway in this organism are:
    1. UDP-glucose 6-dehydrogenase (hasB)
    This subpathway is part of the pathway UDP-alpha-D-glucuronate biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP-alpha-D-glucuronate from UDP-alpha-D-glucose, the pathway UDP-alpha-D-glucuronate biosynthesis and in Nucleotide-sugar biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei29NAD1 Publication1
    Binding sitei34NAD1 Publication1
    Binding sitei83NAD1 Publication1
    Binding sitei118NAD; via amide nitrogen1 Publication1
    Binding sitei145NAD1 Publication1
    Binding sitei204Substrate1 Publication1
    Binding sitei257Substrate; via amide nitrogen1 Publication1
    Active sitei260Nucleophile1 Publication1
    Binding sitei263NAD1 Publication1
    Binding sitei319Substrate; via carbonyl oxygen1 Publication1
    Binding sitei320Substrate1 Publication1
    Binding sitei327NAD1 Publication1
    Binding sitei402Substrate1 Publication1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi2 – 19NAD1 PublicationAdd BLAST18

    GO - Molecular functioni

    • NAD binding Source: InterPro
    • UDP-glucose 6-dehydrogenase activity Source: CACAO

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Capsule biogenesis/degradation

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    SABIO-RKP0C0F4.
    UniPathwayiUPA00038; UER00491.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-glucose 6-dehydrogenase (EC:1.1.1.221 Publication)
    Short name:
    UDP-Glc dehydrogenase
    Short name:
    UDP-GlcDH
    Short name:
    UDPGDH
    Gene namesi
    Name:hasB
    OrganismiStreptococcus pyogenes
    Taxonomic identifieri1314 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi118T → A: Increases Km for substrate 3-fold. Increases Km for NAD 6-fold. Reduces catalytic activity 160-fold. 1 Publication1
    Mutagenesisi141E → Q: Increases Km for substrate 3-fold. Increases Km for NAD 2-fold. Reduces catalytic activity about 10-fold. 1 Publication1
    Mutagenesisi145E → Q: Increases Km for substrate 6-fold. Increases Km for NAD 3-fold. Reduces catalytic activity about 10-fold. 1 Publication1
    Mutagenesisi260C → A or S: Loss of activity. 2 Publications1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000740541 – 402UDP-glucose 6-dehydrogenaseAdd BLAST402

    Proteomic databases

    PaxDbiP0C0F4.

    Interactioni

    Protein-protein interaction databases

    STRINGi160490.SPy_2201.

    Structurei

    Secondary structure

    1402
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi2 – 6Combined sources5
    Helixi10 – 19Combined sources10
    Turni20 – 22Combined sources3
    Beta strandi23 – 28Combined sources6
    Helixi32 – 39Combined sources8
    Helixi48 – 56Combined sources9
    Beta strandi61 – 65Combined sources5
    Helixi67 – 73Combined sources7
    Beta strandi75 – 79Combined sources5
    Turni87 – 90Combined sources4
    Helixi95 – 107Combined sources13
    Beta strandi112 – 115Combined sources4
    Helixi123 – 130Combined sources8
    Beta strandi136 – 138Combined sources3
    Helixi150 – 153Combined sources4
    Beta strandi158 – 161Combined sources4
    Helixi168 – 184Combined sources17
    Beta strandi186 – 188Combined sources3
    Beta strandi192 – 195Combined sources4
    Helixi197 – 227Combined sources31
    Helixi232 – 240Combined sources9
    Turni243 – 245Combined sources3
    Beta strandi247 – 249Combined sources3
    Helixi260 – 272Combined sources13
    Helixi279 – 302Combined sources24
    Beta strandi311 – 315Combined sources5
    Helixi330 – 339Combined sources10
    Beta strandi344 – 348Combined sources5
    Beta strandi361 – 363Combined sources3
    Helixi367 – 373Combined sources7
    Beta strandi375 – 378Combined sources4
    Helixi384 – 392Combined sources9

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1DLIX-ray2.31A1-402[»]
    1DLJX-ray1.80A1-402[»]
    1LTYmodel-A1-402[»]
    ProteinModelPortaliP0C0F4.
    SMRiP0C0F4.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0C0F4.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni142 – 145Substrate bindingCombined sources1 Publication4
    Regioni249 – 253Substrate bindingCombined sources1 Publication5

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1004. LUCA.

    Family and domain databases

    Gene3Di1.10.1040.10. 1 hit.
    3.40.50.720. 2 hits.
    InterProiIPR008927. 6-PGluconate_DH_C-like.
    IPR013328. 6PGD_dom_2.
    IPR016040. NAD(P)-bd_dom.
    IPR017476. UDP-Glc/GDP-Man.
    IPR014027. UDP-Glc/GDP-Man_DH_C.
    IPR014026. UDP-Glc/GDP-Man_DH_dimer.
    IPR001732. UDP-Glc/GDP-Man_DH_N.
    IPR028357. UDPglc_DH_bac.
    [Graphical view]
    PANTHERiPTHR11374. PTHR11374. 1 hit.
    PfamiPF00984. UDPG_MGDP_dh. 1 hit.
    PF03720. UDPG_MGDP_dh_C. 1 hit.
    PF03721. UDPG_MGDP_dh_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF500134. UDPglc_DH_bac. 1 hit.
    PIRSF000124. UDPglc_GDPman_dh. 1 hit.
    SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF48179. SSF48179. 1 hit.
    SSF51735. SSF51735. 1 hit.
    SSF52413. SSF52413. 1 hit.
    TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0C0F4-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKIAVAGSGY VGLSLGVLLS LQNEVTIVDI LPSKVDKINN GLSPIQDEYI
    60 70 80 90 100
    EYYLKSKQLS IKATLDSKAA YKEAELVIIA TPTNYNSRIN YFDTQHVETV
    110 120 130 140 150
    IKEVLSVNSH ATLIIKSTIP IGFITEMRQK FQTDRIIFSP EFLRESKALY
    160 170 180 190 200
    DNLYPSRIIV SCEENDSPKV KADAEKFALL LKSAAKKNNV PVLIMGASEA
    210 220 230 240 250
    EAVKLFANTY LALRVAYFNE LDTYAESRKL NSHMIIQGIS YDDRIGMHYN
    260 270 280 290 300
    NPSFGYGGYC LPKDTKQLLA NYNNIPQTLI EAIVSSNNVR KSYIAKQIIN
    310 320 330 340 350
    VLKEQESPVK VVGVYRLIMK SNSDNFRESA IKDVIDILKS KDIKIIIYEP
    360 370 380 390 400
    MLNKLESEDQ SVLVNDLENF KKQANIIVTN RYDNELQDVK NKVYSRDIFG

    RD
    Length:402
    Mass (Da):45,483
    Last modified:September 13, 2005 - v1
    Checksum:iDD1869D659F4CA58
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L08444 Genomic DNA. Translation: AAA26899.1.
    RefSeqiWP_011018341.1. NZ_JHTN01000039.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L08444 Genomic DNA. Translation: AAA26899.1.
    RefSeqiWP_011018341.1. NZ_JHTN01000039.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1DLIX-ray2.31A1-402[»]
    1DLJX-ray1.80A1-402[»]
    1LTYmodel-A1-402[»]
    ProteinModelPortaliP0C0F4.
    SMRiP0C0F4.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi160490.SPy_2201.

    Proteomic databases

    PaxDbiP0C0F4.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Phylogenomic databases

    eggNOGiCOG1004. LUCA.

    Enzyme and pathway databases

    UniPathwayiUPA00038; UER00491.
    SABIO-RKP0C0F4.

    Miscellaneous databases

    EvolutionaryTraceiP0C0F4.

    Family and domain databases

    Gene3Di1.10.1040.10. 1 hit.
    3.40.50.720. 2 hits.
    InterProiIPR008927. 6-PGluconate_DH_C-like.
    IPR013328. 6PGD_dom_2.
    IPR016040. NAD(P)-bd_dom.
    IPR017476. UDP-Glc/GDP-Man.
    IPR014027. UDP-Glc/GDP-Man_DH_C.
    IPR014026. UDP-Glc/GDP-Man_DH_dimer.
    IPR001732. UDP-Glc/GDP-Man_DH_N.
    IPR028357. UDPglc_DH_bac.
    [Graphical view]
    PANTHERiPTHR11374. PTHR11374. 1 hit.
    PfamiPF00984. UDPG_MGDP_dh. 1 hit.
    PF03720. UDPG_MGDP_dh_C. 1 hit.
    PF03721. UDPG_MGDP_dh_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF500134. UDPglc_DH_bac. 1 hit.
    PIRSF000124. UDPglc_GDPman_dh. 1 hit.
    SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF48179. SSF48179. 1 hit.
    SSF51735. SSF51735. 1 hit.
    SSF52413. SSF52413. 1 hit.
    TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiUDG_STRPY
    AccessioniPrimary (citable) accession number: P0C0F4
    Secondary accession number(s): Q07172
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2005
    Last sequence update: September 13, 2005
    Last modified: November 2, 2016
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.