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P0C0E6

- KITH_VZVO

UniProt

P0C0E6 - KITH_VZVO

Protein

Thymidine kinase

Gene

ORF36

Organism
Varicella-zoster virus (strain Oka vaccine) (HHV-3) (Human herpesvirus 3)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 36 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as nerve cells that do not replicate their genome.By similarity

    Catalytic activityi

    ATP + thymidine = ADP + thymidine 5'-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei48 – 481Proton acceptorSequence Analysis
    Binding sitei66 – 661Substrate
    Binding sitei90 – 901Substrate
    Binding sitei139 – 1391SubstrateBy similarity
    Binding sitei183 – 1831ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi19 – 268ATP

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. thymidine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. TMP biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinase (EC:2.7.1.21)
    Gene namesi
    ORF Names:ORF36
    OrganismiVaricella-zoster virus (strain Oka vaccine) (HHV-3) (Human herpesvirus 3)
    Taxonomic identifieri341980 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000002603: Genome, UP000008504: Genome, UP000008505: Genome, UP000008506: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 341341Thymidine kinasePRO_0000175087Add
    BLAST

    Expressioni

    Keywords - Developmental stagei

    Early protein

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    IntActiP0C0E6. 3 interactions.

    Structurei

    Secondary structure

    1
    341
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi10 – 2314
    Helixi27 – 348
    Turni35 – 384
    Helixi40 – 423
    Beta strandi43 – 464
    Helixi50 – 534
    Helixi61 – 7313
    Helixi79 – 9214
    Helixi94 – 10613
    Beta strandi124 – 1307
    Helixi133 – 1364
    Helixi138 – 1458
    Helixi151 – 1533
    Helixi154 – 1585
    Beta strandi169 – 1746
    Helixi177 – 1837
    Helixi196 – 21823
    Helixi224 – 2274
    Helixi233 – 2397
    Helixi253 – 2553
    Helixi258 – 2614
    Helixi264 – 2663
    Helixi275 – 29016
    Beta strandi292 – 2976
    Helixi302 – 31211
    Helixi313 – 3153
    Beta strandi319 – 3213
    Helixi323 – 33614

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OSNX-ray3.20A/B/C/D1-341[»]
    ProteinModelPortaliP0C0E6.
    SMRiP0C0E6. Positions 8-338.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0C0E6.

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR001889. Herpes_TK.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00693. Herpes_TK. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0C0E6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTDKTDVKM GVLRIYLDGA YGIGKTTAAE EFLHHFAITP NRILLIGEPL    50
    SYWRNLAGED AICGIYGTQT RRLNGDVSPE DAQRLTAHFQ SLFCSPHAIM 100
    HAKISALMDT STSDLVQVNK EPYKIMLSDR HPIASTICFP LSRYLVGDMS 150
    PAALPGLLFT LPAEPPGTNL VVCTVSLPSH LSRVSKRARP GETVNLPFVM 200
    VLRNVYIMLI NTIIFLKTNN WHAGWNTLSF CNDVFKQKLQ KSECIKLREV 250
    PGIEDTLFAV LKLPELCGEF GNILPLWAWG METLSNCLRS MSPFVLSLEQ 300
    TPQHAAQELK TLLPQMTPAN MSSGAWNILK ELVNAVQDNT S 341
    Length:341
    Mass (Da):37,843
    Last modified:September 13, 2005 - v1
    Checksum:iF38ACC39751C076A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36160 Genomic DNA. Translation: AAA45865.1.
    AB009252 Genomic DNA. Translation: BAA23727.2.
    AB009253 Genomic DNA. Translation: BAA23728.2.
    AB097932 Genomic DNA. No translation available.
    AB097933 Genomic DNA. No translation available.
    DQ008354 Genomic DNA. Translation: AAY57648.1.
    DQ008355 Genomic DNA. Translation: AAY57719.1.
    PIRiA28930. KIBEEL.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36160 Genomic DNA. Translation: AAA45865.1 .
    AB009252 Genomic DNA. Translation: BAA23727.2 .
    AB009253 Genomic DNA. Translation: BAA23728.2 .
    AB097932 Genomic DNA. No translation available.
    AB097933 Genomic DNA. No translation available.
    DQ008354 Genomic DNA. Translation: AAY57648.1 .
    DQ008355 Genomic DNA. Translation: AAY57719.1 .
    PIRi A28930. KIBEEL.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1OSN X-ray 3.20 A/B/C/D 1-341 [» ]
    ProteinModelPortali P0C0E6.
    SMRi P0C0E6. Positions 8-338.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P0C0E6. 3 interactions.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P0C0E6.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR001889. Herpes_TK.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00693. Herpes_TK. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular analysis of the pyrimidine deoxyribonucleoside kinase gene of wild-type and acyclovir-resistant strains of varicella-zoster virus."
      Sawyer M.H., Inchauspe G., Biron K.K., Waters D.J., Straus S.E., Ostrove J.M.
      J. Gen. Virol. 69:2585-2593(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Molecular analysis of the thymidine kinase gene of thymidine kinase-deficient mutants of varicella-zoster virus."
      Mori H., Shiraki K., Kato T., Hayakawa Y., Yamanishi K., Takahashi M.
      Intervirology 29:301-310(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Analysis of mutations in the thymidine kinase genes of drug-resistant varicella-zoster virus populations using the polymerase chain reaction."
      Lacey S.F., Suzutani T., Powell K.L., Purifoy D.J., Honess R.W.
      J. Gen. Virol. 72:623-630(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 9883 and YS.
    4. "Comparison of the complete DNA sequences of the Oka varicella vaccine and its parental virus."
      Gomi Y., Sunamachi H., Mori Y., Nagaike K., Takahashi M., Yamanishi K.
      J. Virol. 76:11447-11459(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Isolate Human/Japan/P-Oka/1970 and Oka varicella vaccine Biken (V-Oka-Biken).
    5. "Complete DNA sequences of two oka strain varicella-zoster virus genomes."
      Tillieux S.L., Halsey W.S., Thomas E.S., Voycik J.J., Sathe G.M., Vassilev V.
      J. Virol. 82:11023-11044(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Oka varicella vaccine VarilRix (V-Oka-GSK) and Oka varicella vaccine Varivax V-Oka-Merk).
    6. Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) IN COMPLEX WITH ADP AND BROMOVINYL-DEOXYURIDINE-MONOPHOSPHATE.

    Entry informationi

    Entry nameiKITH_VZVO
    AccessioniPrimary (citable) accession number: P0C0E6
    Secondary accession number(s): O57298, P14344, Q4JQT9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2005
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 36 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Phosphorylates and thereby activates certain drugs like acyclovir (ACV), valacyclovir, and famciclovir to a toxic form, that leads to successful suppression of the infection, while the uninfected cell does not have this ability because it lacks TK. Mutations in thymidine kinase may induce HSV resistance to antiviral therapies in immunocompromised patients. The most frequently observed resistant strains are unable to express TK and are avirulent in animal models of disease. Resistance may be acquired less frequently by selecting variants which no longer recognize ACV or ACV triphosphate as substrates but which retain normal functions By similarity.By similarity

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3