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P0C0B2

- CYSI_BUCAP

UniProt

P0C0B2 - CYSI_BUCAP

Protein

Putative sulfite reductase [NADPH] hemoprotein beta-component

Gene

cysI

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed - Annotation score: 3 out of 5- Protein uncertaini
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate.UniRule annotation

    Catalytic activityi

    H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH.UniRule annotation

    Cofactori

    Binds 1 siroheme per subunit.UniRule annotation
    Binds 1 4Fe-4S cluster per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi430 – 4301Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi436 – 4361Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi475 – 4751Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi479 – 4791Iron (siroheme axial ligand)UniRule annotation
    Metal bindingi479 – 4791Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. heme binding Source: InterPro
    3. metal ion binding Source: UniProtKB-KW
    4. NADP binding Source: InterPro
    5. sulfite reductase (NADPH) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cysteine biosynthetic process Source: UniProtKB-KW
    2. hydrogen sulfide biosynthetic process Source: UniProtKB-HAMAP
    3. sulfate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Cysteine biosynthesis

    Keywords - Ligandi

    4Fe-4S, Heme, Iron, Iron-sulfur, Metal-binding, NADP

    Enzyme and pathway databases

    UniPathwayiUPA00140; UER00207.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative sulfite reductase [NADPH] hemoprotein beta-component (EC:1.8.1.2UniRule annotation)
    Short name:
    SiR-HPUniRule annotation
    Short name:
    SiRHPUniRule annotation
    Gene namesi
    Name:cysIUniRule annotation
    Ordered Locus Names:BUsg_412
    OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
    Taxonomic identifieri198804 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000000416: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. sulfite reductase complex (NADPH) Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 566566Putative sulfite reductase [NADPH] hemoprotein beta-componentPRO_0000199894Add
    BLAST

    Interactioni

    Subunit structurei

    Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliP0C0B2.
    SMRiP0C0B2. Positions 77-566.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.UniRule annotation

    Phylogenomic databases

    OMAiELHQEAY.

    Family and domain databases

    Gene3Di3.90.480.10. 2 hits.
    HAMAPiMF_01540. CysI.
    InterProiIPR011786. CysI.
    IPR005117. NiRdtase/SiRdtase_haem-b_fer.
    IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
    IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
    [Graphical view]
    PfamiPF01077. NIR_SIR. 2 hits.
    PF03460. NIR_SIR_ferr. 2 hits.
    [Graphical view]
    PRINTSiPR00397. SIROHAEM.
    SUPFAMiSSF55124. SSF55124. 2 hits.
    TIGRFAMsiTIGR02041. CysI. 1 hit.
    PROSITEiPS00365. NIR_SIR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0C0B2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKDYKKVFL KNTKEERIKE NSNYLRGTII DDLKDEITNG FTGDNFSLIR    50
    FHGMYQDDRD LRLERNEQKL EPRYAMMLRC RLPRGIIKAK KWLKIDHFAS 100
    KNTLYGTIRL NNLQTFQFHG ILKKTLKDAH KMLNKIGLDS LGTANDVNRN 150
    VLCTSNPMES LIHQQCYEWV SKISNFLLPQ TKAYAEIWLN QKKIATTDQE 200
    PILGKTYLPR KFKTTVVVPP YNDVDLYAND MNFIAITKNN KIVGFNVLIG 250
    GGLSINHGNK NTWPFLAVEL GYITLEKTLS VAESIVTTQR DWGNRTDRKN 300
    AKTRYTIAKV GLSVFKKEVE KRANMTFETI KPYYFISRGD RFGWTKNINN 350
    DWSLTVFIQN GRIYDNDKQL VKSGLLKIAN LHTGNFRLTA NQNIVISEIL 400
    DKNKKKIEEI AISHGLIKKV SSLRENSMAC VSFPTCPLAI AESERILSFF 450
    ITKVENIMLK YGIEKEIIIL RISGCPNGCG RSLLAEIGLI GKSLGRYNLY 500
    IGGNRIGSRI PKIYKENITE QEILIHLDFL IKIWSIERQK KEHFGDFVIR 550
    RNVVKKVVNP IYDFWN 566
    Length:566
    Mass (Da):65,353
    Last modified:September 13, 2005 - v1
    Checksum:iE6F10AF8BC27F19C
    GO

    Sequence cautioni

    The sequence AE013218 differs from that shown. Reason: Frameshift at position 170.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE013218 Genomic DNA. No translation available.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE013218 Genomic DNA. No translation available.

    3D structure databases

    ProteinModelPortali P0C0B2.
    SMRi P0C0B2. Positions 77-566.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    OMAi ELHQEAY.

    Enzyme and pathway databases

    UniPathwayi UPA00140 ; UER00207 .

    Family and domain databases

    Gene3Di 3.90.480.10. 2 hits.
    HAMAPi MF_01540. CysI.
    InterProi IPR011786. CysI.
    IPR005117. NiRdtase/SiRdtase_haem-b_fer.
    IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
    IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
    [Graphical view ]
    Pfami PF01077. NIR_SIR. 2 hits.
    PF03460. NIR_SIR_ferr. 2 hits.
    [Graphical view ]
    PRINTSi PR00397. SIROHAEM.
    SUPFAMi SSF55124. SSF55124. 2 hits.
    TIGRFAMsi TIGR02041. CysI. 1 hit.
    PROSITEi PS00365. NIR_SIR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Sg.

    Entry informationi

    Entry nameiCYSI_BUCAP
    AccessioniPrimary (citable) accession number: P0C0B2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2005
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    Could be the product of a pseudogene.Curated

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Buchnera aphidicola (subsp. Schizaphis graminum)
      Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3