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P0C0B2 (CYSI_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative sulfite reductase [NADPH] hemoprotein beta-component

Short name=SiR-HP
Short name=SiRHP
EC=1.8.1.2
Gene names
Name:cysI
Ordered Locus Names:BUsg_412
OrganismBuchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]
Taxonomic identifier198804 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length566 AA.
Sequence statusComplete.
Protein existenceUncertain

General annotation (Comments)

Function

Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity. HAMAP-Rule MF_01540

Catalytic activity

H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP-Rule MF_01540

Cofactor

Binds 1 siroheme per subunit By similarity. HAMAP-Rule MF_01540

Binds 1 4Fe-4S cluster per subunit By similarity. HAMAP-Rule MF_01540

Pathway

Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1. HAMAP-Rule MF_01540

Subunit structure

Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.

Sequence similarities

Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Caution

Could be the product of a pseudogene.

Sequence caution

The sequence AE013218 differs from that shown. Reason: Frameshift at position 170.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 566566Putative sulfite reductase [NADPH] hemoprotein beta-component HAMAP-Rule MF_01540
PRO_0000199894

Sites

Metal binding4301Iron-sulfur (4Fe-4S) By similarity
Metal binding4361Iron-sulfur (4Fe-4S) By similarity
Metal binding4751Iron-sulfur (4Fe-4S) By similarity
Metal binding4791Iron (siroheme axial ligand) By similarity
Metal binding4791Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
P0C0B2 [UniParc].

Last modified September 13, 2005. Version 1.
Checksum: E6F10AF8BC27F19C

FASTA56665,353
        10         20         30         40         50         60 
MKKDYKKVFL KNTKEERIKE NSNYLRGTII DDLKDEITNG FTGDNFSLIR FHGMYQDDRD 

        70         80         90        100        110        120 
LRLERNEQKL EPRYAMMLRC RLPRGIIKAK KWLKIDHFAS KNTLYGTIRL NNLQTFQFHG 

       130        140        150        160        170        180 
ILKKTLKDAH KMLNKIGLDS LGTANDVNRN VLCTSNPMES LIHQQCYEWV SKISNFLLPQ 

       190        200        210        220        230        240 
TKAYAEIWLN QKKIATTDQE PILGKTYLPR KFKTTVVVPP YNDVDLYAND MNFIAITKNN 

       250        260        270        280        290        300 
KIVGFNVLIG GGLSINHGNK NTWPFLAVEL GYITLEKTLS VAESIVTTQR DWGNRTDRKN 

       310        320        330        340        350        360 
AKTRYTIAKV GLSVFKKEVE KRANMTFETI KPYYFISRGD RFGWTKNINN DWSLTVFIQN 

       370        380        390        400        410        420 
GRIYDNDKQL VKSGLLKIAN LHTGNFRLTA NQNIVISEIL DKNKKKIEEI AISHGLIKKV 

       430        440        450        460        470        480 
SSLRENSMAC VSFPTCPLAI AESERILSFF ITKVENIMLK YGIEKEIIIL RISGCPNGCG 

       490        500        510        520        530        540 
RSLLAEIGLI GKSLGRYNLY IGGNRIGSRI PKIYKENITE QEILIHLDFL IKIWSIERQK 

       550        560 
KEHFGDFVIR RNVVKKVVNP IYDFWN 

« Hide

References

[1]"50 million years of genomic stasis in endosymbiotic bacteria."
Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.
Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Sg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE013218 Genomic DNA. No translation available.

3D structure databases

ProteinModelPortalP0C0B2.
SMRP0C0B2. Positions 77-566.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAELHQEAY.

Enzyme and pathway databases

UniPathwayUPA00140; UER00207.

Family and domain databases

Gene3D3.90.480.10. 2 hits.
HAMAPMF_01540. CysI.
InterProIPR011786. CysI.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
[Graphical view]
PfamPF01077. NIR_SIR. 2 hits.
PF03460. NIR_SIR_ferr. 2 hits.
[Graphical view]
PRINTSPR00397. SIROHAEM.
SUPFAMSSF55124. SSF55124. 2 hits.
TIGRFAMsTIGR02041. CysI. 1 hit.
PROSITEPS00365. NIR_SIR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCYSI_BUCAP
AccessionPrimary (citable) accession number: P0C0B2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: February 19, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Buchnera aphidicola (subsp. Schizaphis graminum)

Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names