Reviewed, UniProtKB/Swiss-Prot P0C0B2 (CYSI_BUCAP)

Names and origin

Protein names

Recommended name:
    Putative sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SIR-HP
      Short name=SIRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	BUsg_412

Organism

Buchnera aphidicola subsp. Schizaphis graminum [Complete proteome] [HAMAP]

Taxonomic identifier

98794 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	566 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Uncertain.

General annotation (Comments)

Function	This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity.
Catalytic activity	H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP MF_01540
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Subunit structure	Alpha(8)-beta4. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.
Caution	Could be the product of a pseudogene.
Sequence caution	The sequence AE013218 differs from that shown. Reason: Frameshift at position 170.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADP or NADPH binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 566	566	Putative sulfite reductase [NADPH] hemoprotein beta-component HAMAP MF_01540		PRO_0000199894
Sites
	Metal binding	430	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	436	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	475	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	479	1	Iron (siroheme axial ligand) By similarity
	Metal binding	479	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0C0B2-1 [UniParc]. Last modified September 13, 2005. Version 1. Checksum: E6F10AF8BC27F19C	FASTA	566	65,353
	10 20 30 40 50 60 MKKDYKKVFL KNTKEERIKE NSNYLRGTII DDLKDEITNG FTGDNFSLIR FHGMYQDDRD 70 80 90 100 110 120 LRLERNEQKL EPRYAMMLRC RLPRGIIKAK KWLKIDHFAS KNTLYGTIRL NNLQTFQFHG 130 140 150 160 170 180 ILKKTLKDAH KMLNKIGLDS LGTANDVNRN VLCTSNPMES LIHQQCYEWV SKISNFLLPQ 190 200 210 220 230 240 TKAYAEIWLN QKKIATTDQE PILGKTYLPR KFKTTVVVPP YNDVDLYAND MNFIAITKNN 250 260 270 280 290 300 KIVGFNVLIG GGLSINHGNK NTWPFLAVEL GYITLEKTLS VAESIVTTQR DWGNRTDRKN 310 320 330 340 350 360 AKTRYTIAKV GLSVFKKEVE KRANMTFETI KPYYFISRGD RFGWTKNINN DWSLTVFIQN 370 380 390 400 410 420 GRIYDNDKQL VKSGLLKIAN LHTGNFRLTA NQNIVISEIL DKNKKKIEEI AISHGLIKKV 430 440 450 460 470 480 SSLRENSMAC VSFPTCPLAI AESERILSFF ITKVENIMLK YGIEKEIIIL RISGCPNGCG 490 500 510 520 530 540 RSLLAEIGLI GKSLGRYNLY IGGNRIGSRI PKIYKENITE QEILIHLDFL IKIWSIERQK 550 560 KEHFGDFVIR RNVVKKVVNP IYDFWN

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013218 Genomic DNA. No translation available.
3D structure databases
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus BUsg_412 in contig AE013218_GR.
KEGG	bas:BUsg412.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0C0B2.
Family and domain databases
HAMAP	MF_01540. [Tree]
InterPro	IPR011786. CysI. IPR006066. Nir_Si_BS. IPR006067. Nir_Sir_4Fe4S. IPR005117. NiRdtase/SiRdtase_haem-b_fer. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_BUCAP

Accession

Primary (citable) accession number: P0C0B2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	September 13, 2005
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)