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P0C0B2

- CYSI_BUCAP

UniProt

P0C0B2 - CYSI_BUCAP

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Protein

Putative sulfite reductase [NADPH] hemoprotein beta-component

Gene

cysI

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed - Annotation score: 3 out of 5- Protein uncertaini

Functioni

Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate.UniRule annotation

Catalytic activityi

H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH.UniRule annotation

Cofactori

Binds 1 siroheme per subunit.UniRule annotation
Binds 1 4Fe-4S cluster per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi430 – 4301Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi436 – 4361Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi475 – 4751Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi479 – 4791Iron (siroheme axial ligand)UniRule annotation
Metal bindingi479 – 4791Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. heme binding Source: InterPro
  3. metal ion binding Source: UniProtKB-KW
  4. NADP binding Source: InterPro
  5. sulfite reductase (NADPH) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cysteine biosynthetic process Source: UniProtKB-KW
  2. hydrogen sulfide biosynthetic process Source: UniProtKB-HAMAP
  3. sulfate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis

Keywords - Ligandi

4Fe-4S, Heme, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00140; UER00207.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative sulfite reductase [NADPH] hemoprotein beta-component (EC:1.8.1.2UniRule annotation)
Short name:
SiR-HPUniRule annotation
Short name:
SiRHPUniRule annotation
Gene namesi
Name:cysIUniRule annotation
Ordered Locus Names:BUsg_412
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000416: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. sulfite reductase complex (NADPH) Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 566566Putative sulfite reductase [NADPH] hemoprotein beta-componentPRO_0000199894Add
BLAST

Interactioni

Subunit structurei

Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP0C0B2.
SMRiP0C0B2. Positions 77-566.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.UniRule annotation

Phylogenomic databases

OMAiELHQEAY.

Family and domain databases

Gene3Di3.90.480.10. 2 hits.
HAMAPiMF_01540. CysI.
InterProiIPR011786. CysI.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
[Graphical view]
PfamiPF01077. NIR_SIR. 2 hits.
PF03460. NIR_SIR_ferr. 2 hits.
[Graphical view]
PRINTSiPR00397. SIROHAEM.
SUPFAMiSSF55124. SSF55124. 2 hits.
TIGRFAMsiTIGR02041. CysI. 1 hit.
PROSITEiPS00365. NIR_SIR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C0B2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKDYKKVFL KNTKEERIKE NSNYLRGTII DDLKDEITNG FTGDNFSLIR
60 70 80 90 100
FHGMYQDDRD LRLERNEQKL EPRYAMMLRC RLPRGIIKAK KWLKIDHFAS
110 120 130 140 150
KNTLYGTIRL NNLQTFQFHG ILKKTLKDAH KMLNKIGLDS LGTANDVNRN
160 170 180 190 200
VLCTSNPMES LIHQQCYEWV SKISNFLLPQ TKAYAEIWLN QKKIATTDQE
210 220 230 240 250
PILGKTYLPR KFKTTVVVPP YNDVDLYAND MNFIAITKNN KIVGFNVLIG
260 270 280 290 300
GGLSINHGNK NTWPFLAVEL GYITLEKTLS VAESIVTTQR DWGNRTDRKN
310 320 330 340 350
AKTRYTIAKV GLSVFKKEVE KRANMTFETI KPYYFISRGD RFGWTKNINN
360 370 380 390 400
DWSLTVFIQN GRIYDNDKQL VKSGLLKIAN LHTGNFRLTA NQNIVISEIL
410 420 430 440 450
DKNKKKIEEI AISHGLIKKV SSLRENSMAC VSFPTCPLAI AESERILSFF
460 470 480 490 500
ITKVENIMLK YGIEKEIIIL RISGCPNGCG RSLLAEIGLI GKSLGRYNLY
510 520 530 540 550
IGGNRIGSRI PKIYKENITE QEILIHLDFL IKIWSIERQK KEHFGDFVIR
560
RNVVKKVVNP IYDFWN
Length:566
Mass (Da):65,353
Last modified:September 13, 2005 - v1
Checksum:iE6F10AF8BC27F19C
GO

Sequence cautioni

The sequence AE013218 differs from that shown. Reason: Frameshift at position 170.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE013218 Genomic DNA. No translation available.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE013218 Genomic DNA. No translation available.

3D structure databases

ProteinModelPortali P0C0B2.
SMRi P0C0B2. Positions 77-566.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

OMAi ELHQEAY.

Enzyme and pathway databases

UniPathwayi UPA00140 ; UER00207 .

Family and domain databases

Gene3Di 3.90.480.10. 2 hits.
HAMAPi MF_01540. CysI.
InterProi IPR011786. CysI.
IPR005117. NiRdtase/SiRdtase_haem-b_fer.
IPR006067. NO2/SO3_Rdtase_4Fe4S_dom.
IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS.
[Graphical view ]
Pfami PF01077. NIR_SIR. 2 hits.
PF03460. NIR_SIR_ferr. 2 hits.
[Graphical view ]
PRINTSi PR00397. SIROHAEM.
SUPFAMi SSF55124. SSF55124. 2 hits.
TIGRFAMsi TIGR02041. CysI. 1 hit.
PROSITEi PS00365. NIR_SIR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sg.

Entry informationi

Entry nameiCYSI_BUCAP
AccessioniPrimary (citable) accession number: P0C0B2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: September 13, 2005
Last modified: October 1, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Could be the product of a pseudogene.Curated

Keywords - Technical termi

Complete proteome

Documents

  1. Buchnera aphidicola (subsp. Schizaphis graminum)
    Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3