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Protein

Vacuolar protein-sorting-associated protein 25

Gene

Vps25

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex (By similarity). The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

ReactomeiR-RNO-917729. Endosomal Sorting Complex Required For Transport (ESCRT).

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein-sorting-associated protein 25
Alternative name(s):
ELL-associated protein of 20 kDa
ESCRT-II complex subunit VPS25
Gene namesi
Name:Vps25
Synonyms:Eap20
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1584685. Vps25.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002152181 – 176Vacuolar protein-sorting-associated protein 25Add BLAST176

Proteomic databases

PaxDbiP0C0A1.
PRIDEiP0C0A1.

Expressioni

Gene expression databases

BgeeiENSRNOG00000051179.
GenevisibleiP0C0A1. RN.

Interactioni

Subunit structurei

Component of a complex at least composed of ELL, SNF8/EAP30, VPS25/EAP20 and VPS36/EAP45 (By similarity). Component of the endosomal sorting complex required for transport II (ESCRT-II), composed of SNF8, VPS36 and 2 copies of VPS25. Interacts with CFTR; the interaction requires misfolded CFTR. Interacts (via C-terminal half) with the ESCRT-III subunit CHMP6 (via N-terminal half) (By similarity).By similarity

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliP0C0A1.
SMRiP0C0A1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the VPS25 family.Curated

Phylogenomic databases

eggNOGiKOG0584. Eukaryota.
KOG4068. Eukaryota.
ENOG410XQWZ. LUCA.
GeneTreeiENSGT00390000014892.
HOGENOMiHOG000191978.
HOVERGENiHBG080015.
KOiK12189.
OMAiPPFFTIQ.
OrthoDBiEOG091G0PIU.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.10.570. 1 hit.
InterProiView protein in InterPro
IPR008570. ESCRT-II_cplx_vps25-sub.
IPR014041. ESCRT-II_cplx_Vps25-sub_N.
IPR036388. WH-like_DNA-bd_sf.
IPR036390. WH_DNA-bd_sf.
PANTHERiPTHR13149. PTHR13149. 1 hit.
PfamiView protein in Pfam
PF05871. ESCRT-II. 1 hit.
SUPFAMiSSF46785. SSF46785. 2 hits.

Sequencei

Sequence statusi: Complete.

P0C0A1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMSFEWPWQ YRFPPFFTLQ PNVDTRQKQL AAWCSLVLSF CRLHKQSSMT
60 70 80 90 100
VMEAQESPLF NNVKLQRKLP VESIQIVLEE LRKKGNLEWL DKNKSSFLIM
110 120 130 140 150
WRRPEEWGKL IYQWVSRSGQ NNSVFTLYEL TSGEDTEEEE FHGLDEATLL
160 170
RALQALQQEH KAEIITVSDG RGVKFF
Length:176
Mass (Da):20,762
Last modified:August 30, 2005 - v1
Checksum:iAD4E3A4617BA4108
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53E → Q AA sequence (PubMed:11278625).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03074956 Genomic DNA. No translation available.
CK471265 mRNA. No translation available.
RefSeqiNP_001166922.1. NM_001173451.1.
UniGeneiRn.6985.

Genome annotation databases

EnsembliENSRNOT00000043754; ENSRNOP00000044397; ENSRNOG00000051179.
GeneIDi681059.
KEGGirno:681059.

Similar proteinsi

Entry informationi

Entry nameiVPS25_RAT
AccessioniPrimary (citable) accession number: P0C0A1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: August 30, 2005
Last modified: November 22, 2017
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families