Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nucleoid occlusion factor SlmA

Gene

slmA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for nucleoid occlusion (NO) phenomenon, which prevents Z-ring formation and cell division over the nucleoid. Acts as a DNA-associated cell division inhibitor that binds simultaneously chromosomal DNA and FtsZ, and disrupts the assembly of FtsZ polymers. SlmA-DNA-binding sequences (SBS) are dispersed on non-Ter regions of the chromosome, preventing FtsZ polymerization at these regions.UniRule annotation3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi33 – 5220H-T-H motifUniRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: EcoliWiki
  • identical protein binding Source: IntAct
  • sequence-specific DNA binding Source: EcoCyc

GO - Biological processi

  • barrier septum site selection Source: EcoliWiki
  • negative regulation of barrier septum assembly Source: EcoliWiki
  • negative regulation of protein polymerization Source: EcoCyc
  • positive regulation of GTPase activity Source: EcoliWiki
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11191-MONOMER.
ECOL316407:JW5641-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoid occlusion factor SlmAUniRule annotation
Alternative name(s):
Protein Ttk
Synthetically lethal with a defective Min system protein A
Gene namesi
Name:slmAUniRule annotation
Synonyms:ttk, yicB
Ordered Locus Names:b3641, JW5641
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11191. slmA.

Subcellular locationi

  • Cytoplasmnucleoid UniRule annotation1 Publication

GO - Cellular componenti

  • bacterial nucleoid Source: EcoCyc
  • cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi33 – 331T → A: Does not associate with DNA, but can inhibit division when overproduced. 1 Publication
Mutagenesisi73 – 731R → D: Loss of activity. Binds DNA, but does not associate with FtsZ polymers. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 198198Nucleoid occlusion factor SlmAPRO_0000198966Add
BLAST

Proteomic databases

PaxDbiP0C093.
PRIDEiP0C093.

Interactioni

Subunit structurei

Homodimer. Interacts with FtsZ.UniRule annotation3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-8520580,EBI-8520580

Protein-protein interaction databases

IntActiP0C093. 1 interaction.
MINTiMINT-8092383.
STRINGi511145.b3641.

Structurei

Secondary structure

1
198
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi12 – 2413Combined sources
Helixi34 – 407Combined sources
Helixi45 – 495Combined sources
Helixi55 – 8026Combined sources
Helixi84 – 10118Combined sources
Helixi103 – 1097Combined sources
Helixi122 – 14120Combined sources
Turni142 – 1465Combined sources
Helixi155 – 17420Combined sources
Turni175 – 1784Combined sources
Turni182 – 1854Combined sources
Helixi186 – 1949Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3NXCX-ray2.50A1-198[»]
ProteinModelPortaliP0C093.
SMRiP0C093. Positions 9-198.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 7061HTH tetR-typeUniRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili117 – 14428UniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the nucleoid occlusion factor SlmA family.UniRule annotation
Contains 1 HTH tetR-type DNA-binding domain.UniRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG1309.
HOGENOMiHOG000266053.
InParanoidiP0C093.
KOiK05501.
OMAiEKDTSAR.
OrthoDBiEOG6G7R3C.
PhylomeDBiP0C093.

Family and domain databases

Gene3Di1.10.357.10. 1 hit.
HAMAPiMF_01839. NO_factor_SlmA.
InterProiIPR023772. DNA-bd_HTH_TetR-type_CS.
IPR009057. Homeodomain-like.
IPR001647. HTH_TetR.
IPR023769. NO_SlmA.
IPR015893. Tet_transcr_reg_TetR-like_C.
IPR011075. Tet_transcr_reg_TetR-rel_C.
[Graphical view]
PfamiPF00440. TetR_N. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF48498. SSF48498. 1 hit.
PROSITEiPS01081. HTH_TETR_1. 1 hit.
PS50977. HTH_TETR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C093-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEKQTAKRN RREEILQSLA LMLESSDGSQ RITTAKLAAS VGVSEAALYR
60 70 80 90 100
HFPSKTRMFD SLIEFIEDSL ITRINLILKD EKDTTARLRL IVLLLLGFGE
110 120 130 140 150
RNPGLTRILT GHALMFEQDR LQGRINQLFE RIEAQLRQVL REKRMREGEG
160 170 180 190
YTTDETLLAS QILAFCEGML SRFVRSEFKY RPTDDFDARW PLIAAQLQ
Length:198
Mass (Da):22,836
Last modified:August 16, 2005 - v1
Checksum:iC797B0E2875B0E39
GO

Sequence cautioni

The sequence AAA61994.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti196 – 1983QLQ → SCSNMTPDDFSSGEFL in CAA25860 (PubMed:6139280).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01714 Genomic DNA. Translation: CAA25860.1.
V01578 Genomic DNA. Translation: CAA24898.1.
V01498 Genomic DNA. No translation available.
L10328 Genomic DNA. Translation: AAA61994.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76665.2.
AP009048 Genomic DNA. Translation: BAE77651.1.
RefSeqiNP_418098.4. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC76665; AAC76665; b3641.
BAE77651; BAE77651; BAE77651.
GeneIDi948158.
KEGGiecj:Y75_p3533.
eco:b3641.
PATRICi32122769. VBIEscCol129921_3761.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01714 Genomic DNA. Translation: CAA25860.1.
V01578 Genomic DNA. Translation: CAA24898.1.
V01498 Genomic DNA. No translation available.
L10328 Genomic DNA. Translation: AAA61994.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76665.2.
AP009048 Genomic DNA. Translation: BAE77651.1.
RefSeqiNP_418098.4. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3NXCX-ray2.50A1-198[»]
ProteinModelPortaliP0C093.
SMRiP0C093. Positions 9-198.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP0C093. 1 interaction.
MINTiMINT-8092383.
STRINGi511145.b3641.

Proteomic databases

PaxDbiP0C093.
PRIDEiP0C093.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76665; AAC76665; b3641.
BAE77651; BAE77651; BAE77651.
GeneIDi948158.
KEGGiecj:Y75_p3533.
eco:b3641.
PATRICi32122769. VBIEscCol129921_3761.

Organism-specific databases

EchoBASEiEB1177.
EcoGeneiEG11191. slmA.

Phylogenomic databases

eggNOGiCOG1309.
HOGENOMiHOG000266053.
InParanoidiP0C093.
KOiK05501.
OMAiEKDTSAR.
OrthoDBiEOG6G7R3C.
PhylomeDBiP0C093.

Enzyme and pathway databases

BioCyciEcoCyc:EG11191-MONOMER.
ECOL316407:JW5641-MONOMER.

Miscellaneous databases

PROiP0C093.

Family and domain databases

Gene3Di1.10.357.10. 1 hit.
HAMAPiMF_01839. NO_factor_SlmA.
InterProiIPR023772. DNA-bd_HTH_TetR-type_CS.
IPR009057. Homeodomain-like.
IPR001647. HTH_TetR.
IPR023769. NO_SlmA.
IPR015893. Tet_transcr_reg_TetR-like_C.
IPR011075. Tet_transcr_reg_TetR-rel_C.
[Graphical view]
PfamiPF00440. TetR_N. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF48498. SSF48498. 1 hit.
PROSITEiPS01081. HTH_TETR_1. 1 hit.
PS50977. HTH_TETR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the structural gene for dUTPase of Escherichia coli K-12."
    Lundberg L.G., Thoresson H.-O., Karlstroem O.H., Nyman P.O.
    EMBO J. 2:967-971(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
    Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
    Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Lethality of a dut (deoxyuridine triphosphatase) mutation in Escherichia coli."
    El-Hajj H.H., Zhang H., Weiss B.
    J. Bacteriol. 170:1069-1075(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE NAME.
  6. "SlmA, a nucleoid-associated, ftsZ binding protein required for blocking septal ring assembly over chromosomes in E. coli."
    Bernhardt T.G., de Boer P.A.J.
    Mol. Cell 18:555-564(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH FTSZ.
  7. "Nucleoid occlusion factor SlmA is a DNA-activated FtsZ polymerization antagonist."
    Cho H., McManus H.R., Dove S.L., Bernhardt T.G.
    Proc. Natl. Acad. Sci. U.S.A. 108:3773-3778(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH FTSZ, REGULATION OF ACTIVITY, MUTAGENESIS OF THR-33 AND ARG-73.
  8. "Molecular mechanism by which the nucleoid occlusion factor, SlmA, keeps cytokinesis in check."
    Tonthat N.K., Arold S.T., Pickering B.F., Van Dyke M.W., Liang S., Lu Y., Beuria T.K., Margolin W., Schumacher M.A.
    EMBO J. 30:154-164(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), FUNCTION, DNA-BINDING, SUBUNIT, INTERACTION WITH FTSZ.

Entry informationi

Entry nameiSLMA_ECOLI
AccessioniPrimary (citable) accession number: P0C093
Secondary accession number(s): P06969, Q2M7V5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: August 16, 2005
Last modified: June 24, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Inhibitory activity is enhanced by sequence-specific DNA binding.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.