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P0C093 (SLMA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nucleoid occlusion factor SlmA
Alternative name(s):
Protein Ttk
Synthetically lethal with a defective Min system protein A
Gene names
Name:slmA
Synonyms:ttk, yicB
Ordered Locus Names:b3641, JW5641
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length198 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for nucleoid occlusion (NO) phenomenon, which prevents Z-ring formation and cell division over the nucleoid. Acts as a DNA-associated cell division inhibitor that binds simultaneously chromosomal DNA and FtsZ, and disrupts the assembly of FtsZ polymers. SlmA-DNA-binding sequences (SBS) are dispersed on non-Ter regions of the chromosome, preventing FtsZ polymerization at these regions. Ref.6 Ref.7 Ref.8

Subunit structure

Homodimer. Interacts with FtsZ. Ref.6 Ref.7 Ref.8

Subcellular location

Cytoplasmnucleoid Ref.6.

Miscellaneous

Inhibitory activity is enhanced by sequence-specific DNA binding.

Sequence similarities

Belongs to the nucleoid occlusion factor SlmA family.

Contains 1 HTH tetR-type DNA-binding domain.

Sequence caution

The sequence AAA61994.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself2EBI-8520580,EBI-8520580

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 198198Nucleoid occlusion factor SlmA HAMAP-Rule MF_01839
PRO_0000198966

Regions

Domain10 – 7061HTH tetR-type
DNA binding33 – 5220H-T-H motif By similarity
Coiled coil117 – 14428 Potential

Experimental info

Mutagenesis331T → A: Does not associate with DNA, but can inhibit division when overproduced. Ref.7
Mutagenesis731R → D: Loss of activity. Binds DNA, but does not associate with FtsZ polymers. Ref.7
Sequence conflict196 – 1983QLQ → SCSNMTPDDFSSGEFL in CAA25860. Ref.1

Secondary structure

...................... 198
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0C093 [UniParc].

Last modified August 16, 2005. Version 1.
Checksum: C797B0E2875B0E39

FASTA19822,836
        10         20         30         40         50         60 
MAEKQTAKRN RREEILQSLA LMLESSDGSQ RITTAKLAAS VGVSEAALYR HFPSKTRMFD 

        70         80         90        100        110        120 
SLIEFIEDSL ITRINLILKD EKDTTARLRL IVLLLLGFGE RNPGLTRILT GHALMFEQDR 

       130        140        150        160        170        180 
LQGRINQLFE RIEAQLRQVL REKRMREGEG YTTDETLLAS QILAFCEGML SRFVRSEFKY 

       190 
RPTDDFDARW PLIAAQLQ 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the structural gene for dUTPase of Escherichia coli K-12."
Lundberg L.G., Thoresson H.-O., Karlstroem O.H., Nyman P.O.
EMBO J. 2:967-971(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Lethality of a dut (deoxyuridine triphosphatase) mutation in Escherichia coli."
El-Hajj H.H., Zhang H., Weiss B.
J. Bacteriol. 170:1069-1075(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE NAME.
[6]"SlmA, a nucleoid-associated, ftsZ binding protein required for blocking septal ring assembly over chromosomes in E. coli."
Bernhardt T.G., de Boer P.A.J.
Mol. Cell 18:555-564(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH FTSZ.
[7]"Nucleoid occlusion factor SlmA is a DNA-activated FtsZ polymerization antagonist."
Cho H., McManus H.R., Dove S.L., Bernhardt T.G.
Proc. Natl. Acad. Sci. U.S.A. 108:3773-3778(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH FTSZ, REGULATION OF ACTIVITY, MUTAGENESIS OF THR-33 AND ARG-73.
[8]"Molecular mechanism by which the nucleoid occlusion factor, SlmA, keeps cytokinesis in check."
Tonthat N.K., Arold S.T., Pickering B.F., Van Dyke M.W., Liang S., Lu Y., Beuria T.K., Margolin W., Schumacher M.A.
EMBO J. 30:154-164(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), FUNCTION, DNA-BINDING, SUBUNIT, INTERACTION WITH FTSZ.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X01714 Genomic DNA. Translation: CAA25860.1.
V01578 Genomic DNA. Translation: CAA24898.1.
V01498 Genomic DNA. No translation available.
L10328 Genomic DNA. Translation: AAA61994.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76665.2.
AP009048 Genomic DNA. Translation: BAE77651.1.
RefSeqNP_418098.4. NC_000913.3.
YP_491792.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3NXCX-ray2.50A1-198[»]
ProteinModelPortalP0C093.
SMRP0C093. Positions 9-198.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP0C093. 1 interaction.
MINTMINT-8092383.
STRING511145.b3641.

Proteomic databases

PaxDbP0C093.
PRIDEP0C093.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76665; AAC76665; b3641.
BAE77651; BAE77651; BAE77651.
GeneID12930420.
948158.
KEGGecj:Y75_p3533.
eco:b3641.
PATRIC32122769. VBIEscCol129921_3761.

Organism-specific databases

EchoBASEEB1177.
EcoGeneEG11191. slmA.

Phylogenomic databases

eggNOGCOG1309.
HOGENOMHOG000266053.
KOK05501.
OMAEKDTSAR.
OrthoDBEOG6G7R3C.
PhylomeDBP0C093.

Enzyme and pathway databases

BioCycEcoCyc:EG11191-MONOMER.
ECOL316407:JW5641-MONOMER.

Gene expression databases

GenevestigatorP0C093.

Family and domain databases

Gene3D1.10.357.10. 1 hit.
HAMAPMF_01839. NO_factor_SlmA.
InterProIPR023772. DNA-bd_HTH_TetR-type_CS.
IPR009057. Homeodomain-like.
IPR001647. HTH_TetR.
IPR023769. NO_SlmA.
IPR015893. Tet_transcr_reg_TetR-like_C.
IPR011075. Tet_transcr_reg_TetR-rel_C.
[Graphical view]
PfamPF00440. TetR_N. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF48498. SSF48498. 1 hit.
PROSITEPS01081. HTH_TETR_1. 1 hit.
PS50977. HTH_TETR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP0C093.

Entry information

Entry nameSLMA_ECOLI
AccessionPrimary (citable) accession number: P0C093
Secondary accession number(s): P06969, Q2M7V5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: August 16, 2005
Last modified: June 11, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene