Toxin relE - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P0C077 (RELE_ECOLI)

Last modified June 16, 2009. Version 31. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
Toxin relE

Gene names

Name:	relE
Ordered Locus Names:	b1563, JW1555

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	95 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibits translation during amino acid starvation. Acts by cleaving mRNA with high codon specificity in the ribosomal A site. UGA is cleaved with fast rate while UAA and UGA are cleaved with intermediate and slow rate, respectively. mRNA cleavage can also occur in the ribosomal E site after peptide release from peptidyl-tRNA in the P site as well as on free 30S subunit. Also acts with relB as a corepressor of relBE transcription. Overproduction of relE has been shown to sharply increase persisters (cells that neither grow or die in presence of bactericidal agent and are largely responsible for high levels of biofilm tolerance to antimicrobials). Ref.5 Ref.6 Ref.7 Ref.8
Subunit structure	Part of the toxin/antitoxin system relE/relB. Ref.6
Induction	By amino acid starvation.

Ontologies

Keywords
Biological process	Stress response Transcription Transcription regulation
Molecular function	Repressor Toxin
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

95

Toxin relE

PRO_0000097245

Sequences

Sequence

Length

Mass (Da)

Tools

P0C077-1 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: F516B2E7A437CCEC
Show »

95

11,225

        10         20         30         40         50         60 
MAYFLDFDER ALKEWRKLGS TVREQLKKKL VEVLESPRIE ANKLRGMPDC YKIKLRSSGY 

        70         80         90 
RLVYQVIDEK VVVFVISVGK RERSEVYSEA VKRIL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the relB transcription unit from Escherichia coli and identification of the relB gene." Bech F.W., Joergensen S.T., Diderichsen B., Karlstroem O.H. EMBO J. 4:1059-1066(1985) [PubMed: 2990907] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The Escherichia coli relBE genes belong to a new toxin-antitoxin gene family." Gotfredsen M., Gerdes K. Mol. Microbiol. 29:1065-1076(1998) [PubMed: 9767574] [Abstract] Cited for: FUNCTION.
[6]	"Purification of the RelB and RelE proteins of Escherichia coli: RelE binds to RelB and to ribosomes." Galvani C., Terry J., Ishiguro E.E. J. Bacteriol. 183:2700-2703(2001) [PubMed: 11274135] [Abstract] Cited for: FUNCTION, SUBUNIT.
[7]	"RelE, a global inhibitor of translation, is activated during nutritional stress." Christensen S.K., Mikkelsen M., Pedersen K., Gerdes K. Proc. Natl. Acad. Sci. U.S.A. 98:14328-14333(2001) [PubMed: 11717402] [Abstract] Cited for: FUNCTION.
[8]	"The bacterial toxin RelE displays codon-specific cleavage of mRNAs in the ribosomal A site." Pedersen K., Zavialov A.V., Pavlov M.Y., Elf J., Gerdes K., Ehrenberg M. Cell 112:131-140(2003) [PubMed: 12526800] [Abstract] Cited for: FUNCTION.
[9]	"Specialized persister cells and the mechanism of multidrug tolerance in Escherichia coli." Keren I., Shah D., Spoering A., Kaldalu N., Lewis K. J. Bacteriol. 186:8172-8180(2004) [PubMed: 15576765] [Abstract] Cited for: RELATION WITH PERSISTERS.

Cross-references

Sequence databases

X02405 Genomic DNA. Translation: CAA26251.1.
U00096 Genomic DNA. Translation: AAC74636.1.
AP009048 Genomic DNA. Translation: BAA15262.1.

PIR

QQECR1. B22830.

RefSeq

AP_002185.1.
NP_416081.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KC8	NMR	-	A	1-95	[»]
2KC9	NMR	-	A	1-95	[»]

ModBase

Genome annotation databases

GeneID

GenomeReviews

Gene locus JW1555 in contig AP009048_GR.
Gene locus b1563 in contig U00096_GR.

KEGG

ecj:JW1555.
eco:b1563.

Organism-specific databases

EchoBASE

EcoGene

EG11131. relE.

CMR

Phylogenomic databases

HOGENOM

OMA

P0C077. PRVEANR.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11131-MON.

Family and domain databases

InterPro

IPR007712. Plasmid_stabil.
IPR012753. RelE_StbE.
[Graphical view]

Pfam

PF05016. Plasmid_stabil. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02385. RelE_StbE. 1 hit.

ProtoNet

Entry information

Entry name

RELE_ECOLI

Accession

Primary (citable) accession number: P0C077
Secondary accession number(s): P07008

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents