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Protein

Membrane-bound lytic murein transglycosylase C

Gene

mltC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division.UniRule annotation

Catalytic activityi

Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.UniRule annotation

GO - Molecular functioni

GO - Biological processi

  • cellular response to antibiotic Source: EcoCyc
  • cellular response to oxidative stress Source: EcoCyc
  • cell wall macromolecule catabolic process Source: UniProtKB-HAMAP
  • cell wall organization Source: UniProtKB-KW
  • peptidoglycan metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciEcoCyc:G7533-MONOMER.
ECOL316407:JW5481-MONOMER.
MetaCyc:G7533-MONOMER.

Protein family/group databases

CAZyiGH23. Glycoside Hydrolase Family 23.

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane-bound lytic murein transglycosylase CUniRule annotation (EC:4.2.2.n1UniRule annotation)
Alternative name(s):
Murein lyase CUniRule annotation
Gene namesi
Name:mltCUniRule annotation
Synonyms:yggZ
Ordered Locus Names:b2963, JW5481
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12986. mltC.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16UniRule annotationAdd BLAST16
ChainiPRO_000003278517 – 359Membrane-bound lytic murein transglycosylase CAdd BLAST343

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi17N-palmitoyl cysteineUniRule annotation1
Lipidationi17S-diacylglycerol cysteineUniRule annotation1

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiP0C066.
PRIDEiP0C066.

Expressioni

Inductioni

By SoxS.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4262361. 451 interactors.
STRINGi511145.b2963.

Structurei

Secondary structure

1359
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi36 – 52Combined sources17
Helixi54 – 56Combined sources3
Beta strandi62 – 69Combined sources8
Turni70 – 73Combined sources4
Beta strandi74 – 79Combined sources6
Turni80 – 83Combined sources4
Beta strandi84 – 89Combined sources6
Helixi95 – 108Combined sources14
Helixi113 – 115Combined sources3
Beta strandi120 – 122Combined sources3
Beta strandi129 – 131Combined sources3
Beta strandi140 – 142Combined sources3
Helixi148 – 161Combined sources14
Beta strandi164 – 167Combined sources4
Beta strandi169 – 179Combined sources11
Helixi184 – 190Combined sources7
Helixi193 – 203Combined sources11
Helixi207 – 218Combined sources12
Turni232 – 235Combined sources4
Turni238 – 240Combined sources3
Helixi241 – 247Combined sources7
Turni248 – 250Combined sources3
Helixi257 – 260Combined sources4
Helixi263 – 279Combined sources17
Turni280 – 284Combined sources5
Helixi288 – 301Combined sources14
Helixi303 – 307Combined sources5
Turni308 – 310Combined sources3
Helixi314 – 321Combined sources8
Helixi326 – 335Combined sources10
Helixi340 – 357Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4C5FX-ray2.34A/B20-359[»]
4CFOX-ray2.90A/B20-359[»]
4CFPX-ray2.15A/B20-359[»]
4CHXX-ray2.45A/B20-359[»]
ProteinModelPortaliP0C066.
SMRiP0C066.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the transglycosylase Slt family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4106S48. Bacteria.
COG0741. LUCA.
HOGENOMiHOG000283572.
KOiK08306.
OMAiAIMQIES.

Family and domain databases

HAMAPiMF_01616. MltC. 1 hit.
InterProiIPR023346. Lysozyme-like_dom.
IPR023664. Murein_transglycosylaseC.
IPR024570. Murein_transglycosylaseC_N.
IPR000189. Transglyc_AS.
IPR008258. Transglycosylase_SLT_dom_1.
[Graphical view]
PfamiPF11873. DUF3393. 1 hit.
PF01464. SLT. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
PS00922. TRANSGLYCOSYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C066-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKYLALALI APLLISCSTT KKGDTYNEAW VKDTNGFDIL MGQFAHNIEN
60 70 80 90 100
IWGFKEVVIA GPKDYVKYTD QYQTRSHINF DDGTITIETI AGTEPAAHLR
110 120 130 140 150
RAIIKTLLMG DDPSSVDLYS DVDDITISKE PFLYGQVVDN TGQPIRWEGR
160 170 180 190 200
ASNFADYLLK NRLKSRSNGL RIIYSVTINM VPNHLDKRAH KYLGMVRQAS
210 220 230 240 250
RKYGVDESLI LAIMQTESSF NPYAVSRSDA LGLMQVVQHT AGKDVFRSQG
260 270 280 290 300
KSGTPSRSFL FDPASNIDTG TAYLAMLNNV YLGGIDNPTS RRYAVITAYN
310 320 330 340 350
GGAGSVLRVF SNDKIQAANI INTMTPGDVY QTLTTRHPSA ESRRYLYKVN

TAQKSYRRR
Length:359
Mass (Da):40,113
Last modified:July 19, 2005 - v1
Checksum:i618F0FCC9C41969C
GO

Sequence cautioni

The sequence AAA69130 differs from that shown. Reason: Frameshift at position 166.Curated
The sequence AAA69130 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAA69131 differs from that shown. Reason: Frameshift at position 166.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28377 Genomic DNA. Translation: AAA69130.1. Sequence problems.
U28377 Genomic DNA. Translation: AAA69131.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76000.2.
AP009048 Genomic DNA. Translation: BAE77026.1.
U59902 Genomic DNA. Translation: AAC44296.1.
PIRiB65082.
RefSeqiNP_417438.2. NC_000913.3.
WP_000760323.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76000; AAC76000; b2963.
BAE77026; BAE77026; BAE77026.
GeneIDi945428.
KEGGiecj:JW5481.
eco:b2963.
PATRICi32121340. VBIEscCol129921_3058.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28377 Genomic DNA. Translation: AAA69130.1. Sequence problems.
U28377 Genomic DNA. Translation: AAA69131.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76000.2.
AP009048 Genomic DNA. Translation: BAE77026.1.
U59902 Genomic DNA. Translation: AAC44296.1.
PIRiB65082.
RefSeqiNP_417438.2. NC_000913.3.
WP_000760323.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4C5FX-ray2.34A/B20-359[»]
4CFOX-ray2.90A/B20-359[»]
4CFPX-ray2.15A/B20-359[»]
4CHXX-ray2.45A/B20-359[»]
ProteinModelPortaliP0C066.
SMRiP0C066.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262361. 451 interactors.
STRINGi511145.b2963.

Protein family/group databases

CAZyiGH23. Glycoside Hydrolase Family 23.

Proteomic databases

PaxDbiP0C066.
PRIDEiP0C066.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76000; AAC76000; b2963.
BAE77026; BAE77026; BAE77026.
GeneIDi945428.
KEGGiecj:JW5481.
eco:b2963.
PATRICi32121340. VBIEscCol129921_3058.

Organism-specific databases

EchoBASEiEB2810.
EcoGeneiEG12986. mltC.

Phylogenomic databases

eggNOGiENOG4106S48. Bacteria.
COG0741. LUCA.
HOGENOMiHOG000283572.
KOiK08306.
OMAiAIMQIES.

Enzyme and pathway databases

BioCyciEcoCyc:G7533-MONOMER.
ECOL316407:JW5481-MONOMER.
MetaCyc:G7533-MONOMER.

Miscellaneous databases

PROiP0C066.

Family and domain databases

HAMAPiMF_01616. MltC. 1 hit.
InterProiIPR023346. Lysozyme-like_dom.
IPR023664. Murein_transglycosylaseC.
IPR024570. Murein_transglycosylaseC_N.
IPR000189. Transglyc_AS.
IPR008258. Transglycosylase_SLT_dom_1.
[Graphical view]
PfamiPF11873. DUF3393. 1 hit.
PF01464. SLT. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
PS00922. TRANSGLYCOSYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMLTC_ECOLI
AccessioniPrimary (citable) accession number: P0C066
Secondary accession number(s): P52066
, P71274, P76651, P76652, Q2M9N0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: November 30, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.