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Protein

Gramicidin S synthase 2

Gene

grsB

Organism
Brevibacillus brevis (Bacillus brevis)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein is a multifunctional enzyme, able to activate and polymerize the amino acids Pro, Val, Orn and Leu. Activation sites for these AA consist of individual domains.

Cofactori

pantetheine 4'-phosphateNote: Binds 4 phosphopantetheines covalently.

Pathwayi: gramicidin S biosynthesis

This protein is involved in the pathway gramicidin S biosynthesis, which is part of Antibiotic biosynthesis.
View all proteins of this organism that are known to be involved in the pathway gramicidin S biosynthesis and in Antibiotic biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Ligase
Biological processAntibiotic biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00102.

Protein family/group databases

ESTHERibacbr-grsb. Thioesterase.

Names & Taxonomyi

Protein namesi
Recommended name:
Gramicidin S synthase 2
Alternative name(s):
Gramicidin S synthase II
Including the following 4 domains:
ATP-dependent proline adenylase
Short name:
ProA
Alternative name(s):
Proline activase
ATP-dependent valine adenylase
Short name:
ValA
Alternative name(s):
Valine activase
ATP-dependent ornithine adenylase
Short name:
OrnA
Alternative name(s):
Ornithine activase
ATP-dependent leucine adenylase
Short name:
LeuA
Alternative name(s):
Leucine activase
Gene namesi
Name:grsB
Synonyms:grs2
OrganismiBrevibacillus brevis (Bacillus brevis)
Taxonomic identifieri1393 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeBrevibacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001930882 – 4450Gramicidin S synthase 2Add BLAST4449

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1006O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1
Modified residuei2041O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1
Modified residuei3086O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1
Modified residuei4124O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Interactioni

Subunit structurei

Large multienzyme complex of GrsA and GrsB.

Structurei

3D structure databases

ProteinModelPortaliP0C064.
SMRiP0C064.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini971 – 1046Carrier 1PROSITE-ProRule annotationAdd BLAST76
Domaini2006 – 2081Carrier 2PROSITE-ProRule annotationAdd BLAST76
Domaini3051 – 3126Carrier 3PROSITE-ProRule annotationAdd BLAST76
Domaini4089 – 4164Carrier 4PROSITE-ProRule annotationAdd BLAST76

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni467 – 1044Domain 1 (proline-activating)By similarityAdd BLAST578
Regioni1521 – 2080Domain 2 (valine-activating)By similarityAdd BLAST560
Regioni2538 – 3134Domain 3 (ornithine-activating)By similarityAdd BLAST597
Regioni3590 – 4172Domain 4 (leucine-activating)By similarityAdd BLAST583

Domaini

Consists of four modules, and harbors a putative thioesterase domain at its C-terminal end. Each module incorporates one amino acid into the peptide product and can be further subdivided into domains responsible for substrate adenylation, thiolation, condensation (not for the initiation module), and epimerization (optional), and N methylation (optional).

Sequence similaritiesi

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di1.10.1200.10. 4 hits.
3.40.50.1820. 1 hit.
InterProiView protein in InterPro
IPR010071. AA_adenyl_domain.
IPR029058. AB_hydrolase.
IPR036736. ACP-like_sf.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR001242. Condensatn.
IPR020806. PKS_PP-bd.
IPR009081. PP-bd_ACP.
IPR006162. Ppantetheine_attach_site.
IPR001031. Thioesterase.
PfamiView protein in Pfam
PF00501. AMP-binding. 4 hits.
PF13193. AMP-binding_C. 4 hits.
PF00668. Condensation. 4 hits.
PF00550. PP-binding. 4 hits.
PF00975. Thioesterase. 1 hit.
SMARTiView protein in SMART
SM00823. PKS_PP. 4 hits.
SUPFAMiSSF47336. SSF47336. 4 hits.
SSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01733. AA-adenyl-dom. 4 hits.
PROSITEiView protein in PROSITE
PS00455. AMP_BINDING. 4 hits.
PS50075. CARRIER. 4 hits.
PS00012. PHOSPHOPANTETHEINE. 4 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0C064-1 [UniParc]FASTAAdd to basket

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        10         20         30         40         50
MSTFKKEHVQ DMYRLSPMQE GMLFHALLDK DKNAHLVQMS IAIEGIVDVE
60 70 80 90 100
LLSESLNILI DRYDVFRTTF LHEKIKQPLQ VVLKERPVQL QFKDISSLDE
110 120 130 140 150
EKREQAIEQY KYQDGETVFD LTRDPLMRVA IFQTGKVNYQ MIWSFHHILM
160 170 180 190 200
DGWCFNIIFN DLFNIYLSLK EKKPLQLEAV QPYKQFIKWL EKQDKQEALR
210 220 230 240 250
YWKEHLMNYD QSVTLPKKKA AINNTTYEPA QFRFAFDKVL TQQLLRIANQ
260 270 280 290 300
SQVTLNIVFQ TIWGIVLQKY NSTNDVVYGS VVSGRPSEIS GIEKMVGLFI
310 320 330 340 350
NTLPLRIQTQ KDQSFIELVK TVHQNVLFSQ QHEYFPLYEI QNHTELKQNL
360 370 380 390 400
IDHIMVIENY PLVEELQKNS IMQKVGFTVR DVKMFEPTNY DMTVMVLPRD
410 420 430 440 450
EISVRLDYNA AVYDIDFIRK IEGHMKEVAL CVANNPHVLV QDVPLLTKQE
460 470 480 490 500
KQHLLVELHD SITEYPDKTI HQLFTEQVEK TPEHVAVVFE DEKVTYRELH
510 520 530 540 550
ERSNQLARFL REKGVKKESI IGIMMERSVE MIVGILGILK AGGAFVPIDP
560 570 580 590 600
EYPKERIGYM LDSVRLVLTQ RHLKDKFAFT KETIVIEDPS ISHELTEEID
610 620 630 640 650
YINESEDLFY IIYTSGTTGK PKGVMLEHKN IVNLLHFTFE KTNINFSDKV
660 670 680 690 700
LQYTTCSFDV CYQEIFSTLL SGGQLYLIRK ETQRDVEQLF DLVKRENIEV
710 720 730 740 750
LSFPVAFLKF IFNEREFINR FPTCVKHIIT AGEQLVVNNE FKRYLHEHNV
760 770 780 790 800
HLHNHYGPSE THVVTTYTIN PEAEIPELPP IGKPISNTWI YILDQEQQLQ
810 820 830 840 850
PQGIVGELYI SGANVGRGYL NNQELTAEKF FADPFRPNER MYRTGDLARW
860 870 880 890 900
LPDGNIEFLG RADHQVKIRG HRIELGEIEA QLLNCKGVKE AVVIDKADDK
910 920 930 940 950
GGKYLCAYVV MEVEVNDSEL REYLGKALPD YMIPSFFVPL DQLPLTPNGK
960 970 980 990 1000
IDRKSLPNLE GIVNTNAKYV VPTNELEEKL AKIWEEVLGI SQIGIQDNFF
1010 1020 1030 1040 1050
SLGGHSLKAI TLISRMNKEC NVDIPLRLLF EAPTIQEISN YINGAKKESY
1060 1070 1080 1090 1100
VAIQPVPEQE YYPVSSVQKR MFILNEFDRS GTAYNLPGVM FLDGKLNYRQ
1110 1120 1130 1140 1150
LEAAVKKLVE RHEALRTSFH SINGEPVQRV HQNVELQIAY SESTEDQVER
1160 1170 1180 1190 1200
IIAEFMQPFA LEVAPLLRVG LVKLEAERHL FIMDMHHIIS DGVSMQIMIQ
1210 1220 1230 1240 1250
EIADLYKEKE LPTLGIQYKD FTVWHNRLLQ SDVIEKQEAY WLNVFTEEIP
1260 1270 1280 1290 1300
VLNLPTDYPR PTIQSFDGKR FTFSTGKQLM DDLYKVATET GTTLYMVLLA
1310 1320 1330 1340 1350
AYNVFLSKYS GQDDIVVGTP IAGRSHADVE NMLGMFVNTL AIRSRLNNED
1360 1370 1380 1390 1400
TFKDFLANVK QTALHAYENP DYPFDTLVEK LGIQRDLSRN PLFDTMFVLQ
1410 1420 1430 1440 1450
NTDRKSFEVE QITITPYVPN SRHSKFDLTL EVSEEQNEIL LCLEYCTKLF
1460 1470 1480 1490 1500
TDKTVERMAG HFLQILHAIV GNPTIIISEI EILSEEEKQH ILFEFNDTKT
1510 1520 1530 1540 1550
TYPHMQTIQG LFEEQVEKTP DHVAVGWKDQ ALTYRELNER ANQVARVLRQ
1560 1570 1580 1590 1600
KGVQPDNIVG LLVERSPEML VGIMGILKAG GAYLPLDPEY PADRISYMIQ
1610 1620 1630 1640 1650
DCGVRIMLTQ QHLLSLVHDE FDCVILDEDS LYKGDSSNLA PVNQAGDLAY
1660 1670 1680 1690 1700
IMYTSGSTGK PKGVMVEHRN VIRLVKNTNY VQVREDDRII QTGAIGFDAL
1710 1720 1730 1740 1750
TFEVFGSLLH GAELYPVTKD VLLDAEKLHK FLQANQITIM WLTSPLFNQL
1760 1770 1780 1790 1800
SQGTEEMFAG LRSLIVGGDA LSPKHINNVK RKCPNLTMWN GYGPTENTTF
1810 1820 1830 1840 1850
STCFLIDKEY DDNIPIGKAI SNSTVYIMDR YGQLQPVGVP GELCVGGDGV
1860 1870 1880 1890 1900
ARGYMNQPAL TEEKFVPNPF APGERMYRTG DLARWLPDGT IEYLGRIDQQ
1910 1920 1930 1940 1950
VKIRGYRIEP GEIETLLVKH KKVKESVIMV VEDNNGQKAL CAYYVPEEEV
1960 1970 1980 1990 2000
TVSELREYIA KELPVYMVPA YFVQIEQMPL TQNGKVNRSA LPKPDGEFGT
2010 2020 2030 2040 2050
ATEYVAPSSD IEMKLAEIWH NVLGVNKIGV LDNFFELGGH SLRAMTMISQ
2060 2070 2080 2090 2100
VHKEFDVELP LKVLFETPTI SALAQYIADG EKGMYLAIQP VTPQDYYPVS
2110 2120 2130 2140 2150
SAQKRMYILY EFEGAGITYN VPNVMFIEGK LDYQRFEYAI KSLINRHEAL
2160 2170 2180 2190 2200
RTSFYSLNGE PVQRVHQNVE LQIAYSEAKE DEIEQIVESF VQPFDLEIAP
2210 2220 2230 2240 2250
ALRVGLVKLA SDRHLFLMDM HHIISDGVSM QIITKEIADL YKGKELAELH
2260 2270 2280 2290 2300
IQYKDFAVWQ NEWFQSAALE KQKTYWLNTF AEDIPVLNLS TDYPRPTIQS
2310 2320 2330 2340 2350
FEGDIVTFSA GKQLAEELKR LATETGTTLY MLLLAAYNVL LHKYSGQEEI
2360 2370 2380 2390 2400
VVGTPIAGRS HADVENIVGM FVNTLALKNT PIAVRTFHEF LLEVKQNALE
2410 2420 2430 2440 2450
AFENQDYPFE NLIEKLQVRR DLSRNPLFDT MFSLSNIDEQ VEIGIEGLSF
2460 2470 2480 2490 2500
SPYEMQYWIA KFDISFDILE KQDDIQFYFN YCTNLFKKET IERLATHFMH
2510 2520 2530 2540 2550
ILQEIVINPE IKLCEINMLS EEEQQRVLYD FNGTDATYAT NKIFHELFEE
2560 2570 2580 2590 2600
QVEKTPDHIA VIDEREKLSY QELNAKANQL ARVLRQKGVQ PNSMVGIMVD
2610 2620 2630 2640 2650
RSLDMIVGML GVLKAGGAYV PIDIDYPQER ISYMMEDSGA ALLLTQQKLT
2660 2670 2680 2690 2700
QQIAFSGDIL YLDQEEWLHE EASNLEPIAR PQDIAYIIYT SGTTGKPKGV
2710 2720 2730 2740 2750
MIEHQSYVNV AMAWKDAYRL DTFPVRLLQM ASFAFDVSAG DFARALLTGG
2760 2770 2780 2790 2800
QLIVCPNEVK MDPASLYAII KKYDITIFEA TPALVIPLME YIYEQKLDIS
2810 2820 2830 2840 2850
QLQILIVGSD SCSMEDFKTL VSRFGSTIRI VNSYGVTEAC IDSSYYEQPL
2860 2870 2880 2890 2900
SSLHVTGTVP IGKPYANMKM YIMNQYLQIQ PVGVIGELCI GGAGVARGYL
2910 2920 2930 2940 2950
NRPDLTAEKF VPNPFVPGEK LYRTGDLARW MPDGNVEFLG RNDHQVKIRG
2960 2970 2980 2990 3000
IRIELGEIEA QLRKHDSIKE ATVIAREDHM KEKYLCAYMV TEGEVNVAEL
3010 3020 3030 3040 3050
RAYLATDLPA AMIPSYFVSL EAMPLTANGK IDKRSLPEPD GSISIGTEYV
3060 3070 3080 3090 3100
APRTMLEGKL EEIWKDVLGL QRVGIHDDFF TIGGHSLKAM AVISQVHKEC
3110 3120 3130 3140 3150
QTEVPLRVLF ETPTIQGLAK YIEETDTEQY MAIQPVSGQD YYPVSSAQKR
3160 3170 3180 3190 3200
MFIVNQFDGV GISYNMPSIM LIEGKLERTR LESAFKRLIE RHESLRTSFE
3210 3220 3230 3240 3250
IINGKPVQKI HEEADFNMSY QVASNEQVEK MIDEFIQPFD LSVAPLLRVE
3260 3270 3280 3290 3300
LLKLEEDRHV LIFDMHHIIS DGISSNILMK ELGELYQGNA LPELRIQYKD
3310 3320 3330 3340 3350
FAVWQNEWFQ SEAFKKQEEY WVNVFADERP ILDIPTDYPR PMQQSFDGAQ
3360 3370 3380 3390 3400
LTFGTGKQLM DGLYRVATET GTTLYMVLLA AYNVLLSKYS GQEDIIVGTP
3410 3420 3430 3440 3450
IVGRSHTDLE NIVGMFVNTL AMRNKPEGEK TFKAFVSEIK QNALAAFENQ
3460 3470 3480 3490 3500
DYPFEELIEK LEIQRDLSRN PLFDTLFSLQ NIGEESFELA ELTCKPFDLV
3510 3520 3530 3540 3550
SKLEHAKFDL SLVAVEKEEE IAFGLQYCTK LYKEKTVEQL AQHFIQIVKA
3560 3570 3580 3590 3600
IVENPDVKLS DIDMLSEEEK KQIMLEFNDT KIQYPQNQTI QELFEEQVKK
3610 3620 3630 3640 3650
TPEHIAIVWE GQALTYHELN IKANQLARVL REKGVTPNHP VAIMTERSLE
3660 3670 3680 3690 3700
MIVGIFSILK AGGAYVPIDP AYPQERIQYL LEDSGATLLL TQSHVLNKLP
3710 3720 3730 3740 3750
VDIEWLDLTD EQNYVEDGTN LPFMNQSTDL AYIIYTSGTT GKPKGVMIEH
3760 3770 3780 3790 3800
QSIINCLQWR KEEYEFGPGD TALQVFSFAF DGFVASLFAP ILAGATSVLP
3810 3820 3830 3840 3850
KEEEAKDPVA LKKLIASEEI THYYGVPSLF SAILDVSSSK DLQNLRCVTL
3860 3870 3880 3890 3900
GGEKLPAQIV KKIKEKNKEI EVNNEYGPTE NSVVTTIMRD IQVEQEITIG
3910 3920 3930 3940 3950
CPLSNVDVYI VNCNHQLQPV GVVGELCIGG QGLARGYLNK PELTADKFVV
3960 3970 3980 3990 4000
NPFVPGERMY KTGDLAKWRS DGMIEYVGRV DEQVKVRGYR IELGEIESAI
4010 4020 4030 4040 4050
LEYEKIKEAV VIVSEHTASE QMLCAYIVGE EDVLTLDLRS YLAKLLPSYM
4060 4070 4080 4090 4100
IPNYFIQLDS IPLTPNGKVD RKALPEPQTI GLMAREYVAP RNEIEAQLVL
4110 4120 4130 4140 4150
IWQEVLGIEL IGITDNFFEL GGHSLKATLL VAKIYEYMQI EMPLNVVFKH
4160 4170 4180 4190 4200
STIMKIAEYI THQESENNVH QPILVNVEAD REALSLNGEK QRKNIELPIL
4210 4220 4230 4240 4250
LNEETDRNVF LFAPIGAQGV FYKKLAEQIP TASLYGFDFI EDDDRIQQYI
4260 4270 4280 4290 4300
ESMIQTQSDG QYVLIGYSSG GNLAFEVAKE MERQGYSVSD LVLFDVYWKG
4310 4320 4330 4340 4350
KVFEQTKEEE EENIKIIMEE LRENPGMFNM TREDFELYFA NEFVKQSFTR
4360 4370 4380 4390 4400
KMRKYMSFYT QLVNYGEVEA TIHLIQAEFE EEKIDENEKA DEEEKTYLEE
4410 4420 4430 4440 4450
KWNEKAWNKA AKRFVKYNGY GAHSNMLGGD GLERNSSILK QILQGTFVVK
Length:4,450
Mass (Da):508,684
Last modified:January 23, 2007 - v2
Checksum:iF3197E77BF69316D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D29676 Genomic DNA. Translation: BAA06146.1.
D00938 Genomic DNA. Translation: BAA00778.1.
PIRiJX0340.
S20542. YGBSG2.

Similar proteinsi

Entry informationi

Entry nameiGRSB_BREBE
AccessioniPrimary (citable) accession number: P0C064
Secondary accession number(s): P14688, Q44928
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: January 23, 2007
Last modified: October 25, 2017
This is version 57 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families