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Protein

Small heat shock protein IbpB

Gene

ibpB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Associates with aggregated proteins, together with IbpA, to stabilize and protect them from irreversible denaturation and extensive proteolysis during heat shock and oxidative stress. Aggregated proteins bound to the IbpAB complex are more efficiently refolded and reactivated by the ATP-dependent chaperone systems ClpB and DnaK/DnaJ/GrpE. Its activity is ATP-independent.3 Publications

Temperature dependencei

Thermostable up to 50 degrees Celsius.

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

GO - Biological processi

  • protein stabilization Source: UniProtKB-HAMAP
  • response to heat Source: EcoCyc
  • stress response to copper ion Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciEcoCyc:EG11535-MONOMER.
ECOL316407:JW3663-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Small heat shock protein IbpB
Alternative name(s):
16 kDa heat shock protein B
Gene namesi
Name:ibpB
Synonyms:hslS, htpE
Ordered Locus Names:b3686, JW3663
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11535. ibpB.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001260281 – 142Small heat shock protein IbpBAdd BLAST142

Proteomic databases

PaxDbiP0C058.
PRIDEiP0C058.

Expressioni

Inductioni

By heat shock.1 Publication

Interactioni

Subunit structurei

Homodimer. Forms homomultimers of about 100-150 subunits at optimal growth temperatures. Conformation changes to oligomers at high temperatures or high ionic concentrations. The decrease in size of the multimers is accompanied by an increase in chaperone activity.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-552784,EBI-552784
ibpAP0C0543EBI-552784,EBI-550729

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4260809. 224 interactors.
DIPiDIP-48244N.
IntActiP0C058. 11 interactors.
MINTiMINT-1223453.
STRINGi511145.b3686.

Structurei

3D structure databases

ProteinModelPortaliP0C058.
SMRiP0C058.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N- and C-terminal flexible termini are involved in oligomerization and in the binding of non-native substrate proteins, and are essential for chaperone activity.1 Publication

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108SHS. Bacteria.
COG0071. LUCA.
HOGENOMiHOG000251750.
InParanoidiP0C058.
KOiK04081.
OMAiKESEYLH.
PhylomeDBiP0C058.

Family and domain databases

Gene3Di2.60.40.790. 1 hit.
HAMAPiMF_02001. HSP20_IbpB. 1 hit.
InterProiIPR002068. A-crystallin/Hsp20_dom.
IPR031107. HSP20.
IPR008978. HSP20-like_chaperone.
IPR022848. HSP20_IbpB.
[Graphical view]
PANTHERiPTHR11527. PTHR11527. 1 hit.
PfamiPF00011. HSP20. 1 hit.
[Graphical view]
SUPFAMiSSF49764. SSF49764. 1 hit.
PROSITEiPS01031. HSP20. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C058-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNFDLSPLM RQWIGFDKLA NALQNAGESQ SFPPYNIEKS DDNHYRITLA
60 70 80 90 100
LAGFRQEDLE IQLEGTRLSV KGTPEQPKEE KKWLHQGLMN QPFSLSFTLA
110 120 130 140
ENMEVSGATF VNGLLHIDLI RNEPEPIAAQ RIAISERPAL NS
Length:142
Mass (Da):16,093
Last modified:July 5, 2005 - v1
Checksum:i7666BB1BDB8A673A
GO

Sequence cautioni

The sequence AAA62038 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94104 Genomic DNA. Translation: AAA24425.1.
L10328 Genomic DNA. Translation: AAA62038.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76709.2.
AP009048 Genomic DNA. Translation: BAE77608.1.
RefSeqiNP_418141.2. NC_000913.3.
WP_001243431.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76709; AAC76709; b3686.
BAE77608; BAE77608; BAE77608.
GeneIDi948192.
KEGGiecj:JW3663.
eco:b3686.
PATRICi32122865. VBIEscCol129921_3808.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94104 Genomic DNA. Translation: AAA24425.1.
L10328 Genomic DNA. Translation: AAA62038.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76709.2.
AP009048 Genomic DNA. Translation: BAE77608.1.
RefSeqiNP_418141.2. NC_000913.3.
WP_001243431.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0C058.
SMRiP0C058.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260809. 224 interactors.
DIPiDIP-48244N.
IntActiP0C058. 11 interactors.
MINTiMINT-1223453.
STRINGi511145.b3686.

Proteomic databases

PaxDbiP0C058.
PRIDEiP0C058.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76709; AAC76709; b3686.
BAE77608; BAE77608; BAE77608.
GeneIDi948192.
KEGGiecj:JW3663.
eco:b3686.
PATRICi32122865. VBIEscCol129921_3808.

Organism-specific databases

EchoBASEiEB1497.
EcoGeneiEG11535. ibpB.

Phylogenomic databases

eggNOGiENOG4108SHS. Bacteria.
COG0071. LUCA.
HOGENOMiHOG000251750.
InParanoidiP0C058.
KOiK04081.
OMAiKESEYLH.
PhylomeDBiP0C058.

Enzyme and pathway databases

BioCyciEcoCyc:EG11535-MONOMER.
ECOL316407:JW3663-MONOMER.

Miscellaneous databases

PROiP0C058.

Family and domain databases

Gene3Di2.60.40.790. 1 hit.
HAMAPiMF_02001. HSP20_IbpB. 1 hit.
InterProiIPR002068. A-crystallin/Hsp20_dom.
IPR031107. HSP20.
IPR008978. HSP20-like_chaperone.
IPR022848. HSP20_IbpB.
[Graphical view]
PANTHERiPTHR11527. PTHR11527. 1 hit.
PfamiPF00011. HSP20. 1 hit.
[Graphical view]
SUPFAMiSSF49764. SSF49764. 1 hit.
PROSITEiPS01031. HSP20. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIBPB_ECOLI
AccessioniPrimary (citable) accession number: P0C058
Secondary accession number(s): P29210, P76733, Q2M7Z8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2005
Last modified: November 2, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.