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Protein

F protein

Gene
N/A
Organism
Hepatitis C virus genotype 1a (isolate H) (HCV)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • complement binding Source: AgBase
  • enzyme binding Source: AgBase
  • glycoprotein binding Source: AgBase
  • protein binding involved in protein folding Source: AgBase
  • Rab GTPase binding Source: AgBase
  • serpin family protein binding Source: AgBase
  • structural molecule activity Source: InterPro
  • zymogen binding Source: AgBase

GO - Biological processi

  • negative regulation of cytoskeleton organization Source: AgBase
  • protein folding Source: GOC
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
F protein
Alternative name(s):
Alternate reading frame protein/F-protein
Short name:
ARFP/F
Frameshifted protein
p16
p17
OrganismiHepatitis C virus genotype 1a (isolate H) (HCV)
Taxonomic identifieri11108 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageFlaviviridaeHepacivirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000000518 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

  • host cell nucleus Source: AgBase
  • host cell perinuclear region of cytoplasm Source: UniProtKB-SubCell
  • viral capsid Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed; by hostBy similarity
Chaini2 – 162161F proteinPRO_0000109553Add
BLAST

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PCNXL4Q63HM23EBI-9351969,EBI-1104540From a different organism.
PFDN2Q9UHV94EBI-9351969,EBI-359873From a different organism.

Protein-protein interaction databases

IntActiP0C045. 13 interactions.

Structurei

3D structure databases

ProteinModelPortaliP0C045.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Family and domain databases

InterProiIPR002522. HCV_core_N.
[Graphical view]
PfamiPF01543. HCV_capsid. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. AlignAdd to basket

Note: The exact location of the ribosomal frameshift is unknown. The F protein seems to be generated by a -2 ribosomal frameshift located in the vicinity of codon 11 of the core protein coding sequence. However, some F proteins may also be generated by +1 ribosomal frameshift. Since the core gene encodes alternative reading frame proteins (ARFPs), many functions depicted for the core protein might belong to the ARFPs.

Isoform F protein (identifier: P0C045-1) [UniParc]FASTAAdd to basket

Also known as: Frameshifted protein

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTNPKPQRK KPNVTPTVAH RTSSSRVAVR SLVEFTCCRA GALDWVCARR
60 70 80 90 100
GRLPSGRNLE VDVSLSPRHV GPRAGPGLSP GTLGPSMAMR VAGGRDGSCL
110 120 130 140 150
PVALGLAGAP QTPGVGRAIW VRSSIPLRAA SPTSWGTYRS SAPLLEALPG
160
PWRMASGFWK TA
Note: Produced by ribosomal frameshifting.
Length:162
Mass (Da):17,006
Last modified:January 23, 2007 - v2
Checksum:iAE9D5391D19211B6
GO
Isoform Genome polyprotein (identifier: P27958-1) [UniParc]FASTAAdd to basket

The sequence of this isoform can be found in the external entry P27958.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by conventional translation.
Length:3,011
Mass (Da):327,146
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M67463 Genomic RNA. No translation available.
AF009606 Genomic RNA. No translation available.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M67463 Genomic RNA. No translation available.
AF009606 Genomic RNA. No translation available.

3D structure databases

ProteinModelPortaliP0C045.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP0C045. 13 interactions.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

euHCVdbiAF009606.
M67463.

Family and domain databases

InterProiIPR002522. HCV_core_N.
[Graphical view]
PfamiPF01543. HCV_capsid. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genomic structure of the human prototype strain H of hepatitis C virus: comparison with American and Japanese isolates."
    Inchauspe G., Zebedee S., Lee D.H.H., Sugitani M., Nasoff M., Prince A.M.
    Proc. Natl. Acad. Sci. U.S.A. 88:10292-10296(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Transmission of hepatitis C by intrahepatic inoculation with transcribed RNA."
    Kolykhalov A.A., Agapov E.V., Blight K.J., Mihalik K., Feinstone S.M., Rice C.M.
    Science 277:570-574(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate H77.
  3. "Characterization of the expression of the hepatitis C virus F protein."
    Roussel J., Pillez A., Montpellier C., Duverlie G., Cahour A., Dubuisson J., Wychowski C.
    J. Gen. Virol. 84:1751-1759(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, SUBCELLULAR LOCATION.
  4. "The hepatitis C virus alternate reading frame (ARF) and its family of novel products: the alternate reading frame protein/F-protein, the double-frameshift protein, and others."
    Branch A.D., Stump D.D., Gutierrez J.A., Eng F., Walewski J.L.
    Semin. Liver Dis. 25:105-117(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiF_HCVH
AccessioniPrimary (citable) accession number: P0C045
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 40 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is very unstable.

Keywords - Technical termi

Complete proteome, Reference proteome

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.