P0C045 (F_HCVH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: F protein Alternative name(s): Alternate reading frame protein/F-protein Short name=ARFP/F Frameshifted protein p16 p17
Organism	Hepatitis C virus genotype 1a (isolate H) (HCV) [Reference proteome]
Taxonomic identifier	11108 [NCBI]
Taxonomic lineage	Viruses › ssRNA viruses › ssRNA positive-strand viruses, no DNA stage › Flaviviridae › Hepacivirus › Hepatitis C virus › Hepatitis C virus genotype 1 › Hepatitis C virus subtype 1a
Virus host	Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	162 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Host cytoplasm. Host cytoplasm › host perinuclear region Ref.3.
Miscellaneous	This protein is very unstable.

Ontologies

Keywords
Cellular component	Host cytoplasm
Coding sequence diversity	Ribosomal frameshifting
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	host cell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell viral capsid Inferred from electronic annotation. Source: InterPro
Molecular_function	structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]

Note: The exact location of the ribosomal frameshift is unknown. The F protein seems to be generated by a -2 ribosomal frameshift located in the vicinity of codon 11 of the core protein coding sequence. However, some F proteins may also be generated by +1 ribosomal frameshift. Since the core gene encodes alternative reading frame proteins (ARFPs), many functions depicted for the core protein might belong to the ARFPs.

Isoform F protein (identifier: P0C045-1)

Also known as: Frameshifted protein;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Produced by ribosomal frameshifting.

Isoform Genome polyprotein (identifier: P27958-1)

The sequence of this isoform can be found in the external entry P27958.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by conventional translation.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed; by host By similarity
	Chain	2 – 162	161	F protein		PRO_0000109553

Sequences

	Sequence		Length	Mass (Da)
	Isoform F protein (Frameshifted protein) [UniParc]. Last modified January 23, 2007. Version 2. Checksum: AE9D5391D19211B6	FASTA	162	17,006
	10 20 30 40 50 60 MSTNPKPQRK KPNVTPTVAH RTSSSRVAVR SLVEFTCCRA GALDWVCARR GRLPSGRNLE 70 80 90 100 110 120 VDVSLSPRHV GPRAGPGLSP GTLGPSMAMR VAGGRDGSCL PVALGLAGAP QTPGVGRAIW 130 140 150 160 VRSSIPLRAA SPTSWGTYRS SAPLLEALPG PWRMASGFWK TA
	Isoform Genome polyprotein [UniParc]. See P27958.		–	–

References

[1]	"Genomic structure of the human prototype strain H of hepatitis C virus: comparison with American and Japanese isolates." Inchauspe G., Zebedee S., Lee D.H.H., Sugitani M., Nasoff M., Prince A.M. Proc. Natl. Acad. Sci. U.S.A. 88:10292-10296(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Transmission of hepatitis C by intrahepatic inoculation with transcribed RNA." Kolykhalov A.A., Agapov E.V., Blight K.J., Mihalik K., Feinstone S.M., Rice C.M. Science 277:570-574(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate H77.
[3]	"Characterization of the expression of the hepatitis C virus F protein." Roussel J., Pillez A., Montpellier C., Duverlie G., Cahour A., Dubuisson J., Wychowski C. J. Gen. Virol. 84:1751-1759(2003) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION, SUBCELLULAR LOCATION.
[4]	"The hepatitis C virus alternate reading frame (ARF) and its family of novel products: the alternate reading frame protein/F-protein, the double-frameshift protein, and others." Branch A.D., Stump D.D., Gutierrez J.A., Eng F., Walewski J.L. Semin. Liver Dis. 25:105-117(2005) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M67463 Genomic RNA. No translation available. AF009606 Genomic RNA. No translation available.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
euHCVdb	AF009606. M67463.
Family and domain databases
InterPro	IPR002522. HCV_core_N. [Graphical view]
Pfam	PF01543. HCV_capsid. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F_HCVH

Accession

Primary (citable) accession number: P0C045

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 33 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program