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Protein

Pyrimidine/purine nucleoside phosphorylase

Gene

ppnP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorolysis of diverse nucleosides, yielding D-ribose 1-phosphate and the respective free bases. Can use uridine, adenosine, guanosine, cytidine, thymidine, inosine and xanthosine as substrates. Also catalyzes the reverse reactions. Is not able to produce D-ribose 1-phosphate from D-ribose and phosphate.1 Publication

Catalytic activityi

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.1 Publication
Adenosine + phosphate = adenine + alpha-D-ribose 1-phosphate.1 Publication
Cytidine + phosphate = cytosine + alpha-D-ribose 1-phosphate.1 Publication
Guanosine + phosphate = guanine + alpha-D-ribose 1-phosphate.1 Publication
Inosine + phosphate = hypoxanthine + alpha-D-ribose 1-phosphate.1 Publication
Thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate.1 Publication
Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate.1 Publication
Xanthosine + phosphate = xanthine + alpha-D-ribose 1-phosphate.1 Publication

GO - Molecular functioni

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:EG12159-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrimidine/purine nucleoside phosphorylase1 PublicationUniRule annotation (EC:2.4.2.1UniRule annotation1 Publication, EC:2.4.2.2UniRule annotation1 Publication)
Alternative name(s):
Adenosine phosphorylase1 Publication
Cytidine phosphorylase1 Publication
Guanosine phosphorylase1 Publication (EC:2.4.2.151 Publication)
Inosine phosphorylase1 Publication
Thymidine phosphorylase1 Publication (EC:2.4.2.41 Publication)
Uridine phosphorylase1 Publication (EC:2.4.2.31 Publication)
Xanthosine phosphorylase1 Publication
Gene namesi
Name:ppnP1 Publication
Synonyms:yaiEImported
Ordered Locus Names:b0391, JW0382
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12159. yaiE.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene show a consistent change in xanthine level.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002117631 – 94Pyrimidine/purine nucleoside phosphorylaseAdd BLAST94

Proteomic databases

EPDiP0C037.
PaxDbiP0C037.
PRIDEiP0C037.

Interactioni

Protein-protein interaction databases

BioGridi4259817. 9 interactors.
IntActiP0C037. 9 interactors.
STRINGi316385.ECDH10B_0348.

Structurei

3D structure databases

ProteinModelPortaliP0C037.
SMRiP0C037.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the nucleoside phosphorylase PpnP family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105NBK. Bacteria.
COG3123. LUCA.
HOGENOMiHOG000218057.
InParanoidiP0C037.
KOiK09913.
PhylomeDBiP0C037.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_01537. Nucleos_phosphorylase_PpnP. 1 hit.
InterProiView protein in InterPro
IPR009664. Ppnp.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
PfamiView protein in Pfam
PF06865. DUF1255. 1 hit.
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

P0C037-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLQSNEYFSG KVKSIGFSSS STGRASVGVM VEGEYTFSTA EPEEMTVISG
60 70 80 90
ALNVLLPDAT DWQVYEAGSV FNVPGHSEFH LQVAEPTSYL CRYL
Length:94
Mass (Da):10,234
Last modified:July 5, 2005 - v1
Checksum:iD7EF5C0AFD86D661
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti77 – 94SEFHL…LCRYL → TVSFICKLPNPPLICAAICN SSPSP in CAA54286 (PubMed:8807285).CuratedAdd BLAST18

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76979 Genomic DNA. Translation: CAA54286.1.
U73857 Genomic DNA. Translation: AAB18115.1.
U00096 Genomic DNA. Translation: AAC73494.1.
AP009048 Genomic DNA. Translation: BAE76172.1.
PIRiG64767.
RefSeqiNP_414925.1. NC_000913.3.
WP_000941942.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73494; AAC73494; b0391.
BAE76172; BAE76172; BAE76172.
GeneIDi945048.
KEGGiecj:JW0382.
eco:b0391.
PATRICifig|511145.12.peg.403.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPPNP_ECOLI
AccessioniPrimary (citable) accession number: P0C037
Secondary accession number(s): P36768, P77343, Q2MC34
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2005
Last modified: July 5, 2017
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries