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Protein

Diphosphoinositol polyphosphate phosphohydrolase 3-alpha

Gene

Nudt10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves a beta-phosphate from the diphosphate groups in PP-InsP5 (diphosphoinositol pentakisphosphate), suggesting that it may play a role in signal transduction. Also able to catalyze the hydrolysis of dinucleoside oligophosphates, with Ap6A and Ap5A being the preferred substrates. The major reaction products are ADP and p4a from Ap6A and ADP and ATP from Ap5A. Also able to hydrolyze 5-phosphoribose 1-diphosphate; however, the relevance of such activity in vivo remains unclear.

Miscellaneous

Nudt10 and Nudt11 code for identical proteins, which gives their indidual characterization difficult. Thus, most experiments do not discriminate between the 2 proteins.

Catalytic activityi

Diphospho-myo-inositol polyphosphate + H2O = myo-inositol polyphosphate + phosphate.1 Publication
P1,P6-bis(5'-adenosyl)hexaphosphate + H2O = adenosine 5'-pentaphosphate + AMP.1 Publication
P1,P5-bis(5'-adenosyl)pentaphosphate + H2O = adenosine 5'-tetraphosphate + AMP.1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 3 Mg2+ or Mn2+ ions per subunit. Mn2+ may be the true cofactor in vivo.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9SubstrateBy similarity1
Binding sitei40SubstrateBy similarity1
Metal bindingi49Magnesium 1; via carbonyl oxygenBy similarity1
Metal bindingi65Magnesium 2By similarity1
Metal bindingi65Magnesium 3By similarity1
Active sitei68Proton acceptorBy similarity1
Metal bindingi69Magnesium 1By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1855167 Synthesis of pyrophosphates in the cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
Diphosphoinositol polyphosphate phosphohydrolase 3-alpha (EC:3.6.1.521 Publication)
Short name:
DIPP-3-alpha
Short name:
DIPP3-alpha
Alternative name(s):
Diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase 3-alpha
Diadenosine hexaphosphate hydrolase (AMP-forming) (EC:3.6.1.601 Publication)
Nucleoside diphosphate-linked moiety X motif 10
Short name:
Nudix motif 10
Gene namesi
Name:Nudt10
Synonyms:Dipp3a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:2147931 Nudt10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000570631 – 164Diphosphoinositol polyphosphate phosphohydrolase 3-alphaAdd BLAST164

Proteomic databases

PaxDbiP0C027
PeptideAtlasiP0C027
PRIDEiP0C027

PTM databases

iPTMnetiP0C027
PhosphoSitePlusiP0C027

Expressioni

Tissue specificityi

Mainly expressed in testis, liver kidney and, at lower level, in heart, brain, spleen, lung and skeletal muscle.1 Publication

Gene expression databases

BgeeiENSMUSG00000073293
ExpressionAtlasiP0C027 baseline and differential
GenevisibleiP0C027 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000100072

Structurei

3D structure databases

ProteinModelPortaliP0C027
SMRiP0C027
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 144Nudix hydrolasePROSITE-ProRule annotationAdd BLAST128

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni17 – 19Substrate bindingBy similarity3
Regioni89 – 91Substrate bindingBy similarity3

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi50 – 71Nudix boxAdd BLAST22

Sequence similaritiesi

Belongs to the Nudix hydrolase family. DIPP subfamily.Curated

Phylogenomic databases

eggNOGiKOG2839 Eukaryota
COG0494 LUCA
HOGENOMiHOG000237336
HOVERGENiHBG053341
InParanoidiP0C027
KOiK07766
OMAiSKRHNMV
OrthoDBiEOG091G0TH6
PhylomeDBiP0C027

Family and domain databases

InterProiView protein in InterPro
IPR020476 Nudix_hydrolase
IPR015797 NUDIX_hydrolase-like_dom_sf
IPR020084 NUDIX_hydrolase_CS
IPR000086 NUDIX_hydrolase_dom
PfamiView protein in Pfam
PF00293 NUDIX, 1 hit
PRINTSiPR00502 NUDIXFAMILY
SUPFAMiSSF55811 SSF55811, 1 hit
PROSITEiView protein in PROSITE
PS51462 NUDIX, 1 hit
PS00893 NUDIX_BOX, 1 hit

Sequencei

Sequence statusi: Complete.

P0C027-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKCKPNQTRT YDPEGFKKRA ACLCFRSERE DEVLLVSSSR YPDRWIVPGG
60 70 80 90 100
GMEPEEEPDG AAVREVYEEA GVKGKLGRLL GVFEQNQDRK HRTYVFVLTV
110 120 130 140 150
TELLEDWEDS VSIGRKREWF KIEDAIKVLQ CHKPVHAEYL EKLKLGGSPT
160
NGNSAAPSPP ESEP
Length:164
Mass (Da):18,593
Last modified:July 5, 2005 - v1
Checksum:i7141513979E4FBCD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC055771 mRNA Translation: AAH55771.1
CCDSiCCDS29957.1
RefSeqiNP_001026834.1, NM_001031664.1
NP_067406.2, NM_021431.2
XP_006527609.1, XM_006527546.2
UniGeneiMm.41198
Mm.426101

Genome annotation databases

EnsembliENSMUST00000103006; ENSMUSP00000100071; ENSMUSG00000073293
ENSMUST00000103007; ENSMUSP00000100072; ENSMUSG00000073295
GeneIDi102954
58242
KEGGimmu:102954
mmu:58242
UCSCiuc009sku.1 mouse

Similar proteinsi

Entry informationi

Entry nameiNUD10_MOUSE
AccessioniPrimary (citable) accession number: P0C027
Secondary accession number(s): Q8BKF4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2005
Last modified: March 28, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families
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