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Protein

Diphosphoinositol polyphosphate phosphohydrolase 3-alpha

Gene

Nudt10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves a beta-phosphate from the diphosphate groups in PP-InsP5 (diphosphoinositol pentakisphosphate), suggesting that it may play a role in signal transduction. Also able to catalyze the hydrolysis of dinucleoside oligophosphates, with Ap6A and Ap5A being the preferred substrates. The major reaction products are ADP and p4a from Ap6A and ADP and ATP from Ap5A. Also able to hydrolyze 5-phosphoribose 1-diphosphate; however, the relevance of such activity in vivo remains unclear.

Catalytic activityi

Diphospho-myo-inositol polyphosphate + H2O = myo-inositol polyphosphate + phosphate.1 Publication
P1,P(6)-bis(5'-adenosyl)hexaphosphate + H2O = adenosine 5'-pentaphosphate + AMP.1 Publication
P1,P(5)-bis(5'-adenosyl)pentaphosphate + H2O = adenosine 5'-tetraphosphate + AMP.1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 3 Mg(2+) or Mn(2+) ions per subunit. Mn2+ may be the true cofactor in vivo.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91SubstrateBy similarity
Binding sitei40 – 401SubstrateBy similarity
Metal bindingi49 – 491Magnesium 1; via carbonyl oxygenBy similarity
Metal bindingi65 – 651Magnesium 2By similarity
Metal bindingi65 – 651Magnesium 3By similarity
Active sitei68 – 681Proton acceptorBy similarity
Metal bindingi69 – 691Magnesium 1By similarity

GO - Molecular functioni

  1. diphosphoinositol-polyphosphate diphosphatase activity Source: UniProtKB-EC
  2. inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 1-diphosphatase activity Source: UniProtKB-EC
  3. inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity Source: UniProtKB-EC
  4. inositol-1-diphosphate-2,3,4,5,6-pentakisphosphate diphosphatase activity Source: UniProtKB-EC
  5. inositol-3,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity Source: UniProtKB-EC
  6. inositol-3-diphosphate-1,2,4,5,6-pentakisphosphate diphosphatase activity Source: UniProtKB-EC
  7. inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activity Source: UniProtKB-EC
  8. inositol diphosphate tetrakisphosphate diphosphatase activity Source: UniProtKB-EC
  9. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_196482. Synthesis of pyrophosphates in the cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Diphosphoinositol polyphosphate phosphohydrolase 3-alpha (EC:3.6.1.52)
Short name:
DIPP-3-alpha
Short name:
DIPP3-alpha
Alternative name(s):
Diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase 3-alpha
Diadenosine hexaphosphate hydrolase (AMP-forming) (EC:3.6.1.60)
Nucleoside diphosphate-linked moiety X motif 10
Short name:
Nudix motif 10
Gene namesi
Name:Nudt10
Synonyms:Dipp3a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:2147931. Nudt10.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 164164Diphosphoinositol polyphosphate phosphohydrolase 3-alphaPRO_0000057063Add
BLAST

Proteomic databases

PaxDbiP0C027.
PRIDEiP0C027.

PTM databases

PhosphoSiteiP0C027.

Expressioni

Tissue specificityi

Mainly expressed in testis, liver kidney and, at lower level, in heart, brain, spleen, lung and skeletal muscle.1 Publication

Gene expression databases

BgeeiP0C027.
ExpressionAtlasiP0C027. baseline and differential.
GenevestigatoriP0C027.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000100072.

Structurei

3D structure databases

ProteinModelPortaliP0C027.
SMRiP0C027. Positions 17-144.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 144128Nudix hydrolasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni17 – 193Substrate bindingBy similarity
Regioni89 – 913Substrate bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi50 – 7122Nudix boxAdd
BLAST

Sequence similaritiesi

Belongs to the Nudix hydrolase family. DIPP subfamily.Curated
Contains 1 nudix hydrolase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG250169.
HOGENOMiHOG000237336.
HOVERGENiHBG053341.
InParanoidiP0C027.
KOiK07766.
OrthoDBiEOG7T7GVQ.
PhylomeDBiP0C027.

Family and domain databases

Gene3Di3.90.79.10. 1 hit.
InterProiIPR020476. Nudix_hydrolase.
IPR020084. NUDIX_hydrolase_CS.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
[Graphical view]
PfamiPF00293. NUDIX. 1 hit.
[Graphical view]
PRINTSiPR00502. NUDIXFAMILY.
SUPFAMiSSF55811. SSF55811. 1 hit.
PROSITEiPS51462. NUDIX. 1 hit.
PS00893. NUDIX_BOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C027-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKCKPNQTRT YDPEGFKKRA ACLCFRSERE DEVLLVSSSR YPDRWIVPGG
60 70 80 90 100
GMEPEEEPDG AAVREVYEEA GVKGKLGRLL GVFEQNQDRK HRTYVFVLTV
110 120 130 140 150
TELLEDWEDS VSIGRKREWF KIEDAIKVLQ CHKPVHAEYL EKLKLGGSPT
160
NGNSAAPSPP ESEP
Length:164
Mass (Da):18,593
Last modified:July 5, 2005 - v1
Checksum:i7141513979E4FBCD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC055771 mRNA. Translation: AAH55771.1.
CCDSiCCDS29957.1.
RefSeqiNP_001026834.1. NM_001031664.1.
NP_067406.2. NM_021431.2.
XP_006527609.1. XM_006527546.1.
UniGeneiMm.41198.
Mm.426101.

Genome annotation databases

EnsembliENSMUST00000103006; ENSMUSP00000100071; ENSMUSG00000073293.
ENSMUST00000103007; ENSMUSP00000100072; ENSMUSG00000073295.
GeneIDi102954.
58242.
KEGGimmu:102954.
mmu:58242.
UCSCiuc009sku.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC055771 mRNA. Translation: AAH55771.1.
CCDSiCCDS29957.1.
RefSeqiNP_001026834.1. NM_001031664.1.
NP_067406.2. NM_021431.2.
XP_006527609.1. XM_006527546.1.
UniGeneiMm.41198.
Mm.426101.

3D structure databases

ProteinModelPortaliP0C027.
SMRiP0C027. Positions 17-144.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000100072.

PTM databases

PhosphoSiteiP0C027.

Proteomic databases

PaxDbiP0C027.
PRIDEiP0C027.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103006; ENSMUSP00000100071; ENSMUSG00000073293.
ENSMUST00000103007; ENSMUSP00000100072; ENSMUSG00000073295.
GeneIDi102954.
58242.
KEGGimmu:102954.
mmu:58242.
UCSCiuc009sku.1. mouse.

Organism-specific databases

CTDi170685.
55190.
MGIiMGI:2147931. Nudt10.

Phylogenomic databases

eggNOGiNOG250169.
HOGENOMiHOG000237336.
HOVERGENiHBG053341.
InParanoidiP0C027.
KOiK07766.
OrthoDBiEOG7T7GVQ.
PhylomeDBiP0C027.

Enzyme and pathway databases

ReactomeiREACT_196482. Synthesis of pyrophosphates in the cytosol.

Miscellaneous databases

NextBioi314289.
PROiP0C027.
SOURCEiSearch...

Gene expression databases

BgeeiP0C027.
ExpressionAtlasiP0C027. baseline and differential.
GenevestigatoriP0C027.

Family and domain databases

Gene3Di3.90.79.10. 1 hit.
InterProiIPR020476. Nudix_hydrolase.
IPR020084. NUDIX_hydrolase_CS.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
[Graphical view]
PfamiPF00293. NUDIX. 1 hit.
[Graphical view]
PRINTSiPR00502. NUDIXFAMILY.
SUPFAMiSSF55811. SSF55811. 1 hit.
PROSITEiPS51462. NUDIX. 1 hit.
PS00893. NUDIX_BOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Paralogous murine Nudt10 and Nudt11 genes have differential expression patterns but encode identical proteins that are physiologically competent diphosphoinositol polyphosphate phosphohydrolases."
    Hua L.V., Hidaka K., Pesesse X., Barnes L.D., Shears S.B.
    Biochem. J. 373:81-89(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain and Embryo.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.

Entry informationi

Entry nameiNUD10_MOUSE
AccessioniPrimary (citable) accession number: P0C027
Secondary accession number(s): Q8BKF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2005
Last modified: January 7, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Nudt10 and Nudt11 code for identical proteins, which gives their indidual characterization difficult. Thus, most experiments do not discriminate between the 2 proteins.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.