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P0C027 (NUD10_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Diphosphoinositol polyphosphate phosphohydrolase 3-alpha

Short name=DIPP-3-alpha
Short name=DIPP3-alpha
EC=3.6.1.52
Alternative name(s):
Diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase 3-alpha
Diadenosine hexaphosphate hydrolase (AMP-forming)
EC=3.6.1.60
Nucleoside diphosphate-linked moiety X motif 10
Short name=Nudix motif 10
Gene names
Name:Nudt10
Synonyms:Dipp3a
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length164 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cleaves a beta-phosphate from the diphosphate groups in PP-InsP5 (diphosphoinositol pentakisphosphate), suggesting that it may play a role in signal transduction. Also able to catalyze the hydrolysis of dinucleoside oligophosphates, with Ap6A and Ap5A being the preferred substrates. The major reaction products are ADP and p4a from Ap6A and ADP and ATP from Ap5A. Also able to hydrolyze 5-phosphoribose 1-diphosphate; however, the relevance of such activity in vivo remains unclear.

Catalytic activity

Diphospho-myo-inositol polyphosphate + H2O = myo-inositol polyphosphate + phosphate. Ref.1

P1,P(6)-bis(5'-adenosyl)hexaphosphate + H2O = adenosine 5'-pentaphosphate + AMP. Ref.1

P1,P(5)-bis(5'-adenosyl)pentaphosphate + H2O = adenosine 5'-tetraphosphate + AMP. Ref.1

Cofactor

Binds 3 magnesium or manganese ions per subunit. Manganese may be the true cofactor in vivo By similarity.

Subcellular location

Cytoplasm By similarity.

Tissue specificity

Mainly expressed in testis, liver kidney and, at lower level, in heart, brain, spleen, lung and skeletal muscle. Ref.1

Miscellaneous

Nudt10 and Nudt11 code for identical proteins, which gives their indidual characterization difficult. Thus, most experiments do not discriminate between the 2 proteins.

Sequence similarities

Belongs to the Nudix hydrolase family. DIPP subfamily.

Contains 1 nudix hydrolase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 164164Diphosphoinositol polyphosphate phosphohydrolase 3-alpha
PRO_0000057063

Regions

Domain17 – 144128Nudix hydrolase
Region17 – 193Substrate binding By similarity
Region89 – 913Substrate binding By similarity
Motif50 – 7122Nudix box

Sites

Active site681Proton acceptor By similarity
Metal binding491Magnesium 1; via carbonyl oxygen By similarity
Metal binding651Magnesium 2 By similarity
Metal binding651Magnesium 3 By similarity
Metal binding691Magnesium 1 By similarity
Binding site91Substrate By similarity
Binding site401Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P0C027 [UniParc].

Last modified July 5, 2005. Version 1.
Checksum: 7141513979E4FBCD

FASTA16418,593
        10         20         30         40         50         60 
MKCKPNQTRT YDPEGFKKRA ACLCFRSERE DEVLLVSSSR YPDRWIVPGG GMEPEEEPDG 

        70         80         90        100        110        120 
AAVREVYEEA GVKGKLGRLL GVFEQNQDRK HRTYVFVLTV TELLEDWEDS VSIGRKREWF 

       130        140        150        160 
KIEDAIKVLQ CHKPVHAEYL EKLKLGGSPT NGNSAAPSPP ESEP 

« Hide

References

« Hide 'large scale' references
[1]"Paralogous murine Nudt10 and Nudt11 genes have differential expression patterns but encode identical proteins that are physiologically competent diphosphoinositol polyphosphate phosphohydrolases."
Hua L.V., Hidaka K., Pesesse X., Barnes L.D., Shears S.B.
Biochem. J. 373:81-89(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain and Embryo.
[3]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic brain.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC055771 mRNA. Translation: AAH55771.1.
CCDSCCDS29957.1.
RefSeqNP_001026834.1. NM_001031664.1.
NP_067406.2. NM_021431.2.
XP_006527609.1. XM_006527546.1.
UniGeneMm.41198.
Mm.426101.

3D structure databases

ProteinModelPortalP0C027.
SMRP0C027. Positions 17-144.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000100072.

PTM databases

PhosphoSiteP0C027.

Proteomic databases

PaxDbP0C027.
PRIDEP0C027.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000103006; ENSMUSP00000100071; ENSMUSG00000073293.
ENSMUST00000103007; ENSMUSP00000100072; ENSMUSG00000073295.
GeneID102954.
58242.
KEGGmmu:102954.
mmu:58242.
UCSCuc009sku.1. mouse.

Organism-specific databases

CTD170685.
55190.
MGIMGI:2147931. Nudt10.

Phylogenomic databases

eggNOGNOG250169.
HOGENOMHOG000237336.
HOVERGENHBG053341.
InParanoidP0C027.
KOK07766.
OrthoDBEOG7T7GVQ.
PhylomeDBP0C027.

Gene expression databases

ArrayExpressP0C027.
BgeeP0C027.
GenevestigatorP0C027.

Family and domain databases

Gene3D3.90.79.10. 1 hit.
InterProIPR020476. Nudix_hydrolase.
IPR020084. NUDIX_hydrolase_CS.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
[Graphical view]
PfamPF00293. NUDIX. 1 hit.
[Graphical view]
PRINTSPR00502. NUDIXFAMILY.
SUPFAMSSF55811. SSF55811. 1 hit.
PROSITEPS51462. NUDIX. 1 hit.
PS00893. NUDIX_BOX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio314289.
PROP0C027.
SOURCESearch...

Entry information

Entry nameNUD10_MOUSE
AccessionPrimary (citable) accession number: P0C027
Secondary accession number(s): Q8BKF4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2005
Last modified: July 9, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot