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P0C027

- NUD10_MOUSE

UniProt

P0C027 - NUD10_MOUSE

Protein

Diphosphoinositol polyphosphate phosphohydrolase 3-alpha

Gene

Nudt10

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (05 Jul 2005)
      Previous versions | rss
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    Functioni

    Cleaves a beta-phosphate from the diphosphate groups in PP-InsP5 (diphosphoinositol pentakisphosphate), suggesting that it may play a role in signal transduction. Also able to catalyze the hydrolysis of dinucleoside oligophosphates, with Ap6A and Ap5A being the preferred substrates. The major reaction products are ADP and p4a from Ap6A and ADP and ATP from Ap5A. Also able to hydrolyze 5-phosphoribose 1-diphosphate; however, the relevance of such activity in vivo remains unclear.

    Catalytic activityi

    Diphospho-myo-inositol polyphosphate + H2O = myo-inositol polyphosphate + phosphate.1 Publication
    P1,P(6)-bis(5'-adenosyl)hexaphosphate + H2O = adenosine 5'-pentaphosphate + AMP.1 Publication
    P1,P(5)-bis(5'-adenosyl)pentaphosphate + H2O = adenosine 5'-tetraphosphate + AMP.1 Publication

    Cofactori

    Binds 3 magnesium or manganese ions per subunit. Manganese may be the true cofactor in vivo By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei9 – 91SubstrateBy similarity
    Binding sitei40 – 401SubstrateBy similarity
    Metal bindingi49 – 491Magnesium 1; via carbonyl oxygenBy similarity
    Metal bindingi65 – 651Magnesium 2By similarity
    Metal bindingi65 – 651Magnesium 3By similarity
    Active sitei68 – 681Proton acceptorBy similarity
    Metal bindingi69 – 691Magnesium 1By similarity

    GO - Molecular functioni

    1. diphosphoinositol-polyphosphate diphosphatase activity Source: UniProtKB-EC
    2. inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 1-diphosphatase activity Source: UniProtKB-EC
    3. inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity Source: UniProtKB-EC
    4. inositol-1-diphosphate-2,3,4,5,6-pentakisphosphate diphosphatase activity Source: UniProtKB-EC
    5. inositol-3,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity Source: UniProtKB-EC
    6. inositol-3-diphosphate-1,2,4,5,6-pentakisphosphate diphosphatase activity Source: UniProtKB-EC
    7. inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activity Source: UniProtKB-EC
    8. inositol diphosphate tetrakisphosphate diphosphatase activity Source: UniProtKB-EC
    9. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_196482. Synthesis of pyrophosphates in the cytosol.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diphosphoinositol polyphosphate phosphohydrolase 3-alpha (EC:3.6.1.52)
    Short name:
    DIPP-3-alpha
    Short name:
    DIPP3-alpha
    Alternative name(s):
    Diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase 3-alpha
    Diadenosine hexaphosphate hydrolase (AMP-forming) (EC:3.6.1.60)
    Nucleoside diphosphate-linked moiety X motif 10
    Short name:
    Nudix motif 10
    Gene namesi
    Name:Nudt10
    Synonyms:Dipp3a
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:2147931. Nudt10.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 164164Diphosphoinositol polyphosphate phosphohydrolase 3-alphaPRO_0000057063Add
    BLAST

    Proteomic databases

    PaxDbiP0C027.
    PRIDEiP0C027.

    PTM databases

    PhosphoSiteiP0C027.

    Expressioni

    Tissue specificityi

    Mainly expressed in testis, liver kidney and, at lower level, in heart, brain, spleen, lung and skeletal muscle.1 Publication

    Gene expression databases

    ArrayExpressiP0C027.
    BgeeiP0C027.
    GenevestigatoriP0C027.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000100072.

    Structurei

    3D structure databases

    ProteinModelPortaliP0C027.
    SMRiP0C027. Positions 17-144.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini17 – 144128Nudix hydrolasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni17 – 193Substrate bindingBy similarity
    Regioni89 – 913Substrate bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi50 – 7122Nudix boxAdd
    BLAST

    Sequence similaritiesi

    Belongs to the Nudix hydrolase family. DIPP subfamily.Curated
    Contains 1 nudix hydrolase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG250169.
    HOGENOMiHOG000237336.
    HOVERGENiHBG053341.
    InParanoidiP0C027.
    KOiK07766.
    OrthoDBiEOG7T7GVQ.
    PhylomeDBiP0C027.

    Family and domain databases

    Gene3Di3.90.79.10. 1 hit.
    InterProiIPR020476. Nudix_hydrolase.
    IPR020084. NUDIX_hydrolase_CS.
    IPR000086. NUDIX_hydrolase_dom.
    IPR015797. NUDIX_hydrolase_dom-like.
    [Graphical view]
    PfamiPF00293. NUDIX. 1 hit.
    [Graphical view]
    PRINTSiPR00502. NUDIXFAMILY.
    SUPFAMiSSF55811. SSF55811. 1 hit.
    PROSITEiPS51462. NUDIX. 1 hit.
    PS00893. NUDIX_BOX. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0C027-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKCKPNQTRT YDPEGFKKRA ACLCFRSERE DEVLLVSSSR YPDRWIVPGG    50
    GMEPEEEPDG AAVREVYEEA GVKGKLGRLL GVFEQNQDRK HRTYVFVLTV 100
    TELLEDWEDS VSIGRKREWF KIEDAIKVLQ CHKPVHAEYL EKLKLGGSPT 150
    NGNSAAPSPP ESEP 164
    Length:164
    Mass (Da):18,593
    Last modified:July 5, 2005 - v1
    Checksum:i7141513979E4FBCD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC055771 mRNA. Translation: AAH55771.1.
    CCDSiCCDS29957.1.
    RefSeqiNP_001026834.1. NM_001031664.1.
    NP_067406.2. NM_021431.2.
    XP_006527609.1. XM_006527546.1.
    UniGeneiMm.41198.
    Mm.426101.

    Genome annotation databases

    EnsembliENSMUST00000103006; ENSMUSP00000100071; ENSMUSG00000073293.
    ENSMUST00000103007; ENSMUSP00000100072; ENSMUSG00000073295.
    GeneIDi102954.
    58242.
    KEGGimmu:102954.
    mmu:58242.
    UCSCiuc009sku.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC055771 mRNA. Translation: AAH55771.1 .
    CCDSi CCDS29957.1.
    RefSeqi NP_001026834.1. NM_001031664.1.
    NP_067406.2. NM_021431.2.
    XP_006527609.1. XM_006527546.1.
    UniGenei Mm.41198.
    Mm.426101.

    3D structure databases

    ProteinModelPortali P0C027.
    SMRi P0C027. Positions 17-144.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000100072.

    PTM databases

    PhosphoSitei P0C027.

    Proteomic databases

    PaxDbi P0C027.
    PRIDEi P0C027.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000103006 ; ENSMUSP00000100071 ; ENSMUSG00000073293 .
    ENSMUST00000103007 ; ENSMUSP00000100072 ; ENSMUSG00000073295 .
    GeneIDi 102954.
    58242.
    KEGGi mmu:102954.
    mmu:58242.
    UCSCi uc009sku.1. mouse.

    Organism-specific databases

    CTDi 170685.
    55190.
    MGIi MGI:2147931. Nudt10.

    Phylogenomic databases

    eggNOGi NOG250169.
    HOGENOMi HOG000237336.
    HOVERGENi HBG053341.
    InParanoidi P0C027.
    KOi K07766.
    OrthoDBi EOG7T7GVQ.
    PhylomeDBi P0C027.

    Enzyme and pathway databases

    Reactomei REACT_196482. Synthesis of pyrophosphates in the cytosol.

    Miscellaneous databases

    NextBioi 314289.
    PROi P0C027.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P0C027.
    Bgeei P0C027.
    Genevestigatori P0C027.

    Family and domain databases

    Gene3Di 3.90.79.10. 1 hit.
    InterProi IPR020476. Nudix_hydrolase.
    IPR020084. NUDIX_hydrolase_CS.
    IPR000086. NUDIX_hydrolase_dom.
    IPR015797. NUDIX_hydrolase_dom-like.
    [Graphical view ]
    Pfami PF00293. NUDIX. 1 hit.
    [Graphical view ]
    PRINTSi PR00502. NUDIXFAMILY.
    SUPFAMi SSF55811. SSF55811. 1 hit.
    PROSITEi PS51462. NUDIX. 1 hit.
    PS00893. NUDIX_BOX. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Paralogous murine Nudt10 and Nudt11 genes have differential expression patterns but encode identical proteins that are physiologically competent diphosphoinositol polyphosphate phosphohydrolases."
      Hua L.V., Hidaka K., Pesesse X., Barnes L.D., Shears S.B.
      Biochem. J. 373:81-89(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain and Embryo.
    3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic brain.

    Entry informationi

    Entry nameiNUD10_MOUSE
    AccessioniPrimary (citable) accession number: P0C027
    Secondary accession number(s): Q8BKF4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2005
    Last sequence update: July 5, 2005
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Nudt10 and Nudt11 code for identical proteins, which gives their indidual characterization difficult. Thus, most experiments do not discriminate between the 2 proteins.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3