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Protein

Dihydropteroate synthase type-1

Gene

sulI

Organism
Corynebacterium glutamicum (Brevibacterium saccharolyticum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DHPS catalyzes the formation of the immediate precursor of folic acid. Implicated in resistance to sulfonamide. The type II enzyme is stable whereas type I DHPS loses its activity rapidly (By similarity).By similarity

Catalytic activityi

(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit. Magnesium is required for activity, even if it interacts primarily with the substrate.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91MagnesiumBy similarity
Binding sitei17 – 171SubstrateBy similarity
Binding sitei82 – 821SubstrateBy similarity
Binding sitei101 – 1011SubstrateBy similarity
Binding sitei173 – 1731SubstrateBy similarity
Binding sitei212 – 2121SubstrateBy similarity
Binding sitei246 – 2461SubstrateBy similarity
Binding sitei248 – 2481SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Antibiotic resistance, Folate biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00077; UER00156.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydropteroate synthase type-1 (EC:2.5.1.15)
Alternative name(s):
Dihydropteroate pyrophosphorylase type I
Dihydropteroate synthase type I
Short name:
DHPS
Gene namesi
Name:sulI
Encoded oniPlasmid pCG41 Publication
OrganismiCorynebacterium glutamicum (Brevibacterium saccharolyticum)
Taxonomic identifieri1718 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 279279Dihydropteroate synthase type-1PRO_0000168200Add
BLAST

Interactioni

Subunit structurei

Homodimer or homotrimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP0C004.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 258258Pterin-bindingPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni49 – 502Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the DHPS family.Curated
Contains 1 pterin-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK18974.

Family and domain databases

Gene3Di3.20.20.20. 1 hit.
InterProiIPR006390. DHP_synth.
IPR011005. Dihydropteroate_synth-like.
IPR000489. Pterin-binding.
[Graphical view]
PfamiPF00809. Pterin_bind. 1 hit.
[Graphical view]
SUPFAMiSSF51717. SSF51717. 1 hit.
TIGRFAMsiTIGR01496. DHPS. 1 hit.
PROSITEiPS00792. DHPS_1. 1 hit.
PS00793. DHPS_2. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C004-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTVFGILNL TEDSFFDESR RLDPAGAVTA AIEMLRVGSD VVDVGPAASH
60 70 80 90 100
PDARPVSPAD EIRRIAPLLD ALSDQMHRVS IDSFQPETQR YALKRGVGYL
110 120 130 140 150
NDIQGFPDPA LYPDIAEADC RLVVMHSAQR DGIATRTGHL RPEDALDEIV
160 170 180 190 200
RFFEARVSAL RRSGVAADRL ILDPGMGFFL SPAPETSLHV LSNLQKLKSA
210 220 230 240 250
LGLPLLVSVS RKSFLGATVG LPVKDLGPAS LAAELHAIGN GADYVRTHAP
260 270
GDLRSAITFS ETLAKFRSRD ARDRGLDHA
Length:279
Mass (Da):30,126
Last modified:June 7, 2005 - v1
Checksum:i13EE36C6E3FBDF63
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y14748 Genomic DNA. Translation: CAA75045.1.
RefSeqiNP_478074.1. NC_003227.1.
NP_862308.1. NC_004945.1.
WP_000259031.1. NC_004945.1.

Genome annotation databases

GeneIDi1132010.
1450507.
4999104.
KEGGipg:1132010.
pg:1450507.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y14748 Genomic DNA. Translation: CAA75045.1.
RefSeqiNP_478074.1. NC_003227.1.
NP_862308.1. NC_004945.1.
WP_000259031.1. NC_004945.1.

3D structure databases

ProteinModelPortaliP0C004.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1132010.
1450507.
4999104.
KEGGipg:1132010.
pg:1450507.

Phylogenomic databases

KOiK18974.

Enzyme and pathway databases

UniPathwayiUPA00077; UER00156.

Family and domain databases

Gene3Di3.20.20.20. 1 hit.
InterProiIPR006390. DHP_synth.
IPR011005. Dihydropteroate_synth-like.
IPR000489. Pterin-binding.
[Graphical view]
PfamiPF00809. Pterin_bind. 1 hit.
[Graphical view]
SUPFAMiSSF51717. SSF51717. 1 hit.
TIGRFAMsiTIGR01496. DHPS. 1 hit.
PROSITEiPS00792. DHPS_1. 1 hit.
PS00793. DHPS_2. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "An integron of class 1 is present on the plasmid pCG4 from Gram-positive bacterium Corynebacterium glutamicum."
    Nesvera J., Hochmannova J., Patek M.
    FEMS Microbiol. Lett. 169:391-395(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 31830 / T250.
    Plasmid: pCG4

Entry informationi

Entry nameiDHP1_CORGT
AccessioniPrimary (citable) accession number: P0C004
Secondary accession number(s): P11744, Q93K51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: May 27, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The sulI gene is located on various large self-transmissible resistance plasmids and on transposons related to Tn21.

Keywords - Technical termi

Plasmid, Transposable element

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.