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P0C002

- DHP1_ECOLX

UniProt

P0C002 - DHP1_ECOLX

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Protein
Dihydropteroate synthase type-1
Gene
sulI, sul1
Organism
Escherichia coli
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

DHPS catalyzes the formation of the immediate precursor of folic acid. Implicated in resistance to sulfonamide. The type II enzyme is stable whereas type I DHPS loses its activity rapidly.

Catalytic activityi

(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.

Cofactori

Binds 1 magnesium ion per subunit. Magnesium is required for activity, even if it interacts primarily with the substrate By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91Magnesium By similarity
Binding sitei17 – 171Substrate By similarity
Binding sitei82 – 821Substrate By similarity
Binding sitei101 – 1011Substrate By similarity
Binding sitei173 – 1731Substrate By similarity
Binding sitei212 – 2121Substrate By similarity
Binding sitei246 – 2461Substrate By similarity
Binding sitei248 – 2481Substrate By similarity

GO - Molecular functioni

  1. dihydropteroate synthase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. folic acid biosynthetic process Source: UniProtKB-KW
  2. response to antibiotic Source: UniProtKB-KW
  3. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Antibiotic resistance, Folate biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00077; UER00156.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydropteroate synthase type-1 (EC:2.5.1.15)
Alternative name(s):
Dihydropteroate pyrophosphorylase type I
Dihydropteroate synthase type I
Short name:
DHPS
Gene namesi
Name:sulI
Synonyms:sul1
Encoded oniPlasmid IncFII R100 (NR1)1 Publication
Plasmid IncW R3882 Publications
Plasmid pLMO201 Publication
Plasmid pLMO271 Publication
Plasmid IncN R462 Publications
Plasmid pDGO1001 Publication
Plasmid R6-51 Publication
Plasmid pDGO1011 Publication
Plasmid IncFII NR791 Publication
Plasmid IncW pSa3 Publications
Plasmid IncP-beta R7511 Publication
Plasmid pCMXR11 Publication
Plasmid pMSP0711 Publication
Plasmid p1658/971 Publication
Plasmid pHSH11 Publication
Plasmid pHSH21 Publication
Plasmid pAK331 Publication
Plasmid p5411 Publication
Plasmid pQR-11 Publication
Plasmid pKO561 Publication
Plasmid pKO971 Publication
Plasmid pAPEC-O2-R1 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 279279Dihydropteroate synthase type-1
PRO_0000168201Add
BLAST

Proteomic databases

PRIDEiP0C002.

Interactioni

Subunit structurei

Homodimer or homotrimer.

Structurei

3D structure databases

ProteinModelPortaliP0C002.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 258258Pterin-binding
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni49 – 502Substrate binding By similarity

Sequence similaritiesi

Belongs to the DHPS family.

Family and domain databases

Gene3Di3.20.20.20. 1 hit.
InterProiIPR006390. DHP_synth.
IPR011005. Dihydropteroate_synth-like.
IPR000489. Pterin-binding.
[Graphical view]
PfamiPF00809. Pterin_bind. 1 hit.
[Graphical view]
SUPFAMiSSF51717. SSF51717. 1 hit.
TIGRFAMsiTIGR01496. DHPS. 1 hit.
PROSITEiPS00792. DHPS_1. 1 hit.
PS00793. DHPS_2. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0C002-1 [UniParc]FASTAAdd to Basket

« Hide

MVTVFGILNL TEDSFFDESR RLDPAGAVTA AIEMLRVGSD VVDVGPAASH    50
PDARPVSPAD EIRRIAPLLD ALSDQMHRVS IDSFQPETQR YALKRGVGYL 100
NDIQGFPDPA LYPDIAEADC RLVVMHSAQR DGIATRTGHL RPEDALDEIV 150
RFFEARVSAL RRSGVAADRL ILDPGMGFFL SPAPETSLHV LSNLQKLKSA 200
LGLPLLVSVS RKSFLGATVG LPVKDLGPAS LAAELHAIGN GADYVRTHAP 250
GDLRSAITFS ETLAKFRSRD ARDRGLDHA 279
Length:279
Mass (Da):30,126
Last modified:June 7, 2005 - v1
Checksum:i13EE36C6E3FBDF63
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti259 – 2591F → I in CAA00729. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X12868 Genomic DNA. Translation: CAA31358.1.
M95287 Genomic DNA. Translation: AAB59087.1.
U42226 Genomic DNA. Translation: AAC53726.1.
X15024 Genomic DNA. Translation: CAA33123.1.
A07921 Unassigned DNA. Translation: CAA00729.1.
X12870 Genomic DNA. Translation: CAA31364.1.
AF047479 Genomic DNA. Translation: AAC14739.1.
L06418 Genomic DNA. Translation: AAA92747.1.
L06418 Genomic DNA. Translation: AAA92751.3.
U04277 Genomic DNA. Translation: AAB60179.1.
AF071413 Genomic DNA. Translation: AAC33914.1.
AF205943 Genomic DNA. Translation: AAG45723.1.
U12441 Genomic DNA. Translation: AAK95985.1.
AB061794 Genomic DNA. Translation: BAB72156.1.
AF174129 Genomic DNA. Translation: AAK60189.2.
AF550679 Genomic DNA. Translation: AAO49595.1.
AY259085 Genomic DNA. Translation: AAP20908.1.
AY259085 Genomic DNA. Translation: AAP20913.1.
AY259086 Genomic DNA. Translation: AAP20924.1.
AY259086 Genomic DNA. Translation: AAP20929.1.
AY224185 Genomic DNA. Translation: AAP51287.1.
AY260546 Genomic DNA. Translation: AAP22979.1.
AY339625 Genomic DNA. Translation: AAQ16671.1.
L06822 Genomic DNA. Translation: AAW29415.1.
L06822 Genomic DNA. Translation: AAW29418.1.
AY655485 Genomic DNA. Translation: AAW31097.1.
AY878717 Genomic DNA. Translation: AAX18266.1.
AY878717 Genomic DNA. Translation: AAX18269.1.
AY878718 Genomic DNA. Translation: AAX18276.1.
AY878718 Genomic DNA. Translation: AAX18279.1.
AY214164 Genomic DNA. Translation: AAP51280.1.
X58425 Genomic DNA. Translation: CAA41328.1.
PIRiA60174. SYECOG.
T45123.
RefSeqiNP_863000.1. NC_004998.1.
YP_001096354.1. NC_009132.1.
YP_001096411.1. NC_009133.1.
YP_002891164.1. NC_012690.1.
YP_002894492.1. NC_012692.1.
YP_003937681.1. NC_014615.1.
YP_004558213.1. NC_015599.1.
YP_006902980.1. NC_019037.1.
YP_006903340.1. NC_019045.2.
YP_006939957.1. NC_018994.1.
YP_006952407.1. NC_019062.1.
YP_006952437.1. NC_019063.1.
YP_006952978.1. NC_019066.1.
YP_006953197.1. NC_019069.1.
YP_006953612.1. NC_019081.1.
YP_006953620.1. NC_019082.1.
YP_008574825.1. NC_022374.1.
YP_009023104.1. NC_023909.1.
YP_190215.1. NC_006671.1.
YP_724471.1. NC_007682.3.

Genome annotation databases

GeneIDi10752055.
13876924.
13900729.
13903449.
13905341.
13905373.
13905882.
13906105.
13906547.
13906557.
1446559.
17035641.
18983605.
3244835.
4246764.
4924679.
4924736.
7872397.
7872757.
9846098.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X12868 Genomic DNA. Translation: CAA31358.1 .
M95287 Genomic DNA. Translation: AAB59087.1 .
U42226 Genomic DNA. Translation: AAC53726.1 .
X15024 Genomic DNA. Translation: CAA33123.1 .
A07921 Unassigned DNA. Translation: CAA00729.1 .
X12870 Genomic DNA. Translation: CAA31364.1 .
AF047479 Genomic DNA. Translation: AAC14739.1 .
L06418 Genomic DNA. Translation: AAA92747.1 .
L06418 Genomic DNA. Translation: AAA92751.3 .
U04277 Genomic DNA. Translation: AAB60179.1 .
AF071413 Genomic DNA. Translation: AAC33914.1 .
AF205943 Genomic DNA. Translation: AAG45723.1 .
U12441 Genomic DNA. Translation: AAK95985.1 .
AB061794 Genomic DNA. Translation: BAB72156.1 .
AF174129 Genomic DNA. Translation: AAK60189.2 .
AF550679 Genomic DNA. Translation: AAO49595.1 .
AY259085 Genomic DNA. Translation: AAP20908.1 .
AY259085 Genomic DNA. Translation: AAP20913.1 .
AY259086 Genomic DNA. Translation: AAP20924.1 .
AY259086 Genomic DNA. Translation: AAP20929.1 .
AY224185 Genomic DNA. Translation: AAP51287.1 .
AY260546 Genomic DNA. Translation: AAP22979.1 .
AY339625 Genomic DNA. Translation: AAQ16671.1 .
L06822 Genomic DNA. Translation: AAW29415.1 .
L06822 Genomic DNA. Translation: AAW29418.1 .
AY655485 Genomic DNA. Translation: AAW31097.1 .
AY878717 Genomic DNA. Translation: AAX18266.1 .
AY878717 Genomic DNA. Translation: AAX18269.1 .
AY878718 Genomic DNA. Translation: AAX18276.1 .
AY878718 Genomic DNA. Translation: AAX18279.1 .
AY214164 Genomic DNA. Translation: AAP51280.1 .
X58425 Genomic DNA. Translation: CAA41328.1 .
PIRi A60174. SYECOG.
T45123.
RefSeqi NP_863000.1. NC_004998.1.
YP_001096354.1. NC_009132.1.
YP_001096411.1. NC_009133.1.
YP_002891164.1. NC_012690.1.
YP_002894492.1. NC_012692.1.
YP_003937681.1. NC_014615.1.
YP_004558213.1. NC_015599.1.
YP_006902980.1. NC_019037.1.
YP_006903340.1. NC_019045.2.
YP_006939957.1. NC_018994.1.
YP_006952407.1. NC_019062.1.
YP_006952437.1. NC_019063.1.
YP_006952978.1. NC_019066.1.
YP_006953197.1. NC_019069.1.
YP_006953612.1. NC_019081.1.
YP_006953620.1. NC_019082.1.
YP_008574825.1. NC_022374.1.
YP_009023104.1. NC_023909.1.
YP_190215.1. NC_006671.1.
YP_724471.1. NC_007682.3.

3D structure databases

ProteinModelPortali P0C002.
ModBasei Search...

Proteomic databases

PRIDEi P0C002.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 10752055.
13876924.
13900729.
13903449.
13905341.
13905373.
13905882.
13906105.
13906547.
13906557.
1446559.
17035641.
18983605.
3244835.
4246764.
4924679.
4924736.
7872397.
7872757.
9846098.

Enzyme and pathway databases

UniPathwayi UPA00077 ; UER00156 .

Family and domain databases

Gene3Di 3.20.20.20. 1 hit.
InterProi IPR006390. DHP_synth.
IPR011005. Dihydropteroate_synth-like.
IPR000489. Pterin-binding.
[Graphical view ]
Pfami PF00809. Pterin_bind. 1 hit.
[Graphical view ]
SUPFAMi SSF51717. SSF51717. 1 hit.
TIGRFAMsi TIGR01496. DHPS. 1 hit.
PROSITEi PS00792. DHPS_1. 1 hit.
PS00793. DHPS_2. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Site-specific recombination promotes linkage between trimethoprim- and sulfonamide resistance genes. Sequence characterization of dhfrV and sulI and a recombination active locus of Tn21."
    Sundstroem L., Radstroem P., Swedberg G., Skoeld O.
    Mol. Gen. Genet. 213:191-201(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Transposon: Tn21.
    Plasmid: IncW R388 pLMO20
  2. "A novel family of potentially mobile DNA elements encoding site-specific gene-integration functions: integrons."
    Stokes H.W., Hall R.M.
    Mol. Microbiol. 3:1669-1683(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: IncFII R100 (NR1) IncN R46 pDGO101
  3. "Nucleotide sequence of the sulfonamide resistance gene from plasmid R46."
    Guerineau F., Mullineaux P.M.
    Nucleic Acids Res. 17:4370-4370(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: IncN R46
  4. Sundstroem L.
    Submitted (NOV-1989) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Transposon: Tn21.
    Plasmid: R6-5
  5. "The chloramphenicol acetyltransferase gene of Tn2424: a new breed of cat."
    Parent R., Roy P.H.
    J. Bacteriol. 174:2891-2897(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Transposon: Tn2424.
    Plasmid: IncFII NR79
  6. "The partial 3'-conserved segment duplications in the integrons In6 from pSa and In7 from pDGO100 have a common origin."
    Stokes H.W., Tomaras C., Parsons Y., Hall R.M.
    Plasmid 30:39-50(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 120-279.
    Plasmid: IncW pSa pDGO100
  7. "DNA sequence of direct repeats of the sulI gene of plasmid pSa."
    Valentine C.R., Heinrich M.J., Chissoe S.L., Roe B.A.
    Plasmid 32:222-227(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: IncW pSa
  8. "Transposon Tn21, flagship of the floating genome."
    Liebert C.A., Hall R.M., Summers A.O.
    Microbiol. Mol. Biol. Rev. 63:507-522(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Transposon: Tn21.
  9. "Characterization of In53, a class 1 plasmid- and composite transposon-located integron of Escherichia coli which carries an unusual array of gene cassettes."
    Naas T., Mikami Y., Imai T., Poirel L., Nordmann P.
    J. Bacteriol. 183:235-249(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: MG-1.
    Transposon: Tn2000.
    Plasmid: IncP-beta R751
  10. Partridge S.R., Hall R.M.
    Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: IncW R388
  11. "Characterization of a novel plasmid-mediated cephalosporinase (CMY-9) and its genetic environment in an Escherichia coli clinical isolate."
    Doi Y., Shibata N., Shibayama K., Kamachi K., Kurokawa H., Yokoyama K., Yagi T., Arakawa Y.
    Antimicrob. Agents Chemother. 46:2427-2434(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HKYM68.
    Plasmid: pCMXR1
  12. "Novel complex sul1-type integron in Escherichia coli carrying bla(CTX-M-9)."
    Sabate M., Navarro F., Miro E., Campoy S., Mirelis B., Barbe J., Prats G.
    Antimicrob. Agents Chemother. 46:2656-2661(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 785-D.
    Plasmid: pMSP071
  13. "p1658/97 complete sequence."
    Zienkiewicz M., Kern-Zdanowicz I., Golebiewski M., Ceglowski P.
    Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: p1658/97
  14. "Plasmid-mediated quinolone resistance in clinical isolates of Escherichia coli from Shanghai, China."
    Wang M., Tran J.H., Jacoby G.A., Zhang Y., Wang F., Hooper D.C.
    Antimicrob. Agents Chemother. 47:2242-2248(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: pHSH1 pHSH2
  15. "Decreased susceptibility to cefepime in a clinical strain of Escherichia coli related to plasmid- and integron-encoded OXA-30 beta-lactamase."
    Dubois V., Arpin C., Quentin C., Texier-Maugein J., Poirel L., Nordmann P.
    Antimicrob. Agents Chemother. 47:2380-2381(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  16. "Characterization of In111, a class 1 integron that carries the extended-spectrum beta, -lactamase gene blaIBC-1."
    Vourli S., Tzouvelekis L.S., Tzelepi E., Lebessi E., Legakis N.J., Miriagou V.
    FEMS Microbiol. Lett. 225:149-153(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: pAK33
  17. "CMY-13, a novel inducible cephalosporinase encoded by an Escherichia coli plasmid."
    Miriagou V., Tzouvelekis L.S., Villa L., Lebessi E., Vatopoulos A.C., Carattoli A., Tzelepi E.
    Antimicrob. Agents Chemother. 48:3172-3174(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: p541
  18. Partridge S.R., Hall R.M., Stokes H.W.
    Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: IncW pSa
  19. "Emergence of plasmid-mediated quinolone resistance in Escherichia coli in Europe."
    Mammeri H., Van De Loo M., Poirel L., Martinez-Martinez L., Nordmann P.
    Antimicrob. Agents Chemother. 49:71-76(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Loup.
    Plasmid: pQR-1
  20. "Detection of qnr in clinical isolates of Escherichia coli from Korea."
    Jeong J.-Y., Yoon H.J., Kim E.S., Lee Y., Choi S.-H., Kim N.J., Woo J.H., Kim Y.S.
    Antimicrob. Agents Chemother. 49:2522-2524(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: pKO56 pKO97
  21. Johnson T.J., Nolan L.K.
    Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: A2363.
    Plasmid: pAPEC-O2-R
  22. Sundstroem L., Swedberg G., Skoeld O.
    Submitted (MAR-1991) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-120.
    Transposon: Tn5086.
    Plasmid: pLMO27

Entry informationi

Entry nameiDHP1_ECOLX
AccessioniPrimary (citable) accession number: P0C002
Secondary accession number(s): P11744, Q79LJ7, Q93K51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: July 9, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The sulI gene is located on various large self-transmissible resistance plasmids and on transposons related to Tn21.
The plasmid pDGO100 contains two copies of the sulI gene.

Keywords - Technical termi

Plasmid, Transposable element

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi