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P0C002

- DHP1_ECOLX

UniProt

P0C002 - DHP1_ECOLX

Protein

Dihydropteroate synthase type-1

Gene

sulI

Organism
Escherichia coli
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (07 Jun 2005)
      Previous versions | rss
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    Functioni

    DHPS catalyzes the formation of the immediate precursor of folic acid. Implicated in resistance to sulfonamide. The type II enzyme is stable whereas type I DHPS loses its activity rapidly.

    Catalytic activityi

    (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.

    Cofactori

    Binds 1 magnesium ion per subunit. Magnesium is required for activity, even if it interacts primarily with the substrate By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi9 – 91MagnesiumBy similarity
    Binding sitei17 – 171SubstrateBy similarity
    Binding sitei82 – 821SubstrateBy similarity
    Binding sitei101 – 1011SubstrateBy similarity
    Binding sitei173 – 1731SubstrateBy similarity
    Binding sitei212 – 2121SubstrateBy similarity
    Binding sitei246 – 2461SubstrateBy similarity
    Binding sitei248 – 2481SubstrateBy similarity

    GO - Molecular functioni

    1. dihydropteroate synthase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. folic acid biosynthetic process Source: UniProtKB-KW
    2. response to antibiotic Source: UniProtKB-KW
    3. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Antibiotic resistance, Folate biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00077; UER00156.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dihydropteroate synthase type-1 (EC:2.5.1.15)
    Alternative name(s):
    Dihydropteroate pyrophosphorylase type I
    Dihydropteroate synthase type I
    Short name:
    DHPS
    Gene namesi
    Name:sulI
    Synonyms:sul1
    Encoded oniPlasmid IncFII R100 (NR1)1 Publication
    Plasmid IncW R3882 Publications
    Plasmid pLMO201 Publication
    Plasmid pLMO271 Publication
    Plasmid IncN R462 Publications
    Plasmid pDGO1001 Publication
    Plasmid R6-51 Publication
    Plasmid pDGO1011 Publication
    Plasmid IncFII NR791 Publication
    Plasmid IncW pSa3 Publications
    Plasmid IncP-beta R7511 Publication
    Plasmid pCMXR11 Publication
    Plasmid pMSP0711 Publication
    Plasmid p1658/971 Publication
    Plasmid pHSH11 Publication
    Plasmid pHSH21 Publication
    Plasmid pAK331 Publication
    Plasmid p5411 Publication
    Plasmid pQR-11 Publication
    Plasmid pKO561 Publication
    Plasmid pKO971 Publication
    Plasmid pAPEC-O2-R1 Publication
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 279279Dihydropteroate synthase type-1PRO_0000168201Add
    BLAST

    Proteomic databases

    PRIDEiP0C002.

    Interactioni

    Subunit structurei

    Homodimer or homotrimer.

    Structurei

    3D structure databases

    ProteinModelPortaliP0C002.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 258258Pterin-bindingPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni49 – 502Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the DHPS family.Curated
    Contains 1 pterin-binding domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.20.20.20. 1 hit.
    InterProiIPR006390. DHP_synth.
    IPR011005. Dihydropteroate_synth-like.
    IPR000489. Pterin-binding.
    [Graphical view]
    PfamiPF00809. Pterin_bind. 1 hit.
    [Graphical view]
    SUPFAMiSSF51717. SSF51717. 1 hit.
    TIGRFAMsiTIGR01496. DHPS. 1 hit.
    PROSITEiPS00792. DHPS_1. 1 hit.
    PS00793. DHPS_2. 1 hit.
    PS50972. PTERIN_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0C002-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVTVFGILNL TEDSFFDESR RLDPAGAVTA AIEMLRVGSD VVDVGPAASH    50
    PDARPVSPAD EIRRIAPLLD ALSDQMHRVS IDSFQPETQR YALKRGVGYL 100
    NDIQGFPDPA LYPDIAEADC RLVVMHSAQR DGIATRTGHL RPEDALDEIV 150
    RFFEARVSAL RRSGVAADRL ILDPGMGFFL SPAPETSLHV LSNLQKLKSA 200
    LGLPLLVSVS RKSFLGATVG LPVKDLGPAS LAAELHAIGN GADYVRTHAP 250
    GDLRSAITFS ETLAKFRSRD ARDRGLDHA 279
    Length:279
    Mass (Da):30,126
    Last modified:June 7, 2005 - v1
    Checksum:i13EE36C6E3FBDF63
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti259 – 2591F → I in CAA00729. (PubMed:2662140)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12868 Genomic DNA. Translation: CAA31358.1.
    M95287 Genomic DNA. Translation: AAB59087.1.
    U42226 Genomic DNA. Translation: AAC53726.1.
    X15024 Genomic DNA. Translation: CAA33123.1.
    A07921 Unassigned DNA. Translation: CAA00729.1.
    X12870 Genomic DNA. Translation: CAA31364.1.
    AF047479 Genomic DNA. Translation: AAC14739.1.
    L06418 Genomic DNA. Translation: AAA92747.1.
    L06418 Genomic DNA. Translation: AAA92751.3.
    U04277 Genomic DNA. Translation: AAB60179.1.
    AF071413 Genomic DNA. Translation: AAC33914.1.
    AF205943 Genomic DNA. Translation: AAG45723.1.
    U12441 Genomic DNA. Translation: AAK95985.1.
    AB061794 Genomic DNA. Translation: BAB72156.1.
    AF174129 Genomic DNA. Translation: AAK60189.2.
    AF550679 Genomic DNA. Translation: AAO49595.1.
    AY259085 Genomic DNA. Translation: AAP20908.1.
    AY259085 Genomic DNA. Translation: AAP20913.1.
    AY259086 Genomic DNA. Translation: AAP20924.1.
    AY259086 Genomic DNA. Translation: AAP20929.1.
    AY224185 Genomic DNA. Translation: AAP51287.1.
    AY260546 Genomic DNA. Translation: AAP22979.1.
    AY339625 Genomic DNA. Translation: AAQ16671.1.
    L06822 Genomic DNA. Translation: AAW29415.1.
    L06822 Genomic DNA. Translation: AAW29418.1.
    AY655485 Genomic DNA. Translation: AAW31097.1.
    AY878717 Genomic DNA. Translation: AAX18266.1.
    AY878717 Genomic DNA. Translation: AAX18269.1.
    AY878718 Genomic DNA. Translation: AAX18276.1.
    AY878718 Genomic DNA. Translation: AAX18279.1.
    AY214164 Genomic DNA. Translation: AAP51280.1.
    X58425 Genomic DNA. Translation: CAA41328.1.
    PIRiA60174. SYECOG.
    T45123.
    RefSeqiNP_863000.1. NC_004998.1.
    YP_001096354.1. NC_009132.1.
    YP_001096411.1. NC_009133.1.
    YP_002891164.1. NC_012690.1.
    YP_002894492.1. NC_012692.1.
    YP_003937681.1. NC_014615.1.
    YP_004558213.1. NC_015599.1.
    YP_006902980.1. NC_019037.1.
    YP_006903340.1. NC_019045.2.
    YP_006939957.1. NC_018994.1.
    YP_006952407.1. NC_019062.1.
    YP_006952437.1. NC_019063.1.
    YP_006952978.1. NC_019066.1.
    YP_006953197.1. NC_019069.1.
    YP_006953612.1. NC_019081.1.
    YP_006953620.1. NC_019082.1.
    YP_008574825.1. NC_022374.1.
    YP_009023104.1. NC_023909.1.
    YP_190215.1. NC_006671.1.
    YP_724471.1. NC_007682.3.

    Genome annotation databases

    GeneIDi10752055.
    13876924.
    13900729.
    13903449.
    13905341.
    13905373.
    13905882.
    13906105.
    13906547.
    13906557.
    1446559.
    17035641.
    18983605.
    3244835.
    4246764.
    4924679.
    4924736.
    7872397.
    7872757.
    9846098.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12868 Genomic DNA. Translation: CAA31358.1 .
    M95287 Genomic DNA. Translation: AAB59087.1 .
    U42226 Genomic DNA. Translation: AAC53726.1 .
    X15024 Genomic DNA. Translation: CAA33123.1 .
    A07921 Unassigned DNA. Translation: CAA00729.1 .
    X12870 Genomic DNA. Translation: CAA31364.1 .
    AF047479 Genomic DNA. Translation: AAC14739.1 .
    L06418 Genomic DNA. Translation: AAA92747.1 .
    L06418 Genomic DNA. Translation: AAA92751.3 .
    U04277 Genomic DNA. Translation: AAB60179.1 .
    AF071413 Genomic DNA. Translation: AAC33914.1 .
    AF205943 Genomic DNA. Translation: AAG45723.1 .
    U12441 Genomic DNA. Translation: AAK95985.1 .
    AB061794 Genomic DNA. Translation: BAB72156.1 .
    AF174129 Genomic DNA. Translation: AAK60189.2 .
    AF550679 Genomic DNA. Translation: AAO49595.1 .
    AY259085 Genomic DNA. Translation: AAP20908.1 .
    AY259085 Genomic DNA. Translation: AAP20913.1 .
    AY259086 Genomic DNA. Translation: AAP20924.1 .
    AY259086 Genomic DNA. Translation: AAP20929.1 .
    AY224185 Genomic DNA. Translation: AAP51287.1 .
    AY260546 Genomic DNA. Translation: AAP22979.1 .
    AY339625 Genomic DNA. Translation: AAQ16671.1 .
    L06822 Genomic DNA. Translation: AAW29415.1 .
    L06822 Genomic DNA. Translation: AAW29418.1 .
    AY655485 Genomic DNA. Translation: AAW31097.1 .
    AY878717 Genomic DNA. Translation: AAX18266.1 .
    AY878717 Genomic DNA. Translation: AAX18269.1 .
    AY878718 Genomic DNA. Translation: AAX18276.1 .
    AY878718 Genomic DNA. Translation: AAX18279.1 .
    AY214164 Genomic DNA. Translation: AAP51280.1 .
    X58425 Genomic DNA. Translation: CAA41328.1 .
    PIRi A60174. SYECOG.
    T45123.
    RefSeqi NP_863000.1. NC_004998.1.
    YP_001096354.1. NC_009132.1.
    YP_001096411.1. NC_009133.1.
    YP_002891164.1. NC_012690.1.
    YP_002894492.1. NC_012692.1.
    YP_003937681.1. NC_014615.1.
    YP_004558213.1. NC_015599.1.
    YP_006902980.1. NC_019037.1.
    YP_006903340.1. NC_019045.2.
    YP_006939957.1. NC_018994.1.
    YP_006952407.1. NC_019062.1.
    YP_006952437.1. NC_019063.1.
    YP_006952978.1. NC_019066.1.
    YP_006953197.1. NC_019069.1.
    YP_006953612.1. NC_019081.1.
    YP_006953620.1. NC_019082.1.
    YP_008574825.1. NC_022374.1.
    YP_009023104.1. NC_023909.1.
    YP_190215.1. NC_006671.1.
    YP_724471.1. NC_007682.3.

    3D structure databases

    ProteinModelPortali P0C002.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P0C002.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 10752055.
    13876924.
    13900729.
    13903449.
    13905341.
    13905373.
    13905882.
    13906105.
    13906547.
    13906557.
    1446559.
    17035641.
    18983605.
    3244835.
    4246764.
    4924679.
    4924736.
    7872397.
    7872757.
    9846098.

    Enzyme and pathway databases

    UniPathwayi UPA00077 ; UER00156 .

    Family and domain databases

    Gene3Di 3.20.20.20. 1 hit.
    InterProi IPR006390. DHP_synth.
    IPR011005. Dihydropteroate_synth-like.
    IPR000489. Pterin-binding.
    [Graphical view ]
    Pfami PF00809. Pterin_bind. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51717. SSF51717. 1 hit.
    TIGRFAMsi TIGR01496. DHPS. 1 hit.
    PROSITEi PS00792. DHPS_1. 1 hit.
    PS00793. DHPS_2. 1 hit.
    PS50972. PTERIN_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Site-specific recombination promotes linkage between trimethoprim- and sulfonamide resistance genes. Sequence characterization of dhfrV and sulI and a recombination active locus of Tn21."
      Sundstroem L., Radstroem P., Swedberg G., Skoeld O.
      Mol. Gen. Genet. 213:191-201(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Transposon: Tn21.
      Plasmid: IncW R388 pLMO20
    2. "A novel family of potentially mobile DNA elements encoding site-specific gene-integration functions: integrons."
      Stokes H.W., Hall R.M.
      Mol. Microbiol. 3:1669-1683(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Plasmid: IncFII R100 (NR1) IncN R46 pDGO101
    3. "Nucleotide sequence of the sulfonamide resistance gene from plasmid R46."
      Guerineau F., Mullineaux P.M.
      Nucleic Acids Res. 17:4370-4370(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Plasmid: IncN R46
    4. Sundstroem L.
      Submitted (NOV-1989) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Transposon: Tn21.
      Plasmid: R6-5
    5. "The chloramphenicol acetyltransferase gene of Tn2424: a new breed of cat."
      Parent R., Roy P.H.
      J. Bacteriol. 174:2891-2897(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Transposon: Tn2424.
      Plasmid: IncFII NR79
    6. "The partial 3'-conserved segment duplications in the integrons In6 from pSa and In7 from pDGO100 have a common origin."
      Stokes H.W., Tomaras C., Parsons Y., Hall R.M.
      Plasmid 30:39-50(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 120-279.
      Plasmid: IncW pSa pDGO100
    7. "DNA sequence of direct repeats of the sulI gene of plasmid pSa."
      Valentine C.R., Heinrich M.J., Chissoe S.L., Roe B.A.
      Plasmid 32:222-227(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Plasmid: IncW pSa
    8. "Transposon Tn21, flagship of the floating genome."
      Liebert C.A., Hall R.M., Summers A.O.
      Microbiol. Mol. Biol. Rev. 63:507-522(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Transposon: Tn21.
    9. "Characterization of In53, a class 1 plasmid- and composite transposon-located integron of Escherichia coli which carries an unusual array of gene cassettes."
      Naas T., Mikami Y., Imai T., Poirel L., Nordmann P.
      J. Bacteriol. 183:235-249(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: MG-1.
      Transposon: Tn2000.
      Plasmid: IncP-beta R751
    10. Partridge S.R., Hall R.M.
      Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Plasmid: IncW R388
    11. "Characterization of a novel plasmid-mediated cephalosporinase (CMY-9) and its genetic environment in an Escherichia coli clinical isolate."
      Doi Y., Shibata N., Shibayama K., Kamachi K., Kurokawa H., Yokoyama K., Yagi T., Arakawa Y.
      Antimicrob. Agents Chemother. 46:2427-2434(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: HKYM68.
      Plasmid: pCMXR1
    12. "Novel complex sul1-type integron in Escherichia coli carrying bla(CTX-M-9)."
      Sabate M., Navarro F., Miro E., Campoy S., Mirelis B., Barbe J., Prats G.
      Antimicrob. Agents Chemother. 46:2656-2661(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 785-D.
      Plasmid: pMSP071
    13. "p1658/97 complete sequence."
      Zienkiewicz M., Kern-Zdanowicz I., Golebiewski M., Ceglowski P.
      Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Plasmid: p1658/97
    14. "Plasmid-mediated quinolone resistance in clinical isolates of Escherichia coli from Shanghai, China."
      Wang M., Tran J.H., Jacoby G.A., Zhang Y., Wang F., Hooper D.C.
      Antimicrob. Agents Chemother. 47:2242-2248(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Plasmid: pHSH1 pHSH2
    15. "Decreased susceptibility to cefepime in a clinical strain of Escherichia coli related to plasmid- and integron-encoded OXA-30 beta-lactamase."
      Dubois V., Arpin C., Quentin C., Texier-Maugein J., Poirel L., Nordmann P.
      Antimicrob. Agents Chemother. 47:2380-2381(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    16. "Characterization of In111, a class 1 integron that carries the extended-spectrum beta, -lactamase gene blaIBC-1."
      Vourli S., Tzouvelekis L.S., Tzelepi E., Lebessi E., Legakis N.J., Miriagou V.
      FEMS Microbiol. Lett. 225:149-153(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Plasmid: pAK33
    17. "CMY-13, a novel inducible cephalosporinase encoded by an Escherichia coli plasmid."
      Miriagou V., Tzouvelekis L.S., Villa L., Lebessi E., Vatopoulos A.C., Carattoli A., Tzelepi E.
      Antimicrob. Agents Chemother. 48:3172-3174(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Plasmid: p541
    18. Partridge S.R., Hall R.M., Stokes H.W.
      Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Plasmid: IncW pSa
    19. "Emergence of plasmid-mediated quinolone resistance in Escherichia coli in Europe."
      Mammeri H., Van De Loo M., Poirel L., Martinez-Martinez L., Nordmann P.
      Antimicrob. Agents Chemother. 49:71-76(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Loup.
      Plasmid: pQR-1
    20. "Detection of qnr in clinical isolates of Escherichia coli from Korea."
      Jeong J.-Y., Yoon H.J., Kim E.S., Lee Y., Choi S.-H., Kim N.J., Woo J.H., Kim Y.S.
      Antimicrob. Agents Chemother. 49:2522-2524(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Plasmid: pKO56 pKO97
    21. Johnson T.J., Nolan L.K.
      Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: A2363.
      Plasmid: pAPEC-O2-R
    22. Sundstroem L., Swedberg G., Skoeld O.
      Submitted (MAR-1991) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-120.
      Transposon: Tn5086.
      Plasmid: pLMO27

    Entry informationi

    Entry nameiDHP1_ECOLX
    AccessioniPrimary (citable) accession number: P0C002
    Secondary accession number(s): P11744, Q79LJ7, Q93K51
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2005
    Last sequence update: June 7, 2005
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The sulI gene is located on various large self-transmissible resistance plasmids and on transposons related to Tn21.
    The plasmid pDGO100 contains two copies of the sulI gene.

    Keywords - Technical termi

    Plasmid, Transposable element

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3