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P0C002 (DHP1_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydropteroate synthase type-1

EC=2.5.1.15
Alternative name(s):
Dihydropteroate pyrophosphorylase type I
Dihydropteroate synthase type I
Short name=DHPS
Gene names
Name:sulI
Synonyms:sul1
Encoded onPlasmid IncFII R100 (NR1) Ref.2
Plasmid IncW R388 Ref.1 Ref.10
Plasmid pLMO20 Ref.1
Plasmid pLMO27 Ref.22
Plasmid IncN R46 Ref.2 Ref.3
Plasmid pDGO100 Ref.6
Plasmid R6-5 Ref.4
Plasmid pDGO101 Ref.2
Plasmid IncFII NR79 Ref.5
Plasmid IncW pSa Ref.6 Ref.7 Ref.18
Plasmid IncP-beta R751 Ref.9
Plasmid pCMXR1 Ref.11
Plasmid pMSP071 Ref.12
Plasmid p1658/97 Ref.13
Plasmid pHSH1 Ref.14
Plasmid pHSH2 Ref.14
Plasmid pAK33 Ref.16
Plasmid p541 Ref.17
Plasmid pQR-1 Ref.19
Plasmid pKO56 Ref.20
Plasmid pKO97 Ref.20
Plasmid pAPEC-O2-R Ref.21
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length279 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DHPS catalyzes the formation of the immediate precursor of folic acid. Implicated in resistance to sulfonamide. The type II enzyme is stable whereas type I DHPS loses its activity rapidly.

Catalytic activity

(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.

Cofactor

Binds 1 magnesium ion per subunit. Magnesium is required for activity, even if it interacts primarily with the substrate By similarity.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.

Subunit structure

Homodimer or homotrimer.

Miscellaneous

The sulI gene is located on various large self-transmissible resistance plasmids and on transposons related to Tn21.

The plasmid pDGO100 contains two copies of the sulI gene.

Sequence similarities

Belongs to the DHPS family.

Contains 1 pterin-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 279279Dihydropteroate synthase type-1
PRO_0000168201

Regions

Domain1 – 258258Pterin-binding
Region49 – 502Substrate binding By similarity

Sites

Metal binding91Magnesium By similarity
Binding site171Substrate By similarity
Binding site821Substrate By similarity
Binding site1011Substrate By similarity
Binding site1731Substrate By similarity
Binding site2121Substrate By similarity
Binding site2461Substrate By similarity
Binding site2481Substrate By similarity

Experimental info

Sequence conflict2591F → I in CAA00729. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P0C002 [UniParc].

Last modified June 7, 2005. Version 1.
Checksum: 13EE36C6E3FBDF63

FASTA27930,126
        10         20         30         40         50         60 
MVTVFGILNL TEDSFFDESR RLDPAGAVTA AIEMLRVGSD VVDVGPAASH PDARPVSPAD 

        70         80         90        100        110        120 
EIRRIAPLLD ALSDQMHRVS IDSFQPETQR YALKRGVGYL NDIQGFPDPA LYPDIAEADC 

       130        140        150        160        170        180 
RLVVMHSAQR DGIATRTGHL RPEDALDEIV RFFEARVSAL RRSGVAADRL ILDPGMGFFL 

       190        200        210        220        230        240 
SPAPETSLHV LSNLQKLKSA LGLPLLVSVS RKSFLGATVG LPVKDLGPAS LAAELHAIGN 

       250        260        270 
GADYVRTHAP GDLRSAITFS ETLAKFRSRD ARDRGLDHA 

« Hide

References

[1]"Site-specific recombination promotes linkage between trimethoprim- and sulfonamide resistance genes. Sequence characterization of dhfrV and sulI and a recombination active locus of Tn21."
Sundstroem L., Radstroem P., Swedberg G., Skoeld O.
Mol. Gen. Genet. 213:191-201(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Transposon: Tn21.
[2]"A novel family of potentially mobile DNA elements encoding site-specific gene-integration functions: integrons."
Stokes H.W., Hall R.M.
Mol. Microbiol. 3:1669-1683(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Nucleotide sequence of the sulfonamide resistance gene from plasmid R46."
Guerineau F., Mullineaux P.M.
Nucleic Acids Res. 17:4370-4370(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]Sundstroem L.
Submitted (NOV-1989) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Transposon: Tn21.
[5]"The chloramphenicol acetyltransferase gene of Tn2424: a new breed of cat."
Parent R., Roy P.H.
J. Bacteriol. 174:2891-2897(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Transposon: Tn2424.
[6]"The partial 3'-conserved segment duplications in the integrons In6 from pSa and In7 from pDGO100 have a common origin."
Stokes H.W., Tomaras C., Parsons Y., Hall R.M.
Plasmid 30:39-50(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 120-279.
[7]"DNA sequence of direct repeats of the sulI gene of plasmid pSa."
Valentine C.R., Heinrich M.J., Chissoe S.L., Roe B.A.
Plasmid 32:222-227(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[8]"Transposon Tn21, flagship of the floating genome."
Liebert C.A., Hall R.M., Summers A.O.
Microbiol. Mol. Biol. Rev. 63:507-522(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Transposon: Tn21.
[9]"Characterization of In53, a class 1 plasmid- and composite transposon-located integron of Escherichia coli which carries an unusual array of gene cassettes."
Naas T., Mikami Y., Imai T., Poirel L., Nordmann P.
J. Bacteriol. 183:235-249(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: MG-1.
Transposon: Tn2000.
[10]Partridge S.R., Hall R.M.
Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[11]"Characterization of a novel plasmid-mediated cephalosporinase (CMY-9) and its genetic environment in an Escherichia coli clinical isolate."
Doi Y., Shibata N., Shibayama K., Kamachi K., Kurokawa H., Yokoyama K., Yagi T., Arakawa Y.
Antimicrob. Agents Chemother. 46:2427-2434(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: HKYM68.
[12]"Novel complex sul1-type integron in Escherichia coli carrying bla(CTX-M-9)."
Sabate M., Navarro F., Miro E., Campoy S., Mirelis B., Barbe J., Prats G.
Antimicrob. Agents Chemother. 46:2656-2661(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 785-D.
[13]"p1658/97 complete sequence."
Zienkiewicz M., Kern-Zdanowicz I., Golebiewski M., Ceglowski P.
Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[14]"Plasmid-mediated quinolone resistance in clinical isolates of Escherichia coli from Shanghai, China."
Wang M., Tran J.H., Jacoby G.A., Zhang Y., Wang F., Hooper D.C.
Antimicrob. Agents Chemother. 47:2242-2248(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[15]"Decreased susceptibility to cefepime in a clinical strain of Escherichia coli related to plasmid- and integron-encoded OXA-30 beta-lactamase."
Dubois V., Arpin C., Quentin C., Texier-Maugein J., Poirel L., Nordmann P.
Antimicrob. Agents Chemother. 47:2380-2381(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[16]"Characterization of In111, a class 1 integron that carries the extended-spectrum beta, -lactamase gene blaIBC-1."
Vourli S., Tzouvelekis L.S., Tzelepi E., Lebessi E., Legakis N.J., Miriagou V.
FEMS Microbiol. Lett. 225:149-153(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[17]"CMY-13, a novel inducible cephalosporinase encoded by an Escherichia coli plasmid."
Miriagou V., Tzouvelekis L.S., Villa L., Lebessi E., Vatopoulos A.C., Carattoli A., Tzelepi E.
Antimicrob. Agents Chemother. 48:3172-3174(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[18]Partridge S.R., Hall R.M., Stokes H.W.
Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[19]"Emergence of plasmid-mediated quinolone resistance in Escherichia coli in Europe."
Mammeri H., Van De Loo M., Poirel L., Martinez-Martinez L., Nordmann P.
Antimicrob. Agents Chemother. 49:71-76(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Loup.
[20]"Detection of qnr in clinical isolates of Escherichia coli from Korea."
Jeong J.-Y., Yoon H.J., Kim E.S., Lee Y., Choi S.-H., Kim N.J., Woo J.H., Kim Y.S.
Antimicrob. Agents Chemother. 49:2522-2524(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[21]Johnson T.J., Nolan L.K.
Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: A2363.
[22]Sundstroem L., Swedberg G., Skoeld O.
Submitted (MAR-1991) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-120.
Transposon: Tn5086.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X12868 Genomic DNA. Translation: CAA31358.1.
M95287 Genomic DNA. Translation: AAB59087.1.
U42226 Genomic DNA. Translation: AAC53726.1.
X15024 Genomic DNA. Translation: CAA33123.1.
A07921 Unassigned DNA. Translation: CAA00729.1.
X12870 Genomic DNA. Translation: CAA31364.1.
AF047479 Genomic DNA. Translation: AAC14739.1.
L06418 Genomic DNA. Translation: AAA92747.1.
L06418 Genomic DNA. Translation: AAA92751.3.
U04277 Genomic DNA. Translation: AAB60179.1.
AF071413 Genomic DNA. Translation: AAC33914.1.
AF205943 Genomic DNA. Translation: AAG45723.1.
U12441 Genomic DNA. Translation: AAK95985.1.
AB061794 Genomic DNA. Translation: BAB72156.1.
AF174129 Genomic DNA. Translation: AAK60189.2.
AF550679 Genomic DNA. Translation: AAO49595.1.
AY259085 Genomic DNA. Translation: AAP20908.1.
AY259085 Genomic DNA. Translation: AAP20913.1.
AY259086 Genomic DNA. Translation: AAP20924.1.
AY259086 Genomic DNA. Translation: AAP20929.1.
AY224185 Genomic DNA. Translation: AAP51287.1.
AY260546 Genomic DNA. Translation: AAP22979.1.
AY339625 Genomic DNA. Translation: AAQ16671.1.
L06822 Genomic DNA. Translation: AAW29415.1.
L06822 Genomic DNA. Translation: AAW29418.1.
AY655485 Genomic DNA. Translation: AAW31097.1.
AY878717 Genomic DNA. Translation: AAX18266.1.
AY878717 Genomic DNA. Translation: AAX18269.1.
AY878718 Genomic DNA. Translation: AAX18276.1.
AY878718 Genomic DNA. Translation: AAX18279.1.
AY214164 Genomic DNA. Translation: AAP51280.1.
X58425 Genomic DNA. Translation: CAA41328.1.
PIRSYECOG. A60174.
T45123.
RefSeqNP_863000.1. NC_004998.1.
YP_001096354.1. NC_009132.1.
YP_001096411.1. NC_009133.1.
YP_002891164.1. NC_012690.1.
YP_002894492.1. NC_012692.1.
YP_003937681.1. NC_014615.1.
YP_004558213.1. NC_015599.1.
YP_006902980.1. NC_019037.1.
YP_006903340.1. NC_019045.2.
YP_006939957.1. NC_018994.1.
YP_006952407.1. NC_019062.1.
YP_006952437.1. NC_019063.1.
YP_006952978.1. NC_019066.1.
YP_006953197.1. NC_019069.1.
YP_006953612.1. NC_019081.1.
YP_006953620.1. NC_019082.1.
YP_008574825.1. NC_022374.1.
YP_190215.1. NC_006671.1.
YP_724471.1. NC_007682.3.

3D structure databases

ProteinModelPortalP0C002.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP0C002.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID10752055.
13876924.
13900729.
13903449.
13905341.
13905373.
13905882.
13906105.
13906547.
13906557.
1446559.
17035641.
3244835.
4246764.
4924679.
4924736.
7872397.
7872757.
9846098.

Phylogenomic databases

ProtClustDBPRK13753.

Enzyme and pathway databases

UniPathwayUPA00077; UER00156.

Family and domain databases

Gene3D3.20.20.20. 1 hit.
InterProIPR006390. DHP_synth.
IPR011005. Dihydropteroate_synth-like.
IPR000489. Pterin-binding.
[Graphical view]
PfamPF00809. Pterin_bind. 1 hit.
[Graphical view]
SUPFAMSSF51717. SSF51717. 1 hit.
TIGRFAMsTIGR01496. DHPS. 1 hit.
PROSITEPS00792. DHPS_1. 1 hit.
PS00793. DHPS_2. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHP1_ECOLX
AccessionPrimary (citable) accession number: P0C002
Secondary accession number(s): P11744, Q79LJ7, Q93K51
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: January 22, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways