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Protein

Dihydropteroate synthase type-1

Gene

sulI

Organism
Escherichia coli
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the condensation of para-aminobenzoate (pABA) with 6-hydroxymethyl-7,8-dihydropterin diphosphate (DHPt-PP) to form 7,8-dihydropteroate (H2Pte), the immediate precursor of folate derivatives (By similarity). It is implicated in resistance to sulfonamide. The type II enzyme is stable whereas type I DHPS loses its activity rapidly.By similarity

Miscellaneous

The sulI gene is located on various large self-transmissible resistance plasmids and on transposons related to Tn21.
The plasmid pDGO100 contains two copies of the sulI gene.

Catalytic activityi

6-hydroxymethyl-7,8-dihydropterin diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.By similarity

Cofactori

Mg2+By similarity

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Dihydropteroate synthase (sul1), Dihydropteroate synthase (folP), Dihydropteroate synthase (sul3), Dihydropteroate synthase, Dihydropteroate synthase (folP), Dihydropteroate synthase (CWS33_26340), Dihydropteroate synthase (folP), Dihydropteroate synthase, Dihydropteroate synthase (sul2), Dihydropteroate synthase (folP), Dihydropteroate synthase (folP), Dihydropteroate synthase (SulI), Dihydropteroate synthase (folP), Dihydropteroate synthase (BK383_15895), Dihydropteroate synthase (CRT55_03920), Dihydropteroate synthase (sul1), Dihydropteroate synthase (folP), Dihydropteroate synthase (BSR05_04210), Dihydropteroate synthase (AW059_15240), Dihydropteroate synthase (folP), Dihydropteroate synthase type-1 (sulI), Dihydropteroate synthase (CRM83_20785), Dihydropteroate synthase (folP), Dihydropteroate synthase, Dihydropteroate synthase (sul2), Dihydropteroate synthase (sul1), Dihydropteroate synthase (folP), Dihydropteroate synthase (folP), Dihydropteroate synthase (C2U48_30365), Dihydropteroate synthase (BMT49_25665), Dihydropteroate synthase (sul1), Dihydropteroate synthase (BK375_28220), Dihydropteroate synthase (sul2), Dihydropteroate synthase (SM09_03452), Dihydropteroate synthase (folP_3), Dihydropteroate synthase (folP), Dihydropteroate synthase (APU18_05315), Dihydropteroate synthase (C1I39_27460), Dihydropteroate synthase (sul3), Dihydropteroate synthase (folP), Dihydropteroate synthase (sul3), Dihydropteroate synthase (A9819_18240), Dihydropteroate synthase (sulI), Dihydropteroate synthase (ACN81_18515), Dihydropteroate synthase (sul2), Dihydropteroate synthase (folP), Dihydropteroate synthase (folP), Dihydropteroate synthase (sul1), Dihydropteroate synthase (folP), Dihydropteroate synthase (BK400_32640), Dihydropteroate synthase (C2U48_30480), Dihydropteroate synthase type-2 (sulII), Dihydropteroate synthase (B634_00023), Dihydropteroate synthase (CR534_03110), Dihydropteroate synthase (BXT93_15530), Dihydropteroate synthase (BMR14_26270), Dihydropteroate synthase (sul1), Dihydropteroate synthase (sulII), Dihydropteroate synthase, Dihydropteroate synthase (BMR22_19505), Dihydropteroate synthase (sulII), Dihydropteroate synthase (sul2)
  2. no protein annotated in this organism
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi9MagnesiumBy similarity1
Binding sitei826-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei1016-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei1736-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei2126-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAntibiotic resistance, Folate biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00077; UER00156

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydropteroate synthase type-1 (EC:2.5.1.15)
Alternative name(s):
Dihydropteroate pyrophosphorylase type I
Dihydropteroate synthase type I
Short name:
DHPS
Gene namesi
Name:sulI
Synonyms:sul1
Encoded oniPlasmid IncFII R100 (NR1)1 Publication
Plasmid IncW R3882 Publications
Plasmid pLMO201 Publication
Plasmid pLMO271 Publication
Plasmid IncN R462 Publications
Plasmid pDGO1001 Publication
Plasmid R6-51 Publication
Plasmid pDGO1011 Publication
Plasmid IncFII NR791 Publication
Plasmid IncW pSa3 Publications
Plasmid IncP-beta R7511 Publication
Plasmid pCMXR11 Publication
Plasmid pMSP0711 Publication
Plasmid p1658/971 Publication
Plasmid pHSH11 Publication
Plasmid pHSH21 Publication
Plasmid pAK331 Publication
Plasmid p5411 Publication
Plasmid pQR-11 Publication
Plasmid pKO561 Publication
Plasmid pKO971 Publication
Plasmid pAPEC-O2-R1 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Pathology & Biotechi

Chemistry databases

DrugBankiDB00634 Sulfacetamide

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001682011 – 279Dihydropteroate synthase type-1Add BLAST279

Interactioni

Subunit structurei

Homodimer or homotrimer.

Structurei

3D structure databases

ProteinModelPortaliP0C002
SMRiP0C002
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 258Pterin-bindingPROSITE-ProRule annotationAdd BLAST258

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni246 – 2486-hydroxymethyl-7,8-dihydropterin diphosphate bindingBy similarity3

Sequence similaritiesi

Belongs to the DHPS family.Curated

Family and domain databases

CDDicd00739 DHPS, 1 hit
Gene3Di3.20.20.20, 1 hit
InterProiView protein in InterPro
IPR006390 DHP_synth
IPR011005 Dihydropteroate_synth-like
IPR000489 Pterin-binding_dom
PfamiView protein in Pfam
PF00809 Pterin_bind, 1 hit
SUPFAMiSSF51717 SSF51717, 1 hit
TIGRFAMsiTIGR01496 DHPS, 1 hit
PROSITEiView protein in PROSITE
PS00792 DHPS_1, 1 hit
PS00793 DHPS_2, 1 hit
PS50972 PTERIN_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

P0C002-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTVFGILNL TEDSFFDESR RLDPAGAVTA AIEMLRVGSD VVDVGPAASH
60 70 80 90 100
PDARPVSPAD EIRRIAPLLD ALSDQMHRVS IDSFQPETQR YALKRGVGYL
110 120 130 140 150
NDIQGFPDPA LYPDIAEADC RLVVMHSAQR DGIATRTGHL RPEDALDEIV
160 170 180 190 200
RFFEARVSAL RRSGVAADRL ILDPGMGFFL SPAPETSLHV LSNLQKLKSA
210 220 230 240 250
LGLPLLVSVS RKSFLGATVG LPVKDLGPAS LAAELHAIGN GADYVRTHAP
260 270
GDLRSAITFS ETLAKFRSRD ARDRGLDHA
Length:279
Mass (Da):30,126
Last modified:June 7, 2005 - v1
Checksum:i13EE36C6E3FBDF63
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti259F → I in CAA00729 (PubMed:2662140).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12868 Genomic DNA Translation: CAA31358.1
M95287 Genomic DNA Translation: AAB59087.1
U42226 Genomic DNA Translation: AAC53726.1
X15024 Genomic DNA Translation: CAA33123.1
A07921 Unassigned DNA Translation: CAA00729.1
X12870 Genomic DNA Translation: CAA31364.1
AF047479 Genomic DNA Translation: AAC14739.1
L06418 Genomic DNA Translation: AAA92747.1
L06418 Genomic DNA Translation: AAA92751.3
U04277 Genomic DNA Translation: AAB60179.1
AF071413 Genomic DNA Translation: AAC33914.1
AF205943 Genomic DNA Translation: AAG45723.1
U12441 Genomic DNA Translation: AAK95985.1
AB061794 Genomic DNA Translation: BAB72156.1
AF174129 Genomic DNA Translation: AAK60189.2
AF550679 Genomic DNA Translation: AAO49595.1
AY259085 Genomic DNA Translation: AAP20908.1
AY259085 Genomic DNA Translation: AAP20913.1
AY259086 Genomic DNA Translation: AAP20924.1
AY259086 Genomic DNA Translation: AAP20929.1
AY224185 Genomic DNA Translation: AAP51287.1
AY260546 Genomic DNA Translation: AAP22979.1
AY339625 Genomic DNA Translation: AAQ16671.1
L06822 Genomic DNA Translation: AAW29415.1
L06822 Genomic DNA Translation: AAW29418.1
AY655485 Genomic DNA Translation: AAW31097.1
AY878717 Genomic DNA Translation: AAX18266.1
AY878717 Genomic DNA Translation: AAX18269.1
AY878718 Genomic DNA Translation: AAX18276.1
AY878718 Genomic DNA Translation: AAX18279.1
AY214164 Genomic DNA Translation: AAP51280.1
X58425 Genomic DNA Translation: CAA41328.1
PIRiA60174 SYECOG
T45123
RefSeqiNP_863000.1, NC_004998.1
WP_000259031.1, NZ_PUDW01000050.1
YP_001096354.1, NC_009132.1
YP_001096411.1, NC_009133.1
YP_002891164.1, NC_012690.1
YP_002894492.1, NC_012692.1
YP_003937681.1, NC_014615.1
YP_004558213.1, NC_015599.1
YP_006902980.1, NC_019037.1
YP_006903340.1, NC_019045.2
YP_006939957.1, NC_018994.1
YP_006952407.1, NC_019062.1
YP_006952437.1, NC_019063.1
YP_006952978.1, NC_019066.1
YP_006953197.1, NC_019069.1
YP_006953612.1, NC_019081.1
YP_006953620.1, NC_019082.1
YP_008574825.1, NC_022374.1
YP_009023104.1, NC_023909.1
YP_009060115.1, NC_024956.1
YP_009061085.1, NC_024975.1
YP_009068292.1, NC_025139.1
YP_009070794.1, NC_025175.1
YP_009182147.1, NC_028464.1
YP_190215.1, NC_006671.1
YP_724471.1, NC_007682.3

Genome annotation databases

GeneIDi10752055
13876924
13900729
13903449
13905341
13905373
13905882
13906105
13906547
13906557
1446559
17035641
18983605
20466880
20468108
20491422
20492862
29200589
3244835
4246764
4924679
4924736
4999104
7872397
7872757
9846098

Similar proteinsi

Entry informationi

Entry nameiDHP1_ECOLX
AccessioniPrimary (citable) accession number: P0C002
Secondary accession number(s): P11744, Q79LJ7, Q93K51
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: May 23, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid, Transposable element

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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