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Protein

Dihydropteroate synthase type-1

Gene

sulI

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the condensation of para-aminobenzoate (pABA) with 6-hydroxymethyl-7,8-dihydropterin diphosphate (DHPt-PP) to form 7,8-dihydropteroate (H2Pte), the immediate precursor of folate derivatives (By similarity). It is implicated in resistance to sulfonamide. The type II enzyme is stable whereas type I DHPS loses its activity rapidly.By similarity

Miscellaneous

The sulI gene is located on various large self-transmissible resistance plasmids and on transposons related to Tn21.
The plasmid pDGO100 contains two copies of the sulI gene.

Catalytic activityi

6-hydroxymethyl-7,8-dihydropterin diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.By similarity

Cofactori

Mg2+By similarity

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Dihydropteroate synthase (sul1), Dihydropteroate synthase (folP), Dihydropteroate synthase (sul3), Dihydropteroate synthase, Dihydropteroate synthase (folP), Dihydropteroate synthase (folP), Dihydropteroate synthase, Dihydropteroate synthase (sul2), Dihydropteroate synthase (folP), Dihydropteroate synthase (BMT94_12660), Dihydropteroate synthase (folP), Dihydropteroate synthase (SulI), Dihydropteroate synthase (BK383_15895), Dihydropteroate synthase (CI672_24040), Dihydropteroate synthase (sul1), Dihydropteroate synthase (folP), Dihydropteroate synthase (AW059_15240), Dihydropteroate synthase (folP), Dihydropteroate synthase (folP), Dihydropteroate synthase type-1 (sulI), Dihydropteroate synthase, Dihydropteroate synthase, Dihydropteroate synthase (sul2), Dihydropteroate synthase (folP), Dihydropteroate synthase (folP), Dihydropteroate synthase (sul1), Dihydropteroate synthase (BMT49_25665), Dihydropteroate synthase (BK375_28220), Dihydropteroate synthase (sul2), Dihydropteroate synthase (SM09_03452), Dihydropteroate synthase (folP), Dihydropteroate synthase (APU18_05315), Dihydropteroate synthase (sul3), Dihydropteroate synthase (folP), Dihydropteroate synthase (sul3), Dihydropteroate synthase (sulI), Dihydropteroate synthase (ACN81_18515), Dihydropteroate synthase (sul2), Dihydropteroate synthase (RW82_03360), Dihydropteroate synthase (folP), Dihydropteroate synthase (folP), Dihydropteroate synthase (sul1), Dihydropteroate synthase (BK400_32640), Dihydropteroate synthase type-2 (sulII), Dihydropteroate synthase (B634_00023), Dihydropteroate synthase (BXT93_15530), Dihydropteroate synthase (sul1), Dihydropteroate synthase (sulII), Dihydropteroate synthase, Dihydropteroate synthase (RX20_04412), Dihydropteroate synthase (sulII), Dihydropteroate synthase (sul2)
  2. no protein annotated in this organism
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi9MagnesiumBy similarity1
Binding sitei826-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei1016-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei1736-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1
Binding sitei2126-hydroxymethyl-7,8-dihydropterin diphosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAntibiotic resistance, Folate biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00077; UER00156.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydropteroate synthase type-1 (EC:2.5.1.15)
Alternative name(s):
Dihydropteroate pyrophosphorylase type I
Dihydropteroate synthase type I
Short name:
DHPS
Gene namesi
Name:sulI
Synonyms:sul1
Encoded oniPlasmid IncFII R100 (NR1)1 Publication
Plasmid IncW R3882 Publications
Plasmid pLMO201 Publication
Plasmid pLMO271 Publication
Plasmid IncN R462 Publications
Plasmid pDGO1001 Publication
Plasmid R6-51 Publication
Plasmid pDGO1011 Publication
Plasmid IncFII NR791 Publication
Plasmid IncW pSa3 Publications
Plasmid IncP-beta R7511 Publication
Plasmid pCMXR11 Publication
Plasmid pMSP0711 Publication
Plasmid p1658/971 Publication
Plasmid pHSH11 Publication
Plasmid pHSH21 Publication
Plasmid pAK331 Publication
Plasmid p5411 Publication
Plasmid pQR-11 Publication
Plasmid pKO561 Publication
Plasmid pKO971 Publication
Plasmid pAPEC-O2-R1 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Pathology & Biotechi

Chemistry databases

DrugBankiDB00634. Sulfacetamide.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001682011 – 279Dihydropteroate synthase type-1Add BLAST279

Interactioni

Subunit structurei

Homodimer or homotrimer.

Structurei

3D structure databases

ProteinModelPortaliP0C002.
SMRiP0C002.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 258Pterin-bindingPROSITE-ProRule annotationAdd BLAST258

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni246 – 2486-hydroxymethyl-7,8-dihydropterin diphosphate bindingBy similarity3

Sequence similaritiesi

Belongs to the DHPS family.Curated

Family and domain databases

CDDicd00739. DHPS. 1 hit.
Gene3Di3.20.20.20. 1 hit.
InterProiView protein in InterPro
IPR006390. DHP_synth.
IPR011005. Dihydropteroate_synth-like.
IPR000489. Pterin-binding_dom.
PfamiView protein in Pfam
PF00809. Pterin_bind. 1 hit.
SUPFAMiSSF51717. SSF51717. 1 hit.
TIGRFAMsiTIGR01496. DHPS. 1 hit.
PROSITEiView protein in PROSITE
PS00792. DHPS_1. 1 hit.
PS00793. DHPS_2. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.

Sequencei

Sequence statusi: Complete.

P0C002-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTVFGILNL TEDSFFDESR RLDPAGAVTA AIEMLRVGSD VVDVGPAASH
60 70 80 90 100
PDARPVSPAD EIRRIAPLLD ALSDQMHRVS IDSFQPETQR YALKRGVGYL
110 120 130 140 150
NDIQGFPDPA LYPDIAEADC RLVVMHSAQR DGIATRTGHL RPEDALDEIV
160 170 180 190 200
RFFEARVSAL RRSGVAADRL ILDPGMGFFL SPAPETSLHV LSNLQKLKSA
210 220 230 240 250
LGLPLLVSVS RKSFLGATVG LPVKDLGPAS LAAELHAIGN GADYVRTHAP
260 270
GDLRSAITFS ETLAKFRSRD ARDRGLDHA
Length:279
Mass (Da):30,126
Last modified:June 7, 2005 - v1
Checksum:i13EE36C6E3FBDF63
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti259F → I in CAA00729 (PubMed:2662140).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12868 Genomic DNA. Translation: CAA31358.1.
M95287 Genomic DNA. Translation: AAB59087.1.
U42226 Genomic DNA. Translation: AAC53726.1.
X15024 Genomic DNA. Translation: CAA33123.1.
A07921 Unassigned DNA. Translation: CAA00729.1.
X12870 Genomic DNA. Translation: CAA31364.1.
AF047479 Genomic DNA. Translation: AAC14739.1.
L06418 Genomic DNA. Translation: AAA92747.1.
L06418 Genomic DNA. Translation: AAA92751.3.
U04277 Genomic DNA. Translation: AAB60179.1.
AF071413 Genomic DNA. Translation: AAC33914.1.
AF205943 Genomic DNA. Translation: AAG45723.1.
U12441 Genomic DNA. Translation: AAK95985.1.
AB061794 Genomic DNA. Translation: BAB72156.1.
AF174129 Genomic DNA. Translation: AAK60189.2.
AF550679 Genomic DNA. Translation: AAO49595.1.
AY259085 Genomic DNA. Translation: AAP20908.1.
AY259085 Genomic DNA. Translation: AAP20913.1.
AY259086 Genomic DNA. Translation: AAP20924.1.
AY259086 Genomic DNA. Translation: AAP20929.1.
AY224185 Genomic DNA. Translation: AAP51287.1.
AY260546 Genomic DNA. Translation: AAP22979.1.
AY339625 Genomic DNA. Translation: AAQ16671.1.
L06822 Genomic DNA. Translation: AAW29415.1.
L06822 Genomic DNA. Translation: AAW29418.1.
AY655485 Genomic DNA. Translation: AAW31097.1.
AY878717 Genomic DNA. Translation: AAX18266.1.
AY878717 Genomic DNA. Translation: AAX18269.1.
AY878718 Genomic DNA. Translation: AAX18276.1.
AY878718 Genomic DNA. Translation: AAX18279.1.
AY214164 Genomic DNA. Translation: AAP51280.1.
X58425 Genomic DNA. Translation: CAA41328.1.
PIRiA60174. SYECOG.
T45123.
RefSeqiNP_863000.1. NC_004998.1.
WP_000259031.1. NZ_NQDT01000115.1.
YP_001096354.1. NC_009132.1.
YP_001096411.1. NC_009133.1.
YP_002891164.1. NC_012690.1.
YP_002894492.1. NC_012692.1.
YP_003937681.1. NC_014615.1.
YP_004558213.1. NC_015599.1.
YP_006902980.1. NC_019037.1.
YP_006903340.1. NC_019045.2.
YP_006939957.1. NC_018994.1.
YP_006952407.1. NC_019062.1.
YP_006952437.1. NC_019063.1.
YP_006952978.1. NC_019066.1.
YP_006953197.1. NC_019069.1.
YP_006953612.1. NC_019081.1.
YP_006953620.1. NC_019082.1.
YP_008574825.1. NC_022374.1.
YP_009023104.1. NC_023909.1.
YP_009060115.1. NC_024956.1.
YP_009061085.1. NC_024975.1.
YP_009068292.1. NC_025139.1.
YP_009070794.1. NC_025175.1.
YP_009182147.1. NC_028464.1.
YP_190215.1. NC_006671.1.
YP_724471.1. NC_007682.3.

Genome annotation databases

GeneIDi10752055.
13876924.
13900729.
13903449.
13905341.
13905373.
13905882.
13906105.
13906547.
13906557.
1446559.
17035641.
18983605.
20466880.
20468108.
20491422.
20492862.
29200589.
3244835.
4246764.
4924679.
4924736.
4999104.
7872397.
7872757.
9846098.

Similar proteinsi

Entry informationi

Entry nameiDHP1_ECOLX
AccessioniPrimary (citable) accession number: P0C002
Secondary accession number(s): P11744, Q79LJ7, Q93K51
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: November 22, 2017
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid, Transposable element

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families