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Protein

Uracil permease

Gene

uraA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transport of uracil in the cell.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei73 – 731Uracil; via amide nitrogen1 Publication
Binding sitei241 – 2411Uracil1 Publication
Binding sitei289 – 2891Uracil; via amide nitrogen1 Publication
Binding sitei290 – 2901Uracil1 Publication

GO - Molecular functioni

  • uracil transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • uracil transmembrane transport Source: GOC
  • uracil transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:URAA-MONOMER.
ECOL316407:JW2482-MONOMER.
MetaCyc:URAA-MONOMER.

Protein family/group databases

TCDBi2.A.40.1.1. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Uracil permease
Alternative name(s):
Uracil transporter
Gene namesi
Name:uraA
Ordered Locus Names:b2497, JW2482
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12129. uraA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1313Cytoplasmic1 PublicationAdd
BLAST
Transmembranei14 – 3724Helical1 PublicationAdd
BLAST
Topological domaini38 – 414Periplasmic1 Publication
Transmembranei42 – 6120Helical1 PublicationAdd
BLAST
Topological domaini62 – 643Cytoplasmic1 Publication
Transmembranei65 – 8117Discontinuously helical1 PublicationAdd
BLAST
Topological domaini82 – 898Periplasmic1 Publication
Transmembranei90 – 11021Helical1 PublicationAdd
BLAST
Topological domaini111 – 12212Cytoplasmic1 PublicationAdd
BLAST
Transmembranei123 – 14422Helical1 PublicationAdd
BLAST
Topological domaini145 – 15511Periplasmic1 PublicationAdd
BLAST
Transmembranei156 – 17116Helical1 PublicationAdd
BLAST
Topological domaini172 – 1787Cytoplasmic1 Publication
Transmembranei179 – 19921Helical1 PublicationAdd
BLAST
Topological domaini200 – 22425Periplasmic1 PublicationAdd
BLAST
Transmembranei225 – 24824Helical1 PublicationAdd
BLAST
Topological domaini249 – 26113Cytoplasmic1 PublicationAdd
BLAST
Transmembranei262 – 28120Helical1 PublicationAdd
BLAST
Transmembranei282 – 29817Discontinuously helical1 PublicationAdd
BLAST
Topological domaini299 – 3013Cytoplasmic1 Publication
Transmembranei302 – 31918Helical1 PublicationAdd
BLAST
Topological domaini320 – 33213Periplasmic1 PublicationAdd
BLAST
Transmembranei333 – 35422Helical1 PublicationAdd
BLAST
Topological domaini355 – 36511Cytoplasmic1 PublicationAdd
BLAST
Intramembranei366 – 40136Discontinuously helical1 PublicationAdd
BLAST
Topological domaini402 – 42928Cytoplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB
  • integral component of plasma membrane Source: EcoCyc
  • membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi73 – 731F → A: Slightly decreased uracil uptake. 1 Publication
Mutagenesisi241 – 2411E → A: Abolishes uracil uptake. Abolishes uracil binding. 1 Publication
Mutagenesisi245 – 2451H → A: Abolishes uracil uptake. Abolishes uracil binding. 1 Publication
Mutagenesisi288 – 2881Y → A: No effect on uracil uptake. 1 Publication
Mutagenesisi290 – 2901E → A: Abolishes uracil uptake. Abolishes uracil binding. 1 Publication
Mutagenesisi291 – 2911N → A: Slightly decreased uracil uptake. 1 Publication
Mutagenesisi342 – 3421Y → A: Slightly decreased uracil uptake. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 429429Uracil permeasePRO_0000165958Add
BLAST

Proteomic databases

PaxDbiP0AGM7.

Interactioni

Protein-protein interaction databases

BioGridi4261437. 8 interactions.
DIPiDIP-11094N.
IntActiP0AGM7. 1 interaction.
MINTiMINT-1238837.
STRINGi511145.b2497.

Structurei

Secondary structure

1
429
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi14 – 3724Combined sources
Turni38 – 403Combined sources
Helixi43 – 6018Combined sources
Turni61 – 633Combined sources
Beta strandi69 – 713Combined sources
Helixi73 – 753Combined sources
Helixi76 – 827Combined sources
Helixi83 – 853Combined sources
Helixi87 – 10923Combined sources
Helixi114 – 1196Combined sources
Helixi122 – 14322Combined sources
Helixi156 – 17419Combined sources
Beta strandi175 – 1773Combined sources
Turni178 – 1825Combined sources
Helixi183 – 20119Combined sources
Helixi206 – 2094Combined sources
Helixi225 – 2317Combined sources
Helixi233 – 25321Combined sources
Helixi258 – 2625Combined sources
Helixi263 – 28018Combined sources
Beta strandi285 – 2873Combined sources
Helixi289 – 29810Combined sources
Helixi303 – 31614Combined sources
Helixi320 – 3267Combined sources
Helixi331 – 35525Combined sources
Helixi363 – 37816Combined sources
Beta strandi384 – 3863Combined sources
Helixi391 – 40616Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3QE7X-ray2.78A1-429[»]
ProteinModelPortaliP0AGM7.
SMRiP0AGM7. Positions 4-410.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C2W. Bacteria.
COG2233. LUCA.
HOGENOMiHOG000038200.
InParanoidiP0AGM7.
KOiK02824.
OMAiNIEFEGM.
OrthoDBiEOG6Z3KKZ.
PhylomeDBiP0AGM7.

Family and domain databases

InterProiIPR006042. Xan_ur_permease.
IPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERiPTHR11119. PTHR11119. 1 hit.
PfamiPF00860. Xan_ur_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00801. ncs2. 1 hit.
PROSITEiPS01116. XANTH_URACIL_PERMASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AGM7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRRAIGVSE RPPLLQTIPL SLQHLFAMFG ATVLVPVLFH INPATVLLFN
60 70 80 90 100
GIGTLLYLFI CKGKIPAYLG SSFAFISPVL LLLPLGYEVA LGGFIMCGVL
110 120 130 140 150
FCLVSFIVKK AGTGWLDVLF PPAAMGAIVA VIGLELAGVA AGMAGLLPAE
160 170 180 190 200
GQTPDSKTII ISITTLAVTV LGSVLFRGFL AIIPILIGVL VGYALSFAMG
210 220 230 240 250
IVDTTPIINA HWFALPTLYT PRFEWFAILT ILPAALVVIA EHVGHLVVTA
260 270 280 290 300
NIVKKDLLRD PGLHRSMFAN GLSTVISGFF GSTPNTTYGE NIGVMAITRV
310 320 330 340 350
YSTWVIGGAA IFAILLSCVG KLAAAIQMIP LPVMGGVSLL LYGVIGASGI
360 370 380 390 400
RVLIESKVDY NKAQNLILTS VILIIGVSGA KVNIGAAELK GMALATIVGI
410 420
GLSLIFKLIS VLRPEEVVLD AEDADITDK
Length:429
Mass (Da):45,060
Last modified:December 20, 2005 - v1
Checksum:i18045190C960C674
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73586 Genomic DNA. Translation: CAA51992.1.
U00096 Genomic DNA. Translation: AAC75550.1.
AP009048 Genomic DNA. Translation: BAA16385.1.
PIRiA56265.
RefSeqiNP_416992.1. NC_000913.3.
WP_000198328.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75550; AAC75550; b2497.
BAA16385; BAA16385; BAA16385.
GeneIDi946978.
KEGGiecj:JW2482.
eco:b2497.
PATRICi32120381. VBIEscCol129921_2593.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73586 Genomic DNA. Translation: CAA51992.1.
U00096 Genomic DNA. Translation: AAC75550.1.
AP009048 Genomic DNA. Translation: BAA16385.1.
PIRiA56265.
RefSeqiNP_416992.1. NC_000913.3.
WP_000198328.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3QE7X-ray2.78A1-429[»]
ProteinModelPortaliP0AGM7.
SMRiP0AGM7. Positions 4-410.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261437. 8 interactions.
DIPiDIP-11094N.
IntActiP0AGM7. 1 interaction.
MINTiMINT-1238837.
STRINGi511145.b2497.

Protein family/group databases

TCDBi2.A.40.1.1. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Proteomic databases

PaxDbiP0AGM7.

Protocols and materials databases

DNASUi946978.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75550; AAC75550; b2497.
BAA16385; BAA16385; BAA16385.
GeneIDi946978.
KEGGiecj:JW2482.
eco:b2497.
PATRICi32120381. VBIEscCol129921_2593.

Organism-specific databases

EchoBASEiEB2050.
EcoGeneiEG12129. uraA.

Phylogenomic databases

eggNOGiENOG4105C2W. Bacteria.
COG2233. LUCA.
HOGENOMiHOG000038200.
InParanoidiP0AGM7.
KOiK02824.
OMAiNIEFEGM.
OrthoDBiEOG6Z3KKZ.
PhylomeDBiP0AGM7.

Enzyme and pathway databases

BioCyciEcoCyc:URAA-MONOMER.
ECOL316407:JW2482-MONOMER.
MetaCyc:URAA-MONOMER.

Miscellaneous databases

PROiP0AGM7.

Family and domain databases

InterProiIPR006042. Xan_ur_permease.
IPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERiPTHR11119. PTHR11119. 1 hit.
PfamiPF00860. Xan_ur_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00801. ncs2. 1 hit.
PROSITEiPS01116. XANTH_URACIL_PERMASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Uracil uptake in Escherichia coli K-12: isolation of uraA mutants and cloning of the gene."
    Andersen P.S., Frees D., Fast R., Mygind B.
    J. Bacteriol. 177:2008-2013(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: K12.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.
  6. "Structure and mechanism of the uracil transporter UraA."
    Lu F., Li S., Jiang Y., Jiang J., Fan H., Lu G., Deng D., Dang S., Zhang X., Wang J., Yan N.
    Nature 472:243-246(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.78 ANGSTROMS) IN COMPLEX WITH URACIL, FUNCTION, MUTAGENESIS OF PHE-73; GLU-241; HIS-245; TYR-288; GLU-290; ASN-291 AND TYR-342, MEMBRANE TOPOLOGY.

Entry informationi

Entry nameiURAA_ECOLI
AccessioniPrimary (citable) accession number: P0AGM7
Secondary accession number(s): P33780
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: May 11, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.