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Protein

Uracil permease

Gene

uraA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transport of uracil in the cell.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei73Uracil; via amide nitrogen1 Publication1
Binding sitei241Uracil1 Publication1
Binding sitei289Uracil; via amide nitrogen1 Publication1
Binding sitei290Uracil1 Publication1

GO - Molecular functioni

  • uracil transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • uracil transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:URAA-MONOMER.
ECOL316407:JW2482-MONOMER.
MetaCyc:URAA-MONOMER.

Protein family/group databases

TCDBi2.A.40.1.1. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Uracil permease
Alternative name(s):
Uracil transporter
Gene namesi
Name:uraA
Ordered Locus Names:b2497, JW2482
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12129. uraA.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 13Cytoplasmic1 PublicationAdd BLAST13
Transmembranei14 – 37Helical1 PublicationAdd BLAST24
Topological domaini38 – 41Periplasmic1 Publication4
Transmembranei42 – 61Helical1 PublicationAdd BLAST20
Topological domaini62 – 64Cytoplasmic1 Publication3
Transmembranei65 – 81Discontinuously helical1 PublicationAdd BLAST17
Topological domaini82 – 89Periplasmic1 Publication8
Transmembranei90 – 110Helical1 PublicationAdd BLAST21
Topological domaini111 – 122Cytoplasmic1 PublicationAdd BLAST12
Transmembranei123 – 144Helical1 PublicationAdd BLAST22
Topological domaini145 – 155Periplasmic1 PublicationAdd BLAST11
Transmembranei156 – 171Helical1 PublicationAdd BLAST16
Topological domaini172 – 178Cytoplasmic1 Publication7
Transmembranei179 – 199Helical1 PublicationAdd BLAST21
Topological domaini200 – 224Periplasmic1 PublicationAdd BLAST25
Transmembranei225 – 248Helical1 PublicationAdd BLAST24
Topological domaini249 – 261Cytoplasmic1 PublicationAdd BLAST13
Transmembranei262 – 281Helical1 PublicationAdd BLAST20
Transmembranei282 – 298Discontinuously helical1 PublicationAdd BLAST17
Topological domaini299 – 301Cytoplasmic1 Publication3
Transmembranei302 – 319Helical1 PublicationAdd BLAST18
Topological domaini320 – 332Periplasmic1 PublicationAdd BLAST13
Transmembranei333 – 354Helical1 PublicationAdd BLAST22
Topological domaini355 – 365Cytoplasmic1 PublicationAdd BLAST11
Intramembranei366 – 401Discontinuously helical1 PublicationAdd BLAST36
Topological domaini402 – 429Cytoplasmic1 PublicationAdd BLAST28

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB
  • integral component of plasma membrane Source: EcoCyc
  • membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi73F → A: Slightly decreased uracil uptake. 1 Publication1
Mutagenesisi241E → A: Abolishes uracil uptake. Abolishes uracil binding. 1 Publication1
Mutagenesisi245H → A: Abolishes uracil uptake. Abolishes uracil binding. 1 Publication1
Mutagenesisi288Y → A: No effect on uracil uptake. 1 Publication1
Mutagenesisi290E → A: Abolishes uracil uptake. Abolishes uracil binding. 1 Publication1
Mutagenesisi291N → A: Slightly decreased uracil uptake. 1 Publication1
Mutagenesisi342Y → A: Slightly decreased uracil uptake. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001659581 – 429Uracil permeaseAdd BLAST429

Proteomic databases

PaxDbiP0AGM7.
PRIDEiP0AGM7.

Interactioni

Protein-protein interaction databases

BioGridi4261437. 8 interactors.
DIPiDIP-11094N.
IntActiP0AGM7. 1 interactor.
MINTiMINT-1238837.
STRINGi511145.b2497.

Structurei

Secondary structure

1429
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 37Combined sources24
Turni38 – 40Combined sources3
Helixi43 – 60Combined sources18
Turni61 – 63Combined sources3
Beta strandi69 – 71Combined sources3
Helixi73 – 75Combined sources3
Helixi76 – 82Combined sources7
Helixi83 – 85Combined sources3
Helixi87 – 109Combined sources23
Helixi114 – 119Combined sources6
Helixi122 – 143Combined sources22
Helixi156 – 174Combined sources19
Beta strandi175 – 177Combined sources3
Turni178 – 182Combined sources5
Helixi183 – 201Combined sources19
Helixi206 – 209Combined sources4
Helixi225 – 231Combined sources7
Helixi233 – 253Combined sources21
Helixi258 – 262Combined sources5
Helixi263 – 280Combined sources18
Beta strandi285 – 287Combined sources3
Helixi289 – 298Combined sources10
Helixi303 – 316Combined sources14
Helixi320 – 326Combined sources7
Helixi331 – 355Combined sources25
Helixi363 – 378Combined sources16
Beta strandi384 – 386Combined sources3
Helixi391 – 406Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QE7X-ray2.78A1-429[»]
ProteinModelPortaliP0AGM7.
SMRiP0AGM7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C2W. Bacteria.
COG2233. LUCA.
HOGENOMiHOG000038200.
InParanoidiP0AGM7.
KOiK02824.
OMAiNIEFEGM.
PhylomeDBiP0AGM7.

Family and domain databases

InterProiIPR006042. Xan_ur_permease.
IPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERiPTHR11119. PTHR11119. 1 hit.
PfamiPF00860. Xan_ur_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00801. ncs2. 1 hit.
PROSITEiPS01116. XANTH_URACIL_PERMASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AGM7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRRAIGVSE RPPLLQTIPL SLQHLFAMFG ATVLVPVLFH INPATVLLFN
60 70 80 90 100
GIGTLLYLFI CKGKIPAYLG SSFAFISPVL LLLPLGYEVA LGGFIMCGVL
110 120 130 140 150
FCLVSFIVKK AGTGWLDVLF PPAAMGAIVA VIGLELAGVA AGMAGLLPAE
160 170 180 190 200
GQTPDSKTII ISITTLAVTV LGSVLFRGFL AIIPILIGVL VGYALSFAMG
210 220 230 240 250
IVDTTPIINA HWFALPTLYT PRFEWFAILT ILPAALVVIA EHVGHLVVTA
260 270 280 290 300
NIVKKDLLRD PGLHRSMFAN GLSTVISGFF GSTPNTTYGE NIGVMAITRV
310 320 330 340 350
YSTWVIGGAA IFAILLSCVG KLAAAIQMIP LPVMGGVSLL LYGVIGASGI
360 370 380 390 400
RVLIESKVDY NKAQNLILTS VILIIGVSGA KVNIGAAELK GMALATIVGI
410 420
GLSLIFKLIS VLRPEEVVLD AEDADITDK
Length:429
Mass (Da):45,060
Last modified:December 20, 2005 - v1
Checksum:i18045190C960C674
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73586 Genomic DNA. Translation: CAA51992.1.
U00096 Genomic DNA. Translation: AAC75550.1.
AP009048 Genomic DNA. Translation: BAA16385.1.
PIRiA56265.
RefSeqiNP_416992.1. NC_000913.3.
WP_000198328.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75550; AAC75550; b2497.
BAA16385; BAA16385; BAA16385.
GeneIDi946978.
KEGGiecj:JW2482.
eco:b2497.
PATRICi32120381. VBIEscCol129921_2593.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73586 Genomic DNA. Translation: CAA51992.1.
U00096 Genomic DNA. Translation: AAC75550.1.
AP009048 Genomic DNA. Translation: BAA16385.1.
PIRiA56265.
RefSeqiNP_416992.1. NC_000913.3.
WP_000198328.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QE7X-ray2.78A1-429[»]
ProteinModelPortaliP0AGM7.
SMRiP0AGM7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261437. 8 interactors.
DIPiDIP-11094N.
IntActiP0AGM7. 1 interactor.
MINTiMINT-1238837.
STRINGi511145.b2497.

Protein family/group databases

TCDBi2.A.40.1.1. the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family.

Proteomic databases

PaxDbiP0AGM7.
PRIDEiP0AGM7.

Protocols and materials databases

DNASUi946978.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75550; AAC75550; b2497.
BAA16385; BAA16385; BAA16385.
GeneIDi946978.
KEGGiecj:JW2482.
eco:b2497.
PATRICi32120381. VBIEscCol129921_2593.

Organism-specific databases

EchoBASEiEB2050.
EcoGeneiEG12129. uraA.

Phylogenomic databases

eggNOGiENOG4105C2W. Bacteria.
COG2233. LUCA.
HOGENOMiHOG000038200.
InParanoidiP0AGM7.
KOiK02824.
OMAiNIEFEGM.
PhylomeDBiP0AGM7.

Enzyme and pathway databases

BioCyciEcoCyc:URAA-MONOMER.
ECOL316407:JW2482-MONOMER.
MetaCyc:URAA-MONOMER.

Miscellaneous databases

PROiP0AGM7.

Family and domain databases

InterProiIPR006042. Xan_ur_permease.
IPR006043. Xant/urac/vitC.
[Graphical view]
PANTHERiPTHR11119. PTHR11119. 1 hit.
PfamiPF00860. Xan_ur_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00801. ncs2. 1 hit.
PROSITEiPS01116. XANTH_URACIL_PERMASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiURAA_ECOLI
AccessioniPrimary (citable) accession number: P0AGM7
Secondary accession number(s): P33780
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.