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Reviewed, UniProtKB/Swiss-Prot P0AGL7 (RSME_ECOLI)

Last modified June 16, 2009. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Ribosomal RNA small subunit methyltransferase E
    EC=2.1.1.-
Alternative name(s):
    16S rRNA m3U1498 methyltransferase
Gene names
Name: rsmE
Synonyms: yggJ
Ordered Locus Names: b2946, JW2913
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length243 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit. Ref.6 Ref.7

Subunit structure

Homodimer. Ref.7

Subcellular location

Cytoplasm Probable.

Sequence similarities

Belongs to the RNA methyltransferase rsmE family.

biophysicochemical properties

Kinetic parameters:

KM=2.0 µM for 16S rRNA within 30S ribosomal subunits

KM=26.7 µM for S-adenosyl-L-methionine

pH dependence:

Optimum pH is 7-9.

Ontologies

Keywords
   Biological processrRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processrRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionmethyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 243243Ribosomal RNA small subunit methyltransferase E
PRO_0000176199

Sequences

Sequence LengthMass (Da)Tools
P0AGL7-1 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: 5C4659F5B295E033

FASTA24326,978
        10         20         30         40         50         60 
MRIPRIYHPE PLTSHSHIAL CEDAANHIGR VLRMGPGQAL QLFDGSNQVF DAEITSASKK 

        70         80         90        100        110        120 
SVEVKVLEGQ IDDRESPLHI HLGQVMSRGE KMEFTIQKSI ELGVSLITPL FSERCGVKLD 

       130        140        150        160        170        180 
SERLNKKLQQ WQKIAIAACE QCGRNRVPEI RPAMDLEAWC AEQDEGLKLN LHPRASNSIN 

       190        200        210        220        230        240 
TLPLPVERVR LLIGPEGGLS ADEIAMTARY QFTDILLGPR VLRTETTALT AITALQVRFG 


DLG 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"Location of the endA gene coding for endonuclease I on the physical map of the Escherichia coli K-12 chromosome."
Jekel M., Wackernagel W.
J. Bacteriol. 176:1550-1551(1994) [PubMed: 8113204] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-128.
Strain: K12.
[4]"Complete nucleotide sequence of the E. coli glutathione synthetase gsh-II."
Gushima H., Yasuda S., Soeda E., Yokota M., Kondo M., Kimura A.
Nucleic Acids Res. 12:9299-9307(1984) [PubMed: 6393055] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 127-243.
Strain: B.
[5]Rudd K.E., Baum B.
Unpublished observations (AUG-1994)
Cited for: IDENTIFICATION.
[6]"Identification and characterization of RsmE, the founding member of a new RNA base methyltransferase family."
Basturea G.N., Rudd K.E., Deutscher M.P.
RNA 12:426-434(2006) [PubMed: 16431987] [Abstract]
Cited for: FUNCTION AS METHYLTRANSFERASE.
Strain: K12 / MG1655 / ATCC 47076.
[7]"Substrate specificity and properties of the Escherichia coli 16S rRNA methyltransferase, RsmE."
Basturea G.N., Deutscher M.P.
RNA 13:1969-1976(2007) [PubMed: 17872509] [Abstract]
Cited for: FUNCTION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.

Cross-references

Sequence databases

U28377 Genomic DNA. Translation: AAA69113.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75983.2.
AP009048 Genomic DNA. Translation: BAE77009.1.
X65169 Genomic DNA. No translation available.
X01666 Genomic DNA. No translation available.
RefSeqAP_003503.1.
NP_417421.4.

3D structure databases

HSSPHSSP built from PDB template 1NXZ based on UniProtKB P44627.
ModBaseSearch...

Genome annotation databases

GeneID945816.
GenomeReviewsGene locus JW2913 in contig AP009048_GR.
Gene locus b2946 in contig U00096_GR.
KEGGecj:JW2913.
eco:b2946.

Organism-specific databases

EchoBASEEB2269.
EcoGeneEG12366. rsmE.
CMRSearch...

Phylogenomic databases

HOGENOMP0AGL7.
OMAP0AGL7. RDGEWRA.

Enzyme and pathway databases

BioCycEcoCyc:EG12366-MON.

Family and domain databases

InterProIPR006700. rRNA_ssu_MeTrfase-E.
[Graphical view]
PfamPF04452. Methyltrans_RNA. 1 hit.
[Graphical view]
TIGRFAMsTIGR00046. DUF558_mtase_pred. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRSME_ECOLI
AccessionPrimary (citable) accession number: P0AGL7
Secondary accession number(s): P37912, P76647, Q2M9P7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Uncharacterized protein families (UPF)

List of uncharacterized protein family (UPF) entries

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents