P0AGL7 (RSME_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase E
EC=2.1.1.193

Alternative name(s):
16S rRNA m3U1498 methyltransferase

Gene names

Name:	rsmE
Synonyms:	yggJ
Ordered Locus Names:	b2946, JW2913

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit. Ref.6 Ref.7
Catalytic activity	S-adenosyl-L-methionine + uracil(1498) in 16S rRNA = S-adenosyl-L-homocysteine + N(3)-methyluracil(1498) in 16S rRNA. Ref.6
Subunit structure	Homodimer. Ref.7
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the RNA methyltransferase RsmE family.
Biophysicochemical properties	Kinetic parameters: KM=2.0 µM for 16S rRNA within 30S ribosomal subunits Ref.7 KM=26.7 µM for S-adenosyl-L-methionine pH dependence: Optimum pH is 7-9.
Sequence caution	The sequence AAA69113.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	rRNA base methylation Inferred from mutant phenotype Ref.6. Source: EcoCyc
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	rRNA (uridine-N3-)-methyltransferase activity Inferred from direct assay Ref.6. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 243

243

Ribosomal RNA small subunit methyltransferase E

PRO_0000176199

Secondary structure

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243

Helix Strand Turn

Details...

Beta strand

5 – 7

3

Beta strand

17 – 19

3

Helix

22 – 29

8

Beta strand

39 – 43

5

Beta strand

45 – 57

13

Beta strand

62 – 71

10

Beta strand

77 – 85

9

Beta strand

88 – 90

3

Helix

91 – 101

11

Beta strand

106 – 111

6

Helix

121 – 142

22

Helix

156 – 161

6

Beta strand

165 – 171

7

Beta strand

176 – 178

3

Helix

179 – 181

3

Beta strand

188 – 193

6

Helix

201 – 209

9

Beta strand

213 – 216

4

Helix

224 – 238

15

Sequences

	Sequence		Length	Mass (Da)
	P0AGL7 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 5C4659F5B295E033	FASTA	243	26,978
	10 20 30 40 50 60 MRIPRIYHPE PLTSHSHIAL CEDAANHIGR VLRMGPGQAL QLFDGSNQVF DAEITSASKK 70 80 90 100 110 120 SVEVKVLEGQ IDDRESPLHI HLGQVMSRGE KMEFTIQKSI ELGVSLITPL FSERCGVKLD 130 140 150 160 170 180 SERLNKKLQQ WQKIAIAACE QCGRNRVPEI RPAMDLEAWC AEQDEGLKLN LHPRASNSIN 190 200 210 220 230 240 TLPLPVERVR LLIGPEGGLS ADEIAMTARY QFTDILLGPR VLRTETTALT AITALQVRFG DLG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Location of the endA gene coding for endonuclease I on the physical map of the Escherichia coli K-12 chromosome." Jekel M., Wackernagel W. J. Bacteriol. 176:1550-1551(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-128. Strain: K12.
[4]	"Complete nucleotide sequence of the E. coli glutathione synthetase gsh-II." Gushima H., Yasuda S., Soeda E., Yokota M., Kondo M., Kimura A. Nucleic Acids Res. 12:9299-9307(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 127-243. Strain: B.
[5]	Rudd K.E., Baum B. Unpublished observations (AUG-1994) Cited for: IDENTIFICATION.
[6]	"Identification and characterization of RsmE, the founding member of a new RNA base methyltransferase family." Basturea G.N., Rudd K.E., Deutscher M.P. RNA 12:426-434(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS METHYLTRANSFERASE, CATALYTIC ACTIVITY. Strain: K12 / MG1655 / ATCC 47076.
[7]	"Substrate specificity and properties of the Escherichia coli 16S rRNA methyltransferase, RsmE." Basturea G.N., Deutscher M.P. RNA 13:1969-1976(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U28377 Genomic DNA. Translation: AAA69113.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75983.2.
AP009048 Genomic DNA. Translation: BAE77009.1.
X65169 Genomic DNA. No translation available.
X01666 Genomic DNA. No translation available.

RefSeq

NP_417421.4. NC_000913.2.
YP_491145.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4E8B	X-ray	2.25	A	1-243	[»]

ProteinModelPortal

P0AGL7.

SMR

P0AGL7. Positions 3-243.

ModBase

Search...

Protein-protein interaction databases

IntAct

P0AGL7. 5 interactions.

STRING

511145.b2946.

Proteomic databases

PRIDE

P0AGL7.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC75983; AAC75983; b2946.
BAE77009; BAE77009; BAE77009.

GeneID

12930413.
945816.

KEGG

ecj:Y75_p2876.
eco:b2946.

PATRIC

32121302. VBIEscCol129921_3039.

Organism-specific databases

EchoBASE

EB2269.

EcoGene

EG12366. rsmE.

Phylogenomic databases

eggNOG

COG1385.

HOGENOM

HOG000015265.

KO

K09761.

OMA

MTQHVQG.

ProtClustDB

PRK11713.

Enzyme and pathway databases

BioCyc

EcoCyc:EG12366-MONOMER.
ECOL316407:JW2913-MONOMER.
MetaCyc:EG12366-MONOMER.

Gene expression databases

Genevestigator

P0AGL7.

Family and domain databases

InterPro

IPR015947. PUA-like_domain.
IPR006700. rRNA_ssu_MeTrfase-E.
[Graphical view]

Pfam

PF04452. Methyltrans_RNA. 1 hit.
[Graphical view]

PIRSF

PIRSF015601. MTase_slr0722. 1 hit.

SUPFAM

SSF88697. PUA-like. 1 hit.

TIGRFAMs

TIGR00046. TIGR00046. 1 hit.

ProtoNet

Search...

Entry information

Entry name

RSME_ECOLI

Accession

Primary (citable) accession number: P0AGL7
Secondary accession number(s): P37912, P76647, Q2M9P7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program