Reviewed, UniProtKB/Swiss-Prot P0AGL7 (RSME_ECOLI)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA small subunit methyltransferase E
    EC=2.1.1.-
Alternative name(s):
    16S rRNA m3U1498 methyltransferase

Gene names

Name:	rsmE
Synonyms:	yggJ
Ordered Locus Names:	b2946, JW2913

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit. Ref.6 Ref.7
Subunit structure	Homodimer. Ref.7
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the RNA methyltransferase rsmE family.
biophysicochemical properties	Kinetic parameters: KM=2.0 µM for 16S rRNA within 30S ribosomal subunits KM=26.7 µM for S-adenosyl-L-methionine pH dependence: Optimum pH is 7-9.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	Ribosomal RNA small subunit methyltransferase E		PRO_0000176199

Sequences

	Sequence		Length	Mass (Da)
	P0AGL7-1 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 5C4659F5B295E033	FASTA	243	26,978
	10 20 30 40 50 60 MRIPRIYHPE PLTSHSHIAL CEDAANHIGR VLRMGPGQAL QLFDGSNQVF DAEITSASKK 70 80 90 100 110 120 SVEVKVLEGQ IDDRESPLHI HLGQVMSRGE KMEFTIQKSI ELGVSLITPL FSERCGVKLD 130 140 150 160 170 180 SERLNKKLQQ WQKIAIAACE QCGRNRVPEI RPAMDLEAWC AEQDEGLKLN LHPRASNSIN 190 200 210 220 230 240 TLPLPVERVR LLIGPEGGLS ADEIAMTARY QFTDILLGPR VLRTETTALT AITALQVRFG DLG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Location of the endA gene coding for endonuclease I on the physical map of the Escherichia coli K-12 chromosome." Jekel M., Wackernagel W. J. Bacteriol. 176:1550-1551(1994) [PubMed: 8113204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-128. Strain: K12.
[4]	"Complete nucleotide sequence of the E. coli glutathione synthetase gsh-II." Gushima H., Yasuda S., Soeda E., Yokota M., Kondo M., Kimura A. Nucleic Acids Res. 12:9299-9307(1984) [PubMed: 6393055] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 127-243. Strain: B.
[5]	Rudd K.E., Baum B. Unpublished observations (AUG-1994) Cited for: IDENTIFICATION.
[6]	"Identification and characterization of RsmE, the founding member of a new RNA base methyltransferase family." Basturea G.N., Rudd K.E., Deutscher M.P. RNA 12:426-434(2006) [PubMed: 16431987] [Abstract] Cited for: FUNCTION AS METHYLTRANSFERASE. Strain: K12 / MG1655 / ATCC 47076.
[7]	"Substrate specificity and properties of the Escherichia coli 16S rRNA methyltransferase, RsmE." Basturea G.N., Deutscher M.P. RNA 13:1969-1976(2007) [PubMed: 17872509] [Abstract] Cited for: FUNCTION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U28377 Genomic DNA. Translation: AAA69113.1. Different initiation. U00096 Genomic DNA. Translation: AAC75983.2. AP009048 Genomic DNA. Translation: BAE77009.1. X65169 Genomic DNA. No translation available. X01666 Genomic DNA. No translation available.
RefSeq	AP_003503.1. NP_417421.4.
3D structure databases
HSSP	HSSP built from PDB template 1NXZ based on UniProtKB P44627.
ModBase	Search...
Genome annotation databases
GeneID	945816.
GenomeReviews	Gene locus JW2913 in contig AP009048_GR. Gene locus b2946 in contig U00096_GR.
KEGG	ecj:JW2913. eco:b2946.
Organism-specific databases
EchoBASE	EB2269.
EcoGene	EG12366. rsmE.
CMR	Search...
Phylogenomic databases
HOGENOM	P0AGL7.
OMA	P0AGL7. RDGEWRA.
Enzyme and pathway databases
BioCyc	EcoCyc:EG12366-MON.
Family and domain databases
InterPro	IPR006700. rRNA_ssu_MeTrfase-E. [Graphical view]
Pfam	PF04452. Methyltrans_RNA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00046. DUF558_mtase_pred. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSME_ECOLI

Accession

Primary (citable) accession number: P0AGL7
Secondary accession number(s): P37912, P76647, Q2M9P7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)