ID   RATA_ECOLI              Reviewed;         158 AA.
AC   P0AGL5; P52121;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   24-JAN-2024, entry version 116.
DE   RecName: Full=Ribosome association toxin RatA;
DE   AltName: Full=Toxin RatA;
GN   Name=ratA; Synonyms=pasT, yfjG; OrderedLocusNames=b2619, JW2600;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=7524073; DOI=10.1073/pnas.91.20.9223;
RA   Komine Y., Kitabatake M., Yokogawa T., Nishikawa K., Inokuchi H.;
RT   "A tRNA-like structure is present in 10Sa RNA, a small stable RNA from
RT   Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 91:9223-9227(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA   Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA   Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA   Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA   Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA   Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT   "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT   genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT   its sequence features.";
RL   DNA Res. 4:91-113(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   FUNCTION AS A TOXIN.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=14594833; DOI=10.1128/jb.185.22.6600-6608.2003;
RA   Brown J.M., Shaw K.J.;
RT   "A novel family of Escherichia coli toxin-antitoxin gene pairs.";
RL   J. Bacteriol. 185:6600-6608(2003).
RN   [6]
RP   FUNCTION AS A TOXIN, AND SUBUNIT.
RX   PubMed=21323758; DOI=10.1111/j.1365-2958.2010.07506.x;
RA   Zhang Y., Inouye M.;
RT   "RatA (YfjG), an Escherichia coli toxin, inhibits 70S ribosome association
RT   to block translation initiation.";
RL   Mol. Microbiol. 79:1418-1429(2011).
RN   [7]
RP   ROLE IN STRESS RESPONSE, AND DISRUPTION PHENOTYPE.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=23055930; DOI=10.1371/journal.ppat.1002954;
RA   Norton J.P., Mulvey M.A.;
RT   "Toxin-antitoxin systems are important for niche-specific colonization and
RT   stress resistance of uropathogenic Escherichia coli.";
RL   PLoS Pathog. 8:E1002954-E1002954(2012).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system.
CC       Binds to 50S ribosomal subunits, preventing them from associating with
CC       30S subunits to form 70S ribosomes and reducing polysomes. It does not
CC       cause ribosomes to dissociate however. The antibiotic paromomycin
CC       blocks the anti-association activity of RatA. Overexpression results in
CC       inhibition of growth in liquid cultures, and in a decrease in protein
CC       translation. The other gene of this operon, ratB, is not the cognate
CC       antitoxin in this strain; in CFT073 however it does fulfill this
CC       function.
CC   -!- FUNCTION: Low level expression in a deletion mutant increases
CC       resistance to acidified sodium nitrate which causes nitrosative stress.
CC   -!- SUBUNIT: Associates with 50S ribosomes. {ECO:0000269|PubMed:21323758}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the ratA-ratB operon has no effect on
CC       bacterial persistence on rich medium for this strain, but does affect
CC       bacterial persistence for E.coli strain CFT073.
CC       {ECO:0000269|PubMed:23055930}.
CC   -!- SIMILARITY: Belongs to the ribosome association toxin RatA family.
CC       {ECO:0000305}.
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DR   EMBL; D12501; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; U36840; AAA79789.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC75668.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA16504.1; -; Genomic_DNA.
DR   PIR; T08632; T08632.
DR   RefSeq; NP_417109.1; NC_000913.3.
DR   AlphaFoldDB; P0AGL5; -.
DR   SMR; P0AGL5; -.
DR   BioGRID; 4263441; 17.
DR   IntAct; P0AGL5; 1.
DR   STRING; 511145.b2619; -.
DR   jPOST; P0AGL5; -.
DR   PaxDb; 511145-b2619; -.
DR   EnsemblBacteria; AAC75668; AAC75668; b2619.
DR   GeneID; 945614; -.
DR   KEGG; ecj:JW2600; -.
DR   KEGG; eco:b2619; -.
DR   PATRIC; fig|511145.12.peg.2717; -.
DR   EchoBASE; EB2985; -.
DR   eggNOG; COG2867; Bacteria.
DR   HOGENOM; CLU_079653_3_1_6; -.
DR   InParanoid; P0AGL5; -.
DR   OMA; IDGPFKY; -.
DR   OrthoDB; 9804759at2; -.
DR   PhylomeDB; P0AGL5; -.
DR   BioCyc; EcoCyc:G7358-MONOMER; -.
DR   PRO; PR:P0AGL5; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0043023; F:ribosomal large subunit binding; IDA:EcoCyc.
DR   GO; GO:0048039; F:ubiquinone binding; IEA:InterPro.
DR   GO; GO:0045333; P:cellular respiration; IEA:InterPro.
DR   GO; GO:0045947; P:negative regulation of translational initiation; IMP:EcoCyc.
DR   GO; GO:0006744; P:ubiquinone biosynthetic process; IEA:InterPro.
DR   CDD; cd07813; COQ10p_like; 1.
DR   Gene3D; 3.30.530.20; -; 1.
DR   InterPro; IPR044996; COQ10-like.
DR   InterPro; IPR005031; COQ10_START.
DR   InterPro; IPR023393; START-like_dom_sf.
DR   PANTHER; PTHR12901:SF10; COENZYME Q-BINDING PROTEIN COQ10, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR12901; SPERM PROTEIN HOMOLOG; 1.
DR   Pfam; PF03364; Polyketide_cyc; 1.
DR   SUPFAM; SSF55961; Bet v1-like; 1.
PE   1: Evidence at protein level;
KW   Reference proteome; Stress response; Toxin-antitoxin system.
FT   CHAIN           1..158
FT                   /note="Ribosome association toxin RatA"
FT                   /id="PRO_0000192474"
SQ   SEQUENCE   158 AA;  17727 MW;  68A3A8FBE6AC83D5 CRC64;
     MILFVGFLLM EIVMPQISRT ALVPYSAEQM YQLVNDVQSY PQFLPGCTGS RILESTPGQM
     TAAVDVSKAG ISKTFTTRNQ LTSNQSILMN LVDGPFKKLI GGWKFTPLSQ EACRIEFHLD
     FEFTNKLIEL AFGRVFKELA ANMVQAFTVR AKEVYSAR
//