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Protein

Ribosome association toxin RatA

Gene

ratA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Toxic component of a toxin-antitoxin (TA) module. Binds to 50S ribosomal subunits, preventing them from associating with 30S subunits to form 70S ribosomes and reducing polysomes. It does not cause ribosomes to dissociate however. The antibiotic paromomycin blocks the anti-association activity of RatA. Overexpression results in inhibition of growth in liquid cultures, and in a decrease in protein translation. The other gene of this operon, ratB, is not the cognate antitoxin in this strain; in CFT073 however it does fulfill this function.
Low level expression in a deletion mutant increases resistance to acidified sodium nitrate which causes nitrosative stress.

GO - Molecular functioni

  • ribosomal large subunit binding Source: EcoCyc

GO - Biological processi

  • negative regulation of translational initiation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Toxin

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciEcoCyc:G7358-MONOMER.
ECOL316407:JW2600-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome association toxin RatA
Alternative name(s):
Toxin RatA
Gene namesi
Name:ratA
Synonyms:pasT, yfjG
Ordered Locus Names:b2619, JW2600
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13193. ratA.

Pathology & Biotechi

Disruption phenotypei

Deletion of the ratA-ratB operon has no effect on bacterial persistence on rich medium for this strain, but does affect bacterial persistence for E.coli strain CFT073.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001924741 – 158Ribosome association toxin RatAAdd BLAST158

Proteomic databases

PaxDbiP0AGL5.
PRIDEiP0AGL5.

Interactioni

Subunit structurei

Associates with 50S ribosomes.1 Publication

Protein-protein interaction databases

BioGridi4263441. 11 interactors.
IntActiP0AGL5. 1 interactor.
STRINGi511145.b2619.

Structurei

3D structure databases

ProteinModelPortaliP0AGL5.
SMRiP0AGL5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108Z1B. Bacteria.
COG2867. LUCA.
HOGENOMiHOG000261730.
InParanoidiP0AGL5.
OMAiLREDACK.
PhylomeDBiP0AGL5.

Family and domain databases

Gene3Di3.30.530.20. 1 hit.
InterProiIPR005031. COQ10_START.
IPR023393. START-like_dom.
[Graphical view]
PfamiPF03364. Polyketide_cyc. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AGL5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILFVGFLLM EIVMPQISRT ALVPYSAEQM YQLVNDVQSY PQFLPGCTGS
60 70 80 90 100
RILESTPGQM TAAVDVSKAG ISKTFTTRNQ LTSNQSILMN LVDGPFKKLI
110 120 130 140 150
GGWKFTPLSQ EACRIEFHLD FEFTNKLIEL AFGRVFKELA ANMVQAFTVR

AKEVYSAR
Length:158
Mass (Da):17,727
Last modified:December 20, 2005 - v1
Checksum:i68A3A8FBE6AC83D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12501 Genomic DNA. No translation available.
U36840 Genomic DNA. Translation: AAA79789.1.
U00096 Genomic DNA. Translation: AAC75668.1.
AP009048 Genomic DNA. Translation: BAA16504.1.
PIRiT08632.
RefSeqiNP_417109.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC75668; AAC75668; b2619.
BAA16504; BAA16504; BAA16504.
GeneIDi945614.
KEGGiecj:JW2600.
eco:b2619.
PATRICi32120637. VBIEscCol129921_2717.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12501 Genomic DNA. No translation available.
U36840 Genomic DNA. Translation: AAA79789.1.
U00096 Genomic DNA. Translation: AAC75668.1.
AP009048 Genomic DNA. Translation: BAA16504.1.
PIRiT08632.
RefSeqiNP_417109.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP0AGL5.
SMRiP0AGL5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263441. 11 interactors.
IntActiP0AGL5. 1 interactor.
STRINGi511145.b2619.

Proteomic databases

PaxDbiP0AGL5.
PRIDEiP0AGL5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75668; AAC75668; b2619.
BAA16504; BAA16504; BAA16504.
GeneIDi945614.
KEGGiecj:JW2600.
eco:b2619.
PATRICi32120637. VBIEscCol129921_2717.

Organism-specific databases

EchoBASEiEB2985.
EcoGeneiEG13193. ratA.

Phylogenomic databases

eggNOGiENOG4108Z1B. Bacteria.
COG2867. LUCA.
HOGENOMiHOG000261730.
InParanoidiP0AGL5.
OMAiLREDACK.
PhylomeDBiP0AGL5.

Enzyme and pathway databases

BioCyciEcoCyc:G7358-MONOMER.
ECOL316407:JW2600-MONOMER.

Miscellaneous databases

PROiP0AGL5.

Family and domain databases

Gene3Di3.30.530.20. 1 hit.
InterProiIPR005031. COQ10_START.
IPR023393. START-like_dom.
[Graphical view]
PfamiPF03364. Polyketide_cyc. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRATA_ECOLI
AccessioniPrimary (citable) accession number: P0AGL5
Secondary accession number(s): P52121
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.