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P0AGL5 (RATA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosome association toxin RatA
Alternative name(s):
Toxin RatA
Gene names
Name:ratA
Synonyms:pasT, yfjG
Ordered Locus Names:b2619, JW2600
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length158 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Toxic component of a toxin-antitoxin (TA) module. Binds to 50S ribosomal subunits, preventing them from associating with 30S subunits to form 70S ribosomes and reducing polysomes. It does not cause ribosomes to dissociate however. The antibiotic paromomycin blocks the anti-association activity of RatA. Overexpression results in inhibition of growth in liquid cultures, and in a decrease in protein translation. The other gene of this operon, ratB, is not the cognate antitoxin in this strain; in CFT073 however it does fulfill this function. Ref.5 Ref.6 Ref.7

Low level expression in a deletion mutant increases resistance to acidified sodium nitrate which causes nitrosative stress. Ref.5 Ref.6 Ref.7

Subunit structure

Associates with 50S ribosomes. Ref.6

Disruption phenotype

Deletion of the ratA-ratB operon has no effect on bacterial persistence on rich medium for this strain, but does affect bacterial persistence for E.coli strain CFT073. Ref.7

Sequence similarities

Belongs to the ribosome association toxin RatA family.

Ontologies

Keywords
   Biological processStress response
   Molecular functionToxin
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processnegative regulation of translational initiation

Inferred from mutant phenotype Ref.6. Source: EcoCyc

response to stress

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionribosomal large subunit binding

Inferred from direct assay Ref.6. Source: EcoCyc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 158158Ribosome association toxin RatA
PRO_0000192474

Sequences

Sequence LengthMass (Da)Tools
P0AGL5 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: 68A3A8FBE6AC83D5

FASTA15817,727
        10         20         30         40         50         60 
MILFVGFLLM EIVMPQISRT ALVPYSAEQM YQLVNDVQSY PQFLPGCTGS RILESTPGQM 

        70         80         90        100        110        120 
TAAVDVSKAG ISKTFTTRNQ LTSNQSILMN LVDGPFKKLI GGWKFTPLSQ EACRIEFHLD 

       130        140        150 
FEFTNKLIEL AFGRVFKELA ANMVQAFTVR AKEVYSAR 

« Hide

References

« Hide 'large scale' references
[1]"A tRNA-like structure is present in 10Sa RNA, a small stable RNA from Escherichia coli."
Komine Y., Kitabatake M., Yokogawa T., Nishikawa K., Inokuchi H.
Proc. Natl. Acad. Sci. U.S.A. 91:9223-9227(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. expand/collapse author list , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"A novel family of Escherichia coli toxin-antitoxin gene pairs."
Brown J.M., Shaw K.J.
J. Bacteriol. 185:6600-6608(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A TOXIN.
Strain: K12 / MG1655 / ATCC 47076.
[6]"RatA (YfjG), an Escherichia coli toxin, inhibits 70S ribosome association to block translation initiation."
Zhang Y., Inouye M.
Mol. Microbiol. 79:1418-1429(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A TOXIN, SUBUNIT.
[7]"Toxin-antitoxin systems are important for niche-specific colonization and stress resistance of uropathogenic Escherichia coli."
Norton J.P., Mulvey M.A.
PLoS Pathog. 8:E1002954-E1002954(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ROLE IN STRESS RESPONSE, DISRUPTION PHENOTYPE.
Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D12501 Genomic DNA. No translation available.
U36840 Genomic DNA. Translation: AAA79789.1.
U00096 Genomic DNA. Translation: AAC75668.1.
AP009048 Genomic DNA. Translation: BAA16504.1.
PIRT08632.
RefSeqNP_417109.1. NC_000913.3.
YP_490841.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0AGL5.
SMRP0AGL5. Positions 20-157.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP0AGL5. 1 interaction.
STRING511145.b2619.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75668; AAC75668; b2619.
BAA16504; BAA16504; BAA16504.
GeneID12934447.
945614.
KEGGecj:Y75_p2566.
eco:b2619.
PATRIC32120637. VBIEscCol129921_2717.

Organism-specific databases

EchoBASEEB2985.
EcoGeneEG13193. ratA.

Phylogenomic databases

eggNOGCOG2867.
HOGENOMHOG000261730.
OMADGPFKYL.
OrthoDBEOG6RFZZ5.
PhylomeDBP0AGL5.
ProtClustDBPRK10724.

Enzyme and pathway databases

BioCycEcoCyc:G7358-MONOMER.
ECOL316407:JW2600-MONOMER.

Gene expression databases

GenevestigatorP0AGL5.

Family and domain databases

Gene3D3.30.530.20. 1 hit.
InterProIPR005031. Polyket_cyc.
IPR023393. START-like_dom.
[Graphical view]
PfamPF03364. Polyketide_cyc. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP0AGL5.

Entry information

Entry nameRATA_ECOLI
AccessionPrimary (citable) accession number: P0AGL5
Secondary accession number(s): P52121
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: April 16, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Uncharacterized protein families (UPF)

List of uncharacterized protein family (UPF) entries

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene