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Protein

Ribosome association toxin RatA

Gene

ratA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Toxic component of a type II toxin-antitoxin (TA) system. Binds to 50S ribosomal subunits, preventing them from associating with 30S subunits to form 70S ribosomes and reducing polysomes. It does not cause ribosomes to dissociate however. The antibiotic paromomycin blocks the anti-association activity of RatA. Overexpression results in inhibition of growth in liquid cultures, and in a decrease in protein translation. The other gene of this operon, ratB, is not the cognate antitoxin in this strain; in CFT073 however it does fulfill this function.
Low level expression in a deletion mutant increases resistance to acidified sodium nitrate which causes nitrosative stress.

GO - Molecular functioni

  • ribosomal large subunit binding Source: EcoCyc

GO - Biological processi

  • negative regulation of translational initiation Source: EcoCyc

Keywordsi

Biological processStress response, Toxin-antitoxin system

Enzyme and pathway databases

BioCyciEcoCyc:G7358-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome association toxin RatA
Alternative name(s):
Toxin RatA
Gene namesi
Name:ratA
Synonyms:pasT, yfjG
Ordered Locus Names:b2619, JW2600
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13193 ratA

Pathology & Biotechi

Disruption phenotypei

Deletion of the ratA-ratB operon has no effect on bacterial persistence on rich medium for this strain, but does affect bacterial persistence for E.coli strain CFT073.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001924741 – 158Ribosome association toxin RatAAdd BLAST158

Proteomic databases

PaxDbiP0AGL5
PRIDEiP0AGL5

Interactioni

Subunit structurei

Associates with 50S ribosomes.1 Publication

Protein-protein interaction databases

BioGridi4263441, 17 interactors
IntActiP0AGL5, 1 interactor
STRINGi316385.ECDH10B_2785

Structurei

3D structure databases

ProteinModelPortaliP0AGL5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108Z1B Bacteria
COG2867 LUCA
HOGENOMiHOG000261730
InParanoidiP0AGL5
OMAiYIDGPFK
PhylomeDBiP0AGL5

Family and domain databases

Gene3Di3.30.530.20, 1 hit
InterProiView protein in InterPro
IPR005031 COQ10_START
IPR023393 START-like_dom_sf
PfamiView protein in Pfam
PF03364 Polyketide_cyc, 1 hit

Sequencei

Sequence statusi: Complete.

P0AGL5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILFVGFLLM EIVMPQISRT ALVPYSAEQM YQLVNDVQSY PQFLPGCTGS
60 70 80 90 100
RILESTPGQM TAAVDVSKAG ISKTFTTRNQ LTSNQSILMN LVDGPFKKLI
110 120 130 140 150
GGWKFTPLSQ EACRIEFHLD FEFTNKLIEL AFGRVFKELA ANMVQAFTVR

AKEVYSAR
Length:158
Mass (Da):17,727
Last modified:December 20, 2005 - v1
Checksum:i68A3A8FBE6AC83D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12501 Genomic DNA No translation available.
U36840 Genomic DNA Translation: AAA79789.1
U00096 Genomic DNA Translation: AAC75668.1
AP009048 Genomic DNA Translation: BAA16504.1
PIRiT08632
RefSeqiNP_417109.1, NC_000913.3
WP_000600189.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75668; AAC75668; b2619
BAA16504; BAA16504; BAA16504
GeneIDi945614
KEGGiecj:JW2600
eco:b2619
PATRICifig|511145.12.peg.2717

Similar proteinsi

Entry informationi

Entry nameiRATA_ECOLI
AccessioniPrimary (citable) accession number: P0AGL5
Secondary accession number(s): P52121
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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