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P0AGL5

- RATA_ECOLI

UniProt

P0AGL5 - RATA_ECOLI

Protein

Ribosome association toxin RatA

Gene

ratA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (20 Dec 2005)
      Previous versions | rss
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    Functioni

    Toxic component of a toxin-antitoxin (TA) module. Binds to 50S ribosomal subunits, preventing them from associating with 30S subunits to form 70S ribosomes and reducing polysomes. It does not cause ribosomes to dissociate however. The antibiotic paromomycin blocks the anti-association activity of RatA. Overexpression results in inhibition of growth in liquid cultures, and in a decrease in protein translation. The other gene of this operon, ratB, is not the cognate antitoxin in this strain; in CFT073 however it does fulfill this function.
    Low level expression in a deletion mutant increases resistance to acidified sodium nitrate which causes nitrosative stress.

    GO - Molecular functioni

    1. ribosomal large subunit binding Source: EcoCyc

    GO - Biological processi

    1. negative regulation of translational initiation Source: EcoCyc
    2. response to stress Source: UniProtKB-KW

    Keywords - Molecular functioni

    Toxin

    Keywords - Biological processi

    Stress response

    Enzyme and pathway databases

    BioCyciEcoCyc:G7358-MONOMER.
    ECOL316407:JW2600-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribosome association toxin RatA
    Alternative name(s):
    Toxin RatA
    Gene namesi
    Name:ratA
    Synonyms:pasT, yfjG
    Ordered Locus Names:b2619, JW2600
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13193. ratA.

    Pathology & Biotechi

    Disruption phenotypei

    Deletion of the ratA-ratB operon has no effect on bacterial persistence on rich medium for this strain, but does affect bacterial persistence for E.coli strain CFT073.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 158158Ribosome association toxin RatAPRO_0000192474Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP0AGL5.

    Interactioni

    Subunit structurei

    Associates with 50S ribosomes.1 Publication

    Protein-protein interaction databases

    IntActiP0AGL5. 1 interaction.
    STRINGi511145.b2619.

    Structurei

    3D structure databases

    ProteinModelPortaliP0AGL5.
    SMRiP0AGL5. Positions 20-157.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2867.
    HOGENOMiHOG000261730.
    OMAiFNQAANT.
    OrthoDBiEOG6RFZZ5.
    PhylomeDBiP0AGL5.

    Family and domain databases

    Gene3Di3.30.530.20. 1 hit.
    InterProiIPR005031. Polyket_cyc.
    IPR023393. START-like_dom.
    [Graphical view]
    PfamiPF03364. Polyketide_cyc. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0AGL5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MILFVGFLLM EIVMPQISRT ALVPYSAEQM YQLVNDVQSY PQFLPGCTGS    50
    RILESTPGQM TAAVDVSKAG ISKTFTTRNQ LTSNQSILMN LVDGPFKKLI 100
    GGWKFTPLSQ EACRIEFHLD FEFTNKLIEL AFGRVFKELA ANMVQAFTVR 150
    AKEVYSAR 158
    Length:158
    Mass (Da):17,727
    Last modified:December 20, 2005 - v1
    Checksum:i68A3A8FBE6AC83D5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D12501 Genomic DNA. No translation available.
    U36840 Genomic DNA. Translation: AAA79789.1.
    U00096 Genomic DNA. Translation: AAC75668.1.
    AP009048 Genomic DNA. Translation: BAA16504.1.
    PIRiT08632.
    RefSeqiNP_417109.1. NC_000913.3.
    YP_490841.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75668; AAC75668; b2619.
    BAA16504; BAA16504; BAA16504.
    GeneIDi12934447.
    945614.
    KEGGiecj:Y75_p2566.
    eco:b2619.
    PATRICi32120637. VBIEscCol129921_2717.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D12501 Genomic DNA. No translation available.
    U36840 Genomic DNA. Translation: AAA79789.1 .
    U00096 Genomic DNA. Translation: AAC75668.1 .
    AP009048 Genomic DNA. Translation: BAA16504.1 .
    PIRi T08632.
    RefSeqi NP_417109.1. NC_000913.3.
    YP_490841.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0AGL5.
    SMRi P0AGL5. Positions 20-157.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P0AGL5. 1 interaction.
    STRINGi 511145.b2619.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75668 ; AAC75668 ; b2619 .
    BAA16504 ; BAA16504 ; BAA16504 .
    GeneIDi 12934447.
    945614.
    KEGGi ecj:Y75_p2566.
    eco:b2619.
    PATRICi 32120637. VBIEscCol129921_2717.

    Organism-specific databases

    EchoBASEi EB2985.
    EcoGenei EG13193. ratA.

    Phylogenomic databases

    eggNOGi COG2867.
    HOGENOMi HOG000261730.
    OMAi FNQAANT.
    OrthoDBi EOG6RFZZ5.
    PhylomeDBi P0AGL5.

    Enzyme and pathway databases

    BioCyci EcoCyc:G7358-MONOMER.
    ECOL316407:JW2600-MONOMER.

    Miscellaneous databases

    PROi P0AGL5.

    Gene expression databases

    Genevestigatori P0AGL5.

    Family and domain databases

    Gene3Di 3.30.530.20. 1 hit.
    InterProi IPR005031. Polyket_cyc.
    IPR023393. START-like_dom.
    [Graphical view ]
    Pfami PF03364. Polyketide_cyc. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A tRNA-like structure is present in 10Sa RNA, a small stable RNA from Escherichia coli."
      Komine Y., Kitabatake M., Yokogawa T., Nishikawa K., Inokuchi H.
      Proc. Natl. Acad. Sci. U.S.A. 91:9223-9227(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
      Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
      , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
      DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "A novel family of Escherichia coli toxin-antitoxin gene pairs."
      Brown J.M., Shaw K.J.
      J. Bacteriol. 185:6600-6608(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A TOXIN.
      Strain: K12 / MG1655 / ATCC 47076.
    6. "RatA (YfjG), an Escherichia coli toxin, inhibits 70S ribosome association to block translation initiation."
      Zhang Y., Inouye M.
      Mol. Microbiol. 79:1418-1429(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A TOXIN, SUBUNIT.
    7. "Toxin-antitoxin systems are important for niche-specific colonization and stress resistance of uropathogenic Escherichia coli."
      Norton J.P., Mulvey M.A.
      PLoS Pathog. 8:E1002954-E1002954(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ROLE IN STRESS RESPONSE, DISRUPTION PHENOTYPE.
      Strain: K12 / MG1655 / ATCC 47076.

    Entry informationi

    Entry nameiRATA_ECOLI
    AccessioniPrimary (citable) accession number: P0AGL5
    Secondary accession number(s): P52121
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families
    3. Uncharacterized protein families (UPF)
      List of uncharacterized protein family (UPF) entries

    External Data

    Dasty 3