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P0AGL5

- RATA_ECOLI

UniProt

P0AGL5 - RATA_ECOLI

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Protein

Ribosome association toxin RatA

Gene

ratA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Toxic component of a toxin-antitoxin (TA) module. Binds to 50S ribosomal subunits, preventing them from associating with 30S subunits to form 70S ribosomes and reducing polysomes. It does not cause ribosomes to dissociate however. The antibiotic paromomycin blocks the anti-association activity of RatA. Overexpression results in inhibition of growth in liquid cultures, and in a decrease in protein translation. The other gene of this operon, ratB, is not the cognate antitoxin in this strain; in CFT073 however it does fulfill this function.
Low level expression in a deletion mutant increases resistance to acidified sodium nitrate which causes nitrosative stress.

GO - Molecular functioni

  1. ribosomal large subunit binding Source: EcoCyc

GO - Biological processi

  1. negative regulation of translational initiation Source: EcoCyc
  2. response to stress Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Toxin

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciEcoCyc:G7358-MONOMER.
ECOL316407:JW2600-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome association toxin RatA
Alternative name(s):
Toxin RatA
Gene namesi
Name:ratA
Synonyms:pasT, yfjG
Ordered Locus Names:b2619, JW2600
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13193. ratA.

Pathology & Biotechi

Disruption phenotypei

Deletion of the ratA-ratB operon has no effect on bacterial persistence on rich medium for this strain, but does affect bacterial persistence for E.coli strain CFT073.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 158158Ribosome association toxin RatAPRO_0000192474Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP0AGL5.

Interactioni

Subunit structurei

Associates with 50S ribosomes.1 Publication

Protein-protein interaction databases

IntActiP0AGL5. 1 interaction.
STRINGi511145.b2619.

Structurei

3D structure databases

ProteinModelPortaliP0AGL5.
SMRiP0AGL5. Positions 20-157.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2867.
HOGENOMiHOG000261730.
InParanoidiP0AGL5.
OMAiFNQAANT.
OrthoDBiEOG6RFZZ5.
PhylomeDBiP0AGL5.

Family and domain databases

Gene3Di3.30.530.20. 1 hit.
InterProiIPR005031. Polyket_cyc.
IPR023393. START-like_dom.
[Graphical view]
PfamiPF03364. Polyketide_cyc. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AGL5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MILFVGFLLM EIVMPQISRT ALVPYSAEQM YQLVNDVQSY PQFLPGCTGS
60 70 80 90 100
RILESTPGQM TAAVDVSKAG ISKTFTTRNQ LTSNQSILMN LVDGPFKKLI
110 120 130 140 150
GGWKFTPLSQ EACRIEFHLD FEFTNKLIEL AFGRVFKELA ANMVQAFTVR

AKEVYSAR
Length:158
Mass (Da):17,727
Last modified:December 20, 2005 - v1
Checksum:i68A3A8FBE6AC83D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12501 Genomic DNA. No translation available.
U36840 Genomic DNA. Translation: AAA79789.1.
U00096 Genomic DNA. Translation: AAC75668.1.
AP009048 Genomic DNA. Translation: BAA16504.1.
PIRiT08632.
RefSeqiNP_417109.1. NC_000913.3.
YP_490841.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75668; AAC75668; b2619.
BAA16504; BAA16504; BAA16504.
GeneIDi12934447.
945614.
KEGGiecj:Y75_p2566.
eco:b2619.
PATRICi32120637. VBIEscCol129921_2717.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12501 Genomic DNA. No translation available.
U36840 Genomic DNA. Translation: AAA79789.1 .
U00096 Genomic DNA. Translation: AAC75668.1 .
AP009048 Genomic DNA. Translation: BAA16504.1 .
PIRi T08632.
RefSeqi NP_417109.1. NC_000913.3.
YP_490841.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0AGL5.
SMRi P0AGL5. Positions 20-157.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P0AGL5. 1 interaction.
STRINGi 511145.b2619.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75668 ; AAC75668 ; b2619 .
BAA16504 ; BAA16504 ; BAA16504 .
GeneIDi 12934447.
945614.
KEGGi ecj:Y75_p2566.
eco:b2619.
PATRICi 32120637. VBIEscCol129921_2717.

Organism-specific databases

EchoBASEi EB2985.
EcoGenei EG13193. ratA.

Phylogenomic databases

eggNOGi COG2867.
HOGENOMi HOG000261730.
InParanoidi P0AGL5.
OMAi FNQAANT.
OrthoDBi EOG6RFZZ5.
PhylomeDBi P0AGL5.

Enzyme and pathway databases

BioCyci EcoCyc:G7358-MONOMER.
ECOL316407:JW2600-MONOMER.

Miscellaneous databases

PROi P0AGL5.

Gene expression databases

Genevestigatori P0AGL5.

Family and domain databases

Gene3Di 3.30.530.20. 1 hit.
InterProi IPR005031. Polyket_cyc.
IPR023393. START-like_dom.
[Graphical view ]
Pfami PF03364. Polyketide_cyc. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A tRNA-like structure is present in 10Sa RNA, a small stable RNA from Escherichia coli."
    Komine Y., Kitabatake M., Yokogawa T., Nishikawa K., Inokuchi H.
    Proc. Natl. Acad. Sci. U.S.A. 91:9223-9227(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "A novel family of Escherichia coli toxin-antitoxin gene pairs."
    Brown J.M., Shaw K.J.
    J. Bacteriol. 185:6600-6608(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A TOXIN.
    Strain: K12 / MG1655 / ATCC 47076.
  6. "RatA (YfjG), an Escherichia coli toxin, inhibits 70S ribosome association to block translation initiation."
    Zhang Y., Inouye M.
    Mol. Microbiol. 79:1418-1429(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A TOXIN, SUBUNIT.
  7. "Toxin-antitoxin systems are important for niche-specific colonization and stress resistance of uropathogenic Escherichia coli."
    Norton J.P., Mulvey M.A.
    PLoS Pathog. 8:E1002954-E1002954(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN STRESS RESPONSE, DISRUPTION PHENOTYPE.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiRATA_ECOLI
AccessioniPrimary (citable) accession number: P0AGL5
Secondary accession number(s): P52121
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: October 29, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

External Data

Dasty 3