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Protein

HTH-type transcriptional regulator IscR

Gene

iscR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Regulates the transcription of several operons and genes involved in the biogenesis of Fe-S clusters and Fe-S-containing proteins. Transcriptional repressor of the iscRSUA operon, which is involved in the assembly of Fe-S clusters into Fe-S proteins. In its apoform, under conditions of oxidative stress or iron deprivation, it activates the suf operon, which is a second operon involved in the assembly of Fe-S clusters. Represses its own transcription as well as that of toxin rnlA.3 Publications

Cofactori

[2Fe-2S] cluster2 PublicationsNote: Binds 1 [2Fe-2S] cluster; whether or not the Fe-S cluster is necessary for transcriptional regulation depends on the type of IscR-binding sequence.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi92Iron-sulfur (2Fe-2S)Sequence analysis1
Metal bindingi98Iron-sulfur (2Fe-2S)Sequence analysis1
Metal bindingi104Iron-sulfur (2Fe-2S)Sequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi28 – 51H-T-H motifBy similarityAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Stress response, Transcription, Transcription regulation

Keywords - Ligandi

2Fe-2S, DNA-binding, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7326-MONOMER.
ECOL316407:JW2515-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator IscR
Gene namesi
Name:iscR
Synonyms:yfhP
Ordered Locus Names:b2531, JW2515
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13397. iscR.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi92C → A: No effect on activation of the suf operon, no effect on repression of rnlA. 2 Publications1
Mutagenesisi98C → A: No effect on activation of the suf operon, no effect on repression of rnlA. 2 Publications1
Mutagenesisi104C → A: No effect on activation of the suf operon, no effect on repression of rnlA. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001095601 – 162HTH-type transcriptional regulator IscRAdd BLAST162

Proteomic databases

EPDiP0AGK8.
PaxDbiP0AGK8.
PRIDEiP0AGK8.

Expressioni

Inductioni

By oxidative stress conditions and iron starvation.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4259433. 59 interactors.
DIPiDIP-47974N.
IntActiP0AGK8. 3 interactors.
STRINGi511145.b2531.

Structurei

Secondary structure

1162
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 20Combined sources16
Helixi21 – 23Combined sources3
Helixi28 – 35Combined sources8
Helixi39 – 51Combined sources13
Beta strandi54 – 58Combined sources5
Beta strandi60 – 62Combined sources3
Beta strandi64 – 69Combined sources6
Helixi71 – 73Combined sources3
Helixi76 – 83Combined sources8
Helixi97 – 99Combined sources3
Helixi101 – 121Combined sources21
Helixi126 – 132Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HF0X-ray1.90A/B1-133[»]
4HF1X-ray2.22A/B1-162[»]
4HF2X-ray2.99A/B1-162[»]
ProteinModelPortaliP0AGK8.
SMRiP0AGK8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 131HTH rrf2-typeAdd BLAST130

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108ZF1. Bacteria.
COG1959. LUCA.
HOGENOMiHOG000249812.
InParanoidiP0AGK8.
KOiK13643.
OMAiMTHDLWS.
PhylomeDBiP0AGK8.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_01176. HTH_type_IscR. 1 hit.
InterProiIPR010242. TF_HTH_IscR.
IPR030489. TR_Rrf2-type_CS.
IPR000944. Tscrpt_reg_Rrf2-type.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF02082. Rrf2. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
TIGRFAMsiTIGR02010. IscR. 1 hit.
TIGR00738. rrf2_super. 1 hit.
PROSITEiPS01332. HTH_RRF2_1. 1 hit.
PS51197. HTH_RRF2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AGK8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLTSKGRYA VTAMLDVALN SEAGPVPLAD ISERQGISLS YLEQLFSRLR
60 70 80 90 100
KNGLVSSVRG PGGGYLLGKD ASSIAVGEVI SAVDESVDAT RCQGKGGCQG
110 120 130 140 150
GDKCLTHALW RDLSDRLTGF LNNITLGELV NNQEVLDVSG RQHTHDAPRT
160
RTQDAIDVKL RA
Length:162
Mass (Da):17,337
Last modified:December 20, 2005 - v1
Checksum:iDE82977C3774E3BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75584.1.
AP009048 Genomic DNA. Translation: BAA16425.1.
PIRiB65030.
RefSeqiNP_417026.1. NC_000913.3.
WP_001241357.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75584; AAC75584; b2531.
BAA16425; BAA16425; BAA16425.
GeneIDi945279.
KEGGiecj:JW2515.
eco:b2531.
PATRICi32120459. VBIEscCol129921_2632.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75584.1.
AP009048 Genomic DNA. Translation: BAA16425.1.
PIRiB65030.
RefSeqiNP_417026.1. NC_000913.3.
WP_001241357.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HF0X-ray1.90A/B1-133[»]
4HF1X-ray2.22A/B1-162[»]
4HF2X-ray2.99A/B1-162[»]
ProteinModelPortaliP0AGK8.
SMRiP0AGK8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259433. 59 interactors.
DIPiDIP-47974N.
IntActiP0AGK8. 3 interactors.
STRINGi511145.b2531.

Proteomic databases

EPDiP0AGK8.
PaxDbiP0AGK8.
PRIDEiP0AGK8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75584; AAC75584; b2531.
BAA16425; BAA16425; BAA16425.
GeneIDi945279.
KEGGiecj:JW2515.
eco:b2531.
PATRICi32120459. VBIEscCol129921_2632.

Organism-specific databases

EchoBASEiEB3178.
EcoGeneiEG13397. iscR.

Phylogenomic databases

eggNOGiENOG4108ZF1. Bacteria.
COG1959. LUCA.
HOGENOMiHOG000249812.
InParanoidiP0AGK8.
KOiK13643.
OMAiMTHDLWS.
PhylomeDBiP0AGK8.

Enzyme and pathway databases

BioCyciEcoCyc:G7326-MONOMER.
ECOL316407:JW2515-MONOMER.

Miscellaneous databases

PROiP0AGK8.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_01176. HTH_type_IscR. 1 hit.
InterProiIPR010242. TF_HTH_IscR.
IPR030489. TR_Rrf2-type_CS.
IPR000944. Tscrpt_reg_Rrf2-type.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF02082. Rrf2. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
TIGRFAMsiTIGR02010. IscR. 1 hit.
TIGR00738. rrf2_super. 1 hit.
PROSITEiPS01332. HTH_RRF2_1. 1 hit.
PS51197. HTH_RRF2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiISCR_ECOLI
AccessioniPrimary (citable) accession number: P0AGK8
Secondary accession number(s): P77484
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.