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Protein

HTH-type transcriptional regulator IscR

Gene

iscR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulates the transcription of several operons and genes involved in the biogenesis of Fe-S clusters and Fe-S-containing proteins. Transcriptional repressor of the iscRSUA operon, which is involved in the assembly of Fe-S clusters into Fe-S proteins. In its apoform, under conditions of oxidative stress or iron deprivation, it activates the suf operon, which is a second operon involved in the assembly of Fe-S clusters. Represses its own transcription as well as that of toxin rnlA.3 Publications

Cofactori

[2Fe-2S] cluster2 PublicationsNote: Binds 1 [2Fe-2S] cluster; whether or not the Fe-S cluster is necessary for transcriptional regulation depends on the type of IscR-binding sequence.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi92Iron-sulfur (2Fe-2S)Sequence analysis1
Metal bindingi98Iron-sulfur (2Fe-2S)Sequence analysis1
Metal bindingi104Iron-sulfur (2Fe-2S)Sequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi28 – 51H-T-H motifBy similarityAdd BLAST24

GO - Molecular functioni

  • 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  • DNA binding transcription factor activity Source: EcoCyc
  • double-stranded DNA binding Source: InterPro
  • identical protein binding Source: IntAct
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processStress response, Transcription, Transcription regulation
Ligand2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7326-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator IscR
Gene namesi
Name:iscR
Synonyms:yfhP
Ordered Locus Names:b2531, JW2515
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13397 iscR

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi92C → A: No effect on activation of the suf operon, no effect on repression of rnlA. 2 Publications1
Mutagenesisi98C → A: No effect on activation of the suf operon, no effect on repression of rnlA. 2 Publications1
Mutagenesisi104C → A: No effect on activation of the suf operon, no effect on repression of rnlA. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001095601 – 162HTH-type transcriptional regulator IscRAdd BLAST162

Proteomic databases

EPDiP0AGK8
PaxDbiP0AGK8
PRIDEiP0AGK8

Expressioni

Inductioni

By oxidative stress conditions and iron starvation.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-1129278,EBI-1129278

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi425943382 interactors.
DIPiDIP-47974N
IntActiP0AGK8 4 interactors.
STRINGi316385.ECDH10B_2698

Structurei

Secondary structure

1162
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 20Combined sources16
Helixi21 – 23Combined sources3
Helixi28 – 35Combined sources8
Helixi39 – 51Combined sources13
Beta strandi54 – 58Combined sources5
Beta strandi60 – 62Combined sources3
Beta strandi64 – 69Combined sources6
Helixi71 – 73Combined sources3
Helixi76 – 83Combined sources8
Helixi97 – 99Combined sources3
Helixi101 – 121Combined sources21
Helixi126 – 132Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HF0X-ray1.90A/B1-133[»]
4HF1X-ray2.22A/B1-162[»]
4HF2X-ray2.99A/B1-162[»]
ProteinModelPortaliP0AGK8
SMRiP0AGK8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 131HTH rrf2-typeAdd BLAST130

Phylogenomic databases

eggNOGiENOG4108ZF1 Bacteria
COG1959 LUCA
HOGENOMiHOG000249812
InParanoidiP0AGK8
KOiK13643
OMAiLTTRGHY
PhylomeDBiP0AGK8

Family and domain databases

Gene3Di1.10.10.101 hit
HAMAPiMF_01176 HTH_type_IscR, 1 hit
InterProiView protein in InterPro
IPR010242 TF_HTH_IscR
IPR030489 TR_Rrf2-type_CS
IPR000944 Tscrpt_reg_Rrf2-type
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR33221 PTHR33221, 1 hit
PfamiView protein in Pfam
PF02082 Rrf2, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
TIGRFAMsiTIGR02010 IscR, 1 hit
TIGR00738 rrf2_super, 1 hit
PROSITEiView protein in PROSITE
PS01332 HTH_RRF2_1, 1 hit
PS51197 HTH_RRF2_2, 1 hit

Sequencei

Sequence statusi: Complete.

P0AGK8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLTSKGRYA VTAMLDVALN SEAGPVPLAD ISERQGISLS YLEQLFSRLR
60 70 80 90 100
KNGLVSSVRG PGGGYLLGKD ASSIAVGEVI SAVDESVDAT RCQGKGGCQG
110 120 130 140 150
GDKCLTHALW RDLSDRLTGF LNNITLGELV NNQEVLDVSG RQHTHDAPRT
160
RTQDAIDVKL RA
Length:162
Mass (Da):17,337
Last modified:December 20, 2005 - v1
Checksum:iDE82977C3774E3BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75584.1
AP009048 Genomic DNA Translation: BAA16425.1
PIRiB65030
RefSeqiNP_417026.1, NC_000913.3
WP_001241357.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75584; AAC75584; b2531
BAA16425; BAA16425; BAA16425
GeneIDi945279
KEGGiecj:JW2515
eco:b2531
PATRICifig|1411691.4.peg.4203

Similar proteinsi

Entry informationi

Entry nameiISCR_ECOLI
AccessioniPrimary (citable) accession number: P0AGK8
Secondary accession number(s): P77484
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome