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Protein

4-hydroxybenzoate octaprenyltransferase

Gene

ubiA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the prenylation of para-hydroxybenzoate (PHB) with an all-trans polyprenyl group. Mediates the second step in the final reaction sequence of ubiquinone-8 (UQ-8) biosynthesis, which is the condensation of the polyisoprenoid side chain with PHB, generating the first membrane-bound Q intermediate 3-octaprenyl-4-hydroxybenzoate.UniRule annotation2 Publications

Catalytic activityi

4-hydroxybenzoate + farnesylfarnesylgeraniol = 3-octaprenyl-4-hydroxybenzoate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: ubiquinone biosynthesis

This protein is involved in the pathway ubiquinone biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway ubiquinone biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

  • 4-hydroxybenzoate octaprenyltransferase activity Source: EcoliWiki
  • transferase activity, transferring alkyl or aryl (other than methyl) groups Source: EcoCyc

GO - Biological processi

  • ubiquinone biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Ubiquinone biosynthesis

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciEcoCyc:4OHBENZOATE-OCTAPRENYLTRANSFER-MONOMER.
ECOL316407:JW4000-MONOMER.
MetaCyc:4OHBENZOATE-OCTAPRENYLTRANSFER-MONOMER.
UniPathwayiUPA00232.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxybenzoate octaprenyltransferaseUniRule annotation (EC:2.5.1.-UniRule annotation)
Alternative name(s):
4-HB polyprenyltransferaseUniRule annotation
Gene namesi
Name:ubiAUniRule annotation
Synonyms:cyr
Ordered Locus Names:b4040, JW4000
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11370. ubiA.

Subcellular locationi

  • Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2222PeriplasmicSequence analysisAdd
BLAST
Transmembranei23 – 4321HelicalUniRule annotationAdd
BLAST
Topological domaini44 – 452Cytoplasmic1 Publication
Transmembranei46 – 6621HelicalUniRule annotationAdd
BLAST
Topological domaini67 – 9933Periplasmic1 PublicationAdd
BLAST
Transmembranei100 – 11920HelicalUniRule annotationAdd
BLAST
Topological domaini120 – 14021Cytoplasmic1 PublicationAdd
BLAST
Transmembranei141 – 16121HelicalUniRule annotationAdd
BLAST
Topological domaini162 – 1621Periplasmic1 Publication
Transmembranei163 – 18321HelicalUniRule annotationAdd
BLAST
Topological domaini184 – 21229Cytoplasmic1 PublicationAdd
BLAST
Transmembranei213 – 23321HelicalUniRule annotationAdd
BLAST
Transmembranei234 – 25421HelicalUniRule annotationAdd
BLAST
Topological domaini255 – 26713Cytoplasmic1 PublicationAdd
BLAST
Transmembranei268 – 28821HelicalUniRule annotationAdd
BLAST
Topological domaini289 – 2902PeriplasmicSequence analysis

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2902904-hydroxybenzoate octaprenyltransferasePRO_0000162890Add
BLAST

Proteomic databases

PaxDbiP0AGK1.

Interactioni

Protein-protein interaction databases

BioGridi4259714. 333 interactions.
STRINGi511145.b4040.

Structurei

3D structure databases

ProteinModelPortaliP0AGK1.
SMRiP0AGK1. Positions 51-208.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UbiA prenyltransferase family.UniRule annotationCurated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C4G. Bacteria.
COG0382. LUCA.
HOGENOMiHOG000003696.
InParanoidiP0AGK1.
KOiK03179.
OMAiIPMAFMA.
PhylomeDBiP0AGK1.

Family and domain databases

HAMAPiMF_01635. UbiA. 1 hit.
InterProiIPR031103. HB_octoprenylTrfase.
IPR006370. HB_polyprenyltransferase.
IPR000537. UbiA_prenyltransferase.
IPR030470. UbiA_prenylTrfase_CS.
[Graphical view]
PfamiPF01040. UbiA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01474. ubiA_proteo. 1 hit.
PROSITEiPS00943. UBIA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AGK1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEWSLTQNKL LAFHRLMRTD KPIGALLLLW PTLWALWVAT PGVPQLWILA
60 70 80 90 100
VFVAGVWLMR AAGCVVNDYA DRKFDGHVKR TANRPLPSGA VTEKEARALF
110 120 130 140 150
VVLVLISFLL VLTLNTMTIL LSIAALALAW VYPFMKRYTH LPQVVLGAAF
160 170 180 190 200
GWSIPMAFAA VSESVPLSCW LMFLANILWA VAYDTQYAMV DRDDDVKIGI
210 220 230 240 250
KSTAILFGQY DKLIIGILQI GVLALMAIIG ELNGLGWGYY WSILVAGALF
260 270 280 290
VYQQKLIANR EREACFKAFM NNNYVGLVLF LGLAMSYWHF
Length:290
Mass (Da):32,512
Last modified:December 20, 2005 - v1
Checksum:iF10FED1D7A30E115
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93136 Genomic DNA. Translation: AAA24712.1.
M93413 Genomic DNA. Translation: AAA24717.1.
X66619 Genomic DNA. Translation: CAA47182.1.
X57434 Genomic DNA. Translation: CAA40682.1.
X69522 Genomic DNA. Translation: CAA49270.1.
DQ087228 Genomic DNA. Translation: AAY88960.1.
U00006 Genomic DNA. Translation: AAC43134.1.
U00096 Genomic DNA. Translation: AAC77010.1.
AP009048 Genomic DNA. Translation: BAE78042.1.
M96268 Unassigned DNA. Translation: AAA17028.1.
X63407 Genomic DNA. Translation: CAA45003.1.
PIRiJC2316.
RefSeqiNP_418464.1. NC_000913.3.
WP_000455227.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77010; AAC77010; b4040.
BAE78042; BAE78042; BAE78042.
GeneIDi948540.
KEGGiecj:JW4000.
eco:b4040.
PATRICi32123617. VBIEscCol129921_4157.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93136 Genomic DNA. Translation: AAA24712.1.
M93413 Genomic DNA. Translation: AAA24717.1.
X66619 Genomic DNA. Translation: CAA47182.1.
X57434 Genomic DNA. Translation: CAA40682.1.
X69522 Genomic DNA. Translation: CAA49270.1.
DQ087228 Genomic DNA. Translation: AAY88960.1.
U00006 Genomic DNA. Translation: AAC43134.1.
U00096 Genomic DNA. Translation: AAC77010.1.
AP009048 Genomic DNA. Translation: BAE78042.1.
M96268 Unassigned DNA. Translation: AAA17028.1.
X63407 Genomic DNA. Translation: CAA45003.1.
PIRiJC2316.
RefSeqiNP_418464.1. NC_000913.3.
WP_000455227.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AGK1.
SMRiP0AGK1. Positions 51-208.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259714. 333 interactions.
STRINGi511145.b4040.

Proteomic databases

PaxDbiP0AGK1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77010; AAC77010; b4040.
BAE78042; BAE78042; BAE78042.
GeneIDi948540.
KEGGiecj:JW4000.
eco:b4040.
PATRICi32123617. VBIEscCol129921_4157.

Organism-specific databases

EchoBASEiEB1344.
EcoGeneiEG11370. ubiA.

Phylogenomic databases

eggNOGiENOG4105C4G. Bacteria.
COG0382. LUCA.
HOGENOMiHOG000003696.
InParanoidiP0AGK1.
KOiK03179.
OMAiIPMAFMA.
PhylomeDBiP0AGK1.

Enzyme and pathway databases

UniPathwayiUPA00232.
BioCyciEcoCyc:4OHBENZOATE-OCTAPRENYLTRANSFER-MONOMER.
ECOL316407:JW4000-MONOMER.
MetaCyc:4OHBENZOATE-OCTAPRENYLTRANSFER-MONOMER.

Miscellaneous databases

PROiP0AGK1.

Family and domain databases

HAMAPiMF_01635. UbiA. 1 hit.
InterProiIPR031103. HB_octoprenylTrfase.
IPR006370. HB_polyprenyltransferase.
IPR000537. UbiA_prenyltransferase.
IPR030470. UbiA_prenylTrfase_CS.
[Graphical view]
PfamiPF01040. UbiA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01474. ubiA_proteo. 1 hit.
PROSITEiPS00943. UBIA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBIA_ECOLI
AccessioniPrimary (citable) accession number: P0AGK1
Secondary accession number(s): P26601, Q2M6R4, Q4JHR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: September 7, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.