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Protein

Tyrosine--tRNA ligase

Gene

tyrS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).

Catalytic activityi

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37Tyrosine1 Publication1
Binding sitei175Tyrosine1 Publication1
Binding sitei179Tyrosine1 Publication1
Binding sitei198Tyr-AMP intermediate adenyl group; via amide nitrogen1
Binding sitei200Tyr-AMP intermediate adenyl group1
Binding sitei228Tyr-AMP intermediate adenyl group; via amide nitrogen and carbonyl oxygen1
Sitei231Cross-linked with tRNA by periodate oxidation1
Sitei235Cross-linked with tRNA by periodate oxidation; predominant1
Binding sitei238ATPBy similarity1
Sitei238Cross-linked with tRNA by periodate oxidation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • RNA binding Source: UniProtKB-KW
  • tyrosine-tRNA ligase activity Source: EcoCyc

GO - Biological processi

  • tyrosyl-tRNA aminoacylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:TYRS-MONOMER.
ECOL316407:JW1629-MONOMER.
MetaCyc:TYRS-MONOMER.
BRENDAi6.1.1.1. 2026.
SABIO-RKP0AGJ9.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine--tRNA ligase (EC:6.1.1.1)
Alternative name(s):
Tyrosyl-tRNA synthetase
Short name:
TyrRS
Gene namesi
Name:tyrS
Ordered Locus Names:b1637, JW1629
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11043. tyrS.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi37Y → V: Confers specificity for the non-natural amino acid 3-iodo-tyrosine; when associated with C-195. 1
Mutagenesisi195Q → C: Confers specificity for the non-natural amino acid 3-iodo-tyrosine; when associated with V-37. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000556522 – 424Tyrosine--tRNA ligaseAdd BLAST423

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei144N6-acetyllysine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP0AGJ9.
PaxDbiP0AGJ9.
PRIDEiP0AGJ9.

2D gel databases

SWISS-2DPAGEP0AGJ9.

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

BioGridi4263487. 17 interactors.
DIPiDIP-36227N.
IntActiP0AGJ9. 8 interactors.
MINTiMINT-1224558.
STRINGi511145.b1637.

Structurei

Secondary structure

1424
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 12Combined sources7
Beta strandi17 – 21Combined sources5
Helixi22 – 31Combined sources10
Beta strandi35 – 40Combined sources6
Beta strandi43 – 46Combined sources4
Helixi49 – 63Combined sources15
Beta strandi67 – 72Combined sources6
Helixi76 – 78Combined sources3
Helixi94 – 108Combined sources15
Helixi109 – 111Combined sources3
Beta strandi114 – 116Combined sources3
Helixi117 – 119Combined sources3
Beta strandi122 – 125Combined sources4
Helixi127 – 130Combined sources4
Helixi135 – 141Combined sources7
Helixi143 – 145Combined sources3
Helixi148 – 151Combined sources4
Helixi155 – 162Combined sources8
Helixi164 – 166Combined sources3
Helixi170 – 173Combined sources4
Helixi175 – 190Combined sources16
Beta strandi192 – 197Combined sources6
Helixi199 – 201Combined sources3
Helixi202 – 216Combined sources15
Beta strandi221 – 224Combined sources4
Beta strandi240 – 242Combined sources3
Beta strandi245 – 247Combined sources3
Turni248 – 250Combined sources3
Helixi253 – 261Combined sources9
Helixi265 – 275Combined sources11
Helixi280 – 293Combined sources14
Helixi299 – 319Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VBMX-ray2.70A/B5-322[»]
1VBNX-ray2.70A/B5-322[»]
1WQ3X-ray2.00A1-322[»]
1WQ4X-ray2.00A2-322[»]
1X8XX-ray2.00A1-322[»]
2YXNX-ray1.80A1-322[»]
ProteinModelPortaliP0AGJ9.
SMRiP0AGJ9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AGJ9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini357 – 414S4 RNA-bindingAdd BLAST58

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi42 – 51"HIGH" region10
Motifi235 – 239"KMSKS" region5

Sequence similaritiesi

Contains 1 S4 RNA-binding domain.Curated

Phylogenomic databases

eggNOGiENOG4105DA0. Bacteria.
COG0162. LUCA.
HOGENOMiHOG000242790.
InParanoidiP0AGJ9.
KOiK01866.
OMAiGKHFPVN.
PhylomeDBiP0AGJ9.

Family and domain databases

CDDicd00805. TyrRS_core. 1 hit.
Gene3Di3.10.290.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_02006. Tyr_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002942. S4_RNA-bd.
IPR002307. Tyr-tRNA-ligase.
IPR024088. Tyr-tRNA-ligase_bac-type.
IPR024107. Tyr-tRNA-ligase_bac_1.
[Graphical view]
PANTHERiPTHR11766. PTHR11766. 1 hit.
PfamiPF01479. S4. 1 hit.
PF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01040. TRNASYNTHTYR.
SMARTiSM00363. S4. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00234. tyrS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AGJ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSNLIKQL QERGLVAQVT DEEALAERLA QGPIALYCGF DPTADSLHLG
60 70 80 90 100
HLVPLLCLKR FQQAGHKPVA LVGGATGLIG DPSFKAAERK LNTEETVQEW
110 120 130 140 150
VDKIRKQVAP FLDFDCGENS AIAANNYDWF GNMNVLTFLR DIGKHFSVNQ
160 170 180 190 200
MINKEAVKQR LNREDQGISF TEFSYNLLQG YDFACLNKQY GVVLQIGGSD
210 220 230 240 250
QWGNITSGID LTRRLHQNQV FGLTVPLITK ADGTKFGKTE GGAVWLDPKK
260 270 280 290 300
TSPYKFYQFW INTADADVYR FLKFFTFMSI EEINALEEED KNSGKAPRAQ
310 320 330 340 350
YVLAEQVTRL VHGEEGLQAA KRITECLFSG SLSALSEADF EQLAQDGVPM
360 370 380 390 400
VEMEKGADLM QALVDSELQP SRGQARKTIA SNAITINGEK QSDPEYFFKE
410 420
EDRLFGRFTL LRRGKKNYCL ICWK
Length:424
Mass (Da):47,527
Last modified:January 23, 2007 - v2
Checksum:i98E7080D85B356A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01719 Genomic DNA. Translation: AAA24707.1.
U00096 Genomic DNA. Translation: AAC74709.1.
AP009048 Genomic DNA. Translation: BAA15398.1.
M92351 Genomic DNA. Translation: AAA24710.1.
PIRiA01178. SYECYT.
RefSeqiNP_416154.1. NC_000913.3.
WP_001295400.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74709; AAC74709; b1637.
BAA15398; BAA15398; BAA15398.
GeneIDi948855.
KEGGiecj:JW1629.
eco:b1637.
PATRICi32118576. VBIEscCol129921_1708.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01719 Genomic DNA. Translation: AAA24707.1.
U00096 Genomic DNA. Translation: AAC74709.1.
AP009048 Genomic DNA. Translation: BAA15398.1.
M92351 Genomic DNA. Translation: AAA24710.1.
PIRiA01178. SYECYT.
RefSeqiNP_416154.1. NC_000913.3.
WP_001295400.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VBMX-ray2.70A/B5-322[»]
1VBNX-ray2.70A/B5-322[»]
1WQ3X-ray2.00A1-322[»]
1WQ4X-ray2.00A2-322[»]
1X8XX-ray2.00A1-322[»]
2YXNX-ray1.80A1-322[»]
ProteinModelPortaliP0AGJ9.
SMRiP0AGJ9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263487. 17 interactors.
DIPiDIP-36227N.
IntActiP0AGJ9. 8 interactors.
MINTiMINT-1224558.
STRINGi511145.b1637.

2D gel databases

SWISS-2DPAGEP0AGJ9.

Proteomic databases

EPDiP0AGJ9.
PaxDbiP0AGJ9.
PRIDEiP0AGJ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74709; AAC74709; b1637.
BAA15398; BAA15398; BAA15398.
GeneIDi948855.
KEGGiecj:JW1629.
eco:b1637.
PATRICi32118576. VBIEscCol129921_1708.

Organism-specific databases

EchoBASEiEB1036.
EcoGeneiEG11043. tyrS.

Phylogenomic databases

eggNOGiENOG4105DA0. Bacteria.
COG0162. LUCA.
HOGENOMiHOG000242790.
InParanoidiP0AGJ9.
KOiK01866.
OMAiGKHFPVN.
PhylomeDBiP0AGJ9.

Enzyme and pathway databases

BioCyciEcoCyc:TYRS-MONOMER.
ECOL316407:JW1629-MONOMER.
MetaCyc:TYRS-MONOMER.
BRENDAi6.1.1.1. 2026.
SABIO-RKP0AGJ9.

Miscellaneous databases

EvolutionaryTraceiP0AGJ9.
PROiP0AGJ9.

Family and domain databases

CDDicd00805. TyrRS_core. 1 hit.
Gene3Di3.10.290.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_02006. Tyr_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002942. S4_RNA-bd.
IPR002307. Tyr-tRNA-ligase.
IPR024088. Tyr-tRNA-ligase_bac-type.
IPR024107. Tyr-tRNA-ligase_bac_1.
[Graphical view]
PANTHERiPTHR11766. PTHR11766. 1 hit.
PfamiPF01479. S4. 1 hit.
PF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01040. TRNASYNTHTYR.
SMARTiSM00363. S4. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00234. tyrS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50889. S4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYY_ECOLI
AccessioniPrimary (citable) accession number: P0AGJ9
Secondary accession number(s): P00951
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.