Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA (cytidine(34)-2'-O)-methyltransferase

Gene

trmL

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide. Recognition of the target requires a pyridine at position 34 and N6-(isopentenyl)-2-methylthioadenosine at position 37.UniRule annotation1 Publication

Caution

Possibly identical to GltE.Curated

Catalytic activityi

S-adenosyl-L-methionine + cytidine(34) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(34) in tRNA.UniRule annotation1 Publication
S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine(34) in tRNA(Leu) = S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine(34) in tRNA(Leu).UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei78S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei100S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1
Binding sitei122S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei130S-adenosyl-L-methionineUniRule annotation1

GO - Molecular functioni

  • RNA binding Source: InterPro
  • tRNA (cytosine-2'-O-)-methyltransferase activity Source: EcoCyc
  • tRNA (uracil-2'-O-)-methyltransferase activity Source: EcoCyc

GO - Biological processi

  • wobble position cytosine ribose methylation Source: EcoCyc
  • wobble position uridine ribose methylation Source: EcoCyc

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG11888-MONOMER
MetaCyc:EG11888-MONOMER
BRENDAi2.1.1.207 2026

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cytidine(34)-2'-O)-methyltransferaseUniRule annotation (EC:2.1.1.207UniRule annotation)
Alternative name(s):
tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmLUniRule annotation
Gene namesi
Name:trmLUniRule annotation
Synonyms:yibK
Ordered Locus Names:b3606, JW3581
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11888 trmL

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001598171 – 157tRNA (cytidine(34)-2'-O)-methyltransferaseAdd BLAST157

Proteomic databases

PaxDbiP0AGJ7
PRIDEiP0AGJ7

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4262552, 53 interactors
IntActiP0AGJ7, 7 interactors
STRINGi316385.ECDH10B_3788

Structurei

Secondary structure

1157
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi12 – 25Combined sources14
Beta strandi28 – 33Combined sources6
Helixi42 – 44Combined sources3
Helixi51 – 56Combined sources6
Beta strandi57 – 62Combined sources6
Helixi63 – 70Combined sources8
Beta strandi73 – 78Combined sources6
Beta strandi83 – 85Combined sources3
Turni86 – 88Combined sources3
Beta strandi95 – 99Combined sources5
Turni102 – 104Combined sources3
Helixi108 – 111Combined sources4
Helixi116 – 118Combined sources3
Beta strandi119 – 121Combined sources3
Helixi133 – 147Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4JAKX-ray2.00A/B2-157[»]
4JALX-ray2.00A/B2-157[»]
ProteinModelPortaliP0AGJ7
SMRiP0AGJ7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. TrmL subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108UIQ Bacteria
COG0219 LUCA
HOGENOMiHOG000272757
InParanoidiP0AGJ7
KOiK03216
OMAiAGLDYWH
PhylomeDBiP0AGJ7

Family and domain databases

Gene3Di3.40.1280.10, 1 hit
HAMAPiMF_01885 tRNA_methyltr_TrmL, 1 hit
InterProiView protein in InterPro
IPR029028 Alpha/beta_knot_MTases
IPR001537 SpoU_MeTrfase
IPR016914 tRNA_cyt/urid_MeTfrase
IPR029026 tRNA_m1G_MTases_N
PANTHERiPTHR42971 PTHR42971, 1 hit
PfamiView protein in Pfam
PF00588 SpoU_methylase, 1 hit
PIRSFiPIRSF029256 SpoU_TrmH_prd, 1 hit
SUPFAMiSSF75217 SSF75217, 1 hit
TIGRFAMsiTIGR00185 tRNA_yibK_trmL, 1 hit

Sequencei

Sequence statusi: Complete.

P0AGJ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNIVLYEPE IPPNTGNIIR LCANTGFRLH IIEPMGFAWD DKRLRRAGLD
60 70 80 90 100
YHEFTAVTRH HDYRAFLEAE NPQRLFALTT KGTPAHSAVS YQDGDYLMFG
110 120 130 140 150
PETRGLPASI LDALPAEQKI RIPMVPDSRS MNLSNAVSVV VYEAWRQLGY

PGAVLRD
Length:157
Mass (Da):17,726
Last modified:December 20, 2005 - v1
Checksum:iBF065D29B86C341B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13970 Unassigned DNA No translation available.
U00039 Genomic DNA Translation: AAB18583.1
U00096 Genomic DNA Translation: AAC76630.1
AP009048 Genomic DNA Translation: BAE77686.1
M34333 Genomic DNA No translation available.
PIRiS47827
RefSeqiNP_418063.1, NC_000913.3
WP_000932347.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76630; AAC76630; b3606
BAE77686; BAE77686; BAE77686
GeneIDi948119
KEGGiecj:JW3581
eco:b3606
PATRICifig|1411691.4.peg.3100

Entry informationi

Entry nameiTRML_ECOLI
AccessioniPrimary (citable) accession number: P0AGJ7
Secondary accession number(s): P33899, Q2M7S0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health