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Protein

tRNA (cytidine(34)-2'-O)-methyltransferase

Gene

trmL

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide. Recognition of the target requires a pyridine at position 34 and N(6)-(isopentenyl)-2-methylthioadenosine at position 37.UniRule annotation1 Publication

Catalytic activityi

S-adenosyl-L-methionine + cytidine(34) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(34) in tRNA.UniRule annotation1 Publication
S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine(34) in tRNA(Leu) = S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine(34) in tRNA(Leu).UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei78S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei100S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1
Binding sitei122S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei130S-adenosyl-L-methionineUniRule annotation1

GO - Molecular functioni

  • RNA binding Source: GO_Central
  • tRNA (cytosine-2'-O-)-methyltransferase activity Source: EcoCyc
  • tRNA (uracil-2'-O-)-methyltransferase activity Source: EcoCyc

GO - Biological processi

  • wobble position cytosine ribose methylation Source: EcoCyc
  • wobble position uridine ribose methylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG11888-MONOMER.
ECOL316407:JW3581-MONOMER.
MetaCyc:EG11888-MONOMER.
BRENDAi2.1.1.207. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cytidine(34)-2'-O)-methyltransferaseUniRule annotation (EC:2.1.1.207UniRule annotation)
Alternative name(s):
tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmLUniRule annotation
Gene namesi
Name:trmLUniRule annotation
Synonyms:yibK
Ordered Locus Names:b3606, JW3581
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11888. trmL.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001598171 – 157tRNA (cytidine(34)-2'-O)-methyltransferaseAdd BLAST157

Proteomic databases

PaxDbiP0AGJ7.
PRIDEiP0AGJ7.

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4262552. 10 interactors.
IntActiP0AGJ7. 7 interactors.
MINTiMINT-1308881.
STRINGi511145.b3606.

Structurei

Secondary structure

1157
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi12 – 25Combined sources14
Beta strandi28 – 33Combined sources6
Helixi42 – 44Combined sources3
Helixi51 – 56Combined sources6
Beta strandi57 – 62Combined sources6
Helixi63 – 70Combined sources8
Beta strandi73 – 78Combined sources6
Beta strandi83 – 85Combined sources3
Turni86 – 88Combined sources3
Beta strandi95 – 99Combined sources5
Turni102 – 104Combined sources3
Helixi108 – 111Combined sources4
Helixi116 – 118Combined sources3
Beta strandi119 – 121Combined sources3
Helixi133 – 147Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4JAKX-ray2.00A/B2-157[»]
4JALX-ray2.00A/B2-157[»]
ProteinModelPortaliP0AGJ7.
SMRiP0AGJ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. TrmL subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108UIQ. Bacteria.
COG0219. LUCA.
HOGENOMiHOG000272757.
InParanoidiP0AGJ7.
KOiK03216.
OMAiAGLDYWH.
PhylomeDBiP0AGJ7.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
HAMAPiMF_01885. tRNA_methyltr_TrmL. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR001537. SpoU_MeTrfase.
IPR016914. tRNA_cyt/urid_MeTfrase.
IPR029026. tRNA_m1G_MTases_N.
[Graphical view]
PfamiPF00588. SpoU_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF029256. SpoU_TrmH_prd. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00185. tRNA_yibK_trmL. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AGJ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNIVLYEPE IPPNTGNIIR LCANTGFRLH IIEPMGFAWD DKRLRRAGLD
60 70 80 90 100
YHEFTAVTRH HDYRAFLEAE NPQRLFALTT KGTPAHSAVS YQDGDYLMFG
110 120 130 140 150
PETRGLPASI LDALPAEQKI RIPMVPDSRS MNLSNAVSVV VYEAWRQLGY

PGAVLRD
Length:157
Mass (Da):17,726
Last modified:December 20, 2005 - v1
Checksum:iBF065D29B86C341B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13970 Unassigned DNA. No translation available.
U00039 Genomic DNA. Translation: AAB18583.1.
U00096 Genomic DNA. Translation: AAC76630.1.
AP009048 Genomic DNA. Translation: BAE77686.1.
M34333 Genomic DNA. No translation available.
PIRiS47827.
RefSeqiNP_418063.1. NC_000913.3.
WP_000932347.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76630; AAC76630; b3606.
BAE77686; BAE77686; BAE77686.
GeneIDi948119.
KEGGiecj:JW3581.
eco:b3606.
PATRICi32122697. VBIEscCol129921_3725.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13970 Unassigned DNA. No translation available.
U00039 Genomic DNA. Translation: AAB18583.1.
U00096 Genomic DNA. Translation: AAC76630.1.
AP009048 Genomic DNA. Translation: BAE77686.1.
M34333 Genomic DNA. No translation available.
PIRiS47827.
RefSeqiNP_418063.1. NC_000913.3.
WP_000932347.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4JAKX-ray2.00A/B2-157[»]
4JALX-ray2.00A/B2-157[»]
ProteinModelPortaliP0AGJ7.
SMRiP0AGJ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262552. 10 interactors.
IntActiP0AGJ7. 7 interactors.
MINTiMINT-1308881.
STRINGi511145.b3606.

Proteomic databases

PaxDbiP0AGJ7.
PRIDEiP0AGJ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76630; AAC76630; b3606.
BAE77686; BAE77686; BAE77686.
GeneIDi948119.
KEGGiecj:JW3581.
eco:b3606.
PATRICi32122697. VBIEscCol129921_3725.

Organism-specific databases

EchoBASEiEB1834.
EcoGeneiEG11888. trmL.

Phylogenomic databases

eggNOGiENOG4108UIQ. Bacteria.
COG0219. LUCA.
HOGENOMiHOG000272757.
InParanoidiP0AGJ7.
KOiK03216.
OMAiAGLDYWH.
PhylomeDBiP0AGJ7.

Enzyme and pathway databases

BioCyciEcoCyc:EG11888-MONOMER.
ECOL316407:JW3581-MONOMER.
MetaCyc:EG11888-MONOMER.
BRENDAi2.1.1.207. 2026.

Miscellaneous databases

PROiP0AGJ7.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
HAMAPiMF_01885. tRNA_methyltr_TrmL. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR001537. SpoU_MeTrfase.
IPR016914. tRNA_cyt/urid_MeTfrase.
IPR029026. tRNA_m1G_MTases_N.
[Graphical view]
PfamiPF00588. SpoU_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF029256. SpoU_TrmH_prd. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
TIGRFAMsiTIGR00185. tRNA_yibK_trmL. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRML_ECOLI
AccessioniPrimary (citable) accession number: P0AGJ7
Secondary accession number(s): P33899, Q2M7S0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Possibly identical to GltE.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.