P0AGJ7 (TRML_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (cytidine(34)-2'-O)-methyltransferase
EC=2.1.1.207

Alternative name(s):
tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmL

Gene names

Name:	trmL
Synonyms:	yibK
Ordered Locus Names:	b3606, JW3581

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide. Recognition of the target requires a pyridine at position 34 and N(6)-(isopentenyl)-2-methylthioadenosine at position 37. Ref.7
Catalytic activity	S-adenosyl-L-methionine + cytidine34 in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine34 in tRNA. Ref.7 S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine34 in tRNA(Leu) = S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine34 in tRNA(Leu). Ref.7
Subunit structure	Homodimer. Ref.7
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01885.
Sequence similarities	Belongs to the RNA methyltransferase TrmH family. TrmL subfamily.
Caution	Possibly identical to GltE.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	wobble position cytosine ribose methylation Inferred from mutant phenotype Ref.7. Source: EcoCyc wobble position uridine ribose methylation Inferred from mutant phenotype Ref.7. Source: EcoCyc
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (cytosine-2'-O-)-methyltransferase activity Inferred from direct assay Ref.7. Source: EcoCyc tRNA (uracil-2'-O-)-methyltransferase activity Inferred from direct assay Ref.7. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 157	157	tRNA (cytidine(34)-2'-O)-methyltransferase HAMAP-Rule MF_01885		PRO_0000159817
Sites
	Binding site	78	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	100	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	122	1	S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	130	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0AGJ7 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: BF065D29B86C341B	FASTA	157	17,726
	10 20 30 40 50 60 MLNIVLYEPE IPPNTGNIIR LCANTGFRLH IIEPMGFAWD DKRLRRAGLD YHEFTAVTRH 70 80 90 100 110 120 HDYRAFLEAE NPQRLFALTT KGTPAHSAVS YQDGDYLMFG PETRGLPASI LDALPAEQKI 130 140 150 RIPMVPDSRS MNLSNAVSVV VYEAWRQLGY PGAVLRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Three overlapping lct genes involved in L-lactate utilization by Escherichia coli." Dong J.M., Taylor J.S., Latour D.J., Iuchi S., Lin E.C.C. J. Bacteriol. 175:6671-6678(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Structure and expression of cysX, the second gene in the Escherichia coli K-12 cysE locus." Tei H., Murata K., Kimura A. Biochem. Biophys. Res. Commun. 167:948-955(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 73-157. Strain: K12.
[6]	"SpoU protein of Escherichia coli belongs to a new family of putative rRNA methylases." Koonin E.V., Rudd K.E. Nucleic Acids Res. 21:5519-5519(1993) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[7]	"YibK is the 2'-O-methyltransferase TrmL that modifies the wobble nucleotide in Escherichia coli tRNALeu isoacceptors." Benitez-Paez A., Villarroya M., Douthwaite S., Gabaldon T., Armengod M.E. RNA 16:2131-2143(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS A METHYLTRANSFERASE, CATALYTIC ACTIVITY, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L13970 Unassigned DNA. No translation available. U00039 Genomic DNA. Translation: AAB18583.1. U00096 Genomic DNA. Translation: AAC76630.1. AP009048 Genomic DNA. Translation: BAE77686.1. M34333 Genomic DNA. No translation available.
PIR	S47827.
RefSeq	NP_418063.1. NC_000913.2. YP_491827.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0AGJ7.
SMR	P0AGJ7. Positions 1-155.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0AGJ7. 7 interactions.
MINT	MINT-1308881.
STRING	511145.b3606.
Proteomic databases
PaxDb	P0AGJ7.
PRIDE	P0AGJ7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76630; AAC76630; b3606. BAE77686; BAE77686; BAE77686.
GeneID	12933558. 948119.
KEGG	ecj:Y75_p3568. eco:b3606.
PATRIC	32122697. VBIEscCol129921_3725.
Organism-specific databases
EchoBASE	EB1834.
EcoGene	EG11888. trmL.
Phylogenomic databases
eggNOG	COG0219.
HOGENOM	HOG000272757.
KO	K03216.
OMA	EAWRQNG.
ProtClustDB	PRK10358.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11888-MONOMER. ECOL316407:JW3581-MONOMER. MetaCyc:EG11888-MONOMER.
Gene expression databases
Genevestigator	P0AGJ7.
Family and domain databases
HAMAP	MF_01885. tRNA_methyltr_TrmL.
InterPro	IPR001537. SpoU_MeTrfase. IPR016914. tRNA_cyt/urid_MeTfrase. [Graphical view]
Pfam	PF00588. SpoU_methylase. 1 hit. [Graphical view]
PIRSF	PIRSF029256. SpoU_TrmH_prd. 1 hit.
TIGRFAMs	TIGR00185. tRNA_yibK_trmL. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRML_ECOLI

Accession

Primary (citable) accession number: P0AGJ7
Secondary accession number(s): P33899, Q2M7S0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program