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Protein

Uncharacterized tRNA/rRNA methyltransferase YfiF

Gene

yfiF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:EG11786-MONOMER.
ECOL316407:JW2565-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Uncharacterized tRNA/rRNA methyltransferase YfiF (EC:2.1.1.-)
Gene namesi
Name:yfiF
Ordered Locus Names:b2581, JW2565
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11786. yfiF.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001598141 – 345Uncharacterized tRNA/rRNA methyltransferase YfiFAdd BLAST345

Proteomic databases

EPDiP0AGJ5.
PaxDbiP0AGJ5.
PRIDEiP0AGJ5.

Interactioni

Protein-protein interaction databases

BioGridi4263473. 10 interactors.
DIPiDIP-47835N.
IntActiP0AGJ5. 75 interactors.
MINTiMINT-1218417.
STRINGi511145.b2581.

Structurei

3D structure databases

ProteinModelPortaliP0AGJ5.
SMRiP0AGJ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105EIB. Bacteria.
COG0566. LUCA.
HOGENOMiHOG000218800.
InParanoidiP0AGJ5.
KOiK03214.
OMAiIVRLWAT.
PhylomeDBiP0AGJ5.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
3.40.1280.10. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR029064. L30e-like.
IPR001537. SpoU_MeTrfase.
IPR013123. SpoU_subst-bd.
IPR029026. tRNA_m1G_MTases_N.
IPR016479. YfiF_prd.
[Graphical view]
PfamiPF00588. SpoU_methylase. 1 hit.
PF08032. SpoU_sub_bind. 1 hit.
[Graphical view]
PIRSFiPIRSF006280. YfiF_prd. 1 hit.
SMARTiSM00967. SpoU_sub_bind. 1 hit.
[Graphical view]
SUPFAMiSSF55315. SSF55315. 1 hit.
SSF75217. SSF75217. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AGJ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDEMKGKSG KVKVMYVRSD DDSDKRTHNP RTGKGGGRPG KSRADGGRRP
60 70 80 90 100
ARDDKQSQPR DRKWEDSPWR TVSRAPGDET PEKADHGGIS GKSFIDPEVL
110 120 130 140 150
RRQRAEETRV YGENACQALF QSRPEAIVRA WFIQSVTPRF KEALRWMAAN
160 170 180 190 200
RKAYHVVDEA ELTKASGTEH HGGVCFLIKK RNGTTVQQWV SQAGAQDCVL
210 220 230 240 250
ALENESNPHN LGGMMRSCAH FGVKGVVVQD AALLESGAAI RTAEGGAEHV
260 270 280 290 300
QPITGDNIVN VLDDFRQAGY TVVTTSSEQG KPLFKTSLPA KMVLVLGQEY
310 320 330 340
EGLPDAARDP NDLRVKIDGT GNVAGLNISV ATGVLLGEWW RQNKA
Length:345
Mass (Da):37,784
Last modified:December 20, 2005 - v1
Checksum:i9845E9884D8369BF
GO

Sequence cautioni

The sequence D13169 differs from that shown. Reason: Frameshift at position 331.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13169 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC75634.1.
AP009048 Genomic DNA. Translation: BAA16467.2.
J03725 Genomic DNA. No translation available.
PIRiD65036.
RefSeqiNP_417076.1. NC_000913.3.
WP_000997403.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75634; AAC75634; b2581.
BAA16467; BAA16467; BAA16467.
GeneIDi947066.
KEGGiecj:JW2565.
eco:b2581.
PATRICi32120563. VBIEscCol129921_2684.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13169 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC75634.1.
AP009048 Genomic DNA. Translation: BAA16467.2.
J03725 Genomic DNA. No translation available.
PIRiD65036.
RefSeqiNP_417076.1. NC_000913.3.
WP_000997403.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AGJ5.
SMRiP0AGJ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263473. 10 interactors.
DIPiDIP-47835N.
IntActiP0AGJ5. 75 interactors.
MINTiMINT-1218417.
STRINGi511145.b2581.

Proteomic databases

EPDiP0AGJ5.
PaxDbiP0AGJ5.
PRIDEiP0AGJ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75634; AAC75634; b2581.
BAA16467; BAA16467; BAA16467.
GeneIDi947066.
KEGGiecj:JW2565.
eco:b2581.
PATRICi32120563. VBIEscCol129921_2684.

Organism-specific databases

EchoBASEiEB1734.
EcoGeneiEG11786. yfiF.

Phylogenomic databases

eggNOGiENOG4105EIB. Bacteria.
COG0566. LUCA.
HOGENOMiHOG000218800.
InParanoidiP0AGJ5.
KOiK03214.
OMAiIVRLWAT.
PhylomeDBiP0AGJ5.

Enzyme and pathway databases

BioCyciEcoCyc:EG11786-MONOMER.
ECOL316407:JW2565-MONOMER.

Miscellaneous databases

PROiP0AGJ5.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
3.40.1280.10. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR029064. L30e-like.
IPR001537. SpoU_MeTrfase.
IPR013123. SpoU_subst-bd.
IPR029026. tRNA_m1G_MTases_N.
IPR016479. YfiF_prd.
[Graphical view]
PfamiPF00588. SpoU_methylase. 1 hit.
PF08032. SpoU_sub_bind. 1 hit.
[Graphical view]
PIRSFiPIRSF006280. YfiF_prd. 1 hit.
SMARTiSM00967. SpoU_sub_bind. 1 hit.
[Graphical view]
SUPFAMiSSF55315. SSF55315. 1 hit.
SSF75217. SSF75217. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiYFIF_ECOLI
AccessioniPrimary (citable) accession number: P0AGJ5
Secondary accession number(s): P33635, P76999
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.