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Protein

Trk system potassium uptake protein TrkA

Gene

trkA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the constitutive potassium transport systems TrkG and TrkH. May regulate the transport activity of TrkG and TrkH systems. Binds to NAD+ and NADH.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei30 – 301NAD 1By similarity
Binding sitei98 – 981NAD 1; shared with dimeric partnerBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi7 – 115NAD 1By similarity
Nucleotide bindingi73 – 742NAD 1By similarity
Nucleotide bindingi234 – 26229NAD 2Sequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • potassium ion transport Source: EcoCyc
  • secondary metabolite biosynthetic process Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

NAD, Potassium

Enzyme and pathway databases

BioCyciEcoCyc:TRKA-MONOMER.
ECOL316407:JW3251-MONOMER.

Protein family/group databases

TCDBi2.A.38.1.1. the k(+) transporter (trk) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Trk system potassium uptake protein TrkA
Short name:
K(+)-uptake protein TrkA
Gene namesi
Name:trkA
Ordered Locus Names:b3290, JW3251
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11019. trkA.

Subcellular locationi

  • Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication

  • Note: Peripherally bound to the inner side of the inner membrane via the TrkG and TrkH proteins.

GO - Cellular componenti

  • membrane Source: EcoCyc
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 458458Trk system potassium uptake protein TrkAPRO_0000148712Add
BLAST

Proteomic databases

PaxDbiP0AGI8.
PRIDEiP0AGI8.

Interactioni

Protein-protein interaction databases

BioGridi4263438. 8 interactions.
DIPiDIP-35971N.
IntActiP0AGI8. 4 interactions.
STRINGi511145.b3290.

Structurei

3D structure databases

ProteinModelPortaliP0AGI8.
SMRiP0AGI8. Positions 1-457.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 131130RCK N-terminal 1PROSITE-ProRule annotationAdd
BLAST
Domaini143 – 22785RCK C-terminal 1PROSITE-ProRule annotationAdd
BLAST
Domaini234 – 356123RCK N-terminal 2PROSITE-ProRule annotationAdd
BLAST
Domaini368 – 45386RCK C-terminal 2PROSITE-ProRule annotationAdd
BLAST

Domaini

The RCK N-terminal domain binds NAD and possibly other effectors. This is expected to cause a conformation change that regulates potassium transport (By similarity).By similarity

Sequence similaritiesi

Contains 2 RCK C-terminal domains.PROSITE-ProRule annotation
Contains 2 RCK N-terminal domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105C1A. Bacteria.
COG0569. LUCA.
HOGENOMiHOG000227130.
InParanoidiP0AGI8.
KOiK03499.
OMAiIACQVAY.
PhylomeDBiP0AGI8.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR006036. K_uptake_TrkA.
IPR016040. NAD(P)-bd_dom.
IPR006037. RCK_C.
IPR003148. RCK_N.
[Graphical view]
PfamiPF02080. TrkA_C. 2 hits.
PF02254. TrkA_N. 2 hits.
[Graphical view]
PRINTSiPR00335. KUPTAKETRKA.
SUPFAMiSSF116726. SSF116726. 2 hits.
SSF51735. SSF51735. 2 hits.
PROSITEiPS51202. RCK_C. 2 hits.
PS51201. RCK_N. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AGI8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIIILGAGQ VGGTLAENLV GENNDITVVD TNGERLRTLQ DKFDLRVVQG
60 70 80 90 100
HGSHPRVLRE AGADDADMLV AVTSSDETNM VACQVAYSLF NTPNRIARIR
110 120 130 140 150
SPDYVRDADK LFHSDAVPID HLIAPEQLVI DNIYRLIEYP GALQVVNFAE
160 170 180 190 200
GKVSLAVVKA YYGGPLIGNA LSTMREHMPH IDTRVAAIFR HDRPIRPQGS
210 220 230 240 250
TIVEAGDEVF FIAASQHIRA VMSELQRLEK PYKRIMLVGG GNIGAGLARR
260 270 280 290 300
LEKDYSVKLI ERNQQRAAEL AEKLQNTIVF FGDASDQELL AEEHIDQVDL
310 320 330 340 350
FIAVTNDDEA NIMSAMLAKR MGAKKVMVLI QRRAYVDLVQ GSVIDIAISP
360 370 380 390 400
QQATISALLS HVRKADIVGV SSLRRGVAEA IEAVAHGDES TSRVVGRVID
410 420 430 440 450
EIKLPPGTII GAVVRGNDVM IANDNLRIEQ GDHVIMFLTD KKFITDVERL

FQPSPFFL
Length:458
Mass (Da):50,368
Last modified:December 20, 2005 - v1
Checksum:i1FF01BC23F4DC40F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52114 Genomic DNA. Translation: CAA36359.1.
X77091 Genomic DNA. Translation: CAA54371.1.
U18997 Genomic DNA. Translation: AAA58087.1.
U00096 Genomic DNA. Translation: AAC76315.1.
AP009048 Genomic DNA. Translation: BAE78002.1.
Y10307 Genomic DNA. Translation: CAA71360.1.
PIRiS36252.
RefSeqiNP_417748.1. NC_000913.3.
WP_000691382.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76315; AAC76315; b3290.
BAE78002; BAE78002; BAE78002.
GeneIDi947788.
KEGGiecj:JW3251.
eco:b3290.
PATRICi32122012. VBIEscCol129921_3383.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52114 Genomic DNA. Translation: CAA36359.1.
X77091 Genomic DNA. Translation: CAA54371.1.
U18997 Genomic DNA. Translation: AAA58087.1.
U00096 Genomic DNA. Translation: AAC76315.1.
AP009048 Genomic DNA. Translation: BAE78002.1.
Y10307 Genomic DNA. Translation: CAA71360.1.
PIRiS36252.
RefSeqiNP_417748.1. NC_000913.3.
WP_000691382.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AGI8.
SMRiP0AGI8. Positions 1-457.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263438. 8 interactions.
DIPiDIP-35971N.
IntActiP0AGI8. 4 interactions.
STRINGi511145.b3290.

Protein family/group databases

TCDBi2.A.38.1.1. the k(+) transporter (trk) family.

Proteomic databases

PaxDbiP0AGI8.
PRIDEiP0AGI8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76315; AAC76315; b3290.
BAE78002; BAE78002; BAE78002.
GeneIDi947788.
KEGGiecj:JW3251.
eco:b3290.
PATRICi32122012. VBIEscCol129921_3383.

Organism-specific databases

EchoBASEiEB1012.
EcoGeneiEG11019. trkA.

Phylogenomic databases

eggNOGiENOG4105C1A. Bacteria.
COG0569. LUCA.
HOGENOMiHOG000227130.
InParanoidiP0AGI8.
KOiK03499.
OMAiIACQVAY.
PhylomeDBiP0AGI8.

Enzyme and pathway databases

BioCyciEcoCyc:TRKA-MONOMER.
ECOL316407:JW3251-MONOMER.

Miscellaneous databases

PROiP0AGI8.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR006036. K_uptake_TrkA.
IPR016040. NAD(P)-bd_dom.
IPR006037. RCK_C.
IPR003148. RCK_N.
[Graphical view]
PfamiPF02080. TrkA_C. 2 hits.
PF02254. TrkA_N. 2 hits.
[Graphical view]
PRINTSiPR00335. KUPTAKETRKA.
SUPFAMiSSF116726. SSF116726. 2 hits.
SSF51735. SSF51735. 2 hits.
PROSITEiPS51202. RCK_C. 2 hits.
PS51201. RCK_N. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRKA_ECOLI
AccessioniPrimary (citable) accession number: P0AGI8
Secondary accession number(s): P23868, P77041, Q2M6V4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: September 7, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.