P0AGH8 (PSTC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphate transport system permease protein PstC

Gene names

Name:	pstC
Synonyms:	phoW
Ordered Locus Names:	b3727, JW3705

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily. Contains 1 ABC transmembrane type-1 domain.

Ontologies

Keywords
Biological process	Phosphate transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	inorganic phosphate transmembrane transporter activity Inferred from electronic annotation. Source: InterPro phosphate ion transmembrane-transporting ATPase activity Inferred from direct assay PubMed 7026529. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	Phosphate transport system permease protein PstC		PRO_0000060207
Regions
	Topological domain	1 – 24	24	Cytoplasmic Potential
	Transmembrane	25 – 44	20	Helical; Potential
	Topological domain	45 – 74	30	Periplasmic Potential
	Transmembrane	75 – 94	20	Helical; Potential
	Topological domain	95 – 117	23	Cytoplasmic Potential
	Transmembrane	118 – 137	20	Helical; Potential
	Topological domain	138 – 167	30	Periplasmic Potential
	Transmembrane	168 – 187	20	Helical; Potential
	Topological domain	188 – 232	45	Cytoplasmic Potential
	Transmembrane	233 – 252	20	Helical; Potential
	Topological domain	253 – 283	31	Periplasmic Potential
	Transmembrane	284 – 303	20	Helical; Potential
	Topological domain	304 – 319	16	Cytoplasmic Potential
	Domain	75 – 305	231	ABC transmembrane type-1

Sequences

	Sequence		Length	Mass (Da)
	P0AGH8 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: 843F9E811DB62597	FASTA	319	34,121
	10 20 30 40 50 60 MAATKPAFNP PGKKGDIIFS VLVKLAALIV LLMLGGIIVS LIISSWPSIQ KFGLAFLWTK 70 80 90 100 110 120 EWDAPNDIYG ALVPIYGTLV TSFIALLIAV PVSFGIALFL TELAPGWLKR PLGIAIELLA 130 140 150 160 170 180 AIPSIVYGMW GLFIFAPLFA VYFQEPVGNI MSNIPIVGAL FSGPAFGIGI LAAGVILAIM 190 200 210 220 230 240 IIPYIAAVMR DVFEQTPVMM KESAYGIGCT TWEVIWRIVL PFTKNGVIGG IMLGLGRALG 250 260 270 280 290 300 ETMAVTFIIG NTYQLDSASL YMPGNSITSA LANEFAEAES GLHVAALMEL GLILFVITFI 310 VLAASKFMIM RLAKNEGAR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the genes involved in phosphate transport and regulation of the phosphate regulon in Escherichia coli." Amemura M., Makino K., Shinagawa H., Kobayashi A., Nakata A. J. Mol. Biol. 184:241-250(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Phosphate-specific transport system of Escherichia coli: nucleotide sequence and gene-polypeptide relationships." Surin B.P., Rosenberg H., Cox G.B. J. Bacteriol. 161:189-198(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R. Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Mutational analysis of the Escherichia coli phosphate-specific transport system, a member of the traffic ATPase (or ABC) family of membrane transporters. A role for proline residues in transmembrane helices." Webb D.C., Rosenberg H., Cox G.B. J. Biol. Chem. 267:24661-24668(1992) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS.
[7]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X02723 Genomic DNA. Translation: CAA26507.1. K01992 Genomic DNA. Translation: AAA24379.1. L10328 Genomic DNA. Translation: AAA62078.1. U00096 Genomic DNA. Translation: AAC76750.1. AP009048 Genomic DNA. Translation: BAE77561.1.
PIR	BVECPW. H65175.
RefSeq	NP_418183.1. NC_000913.2. YP_491702.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0AGH8.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b3727.
Protein family/group databases
TCDB	3.A.1.7.1. ATP-binding cassette (ABC) superfamily.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76750; AAC76750; b3727. BAE77561; BAE77561; BAE77561.
GeneID	12933146. 948238.
KEGG	ecj:Y75_p3441. eco:b3727.
PATRIC	32122951. VBIEscCol129921_3851.
Organism-specific databases
EchoBASE	EB0777.
EcoGene	EG10784. pstC.
Phylogenomic databases
eggNOG	COG0573.
HOGENOM	HOG000074139.
KO	K02037.
OMA	PIFGEYF.
ProtClustDB	PRK11275.
Enzyme and pathway databases
BioCyc	EcoCyc:PSTC-MONOMER. ECOL316407:JW3705-MONOMER.
Gene expression databases
Genevestigator	P0AGH8.
Family and domain databases
InterPro	IPR000515. BPD_transp. IPR011864. Phosphate_PstC. [Graphical view]
Pfam	PF00528. BPD_transp_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR02138. phosphate_pstC. 1 hit.
PROSITE	PS50928. ABC_TM1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSTC_ECOLI

Accession

Primary (citable) accession number: P0AGH8
Secondary accession number(s): P07653, Q2M845

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program