P0AGH3 (SAPB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptide transport system permease protein SapB

Gene names

Name:	sapB
Ordered Locus Names:	b1293, JW1286

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in a peptide intake transport system that plays a role in the resistance to antimicrobial peptides.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the binding-protein-dependent transport system permease family. OppBC subfamily. Contains 1 ABC transmembrane type-1 domain.

Ontologies

Keywords
Biological process	Peptide transport Protein transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptide transport Inferred from electronic annotation. Source: UniProtKB-KW protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Peptide transport system permease protein SapB		PRO_0000060158
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 29	21	Helical; Potential
	Topological domain	30 – 80	51	Periplasmic Potential
	Transmembrane	81 – 101	21	Helical; Potential
	Topological domain	102 – 112	11	Cytoplasmic Potential
	Transmembrane	113 – 133	21	Helical; Potential
	Topological domain	134 – 174	41	Periplasmic Potential
	Transmembrane	175 – 195	21	Helical; Potential
	Topological domain	196 – 248	53	Cytoplasmic Potential
	Transmembrane	249 – 269	21	Helical; Potential
	Topological domain	270 – 280	11	Periplasmic Potential
	Transmembrane	281 – 301	21	Helical; Potential
	Topological domain	302 – 321	20	Cytoplasmic Potential
	Domain	74 – 302	229	ABC transmembrane type-1

Sequences

	Sequence		Length	Mass (Da)
	P0AGH3 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: E50607A4A2C551F2	FASTA	321	36,038
	10 20 30 40 50 60 MIIFTLRRIL LLIVTLFLLT FVGFSLSYFT PHAPLQGASL WNAWVFWFNG LIHWDFGVSS 70 80 90 100 110 120 INGQPIAEQL KEVFPATMEL CILAFGFALI VGIPVGMIAG ITRHKWQDNL INAIALLGFS 130 140 150 160 170 180 IPVFWLALLL TLFCSLTLGW LPVSGRFDLL YEVKPITGFA LIDAWLSDSP WRDEMIMSAI 190 200 210 220 230 240 RHMILPVITL SVAPTTEVIR LMRISTIEVY DQNYVKAAAT RGLSRFTILR RHVLHNALPP 250 260 270 280 290 300 VIPRLGLQFS TMLTLAMITE MVFSWPGLGR WLINAIRQQD YAAISAGVMV CGSLVIIVNV 310 320 ISDILGAMAN PLKHKEWYAL R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Epstein W., Noelker E., Stumpe S., Tewes R., Schmid R., Bakker E.P. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / FRAG5.
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X97282 Genomic DNA. Translation: CAA65938.1. U00096 Genomic DNA. Translation: AAC74375.1. AP009048 Genomic DNA. Translation: BAA14854.1.
PIR	H64877.
RefSeq	NP_415809.1. NC_000913.2. YP_489561.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0AGH3.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b1293.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74375; AAC74375; b1293. BAA14854; BAA14854; BAA14854.
GeneID	12931142. 946191.
KEGG	ecj:Y75_p1268. eco:b1293.
PATRIC	32117856. VBIEscCol129921_1348.
Organism-specific databases
EchoBASE	EB4156.
EcoGene	EG20255. sapB.
Phylogenomic databases
eggNOG	COG4168.
HOGENOM	HOG000170242.
KO	K12369.
OMA	ATIELCI.
ProtClustDB	PRK15110.
Enzyme and pathway databases
BioCyc	EcoCyc:SAPB-MONOMER. ECOL316407:JW1286-MONOMER.
Gene expression databases
Genevestigator	P0AGH3.
Family and domain databases
InterPro	IPR000515. BPD_transp. [Graphical view]
Pfam	PF00528. BPD_transp_1. 1 hit. [Graphical view]
PROSITE	PS50928. ABC_TM1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAPB_ECOLI

Accession

Primary (citable) accession number: P0AGH3
Secondary accession number(s): P76838, Q47623

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program