D-xylose-proton symporter - Escherichia coli (strain K12)

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P0AGF4 (XYLE_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 58. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
D-xylose-proton symporter

Alternative name(s):
D-xylose transporter

Gene names

Name:	xylE
Ordered Locus Names:	b4031, JW3991

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Uptake of D-xylose across the boundary membrane with the concomitant transport of protons into the cell (symport system).
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Induction	By xylose.
Miscellaneous	E.coli has two D-xylose transport systems that accumulate sugar against a concentration gradient: the xylE system which utilizes the electrochemical gradient of protons and that is insensitive to cold osmotic shock and the xylF system that uses a high-energy phosphate compound and is sensitive to cold osmotic shock.
Sequence similarities	Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. [View classification]

Ontologies

Keywords
Biological process	Sugar transport Symport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	substrate-specific transmembrane transporter activity Inferred from electronic annotation. Source: InterPro symporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 491

491

D-xylose-proton symporter

PRO_0000050294

Regions

Topological domain

1 – 9

Cytoplasmic Potential

Transmembrane

10 – 30

Helical; Name=1; Potential

Topological domain

31 – 55

Periplasmic Potential

Transmembrane

56 – 76

Helical; Name=2; Potential

Topological domain

77 – 89

Cytoplasmic Potential

Transmembrane

90 – 110

Helical; Name=3; Potential

Topological domain

111 – 133

Periplasmic Potential

Transmembrane

134 – 154

Helical; Name=4; Potential

Topological domain

155 – 165

Cytoplasmic Potential

Transmembrane

166 – 186

Helical; Name=5; Potential

Topological domain

187 – 200

Periplasmic Potential

Transmembrane

201 – 221

Helical; Name=6; Potential

Topological domain

222 – 272

Cytoplasmic Potential

Transmembrane

273 – 293

Helical; Name=7; Potential

Topological domain

294 – 312

Periplasmic Potential

Transmembrane

313 – 333

Helical; Name=8; Potential

Topological domain

334 – 343

Cytoplasmic Potential

Transmembrane

344 – 364

Helical; Name=9; Potential

Topological domain

365 – 369

Periplasmic Potential

Transmembrane

370 – 390

Helical; Name=10; Potential

Topological domain

391 – 407

Cytoplasmic Potential

Transmembrane

408 – 428

Helical; Name=11; Potential

Topological domain

429 – 442

Periplasmic Potential

Transmembrane

443 – 463

Helical; Name=12; Potential

Topological domain

464 – 491

Cytoplasmic Potential

Experimental info

Sequence conflict

A → V in CAA29863. Ref.6

Sequences

Sequence

Length

Mass (Da)

Tools

P0AGF4 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: 2AF1AF9756C0B722
Show »

FASTA

491

53,608

        10         20         30         40         50         60 
MNTQYNSSYI FSITLVATLG GLLFGYDTAV ISGTVESLNT VFVAPQNLSE SAANSLLGFC 

        70         80         90        100        110        120 
VASALIGCII GGALGGYCSN RFGRRDSLKI AAVLFFISGV GSAWPELGFT SINPDNTVPV 

       130        140        150        160        170        180 
YLAGYVPEFV IYRIIGGIGV GLASMLSPMY IAELAPAHIR GKLVSFNQFA IIFGQLLVYC 

       190        200        210        220        230        240 
VNYFIARSGD ASWLNTDGWR YMFASECIPA LLFLMLLYTV PESPRWLMSR GKQEQAEGIL 

       250        260        270        280        290        300 
RKIMGNTLAT QAVQEIKHSL DHGRKTGGRL LMFGVGVIVI GVMLSIFQQF VGINVVLYYA 

       310        320        330        340        350        360 
PEVFKTLGAS TDIALLQTII VGVINLTFTV LAIMTVDKFG RKPLQIIGAL GMAIGMFSLG 

       370        380        390        400        410        420 
TAFYTQAPGI VALLSMLFYV AAFAMSWGPV CWVLLSEIFP NAIRGKALAI AVAAQWLANY 

       430        440        450        460        470        480 
FVSWTFPMMD KNSWLVAHFH NGFSYWIYGC MGVLAALFMW KFVPETKGKT LEELEALWEP 

       490 
ETKKTQQTAT L

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The cloning and DNA sequence of the gene xylE for xylose-proton symport in Escherichia coli K12." Davis E.O., Henderson P.J.F. J. Biol. Chem. 262:13928-13932(1987) [PubMed: 2820984] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Mammalian and bacterial sugar transport proteins are homologous." Maiden M.C.J., Davis E.O., Baldwin S.A., Moore D.C.M., Henderson P.J.F. Nature 325:641-643(1987) [PubMed: 3543693] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed: 8265357] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"3' end of the malEFG operon in E.coli: localization of the transcription termination site." Francoz E., Dassa E. Nucleic Acids Res. 16:4097-4109(1988) [PubMed: 2836810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-192. Strain: K12.
[7]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J02812 Genomic DNA. Translation: AAA79016.1. U00006 Genomic DNA. Translation: AAC43125.1. U00096 Genomic DNA. Translation: AAC77001.1. AP009048 Genomic DNA. Translation: BAE78033.1. X06663 Genomic DNA. Translation: CAA29863.1.
PIR	A26430.
RefSeq	NP_418455.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0AGF4.
ModBase	Search...
Protein family/group databases
TCDB	2.A.1.1.3. major facilitator superfamily (MFS).
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002159; EBESCP00000002159; EBESCG00000001768. EBESCT00000014650; EBESCP00000013941; EBESCG00000013711.
GeneID	948529.
GenomeReviews	Gene locus JW3991 in contig AP009048_GR. Gene locus b4031 in contig U00096_GR.
KEGG	ecj:JW3991. eco:b4031.
PATRIC	32123595. VBIEscCol129921_4146.
Organism-specific databases
EchoBASE	EB1069.
EcoGene	EG11076. xylE.
Phylogenomic databases
eggNOG	COG0477.
GeneTree	EBGT00050000008817.
HOGENOM	HBG744444.
OMA	SLEHGRK.
PhylomeDB	P0AGF4.
ProtClustDB	PRK10077.
Enzyme and pathway databases
BioCyc	EcoCyc:XYLE-MONOMER.
Gene expression databases
Genevestigator	P0AGF4.
Family and domain databases
InterPro	IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. IPR005828. Sub_transporter. IPR003663. Sugar/inositol_transpt. IPR005829. Sugar_transporter_CS. [Graphical view]
KO	K08138.
Pfam	PF00083. Sugar_tr. 1 hit. [Graphical view]
PRINTS	PR00171. SUGRTRNSPORT.
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMs	TIGR00879. SP. 1 hit.
PROSITE	PS50850. MFS. 1 hit. PS00216. SUGAR_TRANSPORT_1. 1 hit. PS00217. SUGAR_TRANSPORT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYLE_ECOLI

Accession

Primary (citable) accession number: P0AGF4
Secondary accession number(s): P09098, Q2M6S3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents