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Protein

Sulfur acceptor protein CsdE

Gene

csdE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Stimulates the cysteine desulfurase activity of CsdA. Contains a cysteine residue (Cys-61) that acts to accept sulfur liberated via the desulfurase activity of CsdA. May be able to transfer sulfur to TcdA/CsdL. Seems to support the function of TcdA in the generation of cyclic threonylcarbamoyladenosine at position 37 (ct6A37) in tRNAs that read codons beginning with adenine. Does not appear to participate in Fe/S biogenesis.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei61Cysteine persulfide intermediate1

GO - Biological processi

  • iron-sulfur cluster assembly Source: EcoCyc

Enzyme and pathway databases

BioCyciEcoCyc:G7455-MONOMER
MetaCyc:G7455-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfur acceptor protein CsdE
Gene namesi
Name:csdE
Synonyms:ygdK
Ordered Locus Names:b2811, JW2782
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13083 csdE

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene display a high decrease in the level of ct6A modification in tRNAs, and show the t6A modification instead. They also show no defects in motility, fitness or antibiotic sensitivity, in contrast to csdA mutants.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi61C → S: Unable to exert a stimulatory effect on the cysteine desulfurase activity of CsdA and to accept sulfur. Retains the ability to interact with CsdA. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002021361 – 147Sulfur acceptor protein CsdEAdd BLAST147

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei61Cysteine persulfide1 Publication1

Proteomic databases

PaxDbiP0AGF2
PRIDEiP0AGF2

Interactioni

Subunit structurei

Homodimer. Forms a heterodimer with CsdA. Interacts with CsdA and with TcdA/CsdL.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4261123, 398 interactors
DIPiDIP-12143N
IntActiP0AGF2, 5 interactors
STRINGi316385.ECDH10B_2981

Structurei

Secondary structure

1147
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni12 – 14Combined sources3
Helixi17 – 24Combined sources8
Helixi30 – 41Combined sources12
Helixi49 – 52Combined sources4
Beta strandi55 – 57Combined sources3
Beta strandi60 – 63Combined sources4
Beta strandi65 – 71Combined sources7
Beta strandi73 – 75Combined sources3
Beta strandi77 – 85Combined sources9
Helixi86 – 99Combined sources14
Helixi104 – 109Combined sources6
Helixi114 – 119Combined sources6
Beta strandi121 – 125Combined sources5
Helixi127 – 145Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NI7NMR-A1-147[»]
5EEPX-ray2.40A1-147[»]
5FT8X-ray2.50B/D/F/H/J/L/N/P1-147[»]
ProteinModelPortaliP0AGF2
SMRiP0AGF2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AGF2

Family & Domainsi

Sequence similaritiesi

Belongs to the SufE family.Curated

Phylogenomic databases

eggNOGiENOG4105P9C Bacteria
COG2166 LUCA
HOGENOMiHOG000285041
InParanoidiP0AGF2
KOiK02426
OMAiHYFLADS
PhylomeDBiP0AGF2

Family and domain databases

InterProiView protein in InterPro
IPR017763 Cysteine_desulfurase_CsdE
IPR003808 Fe-S_metab-assoc_dom
PANTHERiPTHR43597 PTHR43597, 1 hit
PfamiView protein in Pfam
PF02657 SufE, 1 hit
TIGRFAMsiTIGR03391 FeS_syn_CsdE, 1 hit

Sequencei

Sequence statusi: Complete.

P0AGF2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNPQFAGHP FGTTVTAETL RNTFAPLTQW EDKYRQLIML GKQLPALPDE
60 70 80 90 100
LKAQAKEIAG CENRVWLGYT VAENGKMHFF GDSEGRIVRG LLAVLLTAVE
110 120 130 140
GKTAAELQAQ SPLALFDELG LRAQLSASRS QGLNALSEAI IAATKQV
Length:147
Mass (Da):15,940
Last modified:December 20, 2005 - v1
Checksum:iEECAB64427D42C41
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29581 Genomic DNA Translation: AAB40461.1
U00096 Genomic DNA Translation: AAC75853.1
AP009048 Genomic DNA Translation: BAE76883.1
PIRiG65063
RefSeqiNP_417291.1, NC_000913.3
WP_000184261.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75853; AAC75853; b2811
BAE76883; BAE76883; BAE76883
GeneIDi947274
KEGGiecj:JW2782
eco:b2811
PATRICifig|1411691.4.peg.3922

Similar proteinsi

Entry informationi

Entry nameiCSDE_ECOLI
AccessioniPrimary (citable) accession number: P0AGF2
Secondary accession number(s): Q2MA23, Q46926
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: April 25, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health