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Protein

Sulfur acceptor protein CsdE

Gene

csdE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Stimulates the cysteine desulfurase activity of CsdA. Contains a cysteine residue (Cys-61) that acts to accept sulfur liberated via the desulfurase activity of CsdA. May be able to transfer sulfur to TcdA/CsdL. Seems to support the function of TcdA in the generation of cyclic threonylcarbamoyladenosine at position 37 (ct6A37) in tRNAs that read codons beginning with adenine. Does not appear to participate in Fe/S biogenesis.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei61Cysteine persulfide intermediate1

GO - Biological processi

  • iron-sulfur cluster assembly Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:G7455-MONOMER.
ECOL316407:JW2782-MONOMER.
MetaCyc:G7455-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfur acceptor protein CsdE
Gene namesi
Name:csdE
Synonyms:ygdK
Ordered Locus Names:b2811, JW2782
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13083. csdE.

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene display a high decrease in the level of ct6A modification in tRNAs, and show the t6A modification instead. They also show no defects in motility, fitness or antibiotic sensitivity, in contrast to csdA mutants.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi61C → S: Unable to exert a stimulatory effect on the cysteine desulfurase activity of CsdA and to accept sulfur. Retains the ability to interact with CsdA. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002021361 – 147Sulfur acceptor protein CsdEAdd BLAST147

Proteomic databases

PaxDbiP0AGF2.
PRIDEiP0AGF2.

Interactioni

Subunit structurei

Homodimer. Forms a heterodimer with CsdA. Interacts with CsdA and with TcdA/CsdL.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
csdAQ469258EBI-1130454,EBI-545660
tcdAQ469273EBI-1130454,EBI-1130463

Protein-protein interaction databases

BioGridi4261123. 398 interactors.
DIPiDIP-12143N.
IntActiP0AGF2. 5 interactors.
STRINGi511145.b2811.

Structurei

Secondary structure

1147
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni12 – 14Combined sources3
Helixi17 – 24Combined sources8
Helixi30 – 41Combined sources12
Helixi49 – 52Combined sources4
Beta strandi55 – 57Combined sources3
Beta strandi60 – 63Combined sources4
Beta strandi65 – 71Combined sources7
Beta strandi73 – 75Combined sources3
Beta strandi77 – 85Combined sources9
Helixi86 – 99Combined sources14
Helixi104 – 109Combined sources6
Helixi114 – 119Combined sources6
Beta strandi121 – 125Combined sources5
Helixi127 – 145Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NI7NMR-A1-147[»]
5EEPX-ray2.40A1-147[»]
ProteinModelPortaliP0AGF2.
SMRiP0AGF2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AGF2.

Family & Domainsi

Sequence similaritiesi

Belongs to the SufE family.Curated

Phylogenomic databases

eggNOGiENOG4105P9C. Bacteria.
COG2166. LUCA.
HOGENOMiHOG000285041.
InParanoidiP0AGF2.
KOiK02426.
OMAiCESKVWL.
PhylomeDBiP0AGF2.

Family and domain databases

InterProiIPR017763. Cysteine_desulfurase_CsdE.
IPR003808. Fe-S_metab-assoc_dom.
[Graphical view]
PfamiPF02657. SufE. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03391. FeS_syn_CsdE. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AGF2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNPQFAGHP FGTTVTAETL RNTFAPLTQW EDKYRQLIML GKQLPALPDE
60 70 80 90 100
LKAQAKEIAG CENRVWLGYT VAENGKMHFF GDSEGRIVRG LLAVLLTAVE
110 120 130 140
GKTAAELQAQ SPLALFDELG LRAQLSASRS QGLNALSEAI IAATKQV
Length:147
Mass (Da):15,940
Last modified:December 20, 2005 - v1
Checksum:iEECAB64427D42C41
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29581 Genomic DNA. Translation: AAB40461.1.
U00096 Genomic DNA. Translation: AAC75853.1.
AP009048 Genomic DNA. Translation: BAE76883.1.
PIRiG65063.
RefSeqiNP_417291.1. NC_000913.3.
WP_000184261.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75853; AAC75853; b2811.
BAE76883; BAE76883; BAE76883.
GeneIDi947274.
KEGGiecj:JW2782.
eco:b2811.
PATRICi32121038. VBIEscCol129921_2911.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29581 Genomic DNA. Translation: AAB40461.1.
U00096 Genomic DNA. Translation: AAC75853.1.
AP009048 Genomic DNA. Translation: BAE76883.1.
PIRiG65063.
RefSeqiNP_417291.1. NC_000913.3.
WP_000184261.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NI7NMR-A1-147[»]
5EEPX-ray2.40A1-147[»]
ProteinModelPortaliP0AGF2.
SMRiP0AGF2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261123. 398 interactors.
DIPiDIP-12143N.
IntActiP0AGF2. 5 interactors.
STRINGi511145.b2811.

Proteomic databases

PaxDbiP0AGF2.
PRIDEiP0AGF2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75853; AAC75853; b2811.
BAE76883; BAE76883; BAE76883.
GeneIDi947274.
KEGGiecj:JW2782.
eco:b2811.
PATRICi32121038. VBIEscCol129921_2911.

Organism-specific databases

EchoBASEiEB2892.
EcoGeneiEG13083. csdE.

Phylogenomic databases

eggNOGiENOG4105P9C. Bacteria.
COG2166. LUCA.
HOGENOMiHOG000285041.
InParanoidiP0AGF2.
KOiK02426.
OMAiCESKVWL.
PhylomeDBiP0AGF2.

Enzyme and pathway databases

BioCyciEcoCyc:G7455-MONOMER.
ECOL316407:JW2782-MONOMER.
MetaCyc:G7455-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AGF2.
PROiP0AGF2.

Family and domain databases

InterProiIPR017763. Cysteine_desulfurase_CsdE.
IPR003808. Fe-S_metab-assoc_dom.
[Graphical view]
PfamiPF02657. SufE. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03391. FeS_syn_CsdE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCSDE_ECOLI
AccessioniPrimary (citable) accession number: P0AGF2
Secondary accession number(s): Q2MA23, Q46926
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.