P0AGF1 (SUCD_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Succinyl-CoA ligase [ADP-forming] subunit alpha
EC=6.2.1.5

Alternative name(s):
Succinyl-CoA synthetase subunit alpha
Short name=SCS-alpha

Gene names

Name:	sucD
Ordered Locus Names:	Z0883, ECs0754

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + succinate + CoA = ADP + phosphate + succinyl-CoA.
Subunit structure	Heterotetramer of two alpha and two beta subunits By similarity.
Sequence similarities	Belongs to the succinate/malate CoA ligase alpha subunit family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP citrate synthase activity Inferred from electronic annotation. Source: InterPro succinate-CoA ligase (ADP-forming) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 289	288	Succinyl-CoA ligase [ADP-forming] subunit alpha		PRO_0000102792
Sites
	Active site	247	1	Tele-phosphohistidine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0AGF1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 8AC27AD828BC51B3	FASTA	289	29,777
	10 20 30 40 50 60 MSILIDKNTK VICQGFTGSQ GTFHSEQAIA YGTKMVGGVT PGKGGTTHLG LPVFNTVREA 70 80 90 100 110 120 VAATGATASV IYVPAPFCKD SILEAIDAGI KLIITITEGI PTLDMLTVKV KLDEAGVRMI 130 140 150 160 170 180 GPNCPGVITP GECKIGIQPG HIHKPGKVGI VSRSGTLTYE AVKQTTDYGF GQSTCVGIGG 190 200 210 220 230 240 DPIPGSNFID ILEMFEKDPQ TEAIVMIGEI GGSAEEEAAA YIKEHVTKPV VGYIAGVTAP 250 260 270 280 KGKRMGHAGA IIAGGKGTAD EKFAALEAAG VKTVRSLADI GEALKTVLK

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG55053.1. BA000007 Genomic DNA. Translation: BAB34177.1.
PIR	A85574. B90723.
RefSeq	NP_286445.1. NC_002655.2. NP_308781.1. NC_002695.1.
3D structure databases
ProteinModelPortal	P0AGF1.
SMR	P0AGF1. Positions 2-288.
ModBase	Search...
Proteomic databases
PRIDE	P0AGF1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000024600; EBESCP00000023493; EBESCG00000023654. EBESCT00000058382; EBESCP00000056210; EBESCG00000057430.
GeneID	917489. 957836.
GenomeReviews	Gene locus Z0883 in contig AE005174_GR. Gene locus ECs0754 in contig BA000007_GR.
KEGG	ece:Z0883. ecs:ECs0754.
PATRIC	18350530. VBIEscCol44059_0776.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000010106.
HOGENOM	HBG615372.
OMA	DETTEAI.
ProtClustDB	PRK05678.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS0754-MONOMER.
Family and domain databases
InterPro	IPR017440. Cit_synth/succinyl-CoA_lig_AS. IPR003781. CoA-bd. IPR005810. CoA_lig_alpha. IPR005811. CoA_ligase. IPR016040. NAD(P)-bd_dom. IPR016102. Succinyl-CoA_synth-like. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:3.40.50.261. Succinyl-CoA_synth-like. 1 hit.
KO	K01902.
Pfam	PF02629. CoA_binding. 1 hit. PF00549. Ligase_CoA. 1 hit. [Graphical view]
PIRSF	PIRSF001553. SucCS_alpha. 1 hit.
PRINTS	PR01798. SCOASYNTHASE.
SMART	SM00881. CoA_binding. 1 hit. [Graphical view]
SUPFAM	SSF52210. CoA_ligase. 1 hit.
TIGRFAMs	TIGR01019. SucCoAalpha. 1 hit.
PROSITE	PS01216. SUCCINYL_COA_LIG_1. 1 hit. PS00399. SUCCINYL_COA_LIG_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SUCD_ECO57

Accession

Primary (citable) accession number: P0AGF1
Secondary accession number(s): P07459

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program