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Protein

Serine/threonine transporter SstT

Gene

sstT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the import of serine and threonine into the cell, with the concomitant import of sodium (symport system).2 Publications

Enzyme regulationi

Activated by valinomycin and inhibited by monensin.1 Publication

Kineticsi

  1. KM=0.82 µM for serine1 Publication
  1. Vmax=0.37 µmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 7.0.1 Publication

Temperature dependencei

Optimum temperature is 40 degrees Celsius.1 Publication

GO - Molecular functioni

  • neutral amino acid:sodium symporter activity Source: EcoliWiki
  • neutral amino acid transmembrane transporter activity Source: EcoliWiki

GO - Biological processi

  • amino acid transport Source: EcoliWiki
  • serine transport Source: EcoliWiki
  • threonine transport Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Symport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:YGJU-MONOMER.
ECOL316407:JW3060-MONOMER.
MetaCyc:YGJU-MONOMER.

Protein family/group databases

TCDBi2.A.23.4.1. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine transporter SstT
Alternative name(s):
Na(+)/serine-threonine symporter
Gene namesi
Name:sstT
Synonyms:ygjU
Ordered Locus Names:b3089, JW3060
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12732. sstT.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 1514CytoplasmicSequence analysisAdd
BLAST
Transmembranei16 – 3621HelicalSequence analysisAdd
BLAST
Topological domaini37 – 459PeriplasmicSequence analysis
Transmembranei46 – 6621HelicalSequence analysisAdd
BLAST
Topological domaini67 – 8317CytoplasmicSequence analysisAdd
BLAST
Transmembranei84 – 10421HelicalSequence analysisAdd
BLAST
Topological domaini105 – 14238PeriplasmicSequence analysisAdd
BLAST
Transmembranei143 – 16321HelicalSequence analysisAdd
BLAST
Topological domaini164 – 17916CytoplasmicSequence analysisAdd
BLAST
Transmembranei180 – 20021HelicalSequence analysisAdd
BLAST
Topological domaini201 – 21717PeriplasmicSequence analysisAdd
BLAST
Transmembranei218 – 23821HelicalSequence analysisAdd
BLAST
Topological domaini239 – 29961CytoplasmicSequence analysisAdd
BLAST
Transmembranei300 – 32021HelicalSequence analysisAdd
BLAST
Topological domaini321 – 33111PeriplasmicSequence analysisAdd
BLAST
Transmembranei332 – 35221HelicalSequence analysisAdd
BLAST
Topological domaini353 – 41462CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 414413Serine/threonine transporter SstTPRO_0000202118Add
BLAST

Proteomic databases

EPDiP0AGE4.
PaxDbiP0AGE4.

Expressioni

Inductioni

Repressed by tryptophan.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4260951. 7 interactions.
STRINGi511145.b3089.

Structurei

3D structure databases

ProteinModelPortaliP0AGE4.
SMRiP0AGE4. Positions 18-403.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107QV7. Bacteria.
COG3633. LUCA.
HOGENOMiHOG000218252.
InParanoidiP0AGE4.
KOiK07862.
OMAiMYQRIGL.
PhylomeDBiP0AGE4.

Family and domain databases

Gene3Di1.10.3860.10. 1 hit.
HAMAPiMF_01582. Ser_Thr_transp_SstT. 1 hit.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR023025. Ser_Thr_transp_SstT.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 1 hit.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AGE4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTQRSPGLF RRLAHGSLVK QILVGLVLGI LLAWISKPAA EAVGLLGTLF
60 70 80 90 100
VGALKAVAPI LVLMLVMASI ANHQHGQKTN IRPILFLYLL GTFSAALAAV
110 120 130 140 150
VFSFAFPSTL HLSSSAGDIS PPSGIVEVMR GLVMSMVSNP IDALLKGNYI
160 170 180 190 200
GILVWAIGLG FALRHGNETT KNLVNDMSNA VTFMVKLVIR FAPIGIFGLV
210 220 230 240 250
SSTLATTGFS TLWGYAQLLV VLVGCMLLVA LVVNPLLVWW KIRRNPFPLV
260 270 280 290 300
LLCLRESGVY AFFTRSSAAN IPVNMALCEK LNLDRDTYSV SIPLGATINM
310 320 330 340 350
AGAAITITVL TLAAVNTLGI PVDLPTALLL SVVASLCACG ASGVAGGSLL
360 370 380 390 400
LIPLACNMFG ISNDIAMQVV AVGFIIGVLQ DSCETALNSS TDVLFTAAAC
410
QAEDDRLANS ALRN
Length:414
Mass (Da):43,478
Last modified:December 20, 2005 - v1
Checksum:i47713C1DB77B1E38
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57891.1.
U00096 Genomic DNA. Translation: AAC76124.1.
AP009048 Genomic DNA. Translation: BAE77139.1.
D13328 Genomic DNA. No translation available.
PIRiF65097.
RefSeqiNP_417560.1. NC_000913.3.
WP_000211655.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76124; AAC76124; b3089.
BAE77139; BAE77139; BAE77139.
GeneIDi947605.
KEGGiecj:JW3060.
eco:b3089.
PATRICi32121596. VBIEscCol129921_3184.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57891.1.
U00096 Genomic DNA. Translation: AAC76124.1.
AP009048 Genomic DNA. Translation: BAE77139.1.
D13328 Genomic DNA. No translation available.
PIRiF65097.
RefSeqiNP_417560.1. NC_000913.3.
WP_000211655.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AGE4.
SMRiP0AGE4. Positions 18-403.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260951. 7 interactions.
STRINGi511145.b3089.

Protein family/group databases

TCDBi2.A.23.4.1. the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family.

Proteomic databases

EPDiP0AGE4.
PaxDbiP0AGE4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76124; AAC76124; b3089.
BAE77139; BAE77139; BAE77139.
GeneIDi947605.
KEGGiecj:JW3060.
eco:b3089.
PATRICi32121596. VBIEscCol129921_3184.

Organism-specific databases

EchoBASEiEB2590.
EcoGeneiEG12732. sstT.

Phylogenomic databases

eggNOGiENOG4107QV7. Bacteria.
COG3633. LUCA.
HOGENOMiHOG000218252.
InParanoidiP0AGE4.
KOiK07862.
OMAiMYQRIGL.
PhylomeDBiP0AGE4.

Enzyme and pathway databases

BioCyciEcoCyc:YGJU-MONOMER.
ECOL316407:JW3060-MONOMER.
MetaCyc:YGJU-MONOMER.

Miscellaneous databases

PROiP0AGE4.

Family and domain databases

Gene3Di1.10.3860.10. 1 hit.
HAMAPiMF_01582. Ser_Thr_transp_SstT. 1 hit.
InterProiIPR001991. Na-dicarboxylate_symporter.
IPR023025. Ser_Thr_transp_SstT.
[Graphical view]
PANTHERiPTHR11958. PTHR11958. 1 hit.
PfamiPF00375. SDF. 1 hit.
[Graphical view]
PRINTSiPR00173. EDTRNSPORT.
SUPFAMiSSF118215. SSF118215. 1 hit.
PROSITEiPS00713. NA_DICARBOXYL_SYMP_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSSTT_ECOLI
AccessioniPrimary (citable) accession number: P0AGE4
Secondary accession number(s): P42602, Q2M9B7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: September 7, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.