Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Hexose-6-phosphate:phosphate antiporter

Gene

uhpT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the exchange of external hexose 6-phosphate and internal inorganic phosphate. Can transport glucose-6-phosphate, fructose-6-phosphate and mannose-6-phosphate. Also catalyzes the neutral exchange of internal and external phosphate.6 Publications

Enzyme regulationi

Reaction is unaffected by the ionophores valinomycin, valinomycin plus nigericin, and carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP).1 Publication

GO - Molecular functioni

  • hexose-phosphate:inorganic phosphate antiporter activity Source: EcoCyc

GO - Biological processi

  • carbohydrate transport Source: UniProtKB-KW
  • glucose-6-phosphate transport Source: EcoCyc
  • hexose phosphate transport Source: EcoCyc
  • phosphate ion transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Phosphate transport, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:UHPT-MONOMER.
ECOL316407:JW3641-MONOMER.
MetaCyc:UHPT-MONOMER.

Protein family/group databases

TCDBi2.A.1.4.1. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Hexose-6-phosphate:phosphate antiporterCurated
Gene namesi
Name:uhpT
Ordered Locus Names:b3666, JW3641
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11054. uhpT.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2424Cytoplasmic1 PublicationAdd
BLAST
Transmembranei25 – 4521HelicalSequence analysisAdd
BLAST
Topological domaini46 – 6015Periplasmic1 PublicationAdd
BLAST
Transmembranei61 – 8121HelicalSequence analysisAdd
BLAST
Topological domaini82 – 9615Cytoplasmic1 PublicationAdd
BLAST
Transmembranei97 – 11721HelicalSequence analysisAdd
BLAST
Topological domaini118 – 1203Periplasmic1 Publication
Transmembranei121 – 14121HelicalSequence analysisAdd
BLAST
Topological domaini142 – 15918Cytoplasmic2 PublicationsAdd
BLAST
Transmembranei160 – 18021HelicalSequence analysisAdd
BLAST
Topological domaini181 – 1899Periplasmic1 Publication
Transmembranei190 – 21021HelicalSequence analysisAdd
BLAST
Topological domaini211 – 25949Cytoplasmic1 PublicationAdd
BLAST
Transmembranei260 – 28021HelicalSequence analysisAdd
BLAST
Topological domaini281 – 29717Periplasmic1 PublicationAdd
BLAST
Transmembranei298 – 31821HelicalSequence analysisAdd
BLAST
Topological domaini319 – 3268Cytoplasmic1 Publication
Transmembranei327 – 34721HelicalSequence analysisAdd
BLAST
Topological domaini348 – 35710Periplasmic1 Publication
Transmembranei358 – 37821HelicalSequence analysisAdd
BLAST
Topological domaini379 – 39113Cytoplasmic1 PublicationAdd
BLAST
Transmembranei392 – 41120HelicalSequence analysisAdd
BLAST
Topological domaini412 – 42514Periplasmic1 PublicationAdd
BLAST
Transmembranei426 – 44621HelicalSequence analysisAdd
BLAST
Topological domaini447 – 46317Cytoplasmic1 Publication1 PublicationAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: GO_Central
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi108 – 1081C → S: No change in activity. 1 Publication
Mutagenesisi143 – 1431C → S: 30% of wild-type sugar phosphate transport activity. 1 Publication
Mutagenesisi265 – 2651C → S: No change in activity. 1 Publication
Mutagenesisi331 – 3311C → S: No change in activity. 1 Publication
Mutagenesisi436 – 4361C → S: No change in activity. 1 Publication
Mutagenesisi438 – 4381C → S: No change in activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 463463Hexose-6-phosphate:phosphate antiporterPRO_0000199883Add
BLAST

Proteomic databases

PaxDbiP0AGC0.

Expressioni

Inductioni

Expression is induced by external glucose-6-phosphate through the UhpABC signaling cascade.4 Publications

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4262581. 5 interactions.
STRINGi511145.b3666.

Structurei

3D structure databases

ProteinModelPortaliP0AGC0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CXY. Bacteria.
COG2271. LUCA.
HOGENOMiHOG000274728.
InParanoidiP0AGC0.
KOiK07784.
OMAiWANIHAF.
OrthoDBiEOG6M9DW7.
PhylomeDBiP0AGC0.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR021159. Sugar-P_transporter_CS.
IPR000849. Sugar_P_transporter.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
PIRSFiPIRSF002808. Hexose_phosphate_transp. 1 hit.
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00881. 2A0104. 1 hit.
PROSITEiPS00942. GLPT. 1 hit.
PS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AGC0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAFLNQVRK PTLDLPLEVR RKMWFKPFMQ SYLVVFIGYL TMYLIRKNFN
60 70 80 90 100
IAQNDMISTY GLSMTQLGMI GLGFSITYGV GKTLVSYYAD GKNTKQFLPF
110 120 130 140 150
MLILSAICML GFSASMGSGS VSLFLMIAFY ALSGFFQSTG GSCSYSTITK
160 170 180 190 200
WTPRRKRGTF LGFWNISHNL GGAGAAGVAL FGANYLFDGH VIGMFIFPSI
210 220 230 240 250
IALIVGFIGL RYGSDSPESY GLGKAEELFG EEISEEDKET ESTDMTKWQI
260 270 280 290 300
FVEYVLKNKV IWLLCFANIF LYVVRIGIDQ WSTVYAFQEL KLSKAVAIQG
310 320 330 340 350
FTLFEAGALV GTLLWGWLSD LANGRRGLVA CIALALIIAT LGVYQHASNE
360 370 380 390 400
YIYLASLFAL GFLVFGPQLL IGVAAVGFVP KKAIGAADGI KGTFAYLIGD
410 420 430 440 450
SFAKLGLGMI ADGTPVFGLT GWAGTFAALD IAAIGCICLM AIVAVMEERK
460
IRREKKIQQL TVA
Length:463
Mass (Da):50,607
Last modified:December 20, 2005 - v1
Checksum:i1BE23CEFF05311B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17102 Genomic DNA. Translation: AAA24723.1.
M89479 Genomic DNA. Translation: AAA24727.1.
L10328 Genomic DNA. Translation: AAA62018.1.
U00096 Genomic DNA. Translation: AAC76689.1.
AP009048 Genomic DNA. Translation: BAE77627.1.
PIRiA30395. MMECHP.
RefSeqiNP_418122.1. NC_000913.3.
WP_000879194.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76689; AAC76689; b3666.
BAE77627; BAE77627; BAE77627.
GeneIDi948201.
KEGGiecj:JW3641.
eco:b3666.
PATRICi32122825. VBIEscCol129921_3788.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17102 Genomic DNA. Translation: AAA24723.1.
M89479 Genomic DNA. Translation: AAA24727.1.
L10328 Genomic DNA. Translation: AAA62018.1.
U00096 Genomic DNA. Translation: AAC76689.1.
AP009048 Genomic DNA. Translation: BAE77627.1.
PIRiA30395. MMECHP.
RefSeqiNP_418122.1. NC_000913.3.
WP_000879194.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AGC0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262581. 5 interactions.
STRINGi511145.b3666.

Protein family/group databases

TCDBi2.A.1.4.1. the major facilitator superfamily (mfs).

Proteomic databases

PaxDbiP0AGC0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76689; AAC76689; b3666.
BAE77627; BAE77627; BAE77627.
GeneIDi948201.
KEGGiecj:JW3641.
eco:b3666.
PATRICi32122825. VBIEscCol129921_3788.

Organism-specific databases

EchoBASEiEB1047.
EcoGeneiEG11054. uhpT.

Phylogenomic databases

eggNOGiENOG4105CXY. Bacteria.
COG2271. LUCA.
HOGENOMiHOG000274728.
InParanoidiP0AGC0.
KOiK07784.
OMAiWANIHAF.
OrthoDBiEOG6M9DW7.
PhylomeDBiP0AGC0.

Enzyme and pathway databases

BioCyciEcoCyc:UHPT-MONOMER.
ECOL316407:JW3641-MONOMER.
MetaCyc:UHPT-MONOMER.

Miscellaneous databases

PROiP0AGC0.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR021159. Sugar-P_transporter_CS.
IPR000849. Sugar_P_transporter.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
PIRSFiPIRSF002808. Hexose_phosphate_transp. 1 hit.
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00881. 2A0104. 1 hit.
PROSITEiPS00942. GLPT. 1 hit.
PS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the uhp region of Escherichia coli."
    Friedrich M.J., Kadner R.J.
    J. Bacteriol. 169:3556-3563(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Structure and function of the uhp genes for the sugar phosphate transport system in Escherichia coli and Salmonella typhimurium."
    Island M.D., Wei B.-Y., Kadner R.J.
    J. Bacteriol. 174:2754-2762(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
    Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
    Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "A hexose-phosphate transport system in Escherichia coli."
    Winkler H.H.
    Biochim. Biophys. Acta 117:231-240(1966) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Compartmentation in the induction of the hexose-6-phosphate transport system of Escherichia coli."
    Winkler H.H.
    J. Bacteriol. 101:470-475(1970) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  8. "Reconstitution of sugar phosphate transport systems of Escherichia coli."
    Ambudkar S.V., Larson T.J., Maloney P.C.
    J. Biol. Chem. 261:9083-9086(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: K12.
  9. "Identification of uhp polypeptides and evidence for their role in exogenous induction of the sugar phosphate transport system of Escherichia coli K-12."
    Weston L.A., Kadner R.J.
    J. Bacteriol. 169:3546-3555(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  10. "The mechanism of glucose 6-phosphate transport by Escherichia coli."
    Sonna L.A., Ambudkar S.V., Maloney P.C.
    J. Biol. Chem. 263:6625-6630(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS AN HEXOSE-6-PHOSPHATE:PHOSPHATE ANTIPORTER, ENZYME REGULATION.
  11. "Topology of the Escherichia coli uhpT sugar-phosphate transporter analyzed by using TnphoA fusions."
    Lloyd A.D., Kadner R.J.
    J. Bacteriol. 172:1688-1693(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY, SUBCELLULAR LOCATION.
    Strain: K12.
  12. "UhpT, the sugar phosphate antiporter of Escherichia coli, functions as a monomer."
    Ambudkar S.V., Anantharam V., Maloney P.C.
    J. Biol. Chem. 265:12287-12292(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
    Strain: K12.
  13. "Identification of a residue in the translocation pathway of a membrane carrier."
    Yan R.T., Maloney P.C.
    Cell 75:37-44(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, MUTAGENESIS OF CYS-108; CYS-143; CYS-265; CYS-331; CYS-436 AND CYS-438.
  14. "Protein phosphorylation affects binding of the Escherichia coli transcription activator UhpA to the uhpT promoter."
    Dahl J.L., Wei B.Y., Kadner R.J.
    J. Biol. Chem. 272:1910-1919(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  15. "Glucose-6-phosphate-dependent phosphoryl flow through the Uhp two-component regulatory system."
    Verhamme D.T., Arents J.C., Postma P.W., Crielaard W., Hellingwerf K.J.
    Microbiology 147:3345-3352(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  16. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiUHPT_ECOLI
AccessioniPrimary (citable) accession number: P0AGC0
Secondary accession number(s): P13408, P76727, Q2M7X9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: May 11, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.