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Protein

Hexose-6-phosphate:phosphate antiporter

Gene

uhpT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the exchange of external hexose 6-phosphate and internal inorganic phosphate. Can transport glucose-6-phosphate, fructose-6-phosphate and mannose-6-phosphate. Also catalyzes the neutral exchange of internal and external phosphate.6 Publications

Enzyme regulationi

Reaction is unaffected by the ionophores valinomycin, valinomycin plus nigericin, and carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP).1 Publication

GO - Molecular functioni

  • hexose-phosphate:inorganic phosphate antiporter activity Source: EcoCyc

GO - Biological processi

  • carbohydrate transport Source: UniProtKB-KW
  • glucose-6-phosphate transport Source: EcoCyc
  • hexose phosphate transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Phosphate transport, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:UHPT-MONOMER.
ECOL316407:JW3641-MONOMER.
MetaCyc:UHPT-MONOMER.

Protein family/group databases

TCDBi2.A.1.4.1. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Hexose-6-phosphate:phosphate antiporterCurated
Gene namesi
Name:uhpT
Ordered Locus Names:b3666, JW3641
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11054. uhpT.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 24Cytoplasmic1 PublicationAdd BLAST24
Transmembranei25 – 45HelicalSequence analysisAdd BLAST21
Topological domaini46 – 60Periplasmic1 PublicationAdd BLAST15
Transmembranei61 – 81HelicalSequence analysisAdd BLAST21
Topological domaini82 – 96Cytoplasmic1 PublicationAdd BLAST15
Transmembranei97 – 117HelicalSequence analysisAdd BLAST21
Topological domaini118 – 120Periplasmic1 Publication3
Transmembranei121 – 141HelicalSequence analysisAdd BLAST21
Topological domaini142 – 159Cytoplasmic2 PublicationsAdd BLAST18
Transmembranei160 – 180HelicalSequence analysisAdd BLAST21
Topological domaini181 – 189Periplasmic1 Publication9
Transmembranei190 – 210HelicalSequence analysisAdd BLAST21
Topological domaini211 – 259Cytoplasmic1 PublicationAdd BLAST49
Transmembranei260 – 280HelicalSequence analysisAdd BLAST21
Topological domaini281 – 297Periplasmic1 PublicationAdd BLAST17
Transmembranei298 – 318HelicalSequence analysisAdd BLAST21
Topological domaini319 – 326Cytoplasmic1 Publication8
Transmembranei327 – 347HelicalSequence analysisAdd BLAST21
Topological domaini348 – 357Periplasmic1 Publication10
Transmembranei358 – 378HelicalSequence analysisAdd BLAST21
Topological domaini379 – 391Cytoplasmic1 PublicationAdd BLAST13
Transmembranei392 – 411HelicalSequence analysisAdd BLAST20
Topological domaini412 – 425Periplasmic1 PublicationAdd BLAST14
Transmembranei426 – 446HelicalSequence analysisAdd BLAST21
Topological domaini447 – 463Cytoplasmic1 Publication1 PublicationAdd BLAST17

GO - Cellular componenti

  • integral component of membrane Source: GO_Central
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi108C → S: No change in activity. 1 Publication1
Mutagenesisi143C → S: 30% of wild-type sugar phosphate transport activity. 1 Publication1
Mutagenesisi265C → S: No change in activity. 1 Publication1
Mutagenesisi331C → S: No change in activity. 1 Publication1
Mutagenesisi436C → S: No change in activity. 1 Publication1
Mutagenesisi438C → S: No change in activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001998831 – 463Hexose-6-phosphate:phosphate antiporterAdd BLAST463

Proteomic databases

PaxDbiP0AGC0.
PRIDEiP0AGC0.

Expressioni

Inductioni

Expression is induced by external glucose-6-phosphate through the UhpABC signaling cascade.4 Publications

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4262581. 5 interactors.
STRINGi511145.b3666.

Structurei

3D structure databases

ProteinModelPortaliP0AGC0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CXY. Bacteria.
COG2271. LUCA.
HOGENOMiHOG000274728.
InParanoidiP0AGC0.
KOiK07784.
OMAiWANIHAF.
PhylomeDBiP0AGC0.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR021159. Sugar-P_transporter_CS.
IPR000849. Sugar_P_transporter.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
PIRSFiPIRSF002808. Hexose_phosphate_transp. 1 hit.
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS00942. GLPT. 1 hit.
PS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AGC0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAFLNQVRK PTLDLPLEVR RKMWFKPFMQ SYLVVFIGYL TMYLIRKNFN
60 70 80 90 100
IAQNDMISTY GLSMTQLGMI GLGFSITYGV GKTLVSYYAD GKNTKQFLPF
110 120 130 140 150
MLILSAICML GFSASMGSGS VSLFLMIAFY ALSGFFQSTG GSCSYSTITK
160 170 180 190 200
WTPRRKRGTF LGFWNISHNL GGAGAAGVAL FGANYLFDGH VIGMFIFPSI
210 220 230 240 250
IALIVGFIGL RYGSDSPESY GLGKAEELFG EEISEEDKET ESTDMTKWQI
260 270 280 290 300
FVEYVLKNKV IWLLCFANIF LYVVRIGIDQ WSTVYAFQEL KLSKAVAIQG
310 320 330 340 350
FTLFEAGALV GTLLWGWLSD LANGRRGLVA CIALALIIAT LGVYQHASNE
360 370 380 390 400
YIYLASLFAL GFLVFGPQLL IGVAAVGFVP KKAIGAADGI KGTFAYLIGD
410 420 430 440 450
SFAKLGLGMI ADGTPVFGLT GWAGTFAALD IAAIGCICLM AIVAVMEERK
460
IRREKKIQQL TVA
Length:463
Mass (Da):50,607
Last modified:December 20, 2005 - v1
Checksum:i1BE23CEFF05311B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17102 Genomic DNA. Translation: AAA24723.1.
M89479 Genomic DNA. Translation: AAA24727.1.
L10328 Genomic DNA. Translation: AAA62018.1.
U00096 Genomic DNA. Translation: AAC76689.1.
AP009048 Genomic DNA. Translation: BAE77627.1.
PIRiA30395. MMECHP.
RefSeqiNP_418122.1. NC_000913.3.
WP_000879194.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76689; AAC76689; b3666.
BAE77627; BAE77627; BAE77627.
GeneIDi948201.
KEGGiecj:JW3641.
eco:b3666.
PATRICi32122825. VBIEscCol129921_3788.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17102 Genomic DNA. Translation: AAA24723.1.
M89479 Genomic DNA. Translation: AAA24727.1.
L10328 Genomic DNA. Translation: AAA62018.1.
U00096 Genomic DNA. Translation: AAC76689.1.
AP009048 Genomic DNA. Translation: BAE77627.1.
PIRiA30395. MMECHP.
RefSeqiNP_418122.1. NC_000913.3.
WP_000879194.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AGC0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262581. 5 interactors.
STRINGi511145.b3666.

Protein family/group databases

TCDBi2.A.1.4.1. the major facilitator superfamily (mfs).

Proteomic databases

PaxDbiP0AGC0.
PRIDEiP0AGC0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76689; AAC76689; b3666.
BAE77627; BAE77627; BAE77627.
GeneIDi948201.
KEGGiecj:JW3641.
eco:b3666.
PATRICi32122825. VBIEscCol129921_3788.

Organism-specific databases

EchoBASEiEB1047.
EcoGeneiEG11054. uhpT.

Phylogenomic databases

eggNOGiENOG4105CXY. Bacteria.
COG2271. LUCA.
HOGENOMiHOG000274728.
InParanoidiP0AGC0.
KOiK07784.
OMAiWANIHAF.
PhylomeDBiP0AGC0.

Enzyme and pathway databases

BioCyciEcoCyc:UHPT-MONOMER.
ECOL316407:JW3641-MONOMER.
MetaCyc:UHPT-MONOMER.

Miscellaneous databases

PROiP0AGC0.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR021159. Sugar-P_transporter_CS.
IPR000849. Sugar_P_transporter.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
PIRSFiPIRSF002808. Hexose_phosphate_transp. 1 hit.
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS00942. GLPT. 1 hit.
PS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUHPT_ECOLI
AccessioniPrimary (citable) accession number: P0AGC0
Secondary accession number(s): P13408, P76727, Q2M7X9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.