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Protein

RNA polymerase sigma factor RpoH

Gene

rpoH

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in regulation of expression of heat shock genes.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi253 – 27220H-T-H motifUniRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Sigma factor

Keywords - Biological processi

Stress response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciECOL386585:GJFA-4276-MONOMER.
ECOO157:RPOH-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase sigma factor RpoHUniRule annotation
Alternative name(s):
RNA polymerase sigma-32 factorUniRule annotation
Gene namesi
Name:rpoHUniRule annotation
Ordered Locus Names:Z4835, ECs4310
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 284284RNA polymerase sigma factor RpoHPRO_0000093958Add
BLAST

Interactioni

Subunit structurei

Interacts with the RNA polymerase core enzyme.UniRule annotation

Protein-protein interaction databases

MINTiMINT-1216175.
STRINGi155864.Z4835.

Structurei

3D structure databases

ProteinModelPortaliP0AGB5.
SMRiP0AGB5. Positions 35-279.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni53 – 12270Sigma-70 factor domain-2UniRule annotationAdd
BLAST
Regioni228 – 28053Sigma-70 factor domain-4UniRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi77 – 804Interaction with polymerase core subunit RpoC

Sequence similaritiesi

Belongs to the sigma-70 factor family. RpoH subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DEK. Bacteria.
COG0568. LUCA.
HOGENOMiHOG000270269.
KOiK03089.
OMAiLNNDEVH.
OrthoDBiEOG6D5G0W.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00961. Sigma70_RpoH.
InterProiIPR014284. RNA_pol_sigma-70_dom.
IPR000943. RNA_pol_sigma70.
IPR007627. RNA_pol_sigma70_r2.
IPR007630. RNA_pol_sigma70_r4.
IPR016263. RNA_pol_sigma_factor.
IPR013325. RNA_pol_sigma_r2.
IPR013324. RNA_pol_sigma_r3_r4.
IPR012759. RNA_pol_sigma_RpoH_proteobac.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF04542. Sigma70_r2. 1 hit.
PF04545. Sigma70_r4. 1 hit.
[Graphical view]
PIRSFiPIRSF000770. RNA_pol_sigma-SigE/K. 1 hit.
PRINTSiPR00046. SIGMA70FCT.
SUPFAMiSSF88659. SSF88659. 1 hit.
SSF88946. SSF88946. 1 hit.
TIGRFAMsiTIGR02392. rpoH_proteo. 1 hit.
TIGR02937. sigma70-ECF. 1 hit.
PROSITEiPS00715. SIGMA70_1. 1 hit.
PS00716. SIGMA70_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AGB5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDKMQSLAL APVGNLDSYI RAANAWPMLS ADEERALAEK LHYHGDLEAA
60 70 80 90 100
KTLILSHLRF VVHIARNYAG YGLPQADLIQ EGNIGLMKAV RRFNPEVGVR
110 120 130 140 150
LVSFAVHWIK AEIHEYVLRN WRIVKVATTK AQRKLFFNLR KTKQRLGWFN
160 170 180 190 200
QDEVEMVARE LGVTSKDVRE MESRMAAQDM TFDLSSDDDS DSQPMAPVLY
210 220 230 240 250
LQDKSSNFAD GIEDDNWEEQ AANRLTDAMQ GLDERSQDII RARWLDEDNK
260 270 280
STLQELADRY GVSAERVRQL EKNAMKKLRA AIEA
Length:284
Mass (Da):32,469
Last modified:December 20, 2005 - v1
Checksum:i628A17B4C835D00A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG58570.1.
BA000007 Genomic DNA. Translation: BAB37733.1.
PIRiF86013.
F91167.
RefSeqiNP_312337.1. NC_002695.1.
WP_000130217.1. NZ_LPWC01000207.1.

Genome annotation databases

EnsemblBacteriaiAAG58570; AAG58570; Z4835.
BAB37733; BAB37733; BAB37733.
GeneIDi915834.
KEGGiece:Z4835.
ecs:ECs4310.
PATRICi18358189. VBIEscCol44059_4261.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG58570.1.
BA000007 Genomic DNA. Translation: BAB37733.1.
PIRiF86013.
F91167.
RefSeqiNP_312337.1. NC_002695.1.
WP_000130217.1. NZ_LPWC01000207.1.

3D structure databases

ProteinModelPortaliP0AGB5.
SMRiP0AGB5. Positions 35-279.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1216175.
STRINGi155864.Z4835.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG58570; AAG58570; Z4835.
BAB37733; BAB37733; BAB37733.
GeneIDi915834.
KEGGiece:Z4835.
ecs:ECs4310.
PATRICi18358189. VBIEscCol44059_4261.

Phylogenomic databases

eggNOGiENOG4105DEK. Bacteria.
COG0568. LUCA.
HOGENOMiHOG000270269.
KOiK03089.
OMAiLNNDEVH.
OrthoDBiEOG6D5G0W.

Enzyme and pathway databases

BioCyciECOL386585:GJFA-4276-MONOMER.
ECOO157:RPOH-MONOMER.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00961. Sigma70_RpoH.
InterProiIPR014284. RNA_pol_sigma-70_dom.
IPR000943. RNA_pol_sigma70.
IPR007627. RNA_pol_sigma70_r2.
IPR007630. RNA_pol_sigma70_r4.
IPR016263. RNA_pol_sigma_factor.
IPR013325. RNA_pol_sigma_r2.
IPR013324. RNA_pol_sigma_r3_r4.
IPR012759. RNA_pol_sigma_RpoH_proteobac.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF04542. Sigma70_r2. 1 hit.
PF04545. Sigma70_r4. 1 hit.
[Graphical view]
PIRSFiPIRSF000770. RNA_pol_sigma-SigE/K. 1 hit.
PRINTSiPR00046. SIGMA70FCT.
SUPFAMiSSF88659. SSF88659. 1 hit.
SSF88946. SSF88946. 1 hit.
TIGRFAMsiTIGR02392. rpoH_proteo. 1 hit.
TIGR02937. sigma70-ECF. 1 hit.
PROSITEiPS00715. SIGMA70_1. 1 hit.
PS00716. SIGMA70_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiRPOH_ECO57
AccessioniPrimary (citable) accession number: P0AGB5
Secondary accession number(s): P00580
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: December 20, 2005
Last modified: May 11, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.