P0AGB0 (SERB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoserine phosphatase
Short name=PSP
Short name=PSPase
EC=3.1.3.3

Alternative name(s):
O-phosphoserine phosphohydrolase

Gene names

Name:	serB
Ordered Locus Names:	b4388, JW4351

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	O-phospho-L(or D)-serine + H2O = L(or D)-serine + phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.
Sequence similarities	Belongs to the serB family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Serine biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from direct assay. Source: EcoliWiki
Cellular component	cytoplasm Inferred from direct assay. Source: EcoliWiki
Molecular function	magnesium ion binding Inferred from direct assay. Source: EcoliWiki phosphoserine phosphatase activity Inferred from direct assay. Source: EcoCyc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
nadE	P18843	1	EBI-1126007,EBI-548960

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	Phosphoserine phosphatase		PRO_0000156886
Regions
	Region	204 – 205	2	Substrate binding By similarity
Sites
	Active site	116	1	Nucleophile By similarity
	Active site	118	1	Proton donor By similarity
	Metal binding	116	1	Magnesium By similarity
	Metal binding	118	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	272	1	Magnesium By similarity
	Binding site	125	1	Substrate By similarity
	Binding site	161	1	Substrate By similarity
	Binding site	249	1	Substrate By similarity
	Binding site	275	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0AGB0 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: A09A43BD846B7DE6	FASTA	322	35,043
	10 20 30 40 50 60 MPNITWCDLP EDVSLWPGLP LSLSGDEVMP LDYHAGRSGW LLYGRGLDKQ RLTQYQSKLG 70 80 90 100 110 120 AAMVIVAAWC VEDYQVIRLA GSLTARATRL AHEAQLDVAP LGKIPHLRTP GLLVMDMDST 130 140 150 160 170 180 AIQIECIDEI AKLAGTGEMV AEVTERAMRG ELDFTASLRS RVATLKGADA NILQQVRENL 190 200 210 220 230 240 PLMPGLTQLV LKLETLGWKV AIASGGFTFF AEYLRDKLRL TAVVANELEI MDGKFTGNVI 250 260 270 280 290 300 GDIVDAQYKA KTLTRLAQEY EIPLAQTVAI GDGANDLPMI KAAGLGIAYH AKPKVNEKAE 310 320 VTIRHADLMG VFCILSGSLN QK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence and characterization of the Escherichia coli serB gene." Neuwald A.F., Stauffer G.V. Nucleic Acids Res. 13:7025-7039(1985) [PubMed: 2997734] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03046 Genomic DNA. Translation: CAA26852.1. U14003 Genomic DNA. Translation: AAA97284.1. U00096 Genomic DNA. Translation: AAC77341.1. AP009048 Genomic DNA. Translation: BAE78377.1.
PIR	PAECS. A24271.
RefSeq	NP_418805.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0AGB0.
SMR	P0AGB0. Positions 11-322.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48100N.
IntAct	P0AGB0. 3 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000005062; EBESCP00000005062; EBESCG00000004128. EBESCT00000015561; EBESCP00000014852; EBESCG00000014621.
GeneID	948913.
GenomeReviews	Gene locus JW4351 in contig AP009048_GR. Gene locus b4388 in contig U00096_GR.
KEGG	ecj:JW4351. eco:b4388.
PATRIC	32124392. VBIEscCol129921_4536.
Organism-specific databases
EchoBASE	EB0938.
EcoGene	EG10945. serB.
Phylogenomic databases
eggNOG	COG0560.
GeneTree	EBGT00050000011712.
HOGENOM	HBG645339.
OMA	DYQVVRL.
PhylomeDB	P0AGB0.
ProtClustDB	PRK11133.
Enzyme and pathway databases
BioCyc	EcoCyc:PSERPHOSPHA-MONOMER. MetaCyc:PSERPHOSPHA-MONOMER.
Gene expression databases
Genevestigator	P0AGB0.
Family and domain databases
InterPro	IPR005834. Dehalogen-like_hydro. IPR023214. HAD-like_dom. IPR006383. HAD-SF_hydro_IB_PSP-like. IPR023190. Pser_Pase_dom_2. IPR004469. SerB. [Graphical view]
Gene3D	G3DSA:3.40.50.1000. HAD-like_dom. 2 hits. G3DSA:1.10.150.210. Pser_Pase_dom_2. 1 hit.
KO	K01079.
Pfam	PF00702. Hydrolase. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01488. HAD-SF-IB. 1 hit. TIGR00338. SerB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SERB_ECOLI

Accession

Primary (citable) accession number: P0AGB0
Secondary accession number(s): P06862, Q2M5S9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program