Reviewed, UniProtKB/Swiss-Prot P0AGB0 (SERB_ECOLI)

Names and origin

Protein names

Recommended name:
    Phosphoserine phosphatase
      Short name=PSPase
      Short name=PSP
    EC=3.1.3.3
Alternative name(s):
    O-phosphoserine phosphohydrolase

Gene names

Name:	serB
Ordered Locus Names:	b4388, JW4351

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	O-phospho-L(or D)-serine + H(2)O = L(or D)-serine + phosphate.
Sequence similarities	Belongs to the serB family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Serine biosynthesis
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism Inferred from electronic annotation. Source: InterPro phosphoserine phosphatase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
nadE	P18843	1	EBI-1126007,EBI-548960

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	Phosphoserine phosphatase		PRO_0000156886

Sequences

	Sequence		Length	Mass (Da)
	P0AGB0-1 [UniParc]. Last modified January 1, 1988. Version 1. Checksum: A09A43BD846B7DE6	FASTA	322	35,043
	10 20 30 40 50 60 MPNITWCDLP EDVSLWPGLP LSLSGDEVMP LDYHAGRSGW LLYGRGLDKQ RLTQYQSKLG 70 80 90 100 110 120 AAMVIVAAWC VEDYQVIRLA GSLTARATRL AHEAQLDVAP LGKIPHLRTP GLLVMDMDST 130 140 150 160 170 180 AIQIECIDEI AKLAGTGEMV AEVTERAMRG ELDFTASLRS RVATLKGADA NILQQVRENL 190 200 210 220 230 240 PLMPGLTQLV LKLETLGWKV AIASGGFTFF AEYLRDKLRL TAVVANELEI MDGKFTGNVI 250 260 270 280 290 300 GDIVDAQYKA KTLTRLAQEY EIPLAQTVAI GDGANDLPMI KAAGLGIAYH AKPKVNEKAE 310 320 VTIRHADLMG VFCILSGSLN QK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence and characterization of the Escherichia coli serB gene." Neuwald A.F., Stauffer G.V. Nucleic Acids Res. 13:7025-7039(1985) [PubMed: 2997734] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X03046 Genomic DNA. Translation: CAA26852.1. U14003 Genomic DNA. Translation: AAA97284.1. U00096 Genomic DNA. Translation: AAC77341.1. AP009048 Genomic DNA. Translation: BAE78377.1.
PIR	PAECS. A24271.
RefSeq	AP_004876.1. NP_418805.1.
3D structure databases
HSSP	HSSP built from PDB template 1J97 based on UniProtKB Q58989.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0AGB0.
Genome annotation databases
GeneID	948913.
GenomeReviews	Gene locus b4388 in contig U00096_GR. Gene locus JW4351 in contig AP009048_GR.
KEGG	ecj:JW4351. eco:b4388.
Organism-specific databases
EchoBASE	EB0938.
EcoGene	EG10945. serB.
CMR	Search...
Phylogenomic databases
HOGENOM	P0AGB0.
Enzyme and pathway databases
BioCyc	EcoCyc:PSERPHOSPHA-MON.
Family and domain databases
InterPro	IPR001757. ATPase_P. IPR005834. Dehalogen-like_hydro. IPR006383. HAD-SF_hydro_IB_PSP-like. IPR004469. SerB. [Graphical view]
Pfam	PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE.
TIGRFAMs	TIGR01488. HAD-SF-IB. 1 hit. TIGR00338. serB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SERB_ECOLI

Accession

Primary (citable) accession number: P0AGB0
Secondary accession number(s): P06862, Q2M5S9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	January 1, 1988
Last modified:	November 25, 2008
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)