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Protein

Transcriptional regulatory protein UhpA

Gene

uhpA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the UhpABC signaling cascade that controls the expression of the hexose phosphate transporter UhpT. Activates the transcription of the uhpT gene. Acts by binding specifically to the uhpT promoter region.2 Publications

Enzyme regulationi

Phosphorylation by UhpB enhances DNA binding activity.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi155 – 17420H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:UHPA-MONOMER.
ECOL316407:JW3644-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein UhpACurated
Gene namesi
Name:uhpA
Ordered Locus Names:b3669, JW3644
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11051. uhpA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi54 – 541D → N: Lack of phosphorylation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 196196Transcriptional regulatory protein UhpAPRO_0000081257Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei54 – 5414-aspartylphosphatePROSITE-ProRule annotation1 Publication

Post-translational modificationi

Phosphorylated and dephosphorylated by UhpB.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0AGA6.

Interactioni

Protein-protein interaction databases

BioGridi4262584. 22 interactions.
DIPiDIP-11082N.
IntActiP0AGA6. 4 interactions.
STRINGi511145.b3669.

Structurei

3D structure databases

ProteinModelPortaliP0AGA6.
SMRiP0AGA6. Positions 1-189.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 116114Response regulatoryPROSITE-ProRule annotationAdd
BLAST
Domaini131 – 19666HTH luxR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH luxR-type DNA-binding domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108KX4. Bacteria.
COG2197. LUCA.
HOGENOMiHOG000034813.
InParanoidiP0AGA6.
KOiK07686.
OMAiCIMLSVH.
OrthoDBiEOG69GZGV.
PhylomeDBiP0AGA6.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00196. GerE. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AGA6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITVALIDDH LIVRSGFAQL LGLEPDLQVV AEFGSGREAL AGLPGRGVQV
60 70 80 90 100
CICDISMPDI SGLELLSQLP KGMATIMLSV HDSPALVEQA LNAGARGFLS
110 120 130 140 150
KRCSPDELIA AVHTVATGGC YLTPDIAIKL ASGRQDPLTK RERQVAEKLA
160 170 180 190
QGMAVKEIAA ELGLSPKTVH VHRANLMEKL GVSNDVELAR RMFDGW
Length:196
Mass (Da):20,889
Last modified:December 20, 2005 - v1
Checksum:i8F409F8AADE08276
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17102 Genomic DNA. Translation: AAA24720.1.
M89479 Genomic DNA. Translation: AAA24724.1.
L10328 Genomic DNA. Translation: AAA62021.1.
U00096 Genomic DNA. Translation: AAC76692.1.
AP009048 Genomic DNA. Translation: BAE77624.1.
PIRiA26925. BVECAU.
RefSeqiNP_418125.1. NC_000913.3.
WP_000633668.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76692; AAC76692; b3669.
BAE77624; BAE77624; BAE77624.
GeneIDi948178.
KEGGiecj:JW3644.
eco:b3669.
PATRICi32122831. VBIEscCol129921_3791.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17102 Genomic DNA. Translation: AAA24720.1.
M89479 Genomic DNA. Translation: AAA24724.1.
L10328 Genomic DNA. Translation: AAA62021.1.
U00096 Genomic DNA. Translation: AAC76692.1.
AP009048 Genomic DNA. Translation: BAE77624.1.
PIRiA26925. BVECAU.
RefSeqiNP_418125.1. NC_000913.3.
WP_000633668.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AGA6.
SMRiP0AGA6. Positions 1-189.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262584. 22 interactions.
DIPiDIP-11082N.
IntActiP0AGA6. 4 interactions.
STRINGi511145.b3669.

Proteomic databases

PaxDbiP0AGA6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76692; AAC76692; b3669.
BAE77624; BAE77624; BAE77624.
GeneIDi948178.
KEGGiecj:JW3644.
eco:b3669.
PATRICi32122831. VBIEscCol129921_3791.

Organism-specific databases

EchoBASEiEB1044.
EcoGeneiEG11051. uhpA.

Phylogenomic databases

eggNOGiENOG4108KX4. Bacteria.
COG2197. LUCA.
HOGENOMiHOG000034813.
InParanoidiP0AGA6.
KOiK07686.
OMAiCIMLSVH.
OrthoDBiEOG69GZGV.
PhylomeDBiP0AGA6.

Enzyme and pathway databases

BioCyciEcoCyc:UHPA-MONOMER.
ECOL316407:JW3644-MONOMER.

Miscellaneous databases

PROiP0AGA6.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00196. GerE. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the uhp region of Escherichia coli."
    Friedrich M.J., Kadner R.J.
    J. Bacteriol. 169:3556-3563(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Structure and function of the uhp genes for the sugar phosphate transport system in Escherichia coli and Salmonella typhimurium."
    Island M.D., Wei B.-Y., Kadner R.J.
    J. Bacteriol. 174:2754-2762(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
    Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
    Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Protein phosphorylation affects binding of the Escherichia coli transcription activator UhpA to the uhpT promoter."
    Dahl J.L., Wei B.Y., Kadner R.J.
    J. Biol. Chem. 272:1910-1919(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-20, FUNCTION, ENZYME REGULATION, PHOSPHORYLATION AT ASP-54, MUTAGENESIS OF ASP-54.
  7. "Identification of uhp polypeptides and evidence for their role in exogenous induction of the sugar phosphate transport system of Escherichia coli K-12."
    Weston L.A., Kadner R.J.
    J. Bacteriol. 169:3546-3555(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.
  9. "The histidine kinase domain of UhpB inhibits UhpA action at the Escherichia coli uhpT promoter."
    Wright J.S., Olekhnovich I.N., Touchie G., Kadner R.J.
    J. Bacteriol. 182:6279-6286(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION BY UHPB.
  10. "Glucose-6-phosphate-dependent phosphoryl flow through the Uhp two-component regulatory system."
    Verhamme D.T., Arents J.C., Postma P.W., Crielaard W., Hellingwerf K.J.
    Microbiology 147:3345-3352(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AND DEPHOSPHORYLATION BY UHPB.

Entry informationi

Entry nameiUHPA_ECOLI
AccessioniPrimary (citable) accession number: P0AGA6
Secondary accession number(s): P10940, Q2M7Y2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: May 11, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.