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Protein

Transcriptional regulatory protein UhpA

Gene

uhpA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the UhpABC signaling cascade that controls the expression of the hexose phosphate transporter UhpT. Activates the transcription of the uhpT gene. Acts by binding specifically to the uhpT promoter region.2 Publications

Enzyme regulationi

Phosphorylation by UhpB enhances DNA binding activity.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi155 – 174H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

  • DNA binding Source: EcoCyc

GO - Biological processi

  • phosphorelay signal transduction system Source: UniProtKB-KW
  • regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:UHPA-MONOMER.
ECOL316407:JW3644-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein UhpACurated
Gene namesi
Name:uhpA
Ordered Locus Names:b3669, JW3644
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11051. uhpA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi54D → N: Lack of phosphorylation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000812571 – 196Transcriptional regulatory protein UhpAAdd BLAST196

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei544-aspartylphosphatePROSITE-ProRule annotation1 Publication1

Post-translational modificationi

Phosphorylated and dephosphorylated by UhpB.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0AGA6.
PRIDEiP0AGA6.

Interactioni

Protein-protein interaction databases

BioGridi4262584. 22 interactors.
DIPiDIP-11082N.
IntActiP0AGA6. 4 interactors.
STRINGi511145.b3669.

Structurei

3D structure databases

ProteinModelPortaliP0AGA6.
SMRiP0AGA6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 116Response regulatoryPROSITE-ProRule annotationAdd BLAST114
Domaini131 – 196HTH luxR-typePROSITE-ProRule annotationAdd BLAST66

Sequence similaritiesi

Contains 1 HTH luxR-type DNA-binding domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108KX4. Bacteria.
COG2197. LUCA.
HOGENOMiHOG000034813.
InParanoidiP0AGA6.
KOiK07686.
OMAiCIMLSVH.
PhylomeDBiP0AGA6.

Family and domain databases

CDDicd06170. LuxR_C_like. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00196. GerE. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0AGA6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITVALIDDH LIVRSGFAQL LGLEPDLQVV AEFGSGREAL AGLPGRGVQV
60 70 80 90 100
CICDISMPDI SGLELLSQLP KGMATIMLSV HDSPALVEQA LNAGARGFLS
110 120 130 140 150
KRCSPDELIA AVHTVATGGC YLTPDIAIKL ASGRQDPLTK RERQVAEKLA
160 170 180 190
QGMAVKEIAA ELGLSPKTVH VHRANLMEKL GVSNDVELAR RMFDGW
Length:196
Mass (Da):20,889
Last modified:December 20, 2005 - v1
Checksum:i8F409F8AADE08276
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17102 Genomic DNA. Translation: AAA24720.1.
M89479 Genomic DNA. Translation: AAA24724.1.
L10328 Genomic DNA. Translation: AAA62021.1.
U00096 Genomic DNA. Translation: AAC76692.1.
AP009048 Genomic DNA. Translation: BAE77624.1.
PIRiA26925. BVECAU.
RefSeqiNP_418125.1. NC_000913.3.
WP_000633668.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76692; AAC76692; b3669.
BAE77624; BAE77624; BAE77624.
GeneIDi948178.
KEGGiecj:JW3644.
eco:b3669.
PATRICi32122831. VBIEscCol129921_3791.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17102 Genomic DNA. Translation: AAA24720.1.
M89479 Genomic DNA. Translation: AAA24724.1.
L10328 Genomic DNA. Translation: AAA62021.1.
U00096 Genomic DNA. Translation: AAC76692.1.
AP009048 Genomic DNA. Translation: BAE77624.1.
PIRiA26925. BVECAU.
RefSeqiNP_418125.1. NC_000913.3.
WP_000633668.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AGA6.
SMRiP0AGA6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262584. 22 interactors.
DIPiDIP-11082N.
IntActiP0AGA6. 4 interactors.
STRINGi511145.b3669.

Proteomic databases

PaxDbiP0AGA6.
PRIDEiP0AGA6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76692; AAC76692; b3669.
BAE77624; BAE77624; BAE77624.
GeneIDi948178.
KEGGiecj:JW3644.
eco:b3669.
PATRICi32122831. VBIEscCol129921_3791.

Organism-specific databases

EchoBASEiEB1044.
EcoGeneiEG11051. uhpA.

Phylogenomic databases

eggNOGiENOG4108KX4. Bacteria.
COG2197. LUCA.
HOGENOMiHOG000034813.
InParanoidiP0AGA6.
KOiK07686.
OMAiCIMLSVH.
PhylomeDBiP0AGA6.

Enzyme and pathway databases

BioCyciEcoCyc:UHPA-MONOMER.
ECOL316407:JW3644-MONOMER.

Miscellaneous databases

PROiP0AGA6.

Family and domain databases

CDDicd06170. LuxR_C_like. 1 hit.
Gene3Di1.10.10.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR016032. Sig_transdc_resp-reg_C-effctor.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00196. GerE. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00038. HTHLUXR.
SMARTiSM00421. HTH_LUXR. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46894. SSF46894. 1 hit.
SSF52172. SSF52172. 1 hit.
PROSITEiPS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUHPA_ECOLI
AccessioniPrimary (citable) accession number: P0AGA6
Secondary accession number(s): P10940, Q2M7Y2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.