Reviewed,
UniProtKB/Swiss-Prot P0AGA2 (SECY_ECOLI)
Last modified
December 15, 2009.
Version 39.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Preprotein translocase subunit secY | ||||||
| Gene names |
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| Organism | Escherichia coli (strain K12) [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 83333 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 443 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in protein export. Interacts with secA and secE to allow the translocation of proteins across the plasma membrane, by forming part of a channel. |
| Subunit structure | One of seven secretory proteins (secA-F and secY) that comprise the prokaryotic protein translocation apparatus. |
| Subcellular location | |
| Sequence similarities | Belongs to the secY/SEC61-alpha family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Protein transport Translocation Transport |
| Cellular component | Cell inner membrane Cell membrane Membrane |
| Domain | Transmembrane |
| Technical term | 3D-structure Complete proteome |
| Gene Ontology (GO) | |
| Biological process | protein transport Inferred from electronic annotation. Source: UniProtKB-KW transmembrane transportInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | P-P-bond-hydrolysis-driven protein transmembrane transporter activity Inferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 443 | 443 | Preprotein translocase subunit secY | PRO_0000131721 | |||||
Regions | |||||||||
| Topological domain | 1 – 21 | 21 | Cytoplasmic Probable | ||||||
| Transmembrane | 22 – 43 | 22 | Probable | ||||||
| Topological domain | 44 – 77 | 34 | Periplasmic Probable | ||||||
| Transmembrane | 78 – 98 | 21 | Probable | ||||||
| Topological domain | 99 – 121 | 23 | Cytoplasmic Probable | ||||||
| Transmembrane | 122 – 138 | 17 | Probable | ||||||
| Topological domain | 139 – 157 | 19 | Periplasmic Probable | ||||||
| Transmembrane | 158 – 175 | 18 | Probable | ||||||
| Topological domain | 176 – 183 | 8 | Cytoplasmic Probable | ||||||
| Transmembrane | 184 – 205 | 22 | Probable | ||||||
| Topological domain | 206 – 214 | 9 | Periplasmic Probable | ||||||
| Transmembrane | 215 – 238 | 24 | Probable | ||||||
| Topological domain | 239 – 270 | 32 | Cytoplasmic Probable | ||||||
| Transmembrane | 271 – 292 | 22 | Probable | ||||||
| Topological domain | 293 – 313 | 21 | Periplasmic Probable | ||||||
| Transmembrane | 314 – 336 | 23 | Probable | ||||||
| Topological domain | 337 – 371 | 35 | Cytoplasmic Probable | ||||||
| Transmembrane | 372 – 391 | 20 | Probable | ||||||
| Topological domain | 392 – 395 | 4 | Periplasmic Probable | ||||||
| Transmembrane | 396 – 419 | 24 | Probable | ||||||
| Topological domain | 420 – 443 | 24 | Cytoplasmic Probable | ||||||
Experimental info | |||||||||
| Mutagenesis | 40 | 1 | P → S in secY100; temperature-sensitive. | ||||||
| Mutagenesis | 67 | 1 | F → C in prlA3; alterated signal sequence interaction. | ||||||
| Mutagenesis | 167 | 1 | G → E in secY100; temperature-sensitive. | ||||||
| Mutagenesis | 240 | 1 | G → D in secY24; temperature-sensitive. | ||||||
| Mutagenesis | 282 | 1 | S → R in prlA401; alterated signal sequence interaction. | ||||||
| Mutagenesis | 286 | 1 | F → Y in prlA4-1; alterated signal sequence interaction. | ||||||
| Mutagenesis | 287 | 1 | P → L in secY161; alterated signal sequence interaction. | ||||||
| Mutagenesis | 290 | 1 | I → T in secY121; alterated signal sequence interaction. | ||||||
| Mutagenesis | 357 | 1 | R → H in secY39; cold-sensitive. | ||||||
| Mutagenesis | 363 | 1 | A → S in secY40; cold-sensitive. | ||||||
| Mutagenesis | 408 | 1 | I → N in prlA4-2; alterated signal sequence interaction. | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene." Cerretti D.P., Dean D., Davis G.R., Bedwell D.M., Nomura M. Nucleic Acids Res. 11:2599-2616(1983) [PubMed: 6222285] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12. |
| [2] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [3] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [4] | "Topology analysis of the SecY protein, an integral membrane protein involved in protein export in Escherichia coli." Akiyama Y., Ito K. EMBO J. 6:3465-3470(1987) [PubMed: 2828030] [Abstract] Cited for: TOPOLOGY. |
| [5] | "Biochemical analysis of the biogenesis and function of the Escherichia coli export factor SecY." Swidersky U.E., Rienhoefer-Schweer A., Werner P.K., Ernst F., Benson S.A., Hoffschulte H.K., Mueller M. Eur. J. Biochem. 207:803-811(1992) [PubMed: 1633829] [Abstract] Cited for: CHARACTERIZATION. |
| [6] | "Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076. |
| [7] | "Structure, function, and biogenesis of SecY, an integral membrane protein involved in protein export." Ito K. J. Bioenerg. Biomembr. 22:353-367(1990) [PubMed: 2202723] [Abstract] Cited for: REVIEW. |
| [8] | "Temperature-sensitive sec mutants of Escherichia coli: inhibition of protein export at the permissive temperature." Ito K., Hirota Y., Akiyama Y. J. Bacteriol. 171:1742-1743(1989) [PubMed: 2646297] [Abstract] Cited for: MUTANT SECY100. |
| [9] | "Characterization of cold-sensitive secY mutants of Escherichia coli." Baba T., Jacq A., Brickman E., Beckwith J., Taura T., Ueguchi C., Akiyama Y., Ito K. J. Bacteriol. 172:7005-7010(1990) [PubMed: 2254269] [Abstract] Cited for: MUTANTS SECY39 AND SECY40. |
| [10] | "Novel prlA alleles defective in supporting staphylokinase processing in Escherichia coli." Sako T. J. Bacteriol. 173:2289-2296(1991) [PubMed: 2007553] [Abstract] Cited for: MUTANTS SECY121 AND SECY161. |
| [11] | "PrlA suppressor mutations cluster in regions corresponding to three distinct topological domains." Osborne R.S., Silhavy T.J. EMBO J. 12:3391-3398(1993) [PubMed: 8253067] [Abstract] Cited for: MUTANTS. |
| [12] | "The allele-specific synthetic lethality of prlA-prlG double mutants predicts interactive domains of SecY and SecE." Flower A.M., Osborne R.S., Silhavy T.J. EMBO J. 14:884-893(1995) [PubMed: 7889938] [Abstract] Cited for: MUTANTS. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| X01563 Genomic DNA. Translation: CAA25725.1. U18997 Genomic DNA. Translation: AAA58097.1. U00096 Genomic DNA. Translation: AAC76325.1. AP009048 Genomic DNA. Translation: BAE77991.1. | |||||||||||||||||||
| PIR | QQECSY. A04473. | ||||||||||||||||||
| RefSeq | AP_004490.1. NP_417759.1. | ||||||||||||||||||
3D structure databases | |||||||||||||||||||
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| ModBase | Search... | ||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||
| IntAct | P0AGA2. 1 interaction. | ||||||||||||||||||
| STRING | P0AGA2. | ||||||||||||||||||
Protein family/group databases | |||||||||||||||||||
| TCDB | 3.A.5.1.1. general secretory pathway (Sec) family. | ||||||||||||||||||
Genome annotation databases | |||||||||||||||||||
| GeneID | 947799. | ||||||||||||||||||
| GenomeReviews | Gene locus JW3262 in contig AP009048_GR. Gene locus b3300 in contig U00096_GR. | ||||||||||||||||||
| KEGG | ecj:JW3262. eco:b3300. | ||||||||||||||||||
Organism-specific databases | |||||||||||||||||||
| EchoBASE | EB0759. | ||||||||||||||||||
| EcoGene | EG10766. secY. | ||||||||||||||||||
| CMR | Search... | ||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||
| HOGENOM | HBG565931. | ||||||||||||||||||
| OMA | IQTYVIS. | ||||||||||||||||||
Enzyme and pathway databases | |||||||||||||||||||
| BioCyc | EcoCyc:SECY. ECOL168927:B3300-MON. | ||||||||||||||||||
Gene expression databases | |||||||||||||||||||
| Genevestigator | P0AGA2. | ||||||||||||||||||
Family and domain databases | |||||||||||||||||||
| InterPro | IPR002208. SecY. [Graphical view] | ||||||||||||||||||
| PANTHER | PTHR10906. SecY. 1 hit. | ||||||||||||||||||
| Pfam | PF00344. SecY. 1 hit. [Graphical view] | ||||||||||||||||||
| PIRSF | PIRSF004557. SecY. 1 hit. | ||||||||||||||||||
| PRINTS | PR00303. SECYTRNLCASE. | ||||||||||||||||||
| PROSITE | PS00755. SECY_1. 1 hit. PS00756. SECY_2. 1 hit. [Graphical view] | ||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||
Entry information
| Entry name | SECY_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P0AGA2 Secondary accession number(s): P03844, Q2M6W5 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

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