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Protein

Protein-export membrane protein SecG

Gene

secG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of the protein translocation channel SecYEG. Overexpression of some hybrid proteins has been thought to jam the protein secretion apparatus resulting in cell death; while this may be true it also results in FtsH-mediated degradation of SecY. Treatment with antibiotics that block translation elongation such as chloramphenicol also leads to degradation of SecY and SecE but not SecG.

GO - Molecular functioni

GO - Biological processi

  • intracellular protein transmembrane transport Source: EcoliWiki
  • intracellular protein transport Source: EcoliWiki
  • protein secretion Source: InterPro
  • protein transport by the Sec complex Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciEcoCyc:SECG.
ECOL316407:JW3142-MONOMER.

Protein family/group databases

TCDBi3.A.5.1.1. the general secretory pathway (sec) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-export membrane protein SecG
Alternative name(s):
P12
Preprotein translocase band 1 subunit
Gene namesi
Name:secG
Ordered Locus Names:b3175, JW3142
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12095. secG.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1313Periplasmic1 PublicationAdd
BLAST
Transmembranei14 – 2512HelicalCuratedAdd
BLAST
Topological domaini26 – 5025Cytoplasmic1 PublicationAdd
BLAST
Transmembranei51 – 6616HelicalCuratedAdd
BLAST
Topological domaini67 – 11044Periplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi41 – 411T → P: Affects activity.
Mutagenesisi42 – 421L → P: Affects activity.
Mutagenesisi43 – 431F → S or Y: Affects activity.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 110110Protein-export membrane protein SecGPRO_0000157225Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PaxDbiP0AG99.
PRIDEiP0AG99.

Interactioni

Subunit structurei

Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. SecG probably contacts ribosomal protein L23 and/or L29 when the translocation complex is docked with the ribosome.

Binary interactionsi

WithEntry#Exp.IntActNotes
secAP104082EBI-6404248,EBI-543213

Protein-protein interaction databases

BioGridi4259584. 309 interactions.
DIPiDIP-47480N.
IntActiP0AG99. 2 interactions.
MINTiMINT-6477974.
STRINGi511145.b3175.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AKHelectron microscopy14.90A/X1-77[»]
2AKIelectron microscopy14.90A/X1-77[»]
3J45electron microscopy9.50G9-73[»]
3J46electron microscopy10.10G9-73[»]
ProteinModelPortaliP0AG99.
SMRiP0AG99. Positions 9-73.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AG99.

Family & Domainsi

Sequence similaritiesi

Belongs to the SecG family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105NTZ. Bacteria.
COG1314. LUCA.
HOGENOMiHOG000071344.
InParanoidiP0AG99.
KOiK03075.
OMAiWENLQQT.
PhylomeDBiP0AG99.

Family and domain databases

InterProiIPR004692. SecG.
[Graphical view]
PfamiPF03840. SecG. 1 hit.
[Graphical view]
PRINTSiPR01651. SECGEXPORT.
TIGRFAMsiTIGR00810. secG. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AG99-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYEALLVVFL IVAIGLVGLI MLQQGKGADM GASFGAGASA TLFGSSGSGN
60 70 80 90 100
FMTRMTALLA TLFFIISLVL GNINSNKTNK GSEWENLSAP AKTEQTQPAA
110
PAKPTSDIPN
Length:110
Mass (Da):11,365
Last modified:December 20, 2005 - v1
Checksum:i8DC7663F94D32F2F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16463 Genomic DNA. Translation: BAA03930.1.
L12968 Unassigned DNA. No translation available.
U01376 Genomic DNA. Translation: AAA97511.1.
U18997 Genomic DNA. Translation: AAA57976.1.
U00096 Genomic DNA. Translation: AAC76207.1.
AP009048 Genomic DNA. Translation: BAE77219.1.
PIRiS40402.
RefSeqiNP_417642.1. NC_000913.3.
WP_001295556.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76207; AAC76207; b3175.
BAE77219; BAE77219; BAE77219.
GeneIDi947720.
KEGGiecj:JW3142.
eco:b3175.
PATRICi32121768. VBIEscCol129921_3268.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16463 Genomic DNA. Translation: BAA03930.1.
L12968 Unassigned DNA. No translation available.
U01376 Genomic DNA. Translation: AAA97511.1.
U18997 Genomic DNA. Translation: AAA57976.1.
U00096 Genomic DNA. Translation: AAC76207.1.
AP009048 Genomic DNA. Translation: BAE77219.1.
PIRiS40402.
RefSeqiNP_417642.1. NC_000913.3.
WP_001295556.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AKHelectron microscopy14.90A/X1-77[»]
2AKIelectron microscopy14.90A/X1-77[»]
3J45electron microscopy9.50G9-73[»]
3J46electron microscopy10.10G9-73[»]
ProteinModelPortaliP0AG99.
SMRiP0AG99. Positions 9-73.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259584. 309 interactions.
DIPiDIP-47480N.
IntActiP0AG99. 2 interactions.
MINTiMINT-6477974.
STRINGi511145.b3175.

Protein family/group databases

TCDBi3.A.5.1.1. the general secretory pathway (sec) family.

Proteomic databases

PaxDbiP0AG99.
PRIDEiP0AG99.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76207; AAC76207; b3175.
BAE77219; BAE77219; BAE77219.
GeneIDi947720.
KEGGiecj:JW3142.
eco:b3175.
PATRICi32121768. VBIEscCol129921_3268.

Organism-specific databases

EchoBASEiEB2019.
EcoGeneiEG12095. secG.

Phylogenomic databases

eggNOGiENOG4105NTZ. Bacteria.
COG1314. LUCA.
HOGENOMiHOG000071344.
InParanoidiP0AG99.
KOiK03075.
OMAiWENLQQT.
PhylomeDBiP0AG99.

Enzyme and pathway databases

BioCyciEcoCyc:SECG.
ECOL316407:JW3142-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AG99.
PROiP0AG99.

Family and domain databases

InterProiIPR004692. SecG.
[Graphical view]
PfamiPF03840. SecG. 1 hit.
[Graphical view]
PRINTSiPR01651. SECGEXPORT.
TIGRFAMsiTIGR00810. secG. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSECG_ECOLI
AccessioniPrimary (citable) accession number: P0AG99
Secondary accession number(s): P33582, Q2M937
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: September 7, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.