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Protein

Protein translocase subunit SecF

Gene

secF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA. The large periplasmic domain is thought to have a base and head domain joined by a hinge; movement of the hinge may be coupled to both proton transport and protein export, with the head domain capturing substrate, and a conformational change preventing backward movement and driving forward movement. Expression of V.alginolyticus SecD and SecF in E.coli confers Na+-dependent protein export, strongly suggesting SecDF functions via cation-coupled protein translocation.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciEcoCyc:SECF.
ECOL316407:JW0399-MONOMER.

Protein family/group databases

TCDBi2.A.6.4.1. the resistance-nodulation-cell division (rnd) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein translocase subunit SecF
Gene namesi
Name:secF
Ordered Locus Names:b0409, JW0399
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10940. secF.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2222CytoplasmicSequence analysisAdd
BLAST
Transmembranei23 – 4321HelicalSequence analysisAdd
BLAST
Topological domaini44 – 14299PeriplasmicSequence analysisAdd
BLAST
Transmembranei143 – 16321HelicalSequence analysisAdd
BLAST
Topological domaini164 – 1707CytoplasmicSequence analysis
Transmembranei171 – 19121HelicalSequence analysisAdd
BLAST
Topological domaini192 – 1965PeriplasmicSequence analysis
Transmembranei197 – 21721HelicalSequence analysisAdd
BLAST
Topological domaini218 – 24730CytoplasmicSequence analysisAdd
BLAST
Transmembranei248 – 27023HelicalSequence analysisAdd
BLAST
Topological domaini271 – 28010PeriplasmicSequence analysis
Transmembranei281 – 30121HelicalSequence analysisAdd
BLAST
Topological domaini302 – 32322CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Disruption of both secD and SecF confers cold-sensitive growth (strain secD1(Cs)).1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi213 – 2131D → M: Abolishes protein translocation. 1 Publication
Mutagenesisi247 – 2471R → M: Abolishes protein translocation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 323323Protein translocase subunit SecFPRO_0000095976Add
BLAST

Proteomic databases

PaxDbiP0AG93.
PRIDEiP0AG93.

Interactioni

Subunit structurei

Part of the prokaryotic protein translocation apparatus which is comprised of SecA, SecB, SecD, SecE, SecF, SecG and SecY.

Protein-protein interaction databases

BioGridi4263101. 280 interactions.
STRINGi511145.b0409.

Structurei

3D structure databases

ProteinModelPortaliP0AG93.
SMRiP0AG93. Positions 44-297.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SecD/SecF family. SecF subfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107RAB. Bacteria.
COG0341. LUCA.
HOGENOMiHOG000245914.
InParanoidiP0AG93.
KOiK03074.
OMAiMRWDNVA.
OrthoDBiEOG676Z5R.
PhylomeDBiP0AG93.

Family and domain databases

HAMAPiMF_01464_B. SecF_B.
InterProiIPR022813. SecD/SecF_arch_bac.
IPR022645. SecD/SecF_bac.
IPR022646. SecD/SecF_CS.
IPR005665. SecF_bac.
[Graphical view]
PfamiPF07549. Sec_GG. 1 hit.
PF02355. SecD_SecF. 1 hit.
[Graphical view]
PRINTSiPR01755. SECFTRNLCASE.
TIGRFAMsiTIGR00916. 2A0604s01. 1 hit.
TIGR00966. 3a0501s07. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AG93-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQEYTVEQL NHGRKVYDFM RWDYWAFGIS GLLLIAAIVI MGVRGFNWGL
60 70 80 90 100
DFTGGTVIEI TLEKPAEIDV MRDALQKAGF EEPMLQNFGS SHDIMVRMPP
110 120 130 140 150
AEGETGGQVL GSQVLKVINE STNQNAAVKR IEFVGPSVGA DLAQTGAMAL
160 170 180 190 200
MAALLSILVY VGFRFEWRLA AGVVIALAHD VIITLGILSL FHIEIDLTIV
210 220 230 240 250
ASLMSVIGYS LNDSIVVSDR IRENFRKIRR GTPYEIFNVS LTQTLHRTLI
260 270 280 290 300
TSGTTLMVIL MLYLFGGPVL EGFSLTMLIG VSIGTASSIY VASALALKLG
310 320
MKREHMLQQK VEKEGADQPS ILP
Length:323
Mass (Da):35,382
Last modified:December 20, 2005 - v1
Checksum:i9662196085448643
GO

Sequence cautioni

The sequence AAB40165.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56175 Genomic DNA. Translation: CAA39635.1.
U82664 Genomic DNA. Translation: AAB40165.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73512.1.
AP009048 Genomic DNA. Translation: BAE76189.1.
PIRiJQ0697.
RefSeqiNP_414943.1. NC_000913.3.
WP_000046637.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73512; AAC73512; b0409.
BAE76189; BAE76189; BAE76189.
GeneIDi949120.
KEGGiecj:JW0399.
eco:b0409.
PATRICi32115967. VBIEscCol129921_0425.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56175 Genomic DNA. Translation: CAA39635.1.
U82664 Genomic DNA. Translation: AAB40165.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73512.1.
AP009048 Genomic DNA. Translation: BAE76189.1.
PIRiJQ0697.
RefSeqiNP_414943.1. NC_000913.3.
WP_000046637.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AG93.
SMRiP0AG93. Positions 44-297.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263101. 280 interactions.
STRINGi511145.b0409.

Protein family/group databases

TCDBi2.A.6.4.1. the resistance-nodulation-cell division (rnd) superfamily.

Proteomic databases

PaxDbiP0AG93.
PRIDEiP0AG93.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73512; AAC73512; b0409.
BAE76189; BAE76189; BAE76189.
GeneIDi949120.
KEGGiecj:JW0399.
eco:b0409.
PATRICi32115967. VBIEscCol129921_0425.

Organism-specific databases

EchoBASEiEB0933.
EcoGeneiEG10940. secF.

Phylogenomic databases

eggNOGiENOG4107RAB. Bacteria.
COG0341. LUCA.
HOGENOMiHOG000245914.
InParanoidiP0AG93.
KOiK03074.
OMAiMRWDNVA.
OrthoDBiEOG676Z5R.
PhylomeDBiP0AG93.

Enzyme and pathway databases

BioCyciEcoCyc:SECF.
ECOL316407:JW0399-MONOMER.

Miscellaneous databases

PROiP0AG93.

Family and domain databases

HAMAPiMF_01464_B. SecF_B.
InterProiIPR022813. SecD/SecF_arch_bac.
IPR022645. SecD/SecF_bac.
IPR022646. SecD/SecF_CS.
IPR005665. SecF_bac.
[Graphical view]
PfamiPF07549. Sec_GG. 1 hit.
PF02355. SecD_SecF. 1 hit.
[Graphical view]
PRINTSiPR01755. SECFTRNLCASE.
TIGRFAMsiTIGR00916. 2A0604s01. 1 hit.
TIGR00966. 3a0501s07. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The secD locus of E.coli codes for two membrane proteins required for protein export."
    Gardel C., Johnson K., Jacq A., Beckwith J.
    EMBO J. 9:3209-3216(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE.
    Strain: K12.
  2. Erratum
    Gardel C., Johnson K., Jacq A., Beckwith J.
    EMBO J. 9:4205-4206(1990) [PubMed] [Europe PMC] [Abstract]
  3. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.
  7. "Structure and function of a membrane component SecDF that enhances protein export."
    Tsukazaki T., Mori H., Echizen Y., Ishitani R., Fukai S., Tanaka T., Perederina A., Vassylyev D.G., Kohno T., Maturana A.D., Ito K., Nureki O.
    Nature 474:235-238(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN TRANSLOCATION, MUTAGENESIS OF ASP-213 AND ARG-247.

Entry informationi

Entry nameiSECF_ECOLI
AccessioniPrimary (citable) accession number: P0AG93
Secondary accession number(s): P19674, P77113, Q2MC17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: January 20, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.