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Protein

Protein translocase subunit SecF

Gene

secF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA. The large periplasmic domain is thought to have a base and head domain joined by a hinge; movement of the hinge may be coupled to both proton transport and protein export, with the head domain capturing substrate, and a conformational change preventing backward movement and driving forward movement. Expression of V.alginolyticus SecD and SecF in E.coli confers Na+-dependent protein export, strongly suggesting SecDF functions via cation-coupled protein translocation.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciEcoCyc:SECF

Protein family/group databases

TCDBi2.A.6.4.1 the resistance-nodulation-cell division (rnd) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Protein translocase subunit SecF
Alternative name(s):
Sec translocon accessory complex subunit SecF
Gene namesi
Name:secF
Ordered Locus Names:b0409, JW0399
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10940 secF

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 22CytoplasmicSequence analysisAdd BLAST22
Transmembranei23 – 43HelicalSequence analysisAdd BLAST21
Topological domaini44 – 142PeriplasmicSequence analysisAdd BLAST99
Transmembranei143 – 163HelicalSequence analysisAdd BLAST21
Topological domaini164 – 170CytoplasmicSequence analysis7
Transmembranei171 – 191HelicalSequence analysisAdd BLAST21
Topological domaini192 – 196PeriplasmicSequence analysis5
Transmembranei197 – 217HelicalSequence analysisAdd BLAST21
Topological domaini218 – 247CytoplasmicSequence analysisAdd BLAST30
Transmembranei248 – 270HelicalSequence analysisAdd BLAST23
Topological domaini271 – 280PeriplasmicSequence analysis10
Transmembranei281 – 301HelicalSequence analysisAdd BLAST21
Topological domaini302 – 323CytoplasmicSequence analysisAdd BLAST22

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • intracellular Source: GOC
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Disruption of both secD and SecF confers cold-sensitive growth (strain secD1(Cs)).1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi213D → M: Abolishes protein translocation. 1 Publication1
Mutagenesisi247R → M: Abolishes protein translocation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000959761 – 323Protein translocase subunit SecFAdd BLAST323

Proteomic databases

PaxDbiP0AG93
PRIDEiP0AG93

Interactioni

Subunit structurei

Part of the prokaryotic protein translocation apparatus which is comprised of SecA, SecB, SecD, SecE, SecF, SecG and SecY. The SecDF-YidC-YajC translocase forms a supercomplex with SecYEG, called the holo-translocon (HTL) (PubMed:27435098). The stoichiometry of the super complex may be SecYEG:YidC:SecDF 4:3:1, YajC is in the reconstituted complex (with SecDF) but as no antibody is available it could not be quantified (PubMed:27435098).1 Publication

Protein-protein interaction databases

BioGridi4263101, 312 interactors
STRINGi316385.ECDH10B_0365

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5MG3electron microscopy14.00F1-323[»]
ProteinModelPortaliP0AG93
SMRiP0AG93
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SecD/SecF family. SecF subfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107RAB Bacteria
COG0341 LUCA
HOGENOMiHOG000245914
InParanoidiP0AG93
KOiK03074
OMAiVNQTLMR
PhylomeDBiP0AG93

Family and domain databases

HAMAPiMF_01464_B SecF_B, 1 hit
InterProiView protein in InterPro
IPR022813 SecD/SecF_arch_bac
IPR022645 SecD/SecF_bac
IPR022646 SecD/SecF_CS
IPR005665 SecF_bac
PfamiView protein in Pfam
PF07549 Sec_GG, 1 hit
PF02355 SecD_SecF, 1 hit
PRINTSiPR01755 SECFTRNLCASE
TIGRFAMsiTIGR00916 2A0604s01, 1 hit
TIGR00966 3a0501s07, 1 hit

Sequencei

Sequence statusi: Complete.

P0AG93-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQEYTVEQL NHGRKVYDFM RWDYWAFGIS GLLLIAAIVI MGVRGFNWGL
60 70 80 90 100
DFTGGTVIEI TLEKPAEIDV MRDALQKAGF EEPMLQNFGS SHDIMVRMPP
110 120 130 140 150
AEGETGGQVL GSQVLKVINE STNQNAAVKR IEFVGPSVGA DLAQTGAMAL
160 170 180 190 200
MAALLSILVY VGFRFEWRLA AGVVIALAHD VIITLGILSL FHIEIDLTIV
210 220 230 240 250
ASLMSVIGYS LNDSIVVSDR IRENFRKIRR GTPYEIFNVS LTQTLHRTLI
260 270 280 290 300
TSGTTLMVIL MLYLFGGPVL EGFSLTMLIG VSIGTASSIY VASALALKLG
310 320
MKREHMLQQK VEKEGADQPS ILP
Length:323
Mass (Da):35,382
Last modified:December 20, 2005 - v1
Checksum:i9662196085448643
GO

Sequence cautioni

The sequence AAB40165 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56175 Genomic DNA Translation: CAA39635.1
U82664 Genomic DNA Translation: AAB40165.1 Different initiation.
U00096 Genomic DNA Translation: AAC73512.1
AP009048 Genomic DNA Translation: BAE76189.1
PIRiJQ0697
RefSeqiNP_414943.1, NC_000913.3
WP_000046637.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73512; AAC73512; b0409
BAE76189; BAE76189; BAE76189
GeneIDi949120
KEGGiecj:JW0399
eco:b0409
PATRICifig|1411691.4.peg.1868

Similar proteinsi

Entry informationi

Entry nameiSECF_ECOLI
AccessioniPrimary (citable) accession number: P0AG93
Secondary accession number(s): P19674, P77113, Q2MC17
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: April 25, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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