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P0AG86 (SECB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Protein-export protein SecB
Gene names
Name:secB
Ordered Locus Names:b3609, JW3584
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length155 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

One of the proteins required for the normal export of some preproteins out of the cell cytoplasm. It is a molecular chaperone that binds to a subset of precursor proteins, maintaining them in a translocation-competent state. It also specifically binds to its receptor SecA. Its substrates include AmpC, DegP, FhuA, FkpA, GBP, LamB, MalE (MBP), OmpA, OmpF, OmpT, OmpX, OppA, PhoE, TolB, TolC, YbgF, YcgK, YgiW and YncE. HAMAP-Rule MF_00821

Subunit structure

Homotetramer, a dimer of dimers. One homotetramer interacts with 1 SecA dimer.

Subcellular location

Cytoplasm HAMAP-Rule MF_00821.

Disruption phenotype

Growth continues, but export of its substrates is blocked. Expression of chaperones DnaK, GroEL/ES, ClpB, and HslU increases. Ref.7

Sequence similarities

Belongs to the SecB family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

secAP104084EBI-555877,EBI-543213

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 155155Protein-export protein SecB HAMAP-Rule MF_00821
PRO_0000055370

Secondary structure

................. 155
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0AG86 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: 00514C5F393A643D

FASTA15517,277
        10         20         30         40         50         60 
MSEQNNTEMT FQIQRIYTKD ISFEAPNAPH VFQKDWQPEV KLDLDTASSQ LADDVYEVVL 

        70         80         90        100        110        120 
RVTVTASLGE ETAFLCEVQQ GGIFSIAGIE GTQMAHCLGA YCPNILFPYA RECITSMVSR 

       130        140        150 
GTFPQLNLAP VNFDALFMNY LQQQAGEGTE EHQDA 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of the Escherichia coli protein-export gene secB."
Kumamoto C.A., Nault A.K.
Gene 75:167-175(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Escherichia coli proteome analysis using the gene-protein database."
VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY 2D-GEL.
[6]"Crystal structure of SecB from Escherichia coli."
Dekker C., de Kruijff B., Gros P.
J. Struct. Biol. 144:313-319(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS).
[7]"Defining the role of the Escherichia coli chaperone SecB using comparative proteomics."
Baars L., Ytterberg A.J., Drew D., Wagner S., Thilo C., van Wijk K.J., de Gier J.W.
J. Biol. Chem. 281:10024-10034(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBSTRATES, DISRUPTION PHENOTYPE.
Strain: K12 / MC4100.
[8]"Bacterial protein secretion through the translocase nanomachine."
Papanikou E., Karamanou S., Economou A.
Nat. Rev. Microbiol. 5:839-851(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW OF PROTEIN SECRETION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M24489 Genomic DNA. Translation: AAA83907.1.
U00039 Genomic DNA. Translation: AAB18586.1.
U00096 Genomic DNA. Translation: AAC76633.1.
AP009048 Genomic DNA. Translation: BAE77683.1.
PIRVXECS. JS0126.
RefSeqNP_418066.1. NC_000913.2.
YP_491824.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1QYNX-ray2.35A/B/C/D1-153[»]
ProteinModelPortalP0AG86.
SMRP0AG86. Positions 9-144.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-47924N.
IntActP0AG86. 14 interactions.
MINTMINT-1248893.
STRING511145.b3609.

PTM databases

PhosSiteP0810436.

Proteomic databases

PaxDbP0AG86.
PRIDEP0AG86.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76633; AAC76633; b3609.
BAE77683; BAE77683; BAE77683.
GeneID12932409.
948123.
KEGGecj:Y75_p3565.
eco:b3609.
PATRIC32122703. VBIEscCol129921_3728.

Organism-specific databases

EchoBASEEB0930.
EcoGeneEG10937. secB.

Phylogenomic databases

eggNOGCOG1952.
HOGENOMHOG000218192.
KOK03071.
OMAGFPPLMI.
ProtClustDBPRK05751.

Enzyme and pathway databases

BioCycEcoCyc:SECB.
ECOL316407:JW3584-MONOMER.

Gene expression databases

GenevestigatorP0AG86.

Family and domain databases

Gene3D3.10.420.10. 1 hit.
HAMAPMF_00821. SecB.
InterProIPR003708. SecB.
[Graphical view]
PfamPF02556. SecB. 1 hit.
[Graphical view]
PRINTSPR01594. SECBCHAPRONE.
SUPFAMSSF54611. SecB. 1 hit.
TIGRFAMsTIGR00809. secB. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP0AG86.

Entry information

Entry nameSECB_ECOLI
AccessionPrimary (citable) accession number: P0AG86
Secondary accession number(s): P15040, Q2M7S3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families