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P0AG86

- SECB_ECOLI

UniProt

P0AG86 - SECB_ECOLI

Protein

Protein-export protein SecB

Gene

secB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    One of the proteins required for the normal export of some preproteins out of the cell cytoplasm. It is a molecular chaperone that binds to a subset of precursor proteins, maintaining them in a translocation-competent state. It also specifically binds to its receptor SecA. Its substrates include AmpC, DegP, FhuA, FkpA, GBP, LamB, MalE (MBP), OmpA, OmpF, OmpT, OmpX, OppA, PhoE, TolB, TolC, YbgF, YcgK, YgiW and YncE.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. unfolded protein binding Source: EcoCyc

    GO - Biological processi

    1. protein folding Source: UniProtKB-HAMAP
    2. protein targeting Source: EcoliWiki
    3. protein tetramerization Source: EcoCyc
    4. protein transport Source: EcoliWiki
    5. protein transport by the Sec complex Source: EcoliWiki

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    Protein transport, Translocation, Transport

    Enzyme and pathway databases

    BioCyciEcoCyc:SECB.
    ECOL316407:JW3584-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein-export protein SecB
    Gene namesi
    Name:secB
    Ordered Locus Names:b3609, JW3584
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10937. secB.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: EcoCyc

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Growth continues, but export of its substrates is blocked. Expression of chaperones DnaK, GroEL/ES, ClpB, and HslU increases.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 155155Protein-export protein SecBPRO_0000055370Add
    BLAST

    Proteomic databases

    PaxDbiP0AG86.
    PRIDEiP0AG86.

    PTM databases

    PhosSiteiP0810436.

    Expressioni

    Gene expression databases

    GenevestigatoriP0AG86.

    Interactioni

    Subunit structurei

    Homotetramer, a dimer of dimers. One homotetramer interacts with 1 SecA dimer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    secAP104084EBI-555877,EBI-543213

    Protein-protein interaction databases

    DIPiDIP-47924N.
    IntActiP0AG86. 14 interactions.
    MINTiMINT-1248893.
    STRINGi511145.b3609.

    Structurei

    Secondary structure

    1
    155
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi10 – 2415
    Helixi28 – 314
    Beta strandi39 – 5214
    Beta strandi55 – 6814
    Beta strandi71 – 8818
    Helixi91 – 999
    Helixi101 – 12121
    Helixi133 – 1419

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1QYNX-ray2.35A/B/C/D1-153[»]
    ProteinModelPortaliP0AG86.
    SMRiP0AG86. Positions 9-144.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0AG86.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the SecB family.Curated

    Phylogenomic databases

    eggNOGiCOG1952.
    HOGENOMiHOG000218192.
    KOiK03071.
    OMAiGSFPQLN.
    OrthoDBiEOG6DVJZZ.
    PhylomeDBiP0AG86.

    Family and domain databases

    Gene3Di3.10.420.10. 1 hit.
    HAMAPiMF_00821. SecB.
    InterProiIPR003708. SecB.
    [Graphical view]
    PfamiPF02556. SecB. 1 hit.
    [Graphical view]
    PRINTSiPR01594. SECBCHAPRONE.
    SUPFAMiSSF54611. SSF54611. 1 hit.
    TIGRFAMsiTIGR00809. secB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0AG86-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEQNNTEMT FQIQRIYTKD ISFEAPNAPH VFQKDWQPEV KLDLDTASSQ    50
    LADDVYEVVL RVTVTASLGE ETAFLCEVQQ GGIFSIAGIE GTQMAHCLGA 100
    YCPNILFPYA RECITSMVSR GTFPQLNLAP VNFDALFMNY LQQQAGEGTE 150
    EHQDA 155
    Length:155
    Mass (Da):17,277
    Last modified:December 20, 2005 - v1
    Checksum:i00514C5F393A643D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M24489 Genomic DNA. Translation: AAA83907.1.
    U00039 Genomic DNA. Translation: AAB18586.1.
    U00096 Genomic DNA. Translation: AAC76633.1.
    AP009048 Genomic DNA. Translation: BAE77683.1.
    PIRiJS0126. VXECS.
    RefSeqiNP_418066.1. NC_000913.3.
    YP_491824.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76633; AAC76633; b3609.
    BAE77683; BAE77683; BAE77683.
    GeneIDi12932409.
    948123.
    KEGGiecj:Y75_p3565.
    eco:b3609.
    PATRICi32122703. VBIEscCol129921_3728.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M24489 Genomic DNA. Translation: AAA83907.1 .
    U00039 Genomic DNA. Translation: AAB18586.1 .
    U00096 Genomic DNA. Translation: AAC76633.1 .
    AP009048 Genomic DNA. Translation: BAE77683.1 .
    PIRi JS0126. VXECS.
    RefSeqi NP_418066.1. NC_000913.3.
    YP_491824.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1QYN X-ray 2.35 A/B/C/D 1-153 [» ]
    ProteinModelPortali P0AG86.
    SMRi P0AG86. Positions 9-144.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-47924N.
    IntActi P0AG86. 14 interactions.
    MINTi MINT-1248893.
    STRINGi 511145.b3609.

    PTM databases

    PhosSitei P0810436.

    Proteomic databases

    PaxDbi P0AG86.
    PRIDEi P0AG86.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76633 ; AAC76633 ; b3609 .
    BAE77683 ; BAE77683 ; BAE77683 .
    GeneIDi 12932409.
    948123.
    KEGGi ecj:Y75_p3565.
    eco:b3609.
    PATRICi 32122703. VBIEscCol129921_3728.

    Organism-specific databases

    EchoBASEi EB0930.
    EcoGenei EG10937. secB.

    Phylogenomic databases

    eggNOGi COG1952.
    HOGENOMi HOG000218192.
    KOi K03071.
    OMAi GSFPQLN.
    OrthoDBi EOG6DVJZZ.
    PhylomeDBi P0AG86.

    Enzyme and pathway databases

    BioCyci EcoCyc:SECB.
    ECOL316407:JW3584-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0AG86.
    PROi P0AG86.

    Gene expression databases

    Genevestigatori P0AG86.

    Family and domain databases

    Gene3Di 3.10.420.10. 1 hit.
    HAMAPi MF_00821. SecB.
    InterProi IPR003708. SecB.
    [Graphical view ]
    Pfami PF02556. SecB. 1 hit.
    [Graphical view ]
    PRINTSi PR01594. SECBCHAPRONE.
    SUPFAMi SSF54611. SSF54611. 1 hit.
    TIGRFAMsi TIGR00809. secB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the Escherichia coli protein-export gene secB."
      Kumamoto C.A., Nault A.K.
      Gene 75:167-175(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
      Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
      Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Escherichia coli proteome analysis using the gene-protein database."
      VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
      Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY 2D-GEL.
    6. "Crystal structure of SecB from Escherichia coli."
      Dekker C., de Kruijff B., Gros P.
      J. Struct. Biol. 144:313-319(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS).
    7. "Defining the role of the Escherichia coli chaperone SecB using comparative proteomics."
      Baars L., Ytterberg A.J., Drew D., Wagner S., Thilo C., van Wijk K.J., de Gier J.W.
      J. Biol. Chem. 281:10024-10034(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBSTRATES, DISRUPTION PHENOTYPE.
      Strain: K12 / MC4100.
    8. "Bacterial protein secretion through the translocase nanomachine."
      Papanikou E., Karamanou S., Economou A.
      Nat. Rev. Microbiol. 5:839-851(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW OF PROTEIN SECRETION.

    Entry informationi

    Entry nameiSECB_ECOLI
    AccessioniPrimary (citable) accession number: P0AG86
    Secondary accession number(s): P15040, Q2M7S3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3