Protein-export protein SecB - Escherichia coli (strain K12)

Contribute

Send feedback

Read comments (?) or add your own

P0AG86 (SECB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 59. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein-export protein SecB

Gene names

Name:	secB
Ordered Locus Names:	b3609, JW3584

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	155 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	One of the proteins required for the normal export of some preproteins out of the cell cytoplasm. It is a molecular chaperone that binds to a subset of precursor proteins, maintaining them in a translocation-competent state. It also specifically binds to its receptor SecA. Its substrates include AmpC, DegP, FhuA, FkpA, GBP, LamB, MalE (MBP), OmpA, OmpF, OmpT, OmpX, OppA, PhoE, TolB, TolC, YbgF, YcgK, YgiW and YncE. HAMAP MF_00821
Subunit structure	Homotetramer, a dimer of dimers. One homotetramer interacts with 1 SecA dimer.
Subcellular location	Cytoplasm HAMAP MF_00821.
Disruption phenotype	Growth continues, but export of its substrates is blocked. Expression of chaperones DnaK, GroEL/ES, ClpB, and HslU increases. Ref.6
Sequence similarities	Belongs to the secB family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein targeting Inferred from mutant phenotype. Source: EcoliWiki protein tetramerization Inferred from electronic annotation. Source: InterPro protein transport by the Sec complex Inferred from mutant phenotype. Source: EcoliWiki
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	unfolded protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 155

155

Protein-export protein SecB HAMAP MF_00821

PRO_0000055370

Secondary structure

155

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0AG86 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: 00514C5F393A643D
Show »

FASTA

155

17,277

        10         20         30         40         50         60 
MSEQNNTEMT FQIQRIYTKD ISFEAPNAPH VFQKDWQPEV KLDLDTASSQ LADDVYEVVL 

        70         80         90        100        110        120 
RVTVTASLGE ETAFLCEVQQ GGIFSIAGIE GTQMAHCLGA YCPNILFPYA RECITSMVSR 

       130        140        150 
GTFPQLNLAP VNFDALFMNY LQQQAGEGTE EHQDA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the Escherichia coli protein-export gene secB." Kumamoto C.A., Nault A.K. Gene 75:167-175(1989) [PubMed: 2656409] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Crystal structure of SecB from Escherichia coli." Dekker C., de Kruijff B., Gros P. J. Struct. Biol. 144:313-319(2003) [PubMed: 14643199] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS).
[6]	"Defining the role of the Escherichia coli chaperone SecB using comparative proteomics." Baars L., Ytterberg A.J., Drew D., Wagner S., Thilo C., van Wijk K.J., de Gier J.W. J. Biol. Chem. 281:10024-10034(2006) [PubMed: 16352602] [Abstract] Cited for: SUBSTRATES, DISRUPTION PHENOTYPE. Strain: K12 / MC4100.
[7]	"Bacterial protein secretion through the translocase nanomachine." Papanikou E., Karamanou S., Economou A. Nat. Rev. Microbiol. 5:839-851(2007) [PubMed: 17938627] [Abstract] Cited for: REVIEW OF PROTEIN SECRETION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M24489 Genomic DNA. Translation: AAA83907.1.
U00039 Genomic DNA. Translation: AAB18586.1.
U00096 Genomic DNA. Translation: AAC76633.1.
AP009048 Genomic DNA. Translation: BAE77683.1.

PIR

VXECS. JS0126.

RefSeq

NP_418066.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1QYN	X-ray	2.35	A/B/C/D	1-153	[»]

ProteinModelPortal

P0AG86.

SMR

P0AG86. Positions 9-144.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-47924N.

IntAct

P0AG86. 14 interactions.

MINT

MINT-1248893.

PTM databases

PhosSite

P0AG86.

2D gel databases

ECO2DBASE

A015.6. 6TH EDITION.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000000132; EBESCP00000000132; EBESCG00000000103.
EBESCT00000016245; EBESCP00000015536; EBESCG00000015305.

GeneID

948123.

GenomeReviews

Gene locus JW3584 in contig AP009048_GR.
Gene locus b3609 in contig U00096_GR.

KEGG

ecj:JW3584.
eco:b3609.

PATRIC

32122703. VBIEscCol129921_3728.

Organism-specific databases

EchoBASE

EB0930.

EcoGene

EG10937. secB.

Phylogenomic databases

eggNOG

COG1952.

GeneTree

EBGT00050000010317.

HOGENOM

HBG294212.

OMA

GFPPLMI.

PhylomeDB

P0AG86.

ProtClustDB

PRK05751.

Enzyme and pathway databases

BioCyc

EcoCyc:SECB.

Gene expression databases

Genevestigator

P0AG86.

Family and domain databases

HAMAP

MF_00821. SecB.
[Tree]

InterPro

IPR003708. SecB.
[Graphical view]

Gene3D

G3DSA:3.10.420.10. SecB. 1 hit.

K03071.

Pfam

PF02556. SecB. 1 hit.
[Graphical view]

PRINTS

PR01594. SECBCHAPRONE.

SUPFAM

SSF54611. SecB. 1 hit.

TIGRFAMs

TIGR00809. SecB. 1 hit.

ProtoNet

Search...

Other

BindingDB

P0AG86.

Entry information

Entry name

SECB_ECOLI

Accession

Primary (citable) accession number: P0AG86
Secondary accession number(s): P15040, Q2M7S3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

2D gel databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents