P0AG86 (SECB_ECOLI) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 59.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Protein-export protein SecB | ||||
| Gene names |
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| Organism | Escherichia coli (strain K12) | ||||
| Taxonomic identifier | 83333 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 155 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | One of the proteins required for the normal export of some preproteins out of the cell cytoplasm. It is a molecular chaperone that binds to a subset of precursor proteins, maintaining them in a translocation-competent state. It also specifically binds to its receptor SecA. Its substrates include AmpC, DegP, FhuA, FkpA, GBP, LamB, MalE (MBP), OmpA, OmpF, OmpT, OmpX, OppA, PhoE, TolB, TolC, YbgF, YcgK, YgiW and YncE. HAMAP MF_00821 |
| Subunit structure | Homotetramer, a dimer of dimers. One homotetramer interacts with 1 SecA dimer. |
| Subcellular location | |
| Disruption phenotype | Growth continues, but export of its substrates is blocked. Expression of chaperones DnaK, GroEL/ES, ClpB, and HslU increases. Ref.6 |
| Sequence similarities | Belongs to the secB family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Protein transport Translocation Transport |
| Cellular component | Cytoplasm |
| Molecular function | Chaperone |
| Technical term | 3D-structure Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | protein targeting Inferred from mutant phenotype. Source: EcoliWiki protein tetramerizationInferred from electronic annotation. Source: InterPro protein transport by the Sec complexInferred from mutant phenotype. Source: EcoliWiki |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | unfolded protein binding Inferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||||||||||||||
Molecule processing | |||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 155 | 155 | Protein-export protein SecB HAMAP MF_00821 | PRO_0000055370 | |||||||||||||||||||||
Secondary structure | |||||||||||||||||||||||||
Helix Strand Turn | |||||||||||||||||||||||||
| Beta strand | 10 – 24 | 15 | |||||||||||||||||||||||
| Helix | 28 – 31 | 4 | |||||||||||||||||||||||
| Beta strand | 39 – 52 | 14 | |||||||||||||||||||||||
| Beta strand | 55 – 68 | 14 | |||||||||||||||||||||||
| Beta strand | 71 – 88 | 18 | |||||||||||||||||||||||
| Helix | 91 – 99 | 9 | |||||||||||||||||||||||
| Helix | 101 – 121 | 21 | |||||||||||||||||||||||
| Helix | 133 – 141 | 9 | |||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Characterization of the Escherichia coli protein-export gene secB." Kumamoto C.A., Nault A.K. Gene 75:167-175(1989) [PubMed: 2656409] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12. |
| [2] | "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [3] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [4] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [5] | "Crystal structure of SecB from Escherichia coli." Dekker C., de Kruijff B., Gros P. J. Struct. Biol. 144:313-319(2003) [PubMed: 14643199] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS). |
| [6] | "Defining the role of the Escherichia coli chaperone SecB using comparative proteomics." Baars L., Ytterberg A.J., Drew D., Wagner S., Thilo C., van Wijk K.J., de Gier J.W. J. Biol. Chem. 281:10024-10034(2006) [PubMed: 16352602] [Abstract] Cited for: SUBSTRATES, DISRUPTION PHENOTYPE. Strain: K12 / MC4100. |
| [7] | "Bacterial protein secretion through the translocase nanomachine." Papanikou E., Karamanou S., Economou A. Nat. Rev. Microbiol. 5:839-851(2007) [PubMed: 17938627] [Abstract] Cited for: REVIEW OF PROTEIN SECRETION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | M24489 Genomic DNA. Translation: AAA83907.1. U00039 Genomic DNA. Translation: AAB18586.1. U00096 Genomic DNA. Translation: AAC76633.1. AP009048 Genomic DNA. Translation: BAE77683.1. | ||||||||||||
| PIR | VXECS. JS0126. | ||||||||||||
| RefSeq | NP_418066.1. NC_000913.2. | ||||||||||||
3D structure databases | |||||||||||||
| PDBe RCSB PDB PDBj |
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| ProteinModelPortal | P0AG86. | ||||||||||||
| SMR | P0AG86. Positions 9-144. | ||||||||||||
| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| DIP | DIP-47924N. | ||||||||||||
| IntAct | P0AG86. 14 interactions. | ||||||||||||
| MINT | MINT-1248893. | ||||||||||||
PTM databases | |||||||||||||
| PhosSite | P0AG86. | ||||||||||||
2D gel databases | |||||||||||||
| ECO2DBASE | A015.6. 6TH EDITION. | ||||||||||||
Protocols and materials databases | |||||||||||||
| StructuralBiologyKnowledgebase | Search... | ||||||||||||
Genome annotation databases | |||||||||||||
| EnsemblBacteria | EBESCT00000000132; EBESCP00000000132; EBESCG00000000103. EBESCT00000016245; EBESCP00000015536; EBESCG00000015305. | ||||||||||||
| GeneID | 948123. | ||||||||||||
| GenomeReviews | Gene locus JW3584 in contig AP009048_GR. Gene locus b3609 in contig U00096_GR. | ||||||||||||
| KEGG | ecj:JW3584. eco:b3609. | ||||||||||||
| PATRIC | 32122703. VBIEscCol129921_3728. | ||||||||||||
Organism-specific databases | |||||||||||||
| EchoBASE | EB0930. | ||||||||||||
| EcoGene | EG10937. secB. | ||||||||||||
Phylogenomic databases | |||||||||||||
| eggNOG | COG1952. | ||||||||||||
| GeneTree | EBGT00050000010317. | ||||||||||||
| HOGENOM | HBG294212. | ||||||||||||
| OMA | GFPPLMI. | ||||||||||||
| PhylomeDB | P0AG86. | ||||||||||||
| ProtClustDB | PRK05751. | ||||||||||||
Enzyme and pathway databases | |||||||||||||
| BioCyc | EcoCyc:SECB. | ||||||||||||
Gene expression databases | |||||||||||||
| Genevestigator | P0AG86. | ||||||||||||
Family and domain databases | |||||||||||||
| HAMAP | MF_00821. SecB. [Tree] | ||||||||||||
| InterPro | IPR003708. SecB. [Graphical view] | ||||||||||||
| Gene3D | G3DSA:3.10.420.10. SecB. 1 hit. | ||||||||||||
| KO | K03071. | ||||||||||||
| Pfam | PF02556. SecB. 1 hit. [Graphical view] | ||||||||||||
| PRINTS | PR01594. SECBCHAPRONE. | ||||||||||||
| SUPFAM | SSF54611. SecB. 1 hit. | ||||||||||||
| TIGRFAMs | TIGR00809. SecB. 1 hit. | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other | |||||||||||||
| BindingDB | P0AG86. | ||||||||||||
Entry information
| Entry name | SECB_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P0AG86 Secondary accession number(s): P15040, Q2M7S3 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with