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Protein

Phosphate-binding protein PstS

Gene

pstS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex PstSACB involved in phosphate import.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei63PhosphateBy similarity1
Binding sitei81PhosphateBy similarity1

GO - Molecular functioni

  • phosphate ion binding Source: EcoCyc

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • phosphate ion transmembrane transport Source: InterPro
  • phosphate ion transport Source: EcoCyc
  • regulation of phosphatase activity Source: EcoCyc
  • response to radiation Source: EcoCyc

Keywordsi

Biological processPhosphate transport, Stress response, Transport

Enzyme and pathway databases

BioCyciEcoCyc:PSTS-MONOMER
MetaCyc:PSTS-MONOMER

Protein family/group databases

TCDBi3.A.1.7.1 the atp-binding cassette (abc) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphate-binding protein PstS
Short name:
PBP
Gene namesi
Name:pstS
Synonyms:phoS
Ordered Locus Names:b3728, JW3706
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10734 pstS

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Chemistry databases

DrugBankiDB02831 Dihydrogenphosphate Ion
DB02799 N-[2-(1-Maleimidyl)Ethyl]-7-Diethylaminocoumarin-3-Carboxamide

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 251 PublicationAdd BLAST25
ChainiPRO_000003184826 – 346Phosphate-binding protein PstSAdd BLAST321

Proteomic databases

EPDiP0AG82
PaxDbiP0AG82
PRIDEiP0AG82

2D gel databases

SWISS-2DPAGEiP0AG82

Expressioni

Inductioni

By phosphate deprivation. Positively regulated by PhoB and negatively regulated by PhoR.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).Curated

Protein-protein interaction databases

BioGridi4262139, 97 interactors
DIPiDIP-48241N
IntActiP0AG82, 2 interactors
STRINGi316385.ECDH10B_3915

Structurei

Secondary structure

1346
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi28 – 33Combined sources6
Beta strandi35 – 37Combined sources3
Helixi38 – 52Combined sources15
Beta strandi55 – 60Combined sources6
Helixi63 – 71Combined sources9
Beta strandi75 – 82Combined sources8
Helixi86 – 92Combined sources7
Beta strandi94 – 108Combined sources15
Turni115 – 117Combined sources3
Helixi122 – 129Combined sources8
Helixi139 – 144Combined sources6
Beta strandi156 – 162Combined sources7
Helixi165 – 177Combined sources13
Helixi179 – 184Combined sources6
Beta strandi188 – 190Combined sources3
Beta strandi197 – 200Combined sources4
Helixi201 – 211Combined sources11
Beta strandi215 – 220Combined sources6
Helixi221 – 225Combined sources5
Turni226 – 228Combined sources3
Beta strandi233 – 235Combined sources3
Helixi246 – 252Combined sources7
Turni253 – 255Combined sources3
Turni258 – 260Combined sources3
Beta strandi271 – 273Combined sources3
Beta strandi276 – 288Combined sources13
Helixi292 – 308Combined sources17
Helixi310 – 315Combined sources6
Helixi323 – 336Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A40X-ray1.70A26-346[»]
1A54X-ray1.60A26-346[»]
1A55X-ray2.40A26-346[»]
1IXGX-ray1.05A26-346[»]
1IXHX-ray0.98A26-346[»]
1IXIX-ray1.89A26-346[»]
1OIBX-ray2.40A/B26-346[»]
1PBPX-ray1.90A26-346[»]
1QUIX-ray1.90A26-346[»]
1QUJX-ray1.90A26-346[»]
1QUKX-ray1.70A26-346[»]
1QULX-ray1.70A26-346[»]
2ABHX-ray1.70A26-346[»]
ProteinModelPortaliP0AG82
SMRiP0AG82
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AG82

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni34 – 36Phosphate bindingBy similarity3
Regioni164 – 166Phosphate bindingBy similarity3

Sequence similaritiesi

Belongs to the PstS family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QMV Bacteria
COG0226 LUCA
HOGENOMiHOG000236794
InParanoidiP0AG82
KOiK02040
OMAiYVEYAYV
PhylomeDBiP0AG82

Family and domain databases

InterProiView protein in InterPro
IPR005673 ABC_phos-bd_PstS
IPR024370 PBP_domain
PfamiView protein in Pfam
PF12849 PBP_like_2, 1 hit
PIRSFiPIRSF002756 PstS, 1 hit
TIGRFAMsiTIGR00975 3a0107s03, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AG82-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVMRTTVAT VVAATLSMSA FSVFAEASLT GAGATFPAPV YAKWADTYQK
60 70 80 90 100
ETGNKVNYQG IGSSGGVKQI IANTVDFGAS DAPLSDEKLA QEGLFQFPTV
110 120 130 140 150
IGGVVLAVNI PGLKSGELVL DGKTLGDIYL GKIKKWDDEA IAKLNPGLKL
160 170 180 190 200
PSQNIAVVRR ADGSGTSFVF TSYLAKVNEE WKNNVGTGST VKWPIGLGGK
210 220 230 240 250
GNDGIAAFVQ RLPGAIGYVE YAYAKQNNLA YTKLISADGK PVSPTEENFA
260 270 280 290 300
NAAKGADWSK TFAQDLTNQK GEDAWPITST TFILIHKDQK KPEQGTEVLK
310 320 330 340
FFDWAYKTGA KQANDLDYAS LPDSVVEQVR AAWKTNIKDS SGKPLY
Length:346
Mass (Da):37,024
Last modified:January 1, 1988 - v1
Checksum:i867DA7199C2C87ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01992 Genomic DNA Translation: AAA24378.1
L10328 Genomic DNA Translation: AAA62079.1
U00096 Genomic DNA Translation: AAC76751.1
AP009048 Genomic DNA Translation: BAE77560.1
PIRiA30277 BYECPR
RefSeqiNP_418184.1, NC_000913.3
WP_000867146.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76751; AAC76751; b3728
BAE77560; BAE77560; BAE77560
GeneIDi948237
KEGGiecj:JW3706
eco:b3728
PATRICifig|1411691.4.peg.2972

Similar proteinsi

Entry informationi

Entry nameiPSTS_ECOLI
AccessioniPrimary (citable) accession number: P0AG82
Secondary accession number(s): P06128, P76744, Q2M846
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: March 28, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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