Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphate-binding protein PstS

Gene

pstS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex PstSACB involved in phosphate import.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei63 – 631PhosphateBy similarity
Binding sitei81 – 811PhosphateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Phosphate transport, Stress response, Transport

Enzyme and pathway databases

BioCyciEcoCyc:PSTS-MONOMER.
ECOL316407:JW3706-MONOMER.
MetaCyc:PSTS-MONOMER.

Protein family/group databases

TCDBi3.A.1.7.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphate-binding protein PstS
Short name:
PBP
Gene namesi
Name:pstS
Synonyms:phoS
Ordered Locus Names:b3728, JW3706
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10734. pstS.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 25251 PublicationAdd
BLAST
Chaini26 – 346321Phosphate-binding protein PstSPRO_0000031848Add
BLAST

Proteomic databases

EPDiP0AG82.
PaxDbiP0AG82.
PRIDEiP0AG82.

2D gel databases

SWISS-2DPAGEP0AG82.

Expressioni

Inductioni

By phosphate deprivation. Positively regulated by PhoB and negatively regulated by PhoR.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).Curated

Protein-protein interaction databases

BioGridi4262139. 6 interactions.
DIPiDIP-48241N.
IntActiP0AG82. 2 interactions.
MINTiMINT-1224444.
STRINGi511145.b3728.

Structurei

Secondary structure

1
346
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi28 – 336Combined sources
Beta strandi35 – 373Combined sources
Helixi38 – 5215Combined sources
Beta strandi55 – 606Combined sources
Helixi63 – 719Combined sources
Beta strandi75 – 828Combined sources
Helixi86 – 927Combined sources
Beta strandi94 – 10815Combined sources
Turni115 – 1173Combined sources
Helixi122 – 1298Combined sources
Helixi139 – 1446Combined sources
Beta strandi156 – 1627Combined sources
Helixi165 – 17713Combined sources
Helixi179 – 1846Combined sources
Beta strandi188 – 1903Combined sources
Beta strandi197 – 2004Combined sources
Helixi201 – 21111Combined sources
Beta strandi215 – 2206Combined sources
Helixi221 – 2255Combined sources
Turni226 – 2283Combined sources
Beta strandi233 – 2353Combined sources
Helixi246 – 2527Combined sources
Turni253 – 2553Combined sources
Turni258 – 2603Combined sources
Beta strandi271 – 2733Combined sources
Beta strandi276 – 28813Combined sources
Helixi292 – 30817Combined sources
Helixi310 – 3156Combined sources
Helixi323 – 33614Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A40X-ray1.70A26-346[»]
1A54X-ray1.60A26-346[»]
1A55X-ray2.40A26-346[»]
1IXGX-ray1.05A26-346[»]
1IXHX-ray0.98A26-346[»]
1IXIX-ray1.89A26-346[»]
1OIBX-ray2.40A/B26-346[»]
1PBPX-ray1.90A26-346[»]
1QUIX-ray1.90A26-346[»]
1QUJX-ray1.90A26-346[»]
1QUKX-ray1.70A26-346[»]
1QULX-ray1.70A26-346[»]
2ABHX-ray1.70A26-346[»]
ProteinModelPortaliP0AG82.
SMRiP0AG82. Positions 26-346.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AG82.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni34 – 363Phosphate bindingBy similarity
Regioni164 – 1663Phosphate bindingBy similarity

Sequence similaritiesi

Belongs to the PstS family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QMV. Bacteria.
COG0226. LUCA.
HOGENOMiHOG000236794.
InParanoidiP0AG82.
KOiK02040.
OMAiGEIEYWD.
OrthoDBiEOG6Z99XR.
PhylomeDBiP0AG82.

Family and domain databases

InterProiIPR005673. ABC_phos-bd_PstS.
IPR024370. PBP_domain.
[Graphical view]
PfamiPF12849. PBP_like_2. 1 hit.
[Graphical view]
PIRSFiPIRSF002756. PstS. 1 hit.
TIGRFAMsiTIGR00975. 3a0107s03. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AG82-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVMRTTVAT VVAATLSMSA FSVFAEASLT GAGATFPAPV YAKWADTYQK
60 70 80 90 100
ETGNKVNYQG IGSSGGVKQI IANTVDFGAS DAPLSDEKLA QEGLFQFPTV
110 120 130 140 150
IGGVVLAVNI PGLKSGELVL DGKTLGDIYL GKIKKWDDEA IAKLNPGLKL
160 170 180 190 200
PSQNIAVVRR ADGSGTSFVF TSYLAKVNEE WKNNVGTGST VKWPIGLGGK
210 220 230 240 250
GNDGIAAFVQ RLPGAIGYVE YAYAKQNNLA YTKLISADGK PVSPTEENFA
260 270 280 290 300
NAAKGADWSK TFAQDLTNQK GEDAWPITST TFILIHKDQK KPEQGTEVLK
310 320 330 340
FFDWAYKTGA KQANDLDYAS LPDSVVEQVR AAWKTNIKDS SGKPLY
Length:346
Mass (Da):37,024
Last modified:January 1, 1988 - v1
Checksum:i867DA7199C2C87ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01992 Genomic DNA. Translation: AAA24378.1.
L10328 Genomic DNA. Translation: AAA62079.1.
U00096 Genomic DNA. Translation: AAC76751.1.
AP009048 Genomic DNA. Translation: BAE77560.1.
PIRiA30277. BYECPR.
RefSeqiNP_418184.1. NC_000913.3.
WP_000867146.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76751; AAC76751; b3728.
BAE77560; BAE77560; BAE77560.
GeneIDi948237.
KEGGiecj:JW3706.
eco:b3728.
PATRICi32122953. VBIEscCol129921_3852.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01992 Genomic DNA. Translation: AAA24378.1.
L10328 Genomic DNA. Translation: AAA62079.1.
U00096 Genomic DNA. Translation: AAC76751.1.
AP009048 Genomic DNA. Translation: BAE77560.1.
PIRiA30277. BYECPR.
RefSeqiNP_418184.1. NC_000913.3.
WP_000867146.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A40X-ray1.70A26-346[»]
1A54X-ray1.60A26-346[»]
1A55X-ray2.40A26-346[»]
1IXGX-ray1.05A26-346[»]
1IXHX-ray0.98A26-346[»]
1IXIX-ray1.89A26-346[»]
1OIBX-ray2.40A/B26-346[»]
1PBPX-ray1.90A26-346[»]
1QUIX-ray1.90A26-346[»]
1QUJX-ray1.90A26-346[»]
1QUKX-ray1.70A26-346[»]
1QULX-ray1.70A26-346[»]
2ABHX-ray1.70A26-346[»]
ProteinModelPortaliP0AG82.
SMRiP0AG82. Positions 26-346.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262139. 6 interactions.
DIPiDIP-48241N.
IntActiP0AG82. 2 interactions.
MINTiMINT-1224444.
STRINGi511145.b3728.

Protein family/group databases

TCDBi3.A.1.7.1. the atp-binding cassette (abc) superfamily.

2D gel databases

SWISS-2DPAGEP0AG82.

Proteomic databases

EPDiP0AG82.
PaxDbiP0AG82.
PRIDEiP0AG82.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76751; AAC76751; b3728.
BAE77560; BAE77560; BAE77560.
GeneIDi948237.
KEGGiecj:JW3706.
eco:b3728.
PATRICi32122953. VBIEscCol129921_3852.

Organism-specific databases

EchoBASEiEB0727.
EcoGeneiEG10734. pstS.

Phylogenomic databases

eggNOGiENOG4107QMV. Bacteria.
COG0226. LUCA.
HOGENOMiHOG000236794.
InParanoidiP0AG82.
KOiK02040.
OMAiGEIEYWD.
OrthoDBiEOG6Z99XR.
PhylomeDBiP0AG82.

Enzyme and pathway databases

BioCyciEcoCyc:PSTS-MONOMER.
ECOL316407:JW3706-MONOMER.
MetaCyc:PSTS-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AG82.
PROiP0AG82.

Family and domain databases

InterProiIPR005673. ABC_phos-bd_PstS.
IPR024370. PBP_domain.
[Graphical view]
PfamiPF12849. PBP_like_2. 1 hit.
[Graphical view]
PIRSFiPIRSF002756. PstS. 1 hit.
TIGRFAMsiTIGR00975. 3a0107s03. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural gene for the phosphate-repressible phosphate-binding protein of Escherichia coli has its own promoter: complete nucleotide sequence of the phoS gene."
    Surin B.P., Jans D.A., Fimmel A.L., Shaw D.C., Cox G.B., Rosenberg H.
    J. Bacteriol. 157:772-778(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  2. "Nucleotide sequence of the phoS gene, the structural gene for the phosphate-binding protein of Escherichia coli."
    Magota K., Otsuji N., Miki T., Horiuchi T., Tsunasawa S., Kondo J., Sakiyama F., Amemura M., Morita T., Shinagawa H., Nakata A.
    J. Bacteriol. 157:909-917(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  3. "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
    Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
    Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
    Link A.J., Robison K., Church G.M.
    Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 26-37.
    Strain: K12 / EMG2.
  7. "High specificity of a phosphate transport protein determined by hydrogen bonds."
    Luecke H., Quiocho F.A.
    Nature 347:402-406(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).
  8. "A low energy short hydrogen bond in very high resolution structures of protein receptor-phosphate complexes."
    Wang Z., Luecke H., Yao N., Quiocho F.A.
    Nat. Struct. Biol. 4:519-522(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.89 ANGSTROMS) OF MUTANT ASN-81.
  9. "Crystal structure of phosphate binding protein labeled with a coumarin fluorophore, a probe for inorganic phosphate."
    Hirshberg M., Henrick K., Haire L.L., Vasisht N., Brune M., Corrie J.E., Webb M.R.
    Biochemistry 37:10381-10385(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

Entry informationi

Entry nameiPSTS_ECOLI
AccessioniPrimary (citable) accession number: P0AG82
Secondary accession number(s): P06128, P76744, Q2M846
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: March 16, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.