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Protein

Phosphate-binding protein PstS

Gene

pstS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex PstSACB involved in phosphate import.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei63PhosphateBy similarity1
Binding sitei81PhosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Phosphate transport, Stress response, Transport

Enzyme and pathway databases

BioCyciEcoCyc:PSTS-MONOMER.
ECOL316407:JW3706-MONOMER.
MetaCyc:PSTS-MONOMER.

Protein family/group databases

TCDBi3.A.1.7.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphate-binding protein PstS
Short name:
PBP
Gene namesi
Name:pstS
Synonyms:phoS
Ordered Locus Names:b3728, JW3706
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10734. pstS.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 251 PublicationAdd BLAST25
ChainiPRO_000003184826 – 346Phosphate-binding protein PstSAdd BLAST321

Proteomic databases

EPDiP0AG82.
PaxDbiP0AG82.
PRIDEiP0AG82.

2D gel databases

SWISS-2DPAGEP0AG82.

Expressioni

Inductioni

By phosphate deprivation. Positively regulated by PhoB and negatively regulated by PhoR.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).Curated

Protein-protein interaction databases

BioGridi4262139. 6 interactors.
DIPiDIP-48241N.
IntActiP0AG82. 2 interactors.
MINTiMINT-1224444.
STRINGi511145.b3728.

Structurei

Secondary structure

1346
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi28 – 33Combined sources6
Beta strandi35 – 37Combined sources3
Helixi38 – 52Combined sources15
Beta strandi55 – 60Combined sources6
Helixi63 – 71Combined sources9
Beta strandi75 – 82Combined sources8
Helixi86 – 92Combined sources7
Beta strandi94 – 108Combined sources15
Turni115 – 117Combined sources3
Helixi122 – 129Combined sources8
Helixi139 – 144Combined sources6
Beta strandi156 – 162Combined sources7
Helixi165 – 177Combined sources13
Helixi179 – 184Combined sources6
Beta strandi188 – 190Combined sources3
Beta strandi197 – 200Combined sources4
Helixi201 – 211Combined sources11
Beta strandi215 – 220Combined sources6
Helixi221 – 225Combined sources5
Turni226 – 228Combined sources3
Beta strandi233 – 235Combined sources3
Helixi246 – 252Combined sources7
Turni253 – 255Combined sources3
Turni258 – 260Combined sources3
Beta strandi271 – 273Combined sources3
Beta strandi276 – 288Combined sources13
Helixi292 – 308Combined sources17
Helixi310 – 315Combined sources6
Helixi323 – 336Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A40X-ray1.70A26-346[»]
1A54X-ray1.60A26-346[»]
1A55X-ray2.40A26-346[»]
1IXGX-ray1.05A26-346[»]
1IXHX-ray0.98A26-346[»]
1IXIX-ray1.89A26-346[»]
1OIBX-ray2.40A/B26-346[»]
1PBPX-ray1.90A26-346[»]
1QUIX-ray1.90A26-346[»]
1QUJX-ray1.90A26-346[»]
1QUKX-ray1.70A26-346[»]
1QULX-ray1.70A26-346[»]
2ABHX-ray1.70A26-346[»]
ProteinModelPortaliP0AG82.
SMRiP0AG82.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AG82.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni34 – 36Phosphate bindingBy similarity3
Regioni164 – 166Phosphate bindingBy similarity3

Sequence similaritiesi

Belongs to the PstS family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QMV. Bacteria.
COG0226. LUCA.
HOGENOMiHOG000236794.
InParanoidiP0AG82.
KOiK02040.
OMAiGEIEYWD.
PhylomeDBiP0AG82.

Family and domain databases

InterProiIPR005673. ABC_phos-bd_PstS.
IPR024370. PBP_domain.
[Graphical view]
PfamiPF12849. PBP_like_2. 1 hit.
[Graphical view]
PIRSFiPIRSF002756. PstS. 1 hit.
TIGRFAMsiTIGR00975. 3a0107s03. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AG82-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVMRTTVAT VVAATLSMSA FSVFAEASLT GAGATFPAPV YAKWADTYQK
60 70 80 90 100
ETGNKVNYQG IGSSGGVKQI IANTVDFGAS DAPLSDEKLA QEGLFQFPTV
110 120 130 140 150
IGGVVLAVNI PGLKSGELVL DGKTLGDIYL GKIKKWDDEA IAKLNPGLKL
160 170 180 190 200
PSQNIAVVRR ADGSGTSFVF TSYLAKVNEE WKNNVGTGST VKWPIGLGGK
210 220 230 240 250
GNDGIAAFVQ RLPGAIGYVE YAYAKQNNLA YTKLISADGK PVSPTEENFA
260 270 280 290 300
NAAKGADWSK TFAQDLTNQK GEDAWPITST TFILIHKDQK KPEQGTEVLK
310 320 330 340
FFDWAYKTGA KQANDLDYAS LPDSVVEQVR AAWKTNIKDS SGKPLY
Length:346
Mass (Da):37,024
Last modified:January 1, 1988 - v1
Checksum:i867DA7199C2C87ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01992 Genomic DNA. Translation: AAA24378.1.
L10328 Genomic DNA. Translation: AAA62079.1.
U00096 Genomic DNA. Translation: AAC76751.1.
AP009048 Genomic DNA. Translation: BAE77560.1.
PIRiA30277. BYECPR.
RefSeqiNP_418184.1. NC_000913.3.
WP_000867146.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76751; AAC76751; b3728.
BAE77560; BAE77560; BAE77560.
GeneIDi948237.
KEGGiecj:JW3706.
eco:b3728.
PATRICi32122953. VBIEscCol129921_3852.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01992 Genomic DNA. Translation: AAA24378.1.
L10328 Genomic DNA. Translation: AAA62079.1.
U00096 Genomic DNA. Translation: AAC76751.1.
AP009048 Genomic DNA. Translation: BAE77560.1.
PIRiA30277. BYECPR.
RefSeqiNP_418184.1. NC_000913.3.
WP_000867146.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A40X-ray1.70A26-346[»]
1A54X-ray1.60A26-346[»]
1A55X-ray2.40A26-346[»]
1IXGX-ray1.05A26-346[»]
1IXHX-ray0.98A26-346[»]
1IXIX-ray1.89A26-346[»]
1OIBX-ray2.40A/B26-346[»]
1PBPX-ray1.90A26-346[»]
1QUIX-ray1.90A26-346[»]
1QUJX-ray1.90A26-346[»]
1QUKX-ray1.70A26-346[»]
1QULX-ray1.70A26-346[»]
2ABHX-ray1.70A26-346[»]
ProteinModelPortaliP0AG82.
SMRiP0AG82.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262139. 6 interactors.
DIPiDIP-48241N.
IntActiP0AG82. 2 interactors.
MINTiMINT-1224444.
STRINGi511145.b3728.

Protein family/group databases

TCDBi3.A.1.7.1. the atp-binding cassette (abc) superfamily.

2D gel databases

SWISS-2DPAGEP0AG82.

Proteomic databases

EPDiP0AG82.
PaxDbiP0AG82.
PRIDEiP0AG82.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76751; AAC76751; b3728.
BAE77560; BAE77560; BAE77560.
GeneIDi948237.
KEGGiecj:JW3706.
eco:b3728.
PATRICi32122953. VBIEscCol129921_3852.

Organism-specific databases

EchoBASEiEB0727.
EcoGeneiEG10734. pstS.

Phylogenomic databases

eggNOGiENOG4107QMV. Bacteria.
COG0226. LUCA.
HOGENOMiHOG000236794.
InParanoidiP0AG82.
KOiK02040.
OMAiGEIEYWD.
PhylomeDBiP0AG82.

Enzyme and pathway databases

BioCyciEcoCyc:PSTS-MONOMER.
ECOL316407:JW3706-MONOMER.
MetaCyc:PSTS-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0AG82.
PROiP0AG82.

Family and domain databases

InterProiIPR005673. ABC_phos-bd_PstS.
IPR024370. PBP_domain.
[Graphical view]
PfamiPF12849. PBP_like_2. 1 hit.
[Graphical view]
PIRSFiPIRSF002756. PstS. 1 hit.
TIGRFAMsiTIGR00975. 3a0107s03. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPSTS_ECOLI
AccessioniPrimary (citable) accession number: P0AG82
Secondary accession number(s): P06128, P76744, Q2M846
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 30, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.