Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nuclease SbcCD subunit D

Gene

sbcD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand endonuclease activity.

GO - Molecular functioni

GO - Biological processi

  • DNA metabolic process Source: EcoCyc
  • DNA recombination Source: UniProtKB-KW
  • DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA recombination, DNA replication

Enzyme and pathway databases

BioCyciEcoCyc:EG11094-MONOMER.
ECOL316407:JW0388-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclease SbcCD subunit D
Gene namesi
Name:sbcD
Synonyms:yajA
Ordered Locus Names:b0398, JW0388
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11094. sbcD.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400Nuclease SbcCD subunit DPRO_0000182195Add
BLAST

Proteomic databases

PaxDbiP0AG76.

Interactioni

Subunit structurei

Heterodimer of SbcC and SbcD.

Protein-protein interaction databases

BioGridi4262090. 136 interactions.
DIPiDIP-10828N.
IntActiP0AG76. 5 interactions.
STRINGi511145.b0398.

Structurei

3D structure databases

ProteinModelPortaliP0AG76.
SMRiP0AG76. Positions 1-386.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SbcD family.Curated

Phylogenomic databases

eggNOGiENOG4107RH8. Bacteria.
COG0420. LUCA.
HOGENOMiHOG000026261.
InParanoidiP0AG76.
KOiK03547.
OMAiDAFDTVN.
OrthoDBiEOG60CWP4.
PhylomeDBiP0AG76.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR004593. SbcD.
IPR026843. SbcD_C.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF12320. SbcD_C. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
TIGRFAMsiTIGR00619. sbcd. 1 hit.

Sequencei

Sequence statusi: Complete.

P0AG76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRILHTSDWH LGQNFYSKSR EAEHQAFLDW LLETAQTHQV DAIIVAGDVF
60 70 80 90 100
DTGSPPSYAR TLYNRFVVNL QQTGCHLVVL AGNHDSVATL NESRDIMAFL
110 120 130 140 150
NTTVVASAGH APQILPRRDG TPGAVLCPIP FLRPRDIITS QAGLNGIEKQ
160 170 180 190 200
QHLLAAITDY YQQHYADACK LRGDQPLPII ATGHLTTVGA SKSDAVRDIY
210 220 230 240 250
IGTLDAFPAQ NFPPADYIAL GHIHRAQIIG GMEHVRYCGS PIPLSFDECG
260 270 280 290 300
KSKYVHLVTF SNGKLESVEN LNVPVTQPMA VLKGDLASIT AQLEQWRDVS
310 320 330 340 350
QEPPVWLDIE ITTDEYLHDI QRKIQALTES LPVEVLLVRR SREQRERVLA
360 370 380 390 400
SQQRETLSEL SVEEVFNRRL ALEELDESQQ QRLQHLFTTT LHTLAGEHEA
Length:400
Mass (Da):44,714
Last modified:December 20, 2005 - v1
Checksum:iE8894089198C6D5B
GO

Sequence cautioni

The sequence AAB18122.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15981 Genomic DNA. Translation: CAA34103.1.
U73857 Genomic DNA. Translation: AAB18122.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73501.1.
AP009048 Genomic DNA. Translation: BAE76178.1.
PIRiJS0349.
RefSeqiNP_414932.1. NC_000913.3.
WP_001221319.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73501; AAC73501; b0398.
BAE76178; BAE76178; BAE76178.
GeneIDi945049.
KEGGiecj:JW0388.
eco:b0398.
PATRICi32115939. VBIEscCol129921_0411.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15981 Genomic DNA. Translation: CAA34103.1.
U73857 Genomic DNA. Translation: AAB18122.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73501.1.
AP009048 Genomic DNA. Translation: BAE76178.1.
PIRiJS0349.
RefSeqiNP_414932.1. NC_000913.3.
WP_001221319.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0AG76.
SMRiP0AG76. Positions 1-386.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262090. 136 interactions.
DIPiDIP-10828N.
IntActiP0AG76. 5 interactions.
STRINGi511145.b0398.

Proteomic databases

PaxDbiP0AG76.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73501; AAC73501; b0398.
BAE76178; BAE76178; BAE76178.
GeneIDi945049.
KEGGiecj:JW0388.
eco:b0398.
PATRICi32115939. VBIEscCol129921_0411.

Organism-specific databases

EchoBASEiEB1086.
EcoGeneiEG11094. sbcD.

Phylogenomic databases

eggNOGiENOG4107RH8. Bacteria.
COG0420. LUCA.
HOGENOMiHOG000026261.
InParanoidiP0AG76.
KOiK03547.
OMAiDAFDTVN.
OrthoDBiEOG60CWP4.
PhylomeDBiP0AG76.

Enzyme and pathway databases

BioCyciEcoCyc:EG11094-MONOMER.
ECOL316407:JW0388-MONOMER.

Miscellaneous databases

PROiP0AG76.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR004593. SbcD.
IPR026843. SbcD_C.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF12320. SbcD_C. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
TIGRFAMsiTIGR00619. sbcd. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular organization of sbcC, a gene that affects genetic recombination and the viability of DNA palindromes in Escherichia coli K-12."
    Naom I.S., Morton S.J., Leach D.R.F., Lloyd R.G.
    Nucleic Acids Res. 17:8033-8046(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Identification of sbcD mutations as cosuppressors of recBC that allow propagation of DNA palindromes in Escherichia coli K-12."
    Gibson F.P., Leach D.R.F., Lloyd R.G.
    J. Bacteriol. 174:1222-1228(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "The SbcCD protein of Escherichia coli is related to two putative nucleases in the UvrA superfamily of nucleotide-binding proteins."
    Leach D.R.F., Lloyd R.G., Coulson A.F.
    Genetica 87:95-100(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. "The SbcCD nuclease of Escherichia coli is a structural maintenance of chromosomes (SMC) family protein that cleaves hairpin DNA."
    Connelly J.C., Kirkham L.A., Leach D.R.
    Proc. Natl. Acad. Sci. U.S.A. 95:7969-7974(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  8. "DNA cleavage and degradation by the SbcCD protein complex from Escherichia coli."
    Connelly J.C., de Leau E.S., Leach D.R.
    Nucleic Acids Res. 27:1039-1046(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiSBCD_ECOLI
AccessioniPrimary (citable) accession number: P0AG76
Secondary accession number(s): P13457, Q2MC28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: April 13, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.