P0AG75 (RUSA_ECOL6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Crossover junction endodeoxyribonuclease RusA
EC=3.1.22.4

Alternative name(s):
Holliday junction nuclease RusA
Holliday junction resolvase

Gene names

Name:	rusA
Ordered Locus Names:	c1557

Organism

Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

199310 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	120 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity at the 5' side of CC dinucleotides. Corrects the defects in genetic recombination and DNA repair associated with inactivation of RuvAB or RuvC By similarity.
Catalytic activity	Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the RusA family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair
Ligand	Magnesium Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	crossover junction endodeoxyribonuclease activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 120	120	Crossover junction endodeoxyribonuclease RusA		PRO_0000192005
Regions
	Region	13 – 16	4	DNA binding By similarity
	Region	66 – 73	8	DNA binding By similarity
Sites
	Metal binding	70	1	Magnesium By similarity
	Metal binding	72	1	Magnesium By similarity
	Metal binding	91	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0AG75 [UniParc]. Last modified December 20, 2005. Version 1. Checksum: 38401669E8819EA6	FASTA	120	13,846
	10 20 30 40 50 60 MNTYSITLPW PPSNNRYYRH NRGRTHVSAE GQAYRDNVAR IIKNAMLDIG LAMPVKIRIE 70 80 90 100 110 120 CHMPDRRRRD LDNLQKAAFD ALTKAGFWLD DAQVVDYRVV KMPVTKGGRL ELTITEMGNE

References

[1]

"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli." Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R. Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CFT073 / ATCC 700928 / UPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014075 Genomic DNA. Translation: AAN80026.1.
RefSeq	NP_753466.1. NC_004431.1.
3D structure databases
ProteinModelPortal	P0AG75.
SMR	P0AG75. Positions 1-118.
ModBase	Search...
Protein-protein interaction databases
STRING	199310.c1557.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN80026; AAN80026; c1557.
GeneID	1035306.
KEGG	ecc:c1557.
PATRIC	18281059. VBIEscCol75197_1451.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000119986.
KO	K01160.
OMA	GIHYISD.
ProtClustDB	PRK09786.
Family and domain databases
Gene3D	3.30.1330.70. 1 hit.
InterPro	IPR016281. Endonuclease_RusA. IPR008822. Endonuclease_RusA-like. [Graphical view]
Pfam	PF05866. RusA. 1 hit. [Graphical view]
PIRSF	PIRSF001007. RusA. 1 hit.
SUPFAM	SSF103084. RusA-like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RUSA_ECOL6

Accession

Primary (citable) accession number: P0AG75
Secondary accession number(s): P40116

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	December 20, 2005
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program