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P0AG75 (RUSA_ECOL6) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RusA

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RusA
Holliday junction resolvase
Gene names
Name:rusA
Ordered Locus Names:c1557
OrganismEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) [Complete proteome] [HAMAP]
Taxonomic identifier199310 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length120 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Endonuclease that resolves Holliday junction intermediates made during homologous genetic recombination and DNA repair. Exhibits sequence and structure-selective cleavage of four-way DNA junctions, where it introduces symmetrical nicks in two strands of the same polarity at the 5' side of CC dinucleotides. Corrects the defects in genetic recombination and DNA repair associated with inactivation of RuvAB or RuvC By similarity.

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the RusA family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 120120Crossover junction endodeoxyribonuclease RusA
PRO_0000192005

Regions

Region13 – 164DNA binding By similarity
Region66 – 738DNA binding By similarity

Sites

Metal binding701Magnesium By similarity
Metal binding721Magnesium By similarity
Metal binding911Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
P0AG75 [UniParc].

Last modified December 20, 2005. Version 1.
Checksum: 38401669E8819EA6

FASTA12013,846
        10         20         30         40         50         60 
MNTYSITLPW PPSNNRYYRH NRGRTHVSAE GQAYRDNVAR IIKNAMLDIG LAMPVKIRIE 

        70         80         90        100        110        120 
CHMPDRRRRD LDNLQKAAFD ALTKAGFWLD DAQVVDYRVV KMPVTKGGRL ELTITEMGNE 

« Hide

References

[1]"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli."
Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R.
Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CFT073 / ATCC 700928 / UPEC.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014075 Genomic DNA. Translation: AAN80026.1.
RefSeqNP_753466.1. NC_004431.1.

3D structure databases

ProteinModelPortalP0AG75.
SMRP0AG75. Positions 1-118.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING199310.c1557.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN80026; AAN80026; c1557.
GeneID1035306.
KEGGecc:c1557.
PATRIC18281059. VBIEscCol75197_1451.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000119986.
KOK01160.
OMARGIHYIS.
OrthoDBEOG66HVH9.
ProtClustDBPRK09786.

Enzyme and pathway databases

BioCycECOL199310:C1557-MONOMER.

Family and domain databases

Gene3D3.30.1330.70. 1 hit.
InterProIPR016281. Endonuclease_RusA.
IPR008822. Endonuclease_RusA-like.
[Graphical view]
PfamPF05866. RusA. 1 hit.
[Graphical view]
PIRSFPIRSF001007. RusA. 1 hit.
SUPFAMSSF103084. SSF103084. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRUSA_ECOL6
AccessionPrimary (citable) accession number: P0AG75
Secondary accession number(s): P40116
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: April 16, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families